Physical mapping (original) (raw)
Physical map is a technique used in molecular biology to find the order and physical distance between DNA base pairs by DNA markers. It is one of the gene mapping techniques which can determine the sequence of DNA base pairs with high accuracy. Genetic mapping, another approach of gene mapping, can provide markers needed for the physical mapping. However, as the former deduces the relative gene position by recombination frequencies, it is less accurate than the latter.
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| dbo:abstract | Physical map is a technique used in molecular biology to find the order and physical distance between DNA base pairs by DNA markers. It is one of the gene mapping techniques which can determine the sequence of DNA base pairs with high accuracy. Genetic mapping, another approach of gene mapping, can provide markers needed for the physical mapping. However, as the former deduces the relative gene position by recombination frequencies, it is less accurate than the latter. Physical mapping uses DNA fragments and DNA markers to assemble larger DNA pieces. With the overlapping regions of the fragments, researchers can deduce the positions of the DNA bases. There are different techniques to visualize the gene location, including somatic cell hybridization, radiation hybridization and in situ hybridization. The different approaches to physical mapping are available for analyzing different sizes of genome and achieving different levels of accuracy. Low- and high-resolution mapping are two classes for various resolution of genome, particularly for the investigation of chromosomes. The three basic varieties of physical mapping are fluorescent in situ hybridization (FISH), restriction site mapping and sequencing by clones. The goal of physical mapping, as a common mechanism under genomic analysis, is to obtain a complete genome sequence in order to deduce any association between the target DNA sequence and phenotypic traits. If the actual positions of genes which control certain phenotypes are known, it is possible to resolve genetic diseases by providing advice on prevention and developing new treatments. (en) |
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| dbo:wikiPageWikiLink | dbr:Electrophoresis dbr:Nucleotide dbr:Metaphase dbr:DNA_methylation dbr:Interphase dbr:Polymerase_chain_reaction dbr:Gene_expression dbr:Gene_mapping dbr:Genetic_marker dbr:Genomic_library dbr:Chromosomes dbr:Enzyme dbr:Contigs dbr:Coprecipitation dbr:Calcium_phosphate dbr:Microplate dbr:Transcription_(biology) dbr:Synteny dbc:Molecular_biology_techniques dbr:Exponential_function dbr:Base_pair dbr:Chromatin dbr:Chromosome dbr:Fluorescence_in_situ_hybridization dbr:Restriction_enzyme dbr:Hybridization_probe dbr:Phenotypic_trait dbr:Translation_(biology) dbr:Phenotype dbr:Somatic_fusion dbr:Human_Genome_Project dbr:Organ_(anatomy) dbr:Immortalised_cell_line dbr:In_situ dbr:In_situ_hybridization dbr:Restriction_site dbr:Radiation_hybrid_mapping dbr:Yeast_artificial_chromosomes dbr:Molecular_biology dbr:Prometaphase dbr:Somatic_cell_hybridisation dbr:Radiation_hybrid dbr:Recombination_Frequency dbr:Genetic_mapping |
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| rdfs:comment | Physical map is a technique used in molecular biology to find the order and physical distance between DNA base pairs by DNA markers. It is one of the gene mapping techniques which can determine the sequence of DNA base pairs with high accuracy. Genetic mapping, another approach of gene mapping, can provide markers needed for the physical mapping. However, as the former deduces the relative gene position by recombination frequencies, it is less accurate than the latter. (en) |
| rdfs:label | Physical mapping (en) |
| owl:sameAs | wikidata:Physical mapping https://global.dbpedia.org/id/9i4Bt |
| prov:wasDerivedFrom | wikipedia-en:Physical_mapping?oldid=1103811768&ns=0 |
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