Hydrophilic interaction chromatography (original) (raw)
Hydrophilic interaction chromatography (or hydrophilic interaction liquid chromatography, HILIC) is a variant of normal phase liquid chromatography that partly overlaps with other chromatographic applications such as ion chromatography and reversed phase liquid chromatography. HILIC uses hydrophilic stationary phases with reversed-phase type eluents. The name was suggested by Andrew Alpert in his 1990 paper on the subject. He described the chromatographic mechanism for it as liquid-liquid partition chromatography where analytes elute in order of increasing polarity, a conclusion supported by a review and re-evaluation of published data.
| Property | Value |
|---|---|
| dbo:abstract | Hydrophilic interaction chromatography (or hydrophilic interaction liquid chromatography, HILIC) is a variant of normal phase liquid chromatography that partly overlaps with other chromatographic applications such as ion chromatography and reversed phase liquid chromatography. HILIC uses hydrophilic stationary phases with reversed-phase type eluents. The name was suggested by Andrew Alpert in his 1990 paper on the subject. He described the chromatographic mechanism for it as liquid-liquid partition chromatography where analytes elute in order of increasing polarity, a conclusion supported by a review and re-evaluation of published data. (en) La chromatographie à interaction hydrophile (HILIC) est une technique de chromatographie qui permet la séparation de petites molécules polaires. Elle requiert l'utilisation d'une phase mobile constituée d'un solvant organique miscible dans l'eau, souvent l'acétonitrile, et d'un tampon aqueux. La séparation est le résultat d'un partage ou une adsorption de l'analyte dans une couche enrichie d'eau sur la phase stationnaire polaire. (fr) Hydrophilic Interaction Liquid Chromatography (hydrofiele interactie-vloeistofchromatografie), meestal afgekort tot HILIC, is een vorm van Normal Phase Liquid Chromatography (NPLC) (normale fase-vloeistofchromatografie). Andere namen zijn Omgekeerde RPLC (RPLC = Reversed Phase Liquid Chromatography/omgekeerde fase-vloestofchromatografie) of waterige normale fase-chromatografie. Andrew Alpert gaf deze methode de naam HILIC om verwarring met NPLC te voorkomen.Door middel van HILIC worden sterk polaire en hydrofiele verbindingen, die weinig retentie hebben bij een omgekeerde fase-kolom, gescheiden. De retentie van HILIC-kolommen voor sterk polaire basen is hoger dan die van de omgekeerde fase-kolommen.In tegenstelling tot NPLC, kan men bij HILIC gebruikmaken van oplosmiddelen die niet met water gemengd kunnen worden. (nl) |
| dbo:thumbnail | wiki-commons:Special:FilePath/HILIC_Partition_Method_Graphic.png?width=300 |
| dbo:wikiPageID | 7150276 (xsd:integer) |
| dbo:wikiPageLength | 13567 (xsd:nonNegativeInteger) |
| dbo:wikiPageRevisionID | 1069217625 (xsd:integer) |
| dbo:wikiPageWikiLink | dbr:Betaine dbr:Inorganic_compound dbr:Protein_isoform dbr:Analyte dbr:Anionic dbr:Elution dbr:Organic_compound dbr:Gentamicin dbr:Silanol dbr:Zwitterion dbr:Stationary_phase_(chemistry) dbr:Ion_chromatography dbr:Sulfonate dbr:Acetonitrile dbr:Adenosine_triphosphate dbr:Amide dbr:Ammonium_acetate dbc:Molecular_biology dbr:Amino dbr:PH dbr:Biologic_medical_product dbr:Glycoprotein dbr:Glycosylation dbr:Reversed-phase_chromatography dbr:High-performance_liquid_chromatography dbr:Ionic_compound dbc:Biochemistry_methods dbc:Chromatography dbc:Laboratory_techniques dbr:Chemical_polarity dbr:Biomolecule dbr:Sulfonic_acid dbr:THF dbr:Standard_Gibbs_function_of_formation dbr:Aqueous dbr:Aqueous_Normal_Phase_Chromatography dbr:Polymer dbr:Ion_exchange_chromatography dbr:Cationic dbr:Dioxane dbr:Polar_compound dbr:Mobile_phase dbr:File:HILIC_Partition_Method_Graphic.png |
| dbp:wikiPageUsesTemplate | dbt:COI dbt:Reflist dbt:See_also dbt:Short_description |
| dct:subject | dbc:Molecular_biology dbc:Biochemistry_methods dbc:Chromatography dbc:Laboratory_techniques |
| gold:hypernym | dbr:Variant |
| rdf:type | owl:Thing yago:WikicatBiochemistryMethods yago:WikicatLaboratoryTechniques yago:Ability105616246 yago:Abstraction100002137 yago:Cognition100023271 yago:Know-how105616786 yago:Method105660268 yago:PsychologicalFeature100023100 yago:Technique105665146 |
| rdfs:comment | Hydrophilic interaction chromatography (or hydrophilic interaction liquid chromatography, HILIC) is a variant of normal phase liquid chromatography that partly overlaps with other chromatographic applications such as ion chromatography and reversed phase liquid chromatography. HILIC uses hydrophilic stationary phases with reversed-phase type eluents. The name was suggested by Andrew Alpert in his 1990 paper on the subject. He described the chromatographic mechanism for it as liquid-liquid partition chromatography where analytes elute in order of increasing polarity, a conclusion supported by a review and re-evaluation of published data. (en) La chromatographie à interaction hydrophile (HILIC) est une technique de chromatographie qui permet la séparation de petites molécules polaires. Elle requiert l'utilisation d'une phase mobile constituée d'un solvant organique miscible dans l'eau, souvent l'acétonitrile, et d'un tampon aqueux. La séparation est le résultat d'un partage ou une adsorption de l'analyte dans une couche enrichie d'eau sur la phase stationnaire polaire. (fr) Hydrophilic Interaction Liquid Chromatography (hydrofiele interactie-vloeistofchromatografie), meestal afgekort tot HILIC, is een vorm van Normal Phase Liquid Chromatography (NPLC) (normale fase-vloeistofchromatografie). Andere namen zijn Omgekeerde RPLC (RPLC = Reversed Phase Liquid Chromatography/omgekeerde fase-vloestofchromatografie) of waterige normale fase-chromatografie. (nl) |
| rdfs:label | Hydrophilic interaction chromatography (en) Chromatographie d'interaction hydrophile (fr) HILIC (nl) |
| rdfs:seeAlso | dbr:Chromatography |
| owl:sameAs | freebase:Hydrophilic interaction chromatography yago-res:Hydrophilic interaction chromatography wikidata:Hydrophilic interaction chromatography dbpedia-fr:Hydrophilic interaction chromatography dbpedia-nl:Hydrophilic interaction chromatography https://global.dbpedia.org/id/4nSG6 |
| prov:wasDerivedFrom | wikipedia-en:Hydrophilic_interaction_chromatography?oldid=1069217625&ns=0 |
| foaf:depiction | wiki-commons:Special:FilePath/HILIC_Partition_Method_Graphic.png |
| foaf:isPrimaryTopicOf | wikipedia-en:Hydrophilic_interaction_chromatography |
| is dbo:wikiPageRedirects of | dbr:Hydrophilic_interaction_liquid_chromatography dbr:HILIC dbr:Hilic |
| is dbo:wikiPageWikiLink of | dbr:N-Acetyltaurine dbr:Biopolymer dbr:Shodex dbr:Exometabolomics dbr:Hydrophilic_interaction_liquid_chromatography dbr:HILIC dbr:Hilic |
| is foaf:primaryTopic of | wikipedia-en:Hydrophilic_interaction_chromatography |
