Histone3 lysine4 trimethylation regulated by the facilitates chromatin transcription complex is critical for DNA cleavage in class switch recombination. (original) (raw)
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| タイトル: | Histone3 lysine4 trimethylation regulated by the facilitates chromatin transcription complex is critical for DNA cleavage in class switch recombination. |
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| 著者: | Stanlie, AndreAida, MasatoshiMuramatsu, MasamichiHonjo, Tasuku   Begum, Nasim A |
| 著者名の別形: | 相田, 将俊本庶, 佑 |
| 発行日: | Dec-2010 |
| 出版者: | National Academy of Sciences |
| 誌名: | Proceedings of the National Academy of Sciences (PNAS) |
| 巻: | 107 |
| 号: | 51 |
| 開始ページ: | 22190 |
| 終了ページ: | 22195 |
| 抄録: | Ig class switch recombination (CSR) requires expression of activation-induced cytidine deaminase (AID) and transcription through target switch (S) regions. Here we show that knockdown of the histone chaperone facilitates chromatin transcription (FACT) completely inhibited S region cleavage and CSR in IgA-switch-inducible CH12F3-2A B cells. FACT knockdown did not reduce AID or S region transcripts but did decrease histone3 lysine4 trimethylation (H3K4me3) at both the Sμ and Sα regions. Because knockdown of FACT or H3K4 methyltransferase cofactors inhibited DNA cleavage in H3K4me3-depleted S regions, H3K4me3 may serve as a mark for recruiting CSR recombinase. These findings revealed an unexpected evolutionary conservation between CSR and meiotic recombination. |
| 著作権等: | ©2010 by the National Academy of Sciencesこの論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。This is not the published version. Please cite only the published version. |
| URI: | http://hdl.handle.net/2433/141990 |
| DOI(出版社版): | 10.1073/pnas.1016923108 |
| PubMed ID: | 21139053 |
| 出現コレクション: | 学術雑誌掲載論文等 |
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