Protein L (original) (raw)
Das Protein L ist ein Protein aus , das Antikörper bindet.
| Property | Value |
|---|---|
| dbo:abstract | Das Protein L ist ein Protein aus , das Antikörper bindet. (de) Protein L was first isolated from the surface of bacterial species Peptostreptococcus magnus and was found to bind immunoglobulins through L chain interaction, from which the name was suggested. It consists of 719 amino acid residues. The molecular weight of Protein L purified from the cell walls of Peptostreptoccus magnus was first estimated as 95kD by SDS-PAGE in the presence of reducing agent 2-mercaptoethanol, while the molecular weight was determined to 76kD by gel chromotography in the presence of 6 M guanidine HCl. Protein L does not contain any interchain disulfide loops, nor does it consist of disulfide-linked subunits. It is an acidic molecule with a pI of 4.0. Unlike Protein A and Protein G, which bind to the Fc region of immunoglobulins (antibodies), Protein L binds antibodies through light chain interactions. Since no part of the heavy chain is involved in the binding interaction, Protein L binds a wider range of antibody classes than Protein A or G. Protein L binds to representatives of all antibody classes, including IgG, IgM, IgA, IgE and IgD. Single chain variable fragments (scFv) and Fab fragments also bind to Protein L. Despite this wide binding range, Protein L is not a universal antibody-binding protein. Protein L binding is restricted to those antibodies that contain kappa light chains. In humans and mice, most antibody molecules contain kappa (κ) light chains and the remainder have lambda (λ) light chains. Protein L is only effective in binding certain subtypes of kappa light chains. For example, it binds human VκI, VκIII and VκIV subtypes but does not bind the VκII subtype. Binding of mouse immunoglobulins is restricted to those having VκI light chains. Given these specific requirements for effective binding, the main application for immobilized Protein L is purification of monoclonal antibodies from ascites or cell culture supernatant that are known to have the kappa light chain. Protein L is extremely useful for purification of VLκ-containing monoclonal antibodies from culture supernatant because it does not bind bovine immunoglobulins, which are often present in the media as a serum supplement. Also, Protein L does not interfere with the antigen-binding site of the antibody, making it useful for immunoprecipitation assays, even using IgM. (en) |
| dbo:symbol | PpL |
| dbo:thumbnail | wiki-commons:Special:FilePath/Protein_L_1MHH.png?width=300 |
| dbo:wikiPageID | 10919113 (xsd:integer) |
| dbo:wikiPageLength | 5917 (xsd:nonNegativeInteger) |
| dbo:wikiPageRevisionID | 1085761900 (xsd:integer) |
| dbo:wikiPageWikiLink | dbr:Protein_A dbr:Bovine dbr:Protein_A/G dbr:Protein_G dbc:Immunology dbr:Monoclonal_antibodies dbc:Proteins dbr:Cell_culture dbr:Isoelectric_point dbr:Protein dbr:Ascites dbr:IgG dbr:IgM dbr:IgE dbr:Antibodies dbr:IgA dbr:Immunoglobulin_light_chain dbr:Immunoprecipitation dbr:Supernatant dbr:ScFv dbr:Blood_serum dbr:Peptostreptococcus_magnus dbr:IgD |
| dbp:caption | Structure of protein L binding to the light chain of a murine Fab . (en) |
| dbp:interpro | IPR003147 (en) |
| dbp:name | Protein L b1 domain (en) |
| dbp:pdb | , , , , , , , , , , , , , , , , , (en) |
| dbp:pfam | PF02246 (en) |
| dbp:scop | 1 (xsd:integer) |
| dbp:symbol | PpL (en) |
| dbp:wikiPageUsesTemplate | dbt:Reflist dbt:PDB2 dbt:PDB dbt:Pfam_box |
| dcterms:subject | dbc:Immunology dbc:Proteins |
| rdf:type | owl:Thing dbo:Biomolecule wikidata:Q206229 wikidata:Q8054 yago:Abstraction100002137 yago:Chemical114806838 yago:Compound114818238 yago:Macromolecule114944888 yago:Material114580897 yago:Matter100020827 yago:Molecule114682133 yago:OrganicCompound114727670 yago:Part113809207 yago:PhysicalEntity100001930 yago:Protein114728724 yago:Relation100031921 dbo:Protein yago:Substance100019613 yago:Thing100002452 yago:Unit109465459 yago:WikicatProteins |
| rdfs:comment | Das Protein L ist ein Protein aus , das Antikörper bindet. (de) Protein L was first isolated from the surface of bacterial species Peptostreptococcus magnus and was found to bind immunoglobulins through L chain interaction, from which the name was suggested. It consists of 719 amino acid residues. The molecular weight of Protein L purified from the cell walls of Peptostreptoccus magnus was first estimated as 95kD by SDS-PAGE in the presence of reducing agent 2-mercaptoethanol, while the molecular weight was determined to 76kD by gel chromotography in the presence of 6 M guanidine HCl. Protein L does not contain any interchain disulfide loops, nor does it consist of disulfide-linked subunits. It is an acidic molecule with a pI of 4.0. Unlike Protein A and Protein G, which bind to the Fc region of immunoglobulins (antibodies), Protein L binds antibodies (en) |
| rdfs:label | Protein L (de) Protein L (en) |
| owl:sameAs | freebase:Protein L wikidata:Protein L dbpedia-de:Protein L https://global.dbpedia.org/id/4uA8t yago-res:Protein L |
| prov:wasDerivedFrom | wikipedia-en:Protein_L?oldid=1085761900&ns=0 |
| foaf:depiction | wiki-commons:Special:FilePath/Protein_L_1MHH.png |
| foaf:isPrimaryTopicOf | wikipedia-en:Protein_L |
| is dbo:wikiPageWikiLink of | dbr:Protein_A dbr:Protein_M dbr:Antigen dbr:Index_of_protein-related_articles dbr:Protein_A/G dbr:Protein_G dbr:Folding@home dbr:B1_domain dbr:Immunoglobulin-binding_protein dbr:Single-chain_variable_fragment |
| is foaf:primaryTopic of | wikipedia-en:Protein_L |
