| dbo:abstract |
T4-DNA-Polymerase ist ein Enzym aus der Gruppe der DNA-Polymerasen, das vom Gen 43 des Bakteriophagen T4 codiert wird. (de) T7 DNA polymerase is an enzyme used during the DNA replication of the T7 bacteriophage. During this process, the DNA polymerase “reads” existing DNA strands and creates two new strands that match the existing ones. The T7 DNA polymerase requires a host factor, E. coli thioredoxin, in order to carry out its function. This helps stabilize the binding of the necessary protein to the primer-template to improve processivity by more than 100-fold, which is a feature unique to this enzyme. It is a member of the Family A DNA polymerases, which include E. coli DNA polymerase I and Taq DNA polymerase. This polymerase has various applications in site-directed mutagenesis as well as a high-fidelity enzyme suitable for PCR. It has also served as the precursor to Sequenase, an engineered-enzyme optimized for DNA sequencing. (en) |
| dbo:thumbnail |
wiki-commons:Special:FilePath/1T7P_Crystal_Structure.jpg?width=300 |
| dbo:wikiPageExternalLink |
http://www.rcsb.org/pdb/explore/explore.do%3FstructureId=1T7P |
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19309200 (xsd:integer) |
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| dbo:wikiPageWikiLink |
dbr:Primer_(molecular_biology) dbr:Pyrophosphate dbr:Nucleophilic_addition dbr:Nucleotide dbr:Processivity dbr:Base_(chemistry) dbc:Phage_proteins dbr:Hydrogen_bond dbr:DNA dbr:DNA_polymerase dbr:DNA_polymerase_I dbr:DNA_replication dbr:Carbonyl dbr:Polymerase_chain_reaction dbr:Nucleoside dbc:EC_2.7.7 dbr:Gene_product dbr:Nucleophile dbr:Entropy dbr:Enzyme dbr:Gene dbr:Polyphosphate dbr:Ligands dbr:Lysine dbr:Taq_polymerase dbr:Thioredoxin dbr:Backbone_chain dbr:Active_site dbr:Tyrosine dbr:Helicase dbr:Acid_dissociation_constant dbr:Alanine dbr:Amino_acid dbr:DNA_clamp dbr:Escherichia_coli dbr:Rate-determining_step dbr:Histidine dbc:DNA_replication dbr:Hydrophobic_effect dbr:Primase dbr:Arginine dbr:Aspartic_acid dbr:T7_phage dbr:C-terminus dbr:DNA_primer dbr:DNA_sequence dbr:Catalytic_cycle dbr:Hydroxyl dbr:Exonuclease dbr:Dipolar_bond dbr:Phosphodiester_bond dbr:Molecular_biology dbr:Ribonucleotide dbr:Scissile_bond dbr:Replisome dbr:Sanger_sequencing dbr:Site-directed_mutagenesis dbr:Single-stranded_DNA_binding_protein dbr:Dideoxynucleotides dbr:File:Figure_3_modified.jpg dbr:File:General_DNA_polymerase_mechanism.tif dbr:File:Phage_T7_replication_machinery.png |
| dbp:caption |
Figure 1. Crystal structure of T7 DNA replication complex. Rendered from PDB 1T7P. (en) |
| dbp:casNumber |
9012 (xsd:integer) |
| dbp:ecnumber |
2.700000 (xsd:double) |
| dbp:name |
DNA-directed DNA polymerase (en) Nucleotide kinase (en) Single-stranded DNA-binding protein (en) |
| dbp:organism |
Enterobacteria phage T7 (en) |
| dbp:symbol |
1.700000 (xsd:double) 2.500000 (xsd:double) 5 (xsd:integer) |
| dbp:uniprot |
P00581 (en) P03696 (en) P03781 (en) |
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dbt:Infobox_nonhuman_protein dbt:Kinases dbt:Main dbt:Portal_bar dbt:Reflist dbt:DNA_replication dbt:PCR |
| dcterms:subject |
dbc:Phage_proteins dbc:EC_2.7.7 dbc:DNA_replication |
| gold:hypernym |
dbr:Enzyme |
| rdf:type |
dbo:Protein |
| rdfs:comment |
T4-DNA-Polymerase ist ein Enzym aus der Gruppe der DNA-Polymerasen, das vom Gen 43 des Bakteriophagen T4 codiert wird. (de) T7 DNA polymerase is an enzyme used during the DNA replication of the T7 bacteriophage. During this process, the DNA polymerase “reads” existing DNA strands and creates two new strands that match the existing ones. The T7 DNA polymerase requires a host factor, E. coli thioredoxin, in order to carry out its function. This helps stabilize the binding of the necessary protein to the primer-template to improve processivity by more than 100-fold, which is a feature unique to this enzyme. It is a member of the Family A DNA polymerases, which include E. coli DNA polymerase I and Taq DNA polymerase. (en) |
| rdfs:label |
T4-DNA-Polymerase (de) T7 DNA polymerase (en) |
| owl:sameAs |
freebase:T7 DNA polymerase wikidata:T7 DNA polymerase dbpedia-de:T7 DNA polymerase https://global.dbpedia.org/id/4vtXY |
| prov:wasDerivedFrom |
wikipedia-en:T7_DNA_polymerase?oldid=1067003427&ns=0 |
| foaf:depiction |
wiki-commons:Special:FilePath/Phage_T7_replication_machinery.png wiki-commons:Special:FilePath/1T7P_Crystal_Structure.jpg wiki-commons:Special:FilePath/Figure_3_modified.jpg |
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wikipedia-en:T7_DNA_polymerase |
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dbr:Protein_moonlighting dbr:Taq_polymerase dbr:Hachimoji_DNA dbr:Charles_C._Richardson dbr:Variants_of_PCR dbr:T7_DNA_helicase dbr:T7_RNA_polymerase |
| is foaf:primaryTopic of |
wikipedia-en:T7_DNA_polymerase |
