Isolation and comparison of mesenchymal stem-like cells from human gastric cancer and adjacent non-cancerous tissues (original) (raw)

Abstract

Purpose

Mesenchymal stem cell (MSC)-like cells, a subpopulation of tumor microenvironment, have been isolated from several tumor tissues. In our previous study, we identified MSC-like cells in human gastric cancer tissues and found their characteristics to be similar to those of bone marrow MSCs. However, whether there are MSC-like cells in adjacent non-cancerous tissues and any difference between the MSC-like cells derived from tumor and non-cancerous tissues are not clear. The aim of this study is to study and research the differences of two mesenchymal stem-like cells.

Methods

We demonstrate that MSC-like cells can be isolated from both human gastric tumor (hGC-MSCs) and adjacent non-cancerous tissues (hGCN-MSCs) of the same patient. We further compared the characteristics between hGC-MSCs and hGCN-MSCs.

Results

Our results revealed that hGC-MSCs and hGCN-MSCs possessed similar morphological properties; stem cell-associated gene expression, as well as multipotential differentiation capability. We also found differences in cell surface markers, pluripotency, and proliferation-related gene expression between hGCN-MSCs and hGC-MSCs.

Conclusions

Our findings suggest that MSC-like cells are components of the tumor microenvironment and provide proof for the origin of carcinoma-associated fibroblast, therefore may potentially be used as a target for gastric cancer diagnosis and therapy.

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Acknowledgments

We thank Dr. Ruiwen Zhang (Comprehensive Cancer Center and Gene Therapy Center, University of Alabama, School of Medicine, Birmingham, USA) for assistance in editing this manuscript. This work was supported by the National Natural Science Foundation of China (grant no. 30471983), the Natural Science Foundation of the Ministry of Public Health of China (grant no. WKJ2005-2-024), Jiangsu Province’s Outstanding Medical Academic Leader Program (LJ200614), and the Natural Science Foundation of the Ministry of Education of the Jiangsu Province (grant no. 06KJD310040).

Conflict of Interest

Neither the submitted paper (Isolation and comparison of mesenchymal stem-like cells from human gastric cancer and adjacent non-cancerous tissues) nor any similar paper, either in whole or in part, has been or will be submitted to or published in any other primary scientific journal. All of the authors are aware of and agree to the content of the paper and their being listed as author on the paper. There are no financial or other interests with regard to the paper that represent a conflict of interest.

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Authors and Affiliations

1. School of Medical Science and Laboratory Medicine, Jiangsu University, 301 Xuefu Road, Zhenjiang, 212013, Jiangsu, People’s Republic of China
   Xiaomeng Xu, Xu Zhang, Hui Qian, Wei Zhu, Huiling Cao, Mei Wang, Yuan Chen & Wenrong Xu
2. Department of Clinical Laboratory Medicine, The Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu, People’s Republic of China
   Wenrong Xu
3. Department of Surgery, The First People’s Hospital of Zhenjiang, 8 Dianli Road, Zhenjiang, 212013, Jiangsu, People’s Republic of China
   Sheng Wang

Authors

1. Xiaomeng Xu
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2. Xu Zhang
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3. Sheng Wang
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4. Hui Qian
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5. Wei Zhu
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6. Huiling Cao
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7. Mei Wang
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8. Yuan Chen
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9. Wenrong Xu
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Correspondence toWenrong Xu.

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Xu, X., Zhang, X., Wang, S. et al. Isolation and comparison of mesenchymal stem-like cells from human gastric cancer and adjacent non-cancerous tissues.J Cancer Res Clin Oncol 137, 495–504 (2011). https://doi.org/10.1007/s00432-010-0908-6

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• Received: 07 March 2010
• Accepted: 27 April 2010
• Published: 15 May 2010
• Issue Date: March 2011
• DOI: https://doi.org/10.1007/s00432-010-0908-6

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