Nile red (original) (raw)
Nilrot, auch als Nilblau-Oxazon bezeichnet, ist ein lipophiler fluoreszierender Phenoxazin-Farbstoff.
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| dbo:abstract | Nilrot, auch als Nilblau-Oxazon bezeichnet, ist ein lipophiler fluoreszierender Phenoxazin-Farbstoff. (de) Nile red (also known as Nile blue oxazone) is a lipophilic stain. Nile red stains intracellular lipid droplets yellow. In most polar solvents, Nile red will not fluoresce; however, when in a lipid-rich environment, it can be intensely fluorescent, with varying colors from deep red (for polar membrane lipid) to strong yellow-gold emission (for neutral lipid in intracellular storages). The dye is highly solvatochromic and its emission and excitation wavelength both shift depending on solvent polarity and in polar media will hardly fluoresce at all. Nile red has applications in cell biology, where it can be used as a membrane dye which can be readily visualized using an epifluorescence microscope with excitation and emission wavelengths usually shared with red fluorescent protein. Nile red has also been used as part of a sensitive detection process for microplastics in bottled water. Additionally, nile red is a remarkable candidator in fabricating membrane for different sensors to detect environmental changes, such as taste, gas, pH, etc. In triglycerides (a neutral lipid), Nile red has an excitation maximum of about 515 nm (green), and emission maximum of about 585 nm (yellow-orange). In contrast, in phospholipids (polar lipids), Nile red has an excitation maximum of about 554 nm (green), and an emission maximum of about 638 nm (red). (en) Czerwień Nilu – organiczny związek chemiczny, barwnik lipofilowy o zdolności do fluorescencji. Zarówno maksymalna emisja, jak i są silnie zależne od użytego rozpuszczalnika. Wyższa polarność rozpuszczalnika na ogół prowadzi do przesunięcia maksimum fluorescencji ku czerwieni i zmniejszenia wydajności kwantowej oraz czasu życia fluorescencji. (pl) |
| dbo:alternativeName | Nile red, Nile blue oxazone (en) |
| dbo:iupacName | 9-(Diethylamino)-5H-benzo[a]phenoxazin-5-one (en) |
| dbo:thumbnail | wiki-commons:Special:FilePath/Nilrot.svg?width=300 |
| dbo:wikiPageID | 1574353 (xsd:integer) |
| dbo:wikiPageLength | 7462 (xsd:nonNegativeInteger) |
| dbo:wikiPageRevisionID | 1120596095 (xsd:integer) |
| dbo:wikiPageWikiLink | dbr:Bottled_water dbr:Structural_analog dbr:MTBE dbr:Condensation_reaction dbr:Lipophilic dbr:Microplastics dbr:2-Naphthol dbr:Cell_(biology) dbr:Toluene dbr:Lipid dbr:Acetone dbr:Acetonitrile dbr:Cyclohexane dbr:Ethanol dbr:Ethyl_acetate dbc:Oxazine_dyes dbr:Nile_blue dbr:Red_fluorescent_protein dbc:Diethylamino_compounds dbr:Hexane dbr:Bacillus_subtilis dbc:Vital_stains dbr:Sulfuric_acid dbr:Dichloromethane dbr:Dimethylformamide dbr:Methanol dbr:Water dbr:Iminium dbr:Solvatochromism dbr:Epifluorescence_microscope dbr:File:Nile_Red_synthesis.png |
| dbp:imagefile | Nile-red-3D-balls.png (en) Nilrot.svg (en) |
| dbp:imagesize | 200 (xsd:integer) 300 (xsd:integer) |
| dbp:othernames | Nile red, Nile blue oxazone (en) |
| dbp:pin | 9 (xsd:integer) |
| dbp:verifiedfields | changed (en) |
| dbp:verifiedrevid | 476992673 (xsd:integer) |
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| dcterms:subject | dbc:Oxazine_dyes dbc:Diethylamino_compounds dbc:Vital_stains |
| rdf:type | owl:Thing dul:ChemicalObject dbo:ChemicalSubstance wikidata:Q11173 yago:WikicatNaphthalenes yago:WikicatVitalStains yago:WikicatOxazinDyes yago:AbsorptionIndicator114598383 yago:Abstraction100002137 yago:Appearance104673965 yago:Attribute100024264 yago:Chemical114806838 yago:ColoringMaterial114984973 yago:Compound114818238 yago:Dye114985383 yago:Fluorescein114987695 yago:Hydrocarbon114911057 yago:Indicator114917208 yago:Ketone114926294 yago:Material114580897 yago:Matter100020827 yago:Naphthalene114958564 yago:OrganicCompound114727670 yago:Part113809207 yago:PhysicalEntity100001930 yago:Quality104723816 yago:Relation100031921 yago:WikicatKetones dbo:ChemicalCompound yago:Stain104695176 yago:Substance100019613 yago:WikicatFluorescentDyes umbel-rc:ChemicalSubstanceType |
| rdfs:comment | Nilrot, auch als Nilblau-Oxazon bezeichnet, ist ein lipophiler fluoreszierender Phenoxazin-Farbstoff. (de) Czerwień Nilu – organiczny związek chemiczny, barwnik lipofilowy o zdolności do fluorescencji. Zarówno maksymalna emisja, jak i są silnie zależne od użytego rozpuszczalnika. Wyższa polarność rozpuszczalnika na ogół prowadzi do przesunięcia maksimum fluorescencji ku czerwieni i zmniejszenia wydajności kwantowej oraz czasu życia fluorescencji. (pl) Nile red (also known as Nile blue oxazone) is a lipophilic stain. Nile red stains intracellular lipid droplets yellow. In most polar solvents, Nile red will not fluoresce; however, when in a lipid-rich environment, it can be intensely fluorescent, with varying colors from deep red (for polar membrane lipid) to strong yellow-gold emission (for neutral lipid in intracellular storages). The dye is highly solvatochromic and its emission and excitation wavelength both shift depending on solvent polarity and in polar media will hardly fluoresce at all. (en) |
| rdfs:label | Nilrot (de) Nile red (en) Czerwień Nilu (pl) |
| owl:sameAs | freebase:Nile red yago-res:Nile red wikidata:Nile red http://azb.dbpedia.org/resource/نیل_قرمز http://bs.dbpedia.org/resource/Kongo_crvena dbpedia-de:Nile red dbpedia-pl:Nile red dbpedia-sh:Nile red https://global.dbpedia.org/id/54FJc |
| prov:wasDerivedFrom | wikipedia-en:Nile_red?oldid=1120596095&ns=0 |
| foaf:depiction | wiki-commons:Special:FilePath/Nile_red_01.jpg wiki-commons:Special:FilePath/Bacillus_subtilis_stained_with_Nile_Red.jpg wiki-commons:Special:FilePath/Nile-red-3D-balls.png wiki-commons:Special:FilePath/Nile_Red_synthesis.png wiki-commons:Special:FilePath/Nilrot.svg |
| foaf:isPrimaryTopicOf | wikipedia-en:Nile_red |
| is dbo:wikiPageRedirects of | dbr:Nile_blue_oxazone |
| is dbo:wikiPageWikiLink of | dbr:List_of_dyes dbr:Dye dbr:Kreft's_dichromaticity_index dbr:Staining dbr:Lipid-laden_alveolar_macrophage dbr:Nile_blue dbr:Fluorophore dbr:Phenoxazine dbr:Solvent dbr:Oxazines dbr:Super-resolution_microscopy dbr:Peptoid_nanosheet dbr:Nile_blue_oxazone |
| is foaf:primaryTopic of | wikipedia-en:Nile_red |
