| dbo:abstract |
Fura-2-AM ist ein membrangängiges Derivat des Fura-2. Fura-2 wurde 1985 von Roger Tsien und Kollegen entwickelt und dient als Ca2+-Indikator. (de) Fura-2-acetoxymethyl ester, often abbreviated Fura-2AM, is a membrane-permeant derivative of the ratiometric calcium indicator Fura-2 used in biochemistry to measure cellular calcium concentrations by fluorescence. When added to cells, Fura-2AM crosses cell membranes and once inside the cell, the acetoxymethyl groups are removed by cellular esterases. Removal of the acetoxymethyl esters regenerates "Fura-2", the pentacarboxylate calcium indicator. Measurement of Ca2+-induced fluorescence at both 340 nm and 380 nm allows for calculation of calcium concentrations based 340/380 ratios. The use of the ratio automatically cancels out certain variables such as local differences in fura-2 concentration or cell thickness that would otherwise lead to artifacts when attempting to image calcium concentrations in cells. (en) |
| dbo:alternativeName |
Fura-2AM (en) |
| dbo:iupacName |
(Acetyloxy)methyl 2-(5-{2-[5-methyl-2-(2,6,10,14-tetraoxo-3,5,11,13-tetraoxa-8-azapentadecan-8-yl)phenoxy]ethoxy}-6-(2,6,10,14-tetraoxo-3,5,11,13-tetraoxa-8-azapentadecan-8-yl)-1-benzofuran-2-yl)-1,3-oxazole-5-carboxylate (en) |
| dbo:thumbnail |
wiki-commons:Special:FilePath/Fura_2AM.png?width=300 |
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4128785 (xsd:integer) |
| dbo:wikiPageLength |
3179 (xsd:nonNegativeInteger) |
| dbo:wikiPageRevisionID |
1059474931 (xsd:integer) |
| dbo:wikiPageWikiLink |
dbr:Calcium dbc:Acetate_esters dbc:Fluorescent_dyes dbr:Fura-2 dbc:Formals dbc:Benzofuran_ethers_at_the_benzene_ring dbc:Cell_imaging dbc:Cell_culture_reagents dbr:Fluorescence dbc:Oxazoles dbc:Biochemistry_methods |
| dbp:imagefile |
Fura 2AM.png (en) |
| dbp:imagesize |
200 (xsd:integer) |
| dbp:othernames |
Fura-2AM (en) |
| dbp:pin |
methyl 2--1,3-oxazole-5-carboxylate (en) |
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427818427 (xsd:integer) |
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dbt:Biochem-stub dbt:Chembox dbt:Chembox_Hazards dbt:Chembox_Identifiers dbt:Chembox_Properties dbt:Reflist dbt:Cascite dbt:Chemspidercite dbt:Stdinchicite |
| dct:subject |
dbc:Acetate_esters dbc:Fluorescent_dyes dbc:Formals dbc:Benzofuran_ethers_at_the_benzene_ring dbc:Cell_imaging dbc:Cell_culture_reagents dbc:Oxazoles dbc:Biochemistry_methods |
| gold:hypernym |
dbr:Derivative |
| rdf:type |
owl:Thing dul:ChemicalObject dbo:ChemicalSubstance wikidata:Q11173 yago:WikicatBenzofurans yago:WikicatBiochemistryMethods yago:WikicatCarboxylateEsters yago:Ability105616246 yago:AbsorptionIndicator114598383 yago:Abstraction100002137 yago:Benzofuran114898101 yago:Chemical114806838 yago:Cognition100023271 yago:ColoringMaterial114984973 yago:Compound114818238 yago:Dye114985383 yago:Element114840755 yago:Ester114850483 yago:Fluorescein114987695 yago:Indicator114917208 yago:Know-how105616786 yago:Material114580897 yago:Matter100020827 yago:Method105660268 yago:OrganicCompound114727670 yago:Part113809207 yago:PhysicalEntity100001930 yago:PsychologicalFeature100023100 yago:Quintessence114847103 yago:Relation100031921 dbo:ChemicalCompound yago:Substance100019613 yago:WikicatAcetateEsters yago:WikicatFluorescentDyes yago:WikicatPhenolEthers umbel-rc:ChemicalSubstanceType |
| rdfs:comment |
Fura-2-AM ist ein membrangängiges Derivat des Fura-2. Fura-2 wurde 1985 von Roger Tsien und Kollegen entwickelt und dient als Ca2+-Indikator. (de) Fura-2-acetoxymethyl ester, often abbreviated Fura-2AM, is a membrane-permeant derivative of the ratiometric calcium indicator Fura-2 used in biochemistry to measure cellular calcium concentrations by fluorescence. When added to cells, Fura-2AM crosses cell membranes and once inside the cell, the acetoxymethyl groups are removed by cellular esterases. Removal of the acetoxymethyl esters regenerates "Fura-2", the pentacarboxylate calcium indicator. Measurement of Ca2+-induced fluorescence at both 340 nm and 380 nm allows for calculation of calcium concentrations based 340/380 ratios. The use of the ratio automatically cancels out certain variables such as local differences in fura-2 concentration or cell thickness that would otherwise lead to artifacts when attempting to image calcium conce (en) |
| rdfs:label |
Fura-2AM (de) Fura-2-acetoxymethyl ester (en) |
| owl:sameAs |
freebase:Fura-2-acetoxymethyl ester yago-res:Fura-2-acetoxymethyl ester wikidata:Fura-2-acetoxymethyl ester http://azb.dbpedia.org/resource/فورا-۲-استوکسیومتیل_استر dbpedia-de:Fura-2-acetoxymethyl ester dbpedia-fa:Fura-2-acetoxymethyl ester dbpedia-sh:Fura-2-acetoxymethyl ester dbpedia-sr:Fura-2-acetoxymethyl ester https://global.dbpedia.org/id/3vygo |
| prov:wasDerivedFrom |
wikipedia-en:Fura-2-acetoxymethyl_ester?oldid=1059474931&ns=0 |
| foaf:depiction |
wiki-commons:Special:FilePath/Fura_2AM.png |
| foaf:isPrimaryTopicOf |
wikipedia-en:Fura-2-acetoxymethyl_ester |
| is dbo:wikiPageDisambiguates of |
dbr:Fura |
| is dbo:wikiPageRedirects of |
dbr:Fura-2-acetoxymethyl dbr:Fura-2/AM dbr:Fura-2AM dbr:Fura-2am dbr:Fura_2/AM dbr:Fura_2AM dbr:Fura_2am |
| is dbo:wikiPageWikiLink of |
dbr:Fura dbr:Fura-2-acetoxymethyl dbr:Fura-2/AM dbr:Fura-2AM dbr:Fura-2am dbr:Fura_2/AM dbr:Fura_2AM dbr:Fura_2am |
| is foaf:primaryTopic of |
wikipedia-en:Fura-2-acetoxymethyl_ester |
