Jaffe reaction (original) (raw)
Additional information needed The Jaffe reaction is a colorimetric method used in clinical chemistry to determine creatinine levels in blood and urine. In 1886, Max Jaffe (1841–1911) wrote about its basic principles in the paper Über den Niederschlag, welchen Pikrinsäure in normalem Harn erzeugt und über eine neue Reaction des Kreatinins in which he described the properties of creatinine and picric acid in an alkaline solution. The color change that occurred was directly proportional to the concentration of creatinine, however he also noted that several other organic compounds induced similar reactions. In the early 20th century, Otto Folin adapted Jaffe's research into a clinical procedure. The Jaffe reaction, despite its nonspecificity for creatinine, is still widely employed as the meth
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| dbo:abstract | Additional information needed The Jaffe reaction is a colorimetric method used in clinical chemistry to determine creatinine levels in blood and urine. In 1886, Max Jaffe (1841–1911) wrote about its basic principles in the paper Über den Niederschlag, welchen Pikrinsäure in normalem Harn erzeugt und über eine neue Reaction des Kreatinins in which he described the properties of creatinine and picric acid in an alkaline solution. The color change that occurred was directly proportional to the concentration of creatinine, however he also noted that several other organic compounds induced similar reactions. In the early 20th century, Otto Folin adapted Jaffe's research into a clinical procedure. The Jaffe reaction, despite its nonspecificity for creatinine, is still widely employed as the method of choice for creatinine testing due to its speed, adaptability in automated analysis, and cost-effectiveness, and is the oldest methodology continued to be used in the medical laboratory. It is this nonspecificity that has motivated the development of new reference methods for creatinine analysis into the 21st century. (en) Reakcja Jaffego – metoda kolorymetrycznego oznaczania kreatyniny w płynach ustrojowych, wykorzystywana w laboratoriach klinicznych. Barwna reakcja kreatyniny z kwasem pikrynowym (2,3,6-trinitrofenolem) w środowisku alkalicznym została opisana przez w 1886 roku w pracy zatytułowanej Über den Niederschlag, welchen Pikrinsäure in normalem Harn erzeugt und über eine neue Reaction des Kreatinins. W 1914 roku i J.L. Morris opracowali metodę oznaczania kreatyniny z wykorzystaniem reakcji Jaffego w moczu, a w 1919 roku Folin i Hsien Wu w odbiałczonej krwi. Metoda Jaffego polega na reakcji kreatyniny z kwasem pikrynowym (2,3,6-trinitrofenolem) w środowisku alkalicznym. Struktura pomarańczowoczerwonego produktu reakcji była przedmiotem wielu badań. W 1975 roku Anthony R. Butler wykazał, że powstającym barwnym związkiem jest tzw. , którego stężenie można określić mierząc absorbancję przy 483 nm. Stężenie kreatyniny w próbce określa się mierząc absorbancję po reakcji. Reakcja Jaffego, po udoskonaleniech, pozostała ważną metodą analityczną oznaczania kreatyniny w próbkach fizjologicznych. Została opracowana także jej wersja zminiaturyzowana, przeprowadzana na papierku analitycznym, z określaniem stężenia kreatyniny na podstawie pomiaru intensywności RGB powstałego kompleksu Janovsky’ego za pomocą kamery CMOS. (pl) |
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| rdfs:comment | Additional information needed The Jaffe reaction is a colorimetric method used in clinical chemistry to determine creatinine levels in blood and urine. In 1886, Max Jaffe (1841–1911) wrote about its basic principles in the paper Über den Niederschlag, welchen Pikrinsäure in normalem Harn erzeugt und über eine neue Reaction des Kreatinins in which he described the properties of creatinine and picric acid in an alkaline solution. The color change that occurred was directly proportional to the concentration of creatinine, however he also noted that several other organic compounds induced similar reactions. In the early 20th century, Otto Folin adapted Jaffe's research into a clinical procedure. The Jaffe reaction, despite its nonspecificity for creatinine, is still widely employed as the meth (en) Reakcja Jaffego – metoda kolorymetrycznego oznaczania kreatyniny w płynach ustrojowych, wykorzystywana w laboratoriach klinicznych. Barwna reakcja kreatyniny z kwasem pikrynowym (2,3,6-trinitrofenolem) w środowisku alkalicznym została opisana przez w 1886 roku w pracy zatytułowanej Über den Niederschlag, welchen Pikrinsäure in normalem Harn erzeugt und über eine neue Reaction des Kreatinins. W 1914 roku i J.L. Morris opracowali metodę oznaczania kreatyniny z wykorzystaniem reakcji Jaffego w moczu, a w 1919 roku Folin i Hsien Wu w odbiałczonej krwi. Metoda Jaffego polega na reakcji kreatyniny z kwasem pikrynowym (2,3,6-trinitrofenolem) w środowisku alkalicznym. Struktura pomarańczowoczerwonego produktu reakcji była przedmiotem wielu badań. W 1975 roku Anthony R. Butler wykazał, że powstaj (pl) |
| rdfs:label | Jaffe reaction (en) Reakcja Jaffego (pl) |
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