Niacin test (original) (raw)
The niacin test has been widely used since the 1960s to identify mycobacteria at the species level in the clinical laboratory. The niacin test detects niacin (nicotinic acid) in aqueous extracts of a culture. M. tuberculosis strains that test negative for the niacin test are very rare. Redox reactions happening in Mycobacterium species produce niacin as a part of energy metabolism. Even though all mycobacteria produce niacin, M. tuberculosis accumulates an excess of niacin because of its inability to process niacin, excreting the excess niacin into the culture media, thus allowing it to be detected using the niacin test. The niacin test is typically only conducted on slow-growing, granular, tan colored colonies, as these are the morphology characteristics of M. tuberculosis on an agar plat
| Property | Value |
|---|---|
| dbo:abstract | The niacin test has been widely used since the 1960s to identify mycobacteria at the species level in the clinical laboratory. The niacin test detects niacin (nicotinic acid) in aqueous extracts of a culture. M. tuberculosis strains that test negative for the niacin test are very rare. Redox reactions happening in Mycobacterium species produce niacin as a part of energy metabolism. Even though all mycobacteria produce niacin, M. tuberculosis accumulates an excess of niacin because of its inability to process niacin, excreting the excess niacin into the culture media, thus allowing it to be detected using the niacin test. The niacin test is typically only conducted on slow-growing, granular, tan colored colonies, as these are the morphology characteristics of M. tuberculosis on an agar plate. Because of its affordability compared to expensive identification methods like pyrosequencing or MALDI-TOF MS that require expensive machines and reagents. (en) |
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| dbo:wikiPageWikiLink | dbr:Potassium_thiocyanate dbr:Pyridine dbr:Aqueous_solution dbc:Bacteriology dbr:Pyrosequencing dbr:Citric_acid dbr:Glutaconaldehyde dbr:Morphology_(biology) dbr:Aromatic_amine dbc:Mycobacteria dbr:Sterilization_(microbiology) dbr:Matrix-assisted_laser_desorption/ionization dbr:4-Aminosalicylic_acid dbr:Cyanogen_chloride dbr:Nicotinamide_adenine_dinucleotide dbr:Nicotinic_acid dbr:PH dbr:Redox dbr:Acid-fastness dbr:Agar_plate dbr:Chloramine-T dbr:Biosafety_cabinet dbr:Biosafety_level dbr:Metabolism dbr:Niacin_(substance) dbr:Reagent dbr:Mycobacterium_canetti dbr:Mycobacterium_africanum dbr:Mycobacterium_tuberculosis dbr:Mycobacterium_tuberculosis_complex dbr:Clinical_laboratory dbr:Mycobacteria dbr:Lab_tech dbr:File:Mycobacterium_smegmatis.jpg dbr:File:Niacin_Test.png |
| dbp:wikiPageUsesTemplate | dbt:Reflist dbt:Short_description |
| dct:subject | dbc:Bacteriology dbc:Mycobacteria |
| rdfs:comment | The niacin test has been widely used since the 1960s to identify mycobacteria at the species level in the clinical laboratory. The niacin test detects niacin (nicotinic acid) in aqueous extracts of a culture. M. tuberculosis strains that test negative for the niacin test are very rare. Redox reactions happening in Mycobacterium species produce niacin as a part of energy metabolism. Even though all mycobacteria produce niacin, M. tuberculosis accumulates an excess of niacin because of its inability to process niacin, excreting the excess niacin into the culture media, thus allowing it to be detected using the niacin test. The niacin test is typically only conducted on slow-growing, granular, tan colored colonies, as these are the morphology characteristics of M. tuberculosis on an agar plat (en) |
| rdfs:label | Niacin test (en) |
| owl:sameAs | wikidata:Niacin test https://global.dbpedia.org/id/2eAxa |
| prov:wasDerivedFrom | wikipedia-en:Niacin_test?oldid=1113946517&ns=0 |
| foaf:depiction | wiki-commons:Special:FilePath/Mycobacterium_smegmatis.jpg wiki-commons:Special:FilePath/Niacin_Test.png |
| foaf:isPrimaryTopicOf | wikipedia-en:Niacin_test |
| is rdfs:seeAlso of | dbr:Phenotypic_testing_of_mycobacteria |
| is foaf:primaryTopic of | wikipedia-en:Niacin_test |
