Masson's trichrome stain (original) (raw)
Die Masson-Trichrom-Färbung ist eine histologische Trichrom-Färbungsmethode.
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| dbo:abstract | Die Masson-Trichrom-Färbung ist eine histologische Trichrom-Färbungsmethode. (de) La tinción tricrómica de Masson, al igual que otras tinciones tricrómicas, es una técnica de coloración especial que permite visualizar claramente las fibras de colágeno tipo I que forman fibras gruesas o haces, diseñados para dar resistencia; también evidencia, aunque en menor intensidad, las . Se emplean tres colorantes para diferenciar el núcleo celular, el citoplasma y las fibras de colágeno. (es) Masson's trichrome is a three-colour staining procedure used in histology. The recipes evolved from Claude L. Pierre Masson's (1880–1959) original formulation have different specific applications, but all are suited for distinguishing cells from surrounding connective tissue. Most recipes produce red keratin and muscle fibers, blue or green collagen and bone, light red or pink cytoplasm, and dark brown to black cell nuclei. The trichrome is applied by immersion of the fixated sample into Weigert's iron hematoxylin, and then three different solutions, labeled A, B, and C: * Weigert's hematoxylin is a sequence of three solutions: ferric chloride in diluted hydrochloric acid, hematoxylin in 95% ethanol, and potassium ferricyanide solution alkalized by sodium borate. It is used to stain the nuclei. * Solution A, also called plasma stain, contains acid fuchsin, Xylidine Ponceau, glacial acetic acid, and distilled water. Other red acid dyes can be used, e.g. the Biebrich scarlet in Lillie's trichrome. * Solution B contains phosphomolybdic acid in distilled water. * Solution C, also called fibre stain, contains Light Green SF yellowish, or alternatively Fast Green FCF. It is used to stain collagen. If blue is preferred to green, methyl blue or water blue can be substituted. Standard applications:Masson's trichrome staining is widely used to study muscular pathologies (muscular dystrophy), cardiac pathologies (infarct), hepatic pathologies (cirrhosis) or kidney pathologies (glomerular fibrosis). It can also be used to detect and analyze tumors on hepatic and kidney biopsies. (en) Corante ou coloração tricromo ou tricrômica de Masson é uma combinação de corantes usados em um protocolo de coloração de três cores em histologia. As formulações desta coloração envolvem a formulação original de Masson para diferentes aplicações, mas todas tem como finalidade distinguir células do tecido conjuntivo circundante. Muitas da formulações produzem cor verde na queratina e fibras musculares, azul ou verde em colágeno e tecido ósseo, vermelho brilhante ou rosa no citoplasma, e castanho escuro a preto nos núcleos das células. Uma variante comum é o corante tricromo de Lillie. Este é frequentemente chamado tricromo de Masson. Difere nos corantes usados, suas concentrações, e os tempos de imersão. (pt) 马森三色染色法（Masson's trichrome stain），是一种用于组织学的染色方法。从（1880–1959年）的原始配方开发而来的新配方具有不同的特定应用，但都适用于把细胞从周围的结缔组织区分开。 大多数的配方将角蛋白以及肌肉纖維染为红色，将胶原蛋白与骨质染为蓝色或绿色，将胞质染为淡红色或是粉红色，并将细胞核染为深棕色或黑色。 (zh) |
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| rdfs:comment | Die Masson-Trichrom-Färbung ist eine histologische Trichrom-Färbungsmethode. (de) La tinción tricrómica de Masson, al igual que otras tinciones tricrómicas, es una técnica de coloración especial que permite visualizar claramente las fibras de colágeno tipo I que forman fibras gruesas o haces, diseñados para dar resistencia; también evidencia, aunque en menor intensidad, las . Se emplean tres colorantes para diferenciar el núcleo celular, el citoplasma y las fibras de colágeno. (es) 马森三色染色法（Masson's trichrome stain），是一种用于组织学的染色方法。从（1880–1959年）的原始配方开发而来的新配方具有不同的特定应用，但都适用于把细胞从周围的结缔组织区分开。 大多数的配方将角蛋白以及肌肉纖維染为红色，将胶原蛋白与骨质染为蓝色或绿色，将胞质染为淡红色或是粉红色，并将细胞核染为深棕色或黑色。 (zh) Masson's trichrome is a three-colour staining procedure used in histology. The recipes evolved from Claude L. Pierre Masson's (1880–1959) original formulation have different specific applications, but all are suited for distinguishing cells from surrounding connective tissue. Most recipes produce red keratin and muscle fibers, blue or green collagen and bone, light red or pink cytoplasm, and dark brown to black cell nuclei. The trichrome is applied by immersion of the fixated sample into Weigert's iron hematoxylin, and then three different solutions, labeled A, B, and C: (en) Corante ou coloração tricromo ou tricrômica de Masson é uma combinação de corantes usados em um protocolo de coloração de três cores em histologia. As formulações desta coloração envolvem a formulação original de Masson para diferentes aplicações, mas todas tem como finalidade distinguir células do tecido conjuntivo circundante. Muitas da formulações produzem cor verde na queratina e fibras musculares, azul ou verde em colágeno e tecido ósseo, vermelho brilhante ou rosa no citoplasma, e castanho escuro a preto nos núcleos das células. (pt) |
| rdfs:label | Masson-Trichrom-Färbung (de) Tinción tricrómica de Masson (es) Masson's trichrome stain (en) Corante tricromo de Masson (pt) Masson三色染色法 (zh) |
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