Tetramer assay (original) (raw)
A tetramer assay (also known as a tetramer stain) is a procedure that uses tetrameric proteins to detect and quantify T cells that are specific for a given antigen within a blood sample. The tetramers used in the assay are made up of four major histocompatibility complex (MHC) molecules, which are found on the surface of most cells in the body. MHC molecules present peptides to T-cells as a way to communicate the presence of viruses, bacteria, cancerous mutations, or other antigens in a cell. If a T-cell's receptor matches the peptide being presented by an MHC molecule, an immune response is triggered. Thus, MHC tetramers that are bioengineered to present a specific peptide can be used to find T-cells with receptors that match that peptide.The tetramers are labeled with a fluorophore, allo
,_and_an_MHC_molecule_on_the_surface_of_an_antigen_presenting_cell_binding_to_T-cell_receptors_(right).png?width=300)
| Property | Value |
|---|---|
| dbo:abstract | A tetramer assay (also known as a tetramer stain) is a procedure that uses tetrameric proteins to detect and quantify T cells that are specific for a given antigen within a blood sample. The tetramers used in the assay are made up of four major histocompatibility complex (MHC) molecules, which are found on the surface of most cells in the body. MHC molecules present peptides to T-cells as a way to communicate the presence of viruses, bacteria, cancerous mutations, or other antigens in a cell. If a T-cell's receptor matches the peptide being presented by an MHC molecule, an immune response is triggered. Thus, MHC tetramers that are bioengineered to present a specific peptide can be used to find T-cells with receptors that match that peptide.The tetramers are labeled with a fluorophore, allowing tetramer-bound T-cells to be analyzed with flow cytometry. Quantification and sorting of T-cells by flow cytometry enables researchers to investigate immune response to viral infection and vaccine administration as well as functionality of antigen-specific T-cells. Generally, if a person's immune system has encountered a pathogen, the individual will possess T cells with specificity toward some peptide on that pathogen. Hence, if a tetramer stain specific for a pathogenic peptide results in a positive signal, this may indicate that the person's immune system has encountered and built a response to that pathogen. (en) |
| dbo:thumbnail | wiki-commons:Special:FilePath/An_MHC_tetramer_bindi...-cell_receptors_(right).png?width=300 |
| dbo:wikiPageID | 10290414 (xsd:integer) |
| dbo:wikiPageLength | 12305 (xsd:nonNegativeInteger) |
| dbo:wikiPageRevisionID | 1057768863 (xsd:integer) |
| dbo:wikiPageWikiLink | dbr:Natural_killer_T_cell dbr:Monomer dbr:Biotin dbc:Flow_cytometry dbr:Antigen dbr:Antigen-presenting_cell dbr:Beta-2_microglobulin dbr:Peptide dbr:Cytokine dbr:Cytotoxic_T_cell dbr:Vaccine dbr:Major_histocompatibility_complex dbr:Pathogen dbr:T_helper_cell dbr:HIV dbr:Lipid dbr:Escherichia_coli dbr:Flow_cytometry dbr:Fluorophore dbr:Glycolipid dbr:MHC_class_II dbr:RNA dbc:Blood_tests dbr:T_cells dbr:CD1 dbr:CD4 dbr:CD80 dbr:Immunology dbr:Macrophage dbr:Immune_system dbr:Tetramer dbr:File:An_MHC_tetramer_binding_to_T-cell...nding_to_T-cell_receptors_(right).png |
| dbp:name | Tetramer assay (en) |
| dbp:purpose | uses tetrameric proteins to detect and quantify T cells (en) |
| dbp:synonyms | Tetramer stain (en) |
| dbp:wikiPageUsesTemplate | dbt:Citation_needed dbt:Cite_journal dbt:Infobox_diagnostic dbt:Refbegin dbt:Refend dbt:Reflist |
| dcterms:subject | dbc:Flow_cytometry dbc:Blood_tests |
| gold:hypernym | dbr:Procedure |
| rdf:type | yago:WikicatBloodTests dbo:AnatomicalStructure yago:Abstraction100002137 yago:Act100030358 yago:Activity100407535 yago:Assay100791527 yago:Attempt100786195 yago:Biopsy105740929 yago:BloodTest105742551 yago:DiagnosticTest105739043 yago:Event100029378 yago:PsychologicalFeature100023100 yago:YagoPermanentlyLocatedEntity yago:Test100791078 |
| rdfs:comment | A tetramer assay (also known as a tetramer stain) is a procedure that uses tetrameric proteins to detect and quantify T cells that are specific for a given antigen within a blood sample. The tetramers used in the assay are made up of four major histocompatibility complex (MHC) molecules, which are found on the surface of most cells in the body. MHC molecules present peptides to T-cells as a way to communicate the presence of viruses, bacteria, cancerous mutations, or other antigens in a cell. If a T-cell's receptor matches the peptide being presented by an MHC molecule, an immune response is triggered. Thus, MHC tetramers that are bioengineered to present a specific peptide can be used to find T-cells with receptors that match that peptide.The tetramers are labeled with a fluorophore, allo (en) |
| rdfs:label | Tetramer assay (en) |
| owl:sameAs | freebase:Tetramer assay yago-res:Tetramer assay wikidata:Tetramer assay https://global.dbpedia.org/id/4vZgT |
| prov:wasDerivedFrom | wikipedia-en:Tetramer_assay?oldid=1057768863&ns=0 |
| foaf:depiction | wiki-commons:Special:FilePath/An_MHC_tetramer_bindi...nding_to_T-cell_receptors_(right).png |
| foaf:isPrimaryTopicOf | wikipedia-en:Tetramer_assay |
| is dbo:wikiPageWikiLink of | dbr:Streptavidin dbr:Assay dbr:Tetrameric_protein |
| is foaf:primaryTopic of | wikipedia-en:Tetramer_assay |
