Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. (original) (raw)
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| タイトル: | Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. |
|---|---|
| 著者: | Takahashi, Kazutoshi  Yamanaka, Shinya  |
| 著者名の別形: | 高橋, 和利山中, 伸弥 |
| 発行日: | 25-Aug-2006 |
| 出版者: | Elsevier Inc. |
| 誌名: | Cell |
| 巻: | 126 |
| 号: | 4 |
| 開始ページ: | 663 |
| 終了ページ: | 676 |
| 抄録: | Differentiated cells can be reprogrammed to an embryonic-like state by transfer of nuclear contents into oocytes or by fusion with embryonic stem (ES) cells. Little is known about factors that induce this reprogramming. Here, we demonstrate induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions. Unexpectedly, Nanog was dispensable. These cells, which we designated iPS (induced pluripotent stem) cells, exhibit the morphology and growth properties of ES cells and express ES cell marker genes. Subcutaneous transplantation of iPS cells into nude mice resulted in tumors containing a variety of tissues from all three germ layers. Following injection into blastocysts, iPS cells contributed to mouse embryonic development. These data demonstrate that pluripotent stem cells can be directly generated from fibroblast cultures by the addition of only a few defined factors. |
| 記述: | This article is related to the Nobel Prize in Physiology or Medicine 2012 |
| 著作権等: | Copyright © 2006 Elsevier Inc. All rights reserved.This posting is granted exceptional permission by the owner of copyright. |
| URI: | http://hdl.handle.net/2433/159777 |
| DOI(出版社版): | 10.1016/j.cell.2006.07.024 |
| PubMed ID: | 16904174 |
| 出現コレクション: | 学術雑誌掲載論文等 |
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