| dbo:abstract |
Ein Aldehyd-Tag ist ein Protein-Tag mit einer Aldehydgruppe, das in der Biochemie zur Proteincharakterisierung verwendet wird. (de) The aldehyde tag is a short peptide tag that can be further modified to add fluorophores, glycans, PEG (polyethylene glycol) chains or reactive groups for further synthesis. A short, genetically-encoded peptide, with a consensus sequence LCxPxR, is introduced into fusion proteins, and by subsequent treatment with the formylglycine-generating enzyme (FGE), the cysteine of the tag is converted to a reactive aldehyde group. This electrophilic group can be targeted by an array of aldehyde-specific reagents, such as aminooxy- or hydrazide-functionalized compounds. (en) |
| dbo:thumbnail |
wiki-commons:Special:FilePath/Conjugations_formylglycine.png?width=300 |
| dbo:wikiPageExternalLink |
http://www.rcsb.org/pdb/explore.do%3FstructureId=1hgu http://www.rcsb.org/pdb/explore/explore.do%3FstructureId=1IGT https://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi%3Fuid=48794 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3379715/figure/fig03/ |
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dbr:Peptide dbr:Mass_spectrometry dbr:Fusion_protein dbr:Cysteine dbr:E._coli dbr:Fluorophore dbr:Formylglycine-generating_enzyme dbr:Glycan dbr:Hydrazide dbr:Hydrazone dbc:Peptides dbc:Biochemical_separation_processes dbr:Aldehyde dbr:Bioconjugation dbr:Bioorthogonal_chemical_reporter dbr:Bioorthogonal_chemistry dbr:Pictet–Spengler_reaction dbr:Polyethylene_glycol dbr:His-tag dbr:Primary_sequence dbr:Schiff_base dbr:Expanded_genetic_code dbr:Sulfatase dbr:Sulfenic_acid dbr:Oxime dbr:Posttranslational_modification dbr:Formyl_group dbr:Β-elimination dbr:Thioaldehyde dbr:Thiol_group dbr:File:Chemical_glycosylation_FC_fragment.png dbr:File:Conjugations_formylglycine.png dbr:File:FGE_mechansim.tif dbr:File:HGH_2Ang_marineblue_bgwhite.tif dbr:File:IgG_conjugate_1.png |
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dbt:Advert dbt:Multiple_issues dbt:Primary_sources dbt:Reflist dbt:Technical |
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dbc:Peptides dbc:Biochemical_separation_processes |
| gold:hypernym |
dbr:Tag |
| rdf:type |
dbo:Person |
| rdfs:comment |
Ein Aldehyd-Tag ist ein Protein-Tag mit einer Aldehydgruppe, das in der Biochemie zur Proteincharakterisierung verwendet wird. (de) The aldehyde tag is a short peptide tag that can be further modified to add fluorophores, glycans, PEG (polyethylene glycol) chains or reactive groups for further synthesis. A short, genetically-encoded peptide, with a consensus sequence LCxPxR, is introduced into fusion proteins, and by subsequent treatment with the formylglycine-generating enzyme (FGE), the cysteine of the tag is converted to a reactive aldehyde group. This electrophilic group can be targeted by an array of aldehyde-specific reagents, such as aminooxy- or hydrazide-functionalized compounds. (en) |
| rdfs:label |
Aldehyd-Tag (de) Aldehyde tag (en) |
| owl:sameAs |
freebase:Aldehyde tag yago-res:Aldehyde tag wikidata:Aldehyde tag dbpedia-de:Aldehyde tag https://global.dbpedia.org/id/oT7L |
| prov:wasDerivedFrom |
wikipedia-en:Aldehyde_tag?oldid=1120570826&ns=0 |
| foaf:depiction |
wiki-commons:Special:FilePath/Chemical_glycosylation_FC_fragment.png wiki-commons:Special:FilePath/Conjugations_formylglycine.png wiki-commons:Special:FilePath/IgG_conjugate_1.png |
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wikipedia-en:Aldehyde_tag |
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wikipedia-en:Aldehyde_tag |
