Genome Sequencing of Pediatric Medulloblastoma Links Catastrophic DNA Rearrangements with TP53 Mutations (original) (raw)

. Author manuscript; available in PMC: 2013 Jan 20.

SUMMARY

Genomic rearrangements are thought to occur progressively during tumor development. Recent findings, however, suggest an alternative mechanism, involving massive chromosome rearrangements in a one-step catastrophic event termed chromothripsis. We report the whole-genome sequencing-based analysis of a Sonic-Hedgehog medulloblastoma (SHH-MB) brain tumor from a patient with a germline TP53 mutation (Li-Fraumeni syndrome), uncovering massive, complex chromosome rearrangements. Integrating TP53 status with microarray and deep sequencing-based DNA rearrangement data in additional patients reveals a striking association between TP53 mutation and chromothripsis in SHH-MBs. Analysis of additional tumor entities substantiates a link between TP53 mutation and chromothripsis, and indicates a context-specific role for p53 in catastrophic DNA rearrangements. Among these, we observed a strong association between somatic TP53 mutations and chromothripsis in acute myeloid leukemia. These findings connect p53 status and chromothripsis in specific tumor types, providing a genetic basis for understanding particularly aggressive subtypes of cancer.

INTRODUCTION

Li-Fraumeni syndrome (LFS, OMIM #151623) is an autosomal dominant hereditary disorder that is associated with a greatly increased susceptibility to cancer (Li and Fraumeni, 1969). Most LFS patients harbor heterozygous germline mutations of TP53, the gene encoding the p53 tumor suppressor (Malkin et al., 1990). Mutant p53 can prime cells for tumor development due to aberrant cell cycle control and DNA damage response signals, and a failure to induce cell cycle arrest, senescence or apoptosis (Forbes et al., 2011; Vogelstein et al., 2000).

In a pilot study within the International Cancer Genome Consortium (ICGC) Pediatric Brain Tumor Research Project (www.pedbraintumor.org) we analyzed a medulloblastoma and paired normal sample from an LFS patient by whole-genome sequencing. Medulloblastoma is the most common malignant brain tumor of childhood, and the malignancy causing the highest cancer-related mortality in children. Medulloblastoma belongs to the spectrum of recognized LFS tumors (Taylor et al., 2000). Recent molecular studies have revealed that medulloblastoma comprises at least four distinct sub-entities, which differ in terms of cell-of-origin, clinicopathologic features, and disease outcome (Cho et al., 2011; Gibson et al., 2010; Kool et al., 2008; Northcott et al., 2011b; Remke et al., 2011). The investigated patient had a Sonic-Hedgehog subtype medulloblastoma (SHH-MB), a tumor arising from the external granular layer cells of the cerebellum (Bühren et al., 2000; Wechsler-Reya and Scott, 1999). SHH-MBs form the clinically and histopathologically most heterogeneous medulloblastoma subtype, making it challenging to predict response to therapy and patient outcome solely based on subtype affiliation (Northcott et al., 2011a; Remke et al., 2011). Cancer genome sequencing efforts underway will likely enable further refinements in medulloblastoma subtype classification, and provide novel molecular signatures for patient stratification.

Unexpectedly, the tumor genome showed massive genomic rearrangements consistent with a reshuffling of genetic material from individual chromosomes, in keeping with the recently proposed chromothripsis model for tumorigenesis (Greek; chromo from chromosome; thripsis, for shattering into pieces; Stephens et al., 2011). Chromothripsis, which is thought to occur in 2–3% of cancers (with an incidence of ~25% in bone cancers; Stephens et al., 2011), involves the acquisition of numerous rearrangements through a single catastrophic event, fundamentally different from the stepwise (progressive) acquisition of alterations by tumor cells (Fearon and Vogelstein, 1990; Nowell, 1976). In this study, we integrate whole genome sequencing and array-based approaches to further characterize this rearrangement phenomenon, and discover a novel link between chromothripsis and TP53 mutations, providing evidence that the status of p53 can influence catastrophic DNA rearrangements in a cell context-specific manner.

RESULTS

Whole-genome sequencing of a medulloblastoma in a Li-Fraumeni syndrome patient revealed highly complex DNA rearrangements

We initially analyzed an SHH-MB and paired normal tissue sample from a female LFS patient (LFS-MB1), who harbored a hereditary TP53 mutation (Table S1) predicted to eliminate p53 DNA binding activity (Rieber et al., 2009). We performed whole-genome paired-end sequencing followed by DNA sequence variant discovery (Table 1). We searched for single nucleotide variants (SNVs) by directly evaluating the alignment of DNA reads onto the human reference genome (Depristo et al., 2011; Li et al., 2009) and identified large-scale rearrangements by paired-end mapping (Korbel et al., 2007), split-read analysis (Ye et al., 2009), and read-depth analysis (Abyzov et al., 2011; Chiang et al., 2009; Waszak et al., 2010).

Table 1.

Whole-genome sequencing and DNA sequence variant statistics

The inherited TP53 mutation was detected in both tissue samples. Furthermore, we identified 24 tumor-specific SNVs that were predicted to alter protein-coding sequences (Tables 1, S1 and Figure 1A). This is slightly more than was observed in a recent study focusing on exonic regions in sporadic medulloblastomas (average 5.7 non-synonymous SNVs per sample; range 1–17 (Parsons et al., 2011)). These differences could reflect an increased genome-wide mutation rate in LFS patients or could be the result of a comparably higher sensitivity of our whole-genome sequencing approach. Using PCR we verified 20 out of 21 SNVs for which PCR primers could be designed (>95%). We did not observe any somatic small insertions or deletions (<50bp) in protein-coding regions. However, we uncovered numerous large (up to megabase-scale) alterations in the tumor sample. Amongst these were distinct amplifications of SHH pathway members (MYCN and GLI2), which we verified by fluorescence in situ hybridization (FISH), and a somatic loss of the wild-type TP53 allele by deletion of the 17p chromosome arm (Table S1, Figure 1E).

Figure 1.

Analysis of LFS-MB1 revealed catastrophic DNA rearrangements consistent with chromothripsis. (A) Genome-wide distribution of somatic DNA variants. Thin orange lines in outer-most panel are non-synonymous somatic SNVs; the next panel shows isolated genomic rearrangements. Read-depth plots (log2-ratio tumor vs. germline), indicating copy-number alterations, are in black. Connecting lines show complex large-scale (e.g., inter-chromosomal) rearrangements identified by paired-end mapping. (B) Inferred double minute chromosome structure (originating segments from chromosome 4 and 14 are highlighted in panel (A)). Genes are in gray (known cancer genes are in red). (C) PCR validation of inter-chromosomal rearrangements contributing to the inferred double minute chromosome. MB, medulloblastoma; GL, germline. (D) FISH validation of rearrangements contributing to double minute chromosome derived from chromosome 3 segments. Probes match to normally distal regions of chromosome 3 (RP11-553D4, red, and RP11-265F19, light green; see panel (A) and Figure S1). (E) Amplification of MYCN (red) and GLI2 (light green), not associated with chromothripsis (amplicon loci highlighted in panel (A) with red and light green boxes), was observed in distinct subpopulations of cells.

The most striking feature in LFS-MB1, however, was a pattern of complex somatic rearrangements that was markedly different from aberrations that we, and others have previously described in medulloblastoma (Cho et al., 2011; Northcott et al., 2009; Pfister et al., 2009). This included multiple highly amplified genomic segments, which were clustered on individual chromosome arms (Figure 1A), resulting in frequent alternations between a normal disomic copy-number state and an extreme state with a segmental copy-number of up to 30. On chromosome 3, three segments were highly amplified, whereas on chromosome 4, six segments were amplified and on chromosome 14, four segments were amplified. Based on the mapping of sequenced paired-ends, we determined physical connections linking the amplified sequences on chromosome 3 (intra-chromosomal), as well as the amplified segments from chromosomes 4 and 14 (inter-chromosomal). Strikingly, the amplified fragments from chromosomes 4 and 14 appeared fused together to form a circular 1.2Mb extra-chromosomal structure (so-called “double minute” chromosome) consisting of a complex medley of inter- and intra-chromosomal junctions (Figure 1B). Using PCR, we verified a series of these connections, including all four inter-chromosomal links (Figure 1C, Table S1). FISH experiments also confirmed the predicted co-localization of highly amplified segments on chromosome 3, indicating that these fragments formed yet another double minute chromosome (Figures 1D, S1), as predicted by the sequencing data.

It is unlikely that these extrachromosomal structures occurred as a consequence of progressive genomic alterations. Double minute chromosomes have been described to typically encompass segments from confined genomic regions, rather than from several distinct regions of a chromosome or multiple chromosomes (Storlazzi et al., 2010). The recently described chromothripsis phenomenon, however, may explain the observed complex rearrangements, as this phenomenon can lead to the formation of double minute chromosomes containing fragments from multiple regions (Stephens et al., 2011). The chromothripsis model can further explain the strikingly similar, alternating copy-number states of adjacent amplicons and the medley of relative orientations with which DNA fragments rearranged.

DNA copy-number profiling of additional medulloblastomas reveals a link between TP53 mutations and chromothripsis in SHH-MB

Previous studies have shown that evidence for chromothripsis can be inferred from SNP microarray-based DNA copy-number profiles (Stephens et al., 2011). To assess the occurrence of chromothripsis in a broader series, we analyzed SNP array data from 98 medulloblastomas, including samples from all four molecular subtypes (Table S2). We also carried out TP53 sequencing in all tumors. Specifically, we analyzed 73 samples with previously published SNP array data (Northcott et al., 2009), and generated additional SNP array data for 25 tumors, including nine with known TP53 mutations. To infer the occurrence of chromothripsis, we required at least 10 changes in segmental copy-number involving two or three distinct copy-number states on a single chromosome (see Experimental Procedures).

The vast majority of tumors displayed considerably fewer than ten copy-number state changes per chromosome (Figure 2, Data S1). A subset of cases, however (13/98), showed rearrangements consistent with chromothripsis (Figure 2, Data S1). Notably, eleven of these thirteen cases were SHH-MBs, and ten out of the eleven SHH-MBs harbored TP53 mutations, whereas three cases (including one SHH-MB and two Group 4 subtype medulloblastomas) displayed a single copy _TP53_-loss through hemizygous 17p deletion (Figure 2). Strikingly, all (10/10) SHH-MBs with mutant TP53 displayed rearrangements consistent with chromothripsis, whereas none (0/22) of the wild-type (TP53+/+) SHH-MBs showed evidence for chromothripsis (Figure 2, Data S1). This difference is highly significant (_p_=1.6×10−8, two-tailed Fisher’s exact test), indicating that mutant TP53 is strongly associated with chromothripsis in SHH-MB.

Figure 2.

SNP array copy-number profiles reveal a link between TP53 mutation and chromothripsis in SHH-MB. (A) Maximum number of copy-number state changes, and amplicon counts, on most strongly affected (rearranged) chromosomes. Abbreviation: hemiz., hemizygous. Further details are available as Data S1. Random noise was added (i.e., addition, or subtraction, of <0.5 copies) for visualization purposes. (**B**) Boxplot with maximum copy-number state changes on most strongly affected chromosome for different tumor subtypes and _TP53_ statuses. *An outlying point observed in LFS-MB3 (>200 copy-number state changes) was omitted for visualization purposes.

The ten mutant TP53 tumors with chromothripsis included two cases with heterozygous TP53 mutation (TP53+/m) and eight cases with loss of the wild-type TP53 allele (_TP53_m/−). By comparison, only one of three SHH-MBs in our cohort harboring a TP53+/− genotype (hemizygous 17p deletion) showed rearrangements consistent with chromothripsis. Whereas _TP5_3 mutation can impair or reduce the function of p53 due to dominant negative activity, hemizygous TP53 deletion may not have as significant an effect on protein function, since p53 expression levels are primarily regulated post-translationally (reviewed in Vogelstein et al., 2000).

The other three medulloblastoma subtypes rarely or never displayed rearrangements consistent with chromothripsis. In the Group 4 medulloblastoma subtype, 2/26 cases showed a copy-number pattern consistent with chromothripsis (both in the context of hemizygous TP53 deletion). In the Group 3 subtype, we did not observe evidence for chromothripsis amongst 26 analyzed tumors (Figure 2AB). No TP53 mutations were observed in either Group 3 or Group 4 tumors. WNT subtype tumors also did not display any evidence for chromothripsis (0/11), despite the known association of TP53 mutations with this subgroup (Pfaff et al., 2010) and 4/11 cases harboring a TP53 mutation. These results indicate that the link between p53 status and chromothripsis in medulloblastoma is context-specific.

The inferred association between TP53 and chromothripsis in SHH-MB makes it tempting to hypothesize that these catastrophic DNA rearrangements may have occurred as a consequence of preceding TP53 mutations. To examine whether other medulloblastoma patients with chromothripsis harbored germline mutations, we analyzed constitutional DNA samples for those TP53 mutant cases where paired normal tissue was available. We were able to retrieve germline DNA for 3 of the 4 TP53 mutated WNT tumors, and 6/10 TP53 mutated SHH tumors. Strikingly, whereas the TP53 mutations in the WNT group were all somatically acquired (3/3), most of the tested germline samples from the SHH group (5/6) harbored the same TP53 alterations as their paired tumors. Thus, in addition to linking p53 status to complex chromosomal rearrangements, we also identified five previously undiagnosed LFS cases amongst the SHH-MBs showing chromothripsis, which we herein refer to as LFS-MB2 – LFS-MB6 (Table S2).

We used deep sequencing, followed by counting DNA reads that identify mutant or wild-type TP53 alleles, to analyze the abundance of mutant TP53 relative to wildtype TP53 in several tumors. This analysis followed the rationale that a high mutant allele frequency can indicate an early mutational event, whereas a low frequency indicates late occurrence – an analysis that may facilitate an evaluation of cause versus consequence. We used this approach in four SHH-MBs with germline TP53 mutation (LFS-MB1 – LFS-MB4), the SHH-MB with confirmed somatic TP53 mutation and chromothripsis (MB2034), and two TP53 mutated WNT medulloblastomas (Supplemental Experimental Procedures). The mutant allele frequencies of the LFS-associated SHH-MBs were 87–100% (Table S2), suggesting that loss of the wild-type TP53 allele is typically an early event in SHH-MBs seen in LFS patients. Whereas the WNT subtype medulloblastomas showed comparably lower mutant TP53 allele frequencies (35–48%), all (14/14; 100%) DNA reads of the SHH-MB tumor MB2034 mapped to the mutant TP53 allele. Absence, or very low frequency, of the TP53 wild-type allele suggests that the somatic TP53 mutation likely occurred as a very early (possibly initiating) event during medulloblastoma tumorigenesis in MB2034.

DNA copy-number profiles of medulloblastoma mouse models substantiates the link between TP53 and chromothripsis in SHH-MB

We further reanalyzed published microarray data from two SHH-MB mouse models. The mice were hemizygous for patched (Ptch+/−), which promotes the development of SHH-MB-like tumors (Buonamici et al., 2010; Ishida et al., 2010). One study examined tumor growth in mice receiving medulloblastoma tumors (allografts) from a donor mouse lacking the murine p53 ortholog (Trp53) and a copy of patched (Buonamici et al., 2010) whereas another study examined tumors from Ptch+/−;Trp53+/+ mice (Ishida et al., 2010). All six tumors in mice receiving allografts from the Ptch+/−;Trp53−/− donor showed rearrangements consistent with chromothripsis (Data S2). Chromosome 16 was affected in each case, suggesting that chromothripsis had occurred already in the donor. 2/6 recipient mice further acquired alterations consistent with chromothripsis on other chromosomes (Supplemental Experimental Procedures). By comparison, none (0/3) of the Ptch+/− ;Trp53+/+ mice showed chromothripsis (Data S2). These findings provide additional support for a link between chromothripsis and p53 in SHH-MB.

Whole-genome sequencing of three additional TP53 mutated SHH-MBs verifies the occurrence of chromothripsis

We next subjected SHH-MB and paired normal tissue samples from three of the newly identified LFS patients (LFS-MB2 – LFS-MB4) to additional analysis by whole-genome sequencing, using the same approach as with LFS-MB1. One case (LFS-MB3) was sequenced to a lower whole-genome coverage (Table 1) sufficient for confirming the presence of chromothripsis, complemented by deep sequencing of exonic regions using a targeted exome capture approach. To better facilitate a detailed characterization of the massive rearrangements seen in chromothripsis, we additionally used long-range paired-end mapping (Korbel et al., 2007), generating deep physical genomic coverage of the two samples for which sufficient DNA was available (Table 1).

We identified 11–24 non-synonymous somatic SNVs in the three tumor samples (Tables 1, S3, S4) – again higher than the rate previously reported in sporadic medulloblastoma (Parsons et al., 2011). The only recurrently altered gene we identified was TP53, which was confirmed to be mutated in the germline in all cases. Importantly, the genome sequencing data further verified the SNP array-based inference of chromothripsis in all tumors. Specifically, LFS-MB2 showed chromothripsis on chromosomes 3 and X, with resulting amplicons that were physically connected to form a complex double minute chromosome (Figure 3AB). Furthermore, chromosomes 8 and 17 displayed multiple alternations between few copy-number states, involving a crisscrossing between one, two and three copies (Figure 3A) – a rearrangement pattern that is also typical for chromothripsis (Stephens et al., 2011). In LFS-MB3, chromosome 15 displayed one of the most striking genomic alteration patterns in our sample set, with >100 highly-rearranged segments (Figure 3D). LFS-MB4 showed rearrangements consistent with chromothripsis on chromosomes 2 and 12, with several of the chromosome 2 segments forming another double minute chromosome (Figure 4AB).

Figure 3.

Verification of chromothripsis in LFS-MB2 and LFS-MB3 by whole-genome sequencing. (A) Copy-number profiles in LFS-MB2. (B) Predicted double minute chromosome structure. *Segments connected to centromere-like repeat sequences. (C) FISH confirming co-localization of fragments from chromosomes 3 and X in LFS-MB2 (RP11-245A6, green and CTD-2530H13, red); positions of FISH probes are shown on the inner-most circle of panel (B)). PCR experiments also confirmed this co-localization (see Figure S2). (D) Read-depth plot showing chromosome 15 rearrangements resulting from chromothripsis in LFS-MB3. Links connecting amplified regions are displayed on top (other links involving rearranged segments are displayed at the bottom).

Figure 4.

Verification of chromothripsis in LFS-MB4. (A) DNA copy-number profiles. (B) Inferred double minute chromosome structure with SHH pathway members MYCN and GLI2. (C) PCR confirms juxtaposition of segments shown in panel (B). (D) FISH verifying co-localization of MYCN (red) and GLI2 (green).

We performed experimental validations, overall 27 PCRs and 3 FISH experiments, on all four SHH-MBs in which we carried out whole-genome sequencing. These experiments verified the chromothripsis-associated rearrangements inferred by paired-end mapping (Figures 1, 3, 4, S2). The FISH experiments further confirmed the presence of double minute chromosomes in nearly all tumor cells. In conjunction with the high magnitude of log-ratio shifts in the context of single-copy alterations resulting from chromothripsis (Figure 3AD, 4A, Data S1), this finding indicates an early occurrence of chromothripsis in SHH-MB tumorigenesis rather than a later, sub-clonal event.

Known medulloblastoma oncogenes are frequently amplified as a result of chromothripsis

Given the high incidence of chromothripsis in LFS-associated SHH-MB, we speculated that this phenomenon is a key driver of SHH-MB tumorigenesis. We observed an enrichment of oncogenes (2.3-fold enrichment; P<0.03; Fisher’s exact test), and more specifically of oncogenes involved in SHH signaling (30-fold enrichment; P<0.0002; see Table 2), amongst highly amplified segments rearranged by chromothripsis. The abundance of oncogenes likely explains the observed high copy-numbers of rearranged structures such as double minute chromosomes – presumably resulting from unequal segregation during cell division and subsequent selection for a growth advantage of malignant cells.

Table 2.

Chromothripsis-associated amplified regions typically contain medulloblastoma oncogenes

Notably, in LFS-MB4 the chromothripsis-associated rearrangements brought two SHH signaling genes on chromosome 2 – i.e., MYCN and GLI2 – into close proximity (Figure 4). This pattern is in contrast to LFS-MB1, where the SHH-MB oncogenes MYCN and GLI2 were amplified in different tumor cell subpopulations, and where chromosome 2 was not affected by chromothripsis (Figure 1). Furthermore, in LFS-MB1 we observed another SHH signaling gene, BOC, amplified in association with chromothripsis (Table 2 and Figure S1).

In two patients, the chromothripsis-associated rearrangements led to the formation of putative in-frame fusions involving genes previously reported to be dysregulated in cancer (Table 2, Figures 4B). One of these genes, NEB, was recently reported to harbor somatic point mutations in medulloblastoma (Parsons et al., 2011), suggesting that the resulting fusion gene may play a crucial role in the respective tumor.

Characterization of the molecular nature of the catastrophic DNA rearrangements with complementary computational approaches

To further characterize these complex rearrangements we examined the visually apparent genomic clustering of amplified regions, which were frequently found on single chromosome arms, using computational simulations. We observed that segments contributing to inter-connected and highly amplified (>10-fold change in copy number) loci displayed pronounced topological clustering in all four sequenced SHH-MBs (Figure 5A), consistent with previous reports in the context of chromothripsis (Stephens et al., 2011).

Figure 5.

Analysis of chromothripsis-associated DNA rearrangements in SHH-MB and AML. (A) Topographical clustering of amplified regions rearranged by chromothripsis. Sim., simulated amplicon distances (_P_-values are based on 1000 permutations). (B) Simulations of progressive rearrangements segregate from the actual data in terms of mean excess in copy-number compared to unaffected regions. (C) Rearrangement formation mechanisms analysis. Polymorphic genomic structural variants detected in the germline are shown for comparison. _P-_values, indicating significant differences between the distributions of inferred formation mechanisms, are based on Chi-square tests. VNTR, expansion or shrinkage of regions with variable number of tandem repeats; MEI, mobile element insertions; NAHR, non-allelic homologous recombination (other abbreviations: see main text). (D) Somatically acquired TP53 mutations are linked with the occurrence of chromothripsis in AML. Black filled circles: AMLs with chromothripsis. Gray open circles: AMLs without chromothripsis. Example copy-number profiles are available as Data S2, and a detailed summary of the AML data is in Table S5. (E) Cancer gene enrichment in association with chromothripsis in SHH-MBs, analyzed by deep sequencing, and AMLs, analyzed by SNP arrays. Regions hemizygously deleted (‘lost’), gained, and highly (>10-fold) amplified as a consequence of chromothripsis were separately analyzed. No genes displayed high-level amplification in AML in association with chromothripsis (‘_NA_’). ‘*’significant based on Fisher’s exact test.

Simulations were also used to evaluate the possibility that the observed patterns of vastly alternating normal disomic and “extreme” (highly amplified) copy-number states may be explained by progressive rearrangements. We analyzed chromosomes with inferred chromothripsis using 1000 simulations each, by progressively, and randomly, introducing all detected inter-segmental-connections that involved highly amplified regions into the respective patients’ germline background in a stepwise fashion (see Experimental Procedures). In all four cases, based on the results of the simulations, a progressive rearrangement model was rejected, thereby implicating the occurrence of chromothripsis. In each case the simulations resulted in less pronounced as well as less regular switches between copy-number states than the actual data (p<0.005; based on permutations; Figure 5B).

Analysis of breakpoint sequence signatures (Lam et al., 2010) revealed short microhomology tracts (≤4bp), compatible with non-homologous end-joining (NHEJ)-mediated double-strand repair, or microhomology-mediated break-induced replication (MMBIR) (Hastings et al., 2009a; Lee et al., 2007), for the vast majority of breakpoints (Figure 5C). In a few cases, short insertions of non-template DNA sequence were observed at the breakpoint junctions (Table S5). NHEJ-mediated repair of shattered DNA fragments appears more likely to be involved in this context, as it is difficult to reconcile the model of MMBIR with the massive rearrangements (including complex double minute chromosomes) we detected in association with chromothripsis. Furthermore, we did not identify templated insertions – commonly observed in association with replication-based rearrangement mechanisms (such as MMBIR) – at the breakpoint junctions of chromothripsis-associated rearrangements.

TP53 mutation is linked to chromothripsis in acute myeloid leukemia

To investigate a potential association between TP53 mutations and chromothripsis in LFS-associated tumors other than medulloblastoma, we collected copy-number profile data for tumors from eleven LFS patients (Supplemental Experimental Procedures), including adrenocortical carcinoma (_n_=4), rhabdomyosarcoma (_n_=4), extrarenal rhabdoid tumor (_n_=1), neuroblastoma (_n_=1), and glioblastoma (_n_=1). In all eleven patients, DNA sequencing identified the presence of a TP53 mutation in the germline. Four out of the eleven (36%) tumors showed rearrangements consistent with chromothripsis (Table S2 and Data S1). While larger sample sizes of these rare tumor entities are required for formal testing, our results indicate that TP53 germline mutations could be linked with a high incidence of chromothripsis in LFS-associated malignancies other than medulloblastoma.

The occurrence of chromothripsis in an SHH-MB with somatic TP53 mutation led us to further investigate relationships between p53 and chromothripsis in other malignancies with available high-resolution SNP microarray and somatic TP53 status. We obtained such data for acute myeloid leukemia (AML) (Parkin et al., 2010). Previous studies have reported that mutations in TP53 correlate with “complex” karyotypes in AML (Haferlach et al., 2008). The TP53 mutations in the AML patient cohort were somatically acquired as assessed using paired constitutional DNA (Parkin et al., 2010). Copy-number profiling revealed an abundance of massive, complex rearrangements in the TP53 mutated AMLs, with numerous alternating copy-number state switches involving two or three copy-number states, that were specifically localized to single chromosomes (Figure 5D, Table S5). These rearrangements were markedly different from the pattern of general genomic instability observed in prototypical TP53 mutated tumors (Data S2), and implicated chromothripsis in the tumorigenesis of TP53 mutated AMLs. In total, 8/17 (47%) AMLs with a somatically acquired TP53 mutant genotype, but only 1/91 (1%) AMLs with wild-type TP53 (TP53+/+), displayed patterns of rearrangement consistent with chromothripsis, a finding that links somatic TP53 mutations with chromothripsis in AML (_P_=5.7 × 10−7; Fisher’s exact test).

We further analyzed a previously unpublished SNP microarray dataset from a cohort of 311 AML patients with available karyotypic subgrouping. These included 56 “complex” karyotype AMLs (Haferlach et al., 2008), 46% of which harbored TP53 mutations, and an additional 18% of which harbored a TP53+/− genotype. Our analysis revealed a similar abundance of chromothripsis amongst TP53 mutated tumors as in the aforementioned AML cohort. When applying our strict definition for inferring chromothripsis, approximately half of the TP53 mutated tumors showed chromothripsis (Data S2, Table S5), while none of the cases classified as “non-complex” karyotype AML – which exhibit TP53 mutations in only ~2% of cases (Haferlach et al., 2008) – harbored alterations resulting from chromothripsis. An additional 12% of TP53 mutated AMLs harbored rearrangements reminiscent of chromothripsis that fell just below our conservative scoring threshold (Supplemental Experimental Procedures).

We additionally tested for enrichment of cancer-related genes in regions rearranged by chromothripsis in AML. In contrast to SHH-MBs, the AML samples did not harbor high-level amplifications in association with chromothripsis, and instead showed hemizygous deletions of tumor suppressors (Figure 5E), some of which have known roles in AML, including FHIT, NBN, and IRF1 (Table S5). Further analysis of gene expression profiles in these AML samples (Supplemental Experimental Procedures) – including cases with and without evidence for chromothripsis – did not reveal evidence for SHH pathway activation (Data S2), as was seen in _TP53_-mutated SHH-MBs with chromothripsis. Thus, in AML chromothripsis is linked with somatically acquired TP53 mutations, but not with SHH pathway activation.

The availability of clinical follow-up data for the AML patients also enabled us to test for an association of chromothripsis with poor prognosis. We tested for such an association in both aforementioned AML cohorts, controlling for patient age at diagnosis, since AMLs harboring “complex” karyotypes display an increased incidence in patients with advanced age (Mrozek, 2008). Indeed, we found that chromothripsis in AML is associated with poor survival (P<0.0001; Wald’s test; Data S2), an association that remained significant independent from patient age, and “complex” karyotype classification, when assessed in multivariate analyses. These tests also revealed an increased age of diagnosis in _TP53_-mutated AMLs with chromothripsis (median=62 years (yr); mean=67 yr), compared to _TP53_-mutated AMLs not showing chromothripsis (median=57 yr; mean=58 yr; _P_=0.04; KS-test).

DISCUSSION

Integrated analysis of DNA sequencing and microarray data enabled us to discover an association between mutations of a specific gene, i.e. TP53, with chromothripsis. The unexpected germline nature of mutant TP53 in several LFS-associated SHH-MBs means that the TP53 mutation must precede the massive shattering and chromosome rearrangements in these. This led us to propose a novel role for p53 in the initiation of, or cellular reaction to, chromothripsis – with TP53 mutations predisposing cells for chromothripsis, or facilitating cell survival following catastrophic DNA rearrangements (Figure 6). The high frequency of TP53 germline mutations specifically in SHH tumors further suggests that LFS patients may be predisposed to SHH-MB, rather than to medulloblastoma per se. This finding has clinical implications, and we recommend assessing the merit of testing SHH-MB patients with chromothripsis for germline TP53 mutations, since regular screening in LFS families was recently shown to lead to a survival benefit (Villani et al., 2011). Additionally, particular care may be required with respect to current treatment regimens, which in most cases include DNA damaging agents and radiotherapy. These may induce therapy resistance in the primary tumor or trigger secondary malignancies in the context of constitutional TP53 mutations and deficient DNA repair.

Figure 6.

New model linking TP53 mutation status to catastrophic DNA rearrangements. Related references: 1Tabori et al. (2007); 2Tusell et al. (2010); 3Meyerson and Pellman (2011); 4Vogelstein et al. (2000); 5Dahm-Daphi et al. (2005).

The frequent occurrence of chromothripsis in the context of germline alterations of TP53 points to a possible requirement of TP53 mutations in the tumor cell-of-origin, or of TP53 mutations acquired early in tumor development. The identification of chromothripsis in the context of somatic TP53 mutations in an SHH-MB patient and in several AMLs additionally implicates acquired, tumor-specific TP53 mutations in chromothripsis.

Possible explanations for the absence of chromothripsis in several malignancies with TP53 mutations, including several AMLs and WNT medulloblastomas, are: their comparably late acquisition of such mutations; the occurrence of TP53 mutations in tumor subpopulations; and cell context-specific factors, including distinct gene expression programs. Further to this, selective growth advantages are likely to determine tumor development following chromothripsis. The higher rate of chromothripsis in _TP53_-mutated SHH-MBs (all cases we analyzed) compared to _TP53_-mutated AMLs (approximately half of the cases we analyzed) is striking. It is tempting to speculate that this difference is connected with the abundance of high-level oncogene amplifications, typically involving double minute chromosomes, in SHH-MBs with chromothripsis. The selective advantage of such oncogene amplification is likely higher than the selective advantage conferred by hemizygous tumor suppressor gene loss observed in AML. Furthermore, rare cases of chromothripsis in AMLs harboring wild-type TP53, and in medulloblastomas with hemizygous TP53 deletion, show that chromothripsis can also occur in the absence of TP53 point mutations – perhaps in association with low TP53 gene dosage, or other genetic or epigenetic mechanisms causing p53 pathway dysregulation.

Our analyses of rearrangement breakpoints are in support of a model of massive DNA double strand breaks (Stephens et al., 2011), followed by NHEJ-mediated repair (Figure 6). The replication-associated mechanism MMBIR can also generate alterations with multiple breakpoints (Hastings et al., 2009a). The lack of templated insertions at the breakpoint junctions, however, which are thought to result from abortive attempts to use another template during replication (Hastings et al., 2009b; Howarth et al., 2011), does not substantiate the involvement of a replication-associated rearrangement mechanism in the complex alterations we observed.

Stephens et al. (2011) have discussed two possible scenarios that may underlie the chromosome shattering and rearrangement seen in chromothripsis. Both scenarios involve damage occurring in mitotic chromosomes, since the compaction of chromosomes during mitosis could explain the highly localized nature of the DNA breaks, which are often focused on a single chromosome. We note, however, that the known spatial organization of chromosomes maintained during interphase (Cremer and Cremer, 2001; Lichter et al., 1988) might similarly represent a structural basis for the local occurrence of DNA shattering. In one scenario, it was proposed by Stephens et al. that ionizing radiation may lead to a catastrophic series of DNA double strand breaks. Based on our observations, these breaks might be preferentially repaired by low-fidelity mechanisms, such as error-prone NHEJ, as these are known to play a greater role when levels of p53 activity are reduced (Dahm-Daphi et al., 2005). An additional result of impaired p53 activity could be an increased rate at which cells are able to survive, and divide, after acquiring catastrophic chromosome alterations.

In a second possible scenario, critical telomere shortening followed by chromosome end-to-end fusions (which are prone to occur in association with uncapped telomeres (Tusell et al., 2010)) and subsequent breakage could lead to chromothripsis. Furthermore, dividing cells with uncapped telomeres are delayed at the G2/M transition in a p53-dependent manner, and unprotected telomeres in p53-deficient cells undergoing mitosis were found to be shorter than average, and prone to form end-to-end fusions (Thanasoula et al., 2010). Intriguingly, LFS patients harbor shortened telomeres compared to unaffected individuals of the same age group, with average telomere length decreasing from generation to generation in affected families, and the age of onset of cancer showing an association with average telomere length (Tabori et al., 2007; Trkova et al., 2007). As two different chromosomes may participate in an end-to-end fusion, such events could explain our observations of chromothripsis leading to complex inter-chromosomal rearrangements (with derived double minute chromosomes), which involved no more than two chromosomes. Thus, critical telomere shortening followed by end-to-end fusions and subsequent tearing apart during mitosis provides a plausible explanation for the link between TP53 mutations and chromothripsis reported in this study. The finding that chromothripsis frequently occurred in individuals with advanced age at diagnosis in _TP53_-mutant AML is also of note, given the progressive shortening of telomeres with age.

Additional contributions of p53 in controlling the G2/M transition checkpoint (Kastan and Bartek, 2004) suggest that the involvement of a different mechanism – premature chromosome compaction – may also be a possibility. In this process chromosomes from an S-phase nucleus condense prematurely and, as a result, may become shattered (Meyerson and Pellman, 2011). Given the multiple and varied roles that have been assigned to p53, it is possible that the link between TP53 and chromothripsis is associated with more than one of the aforementioned functions acting in concert (Figure 6).

In contrast to earlier reports on the rarity of chromothripsis (Kloosterman et al., 2011; Stephens et al., 2011), we found that in specific biological contexts this phenomenon occurs at high frequency. In conclusion, results from our study – which reported the first whole-genome sequence data for tumors from LFS patients and for pediatric brain tumors – indicate a new role for p53, a protein also known as the ‘guardian of the genome’. Results from upcoming large-scale cancer genome sequencing studies (The International Cancer Genome Consortium, 2010) may shed light on additional factors associated with this catastrophic genomic phenotype.

EXPERIMENTAL PROCEDURES

Patients

Informed consent and an ethical vote (Institutional Review Board) were obtained according to ICGC guidelines (www.icgc.org). No patient underwent chemotherapy or radiotherapy prior to the surgical removal of the primary tumor.

DNA library preparation and sequencing

DNA library preparation was carried out using Illumina, Inc., paired-end (PE) and mate-pair (MP, or long-range paired-end mapping) protocols. In brief, 5ug (PE) or 10ug (MP) of genomic DNA isolations were fragmented to ~300bp (PE) insert-size with a Covaris device, or to ~4kb (MP) with a Hydroshear device, followed by size selection through agarose gel excision. Deep sequencing was carried out with Genome Analyzer IIx and HiSeq2000 instruments. Exome capturing was carried out with Agilent SureSelect Human All Exon 50 Mb in-solution capture reagents (vendor’s protocol v2.0.1).

Sequence variant discovery and analysis

We mapped DNA read data onto the human reference genome and subsequently detected SNVs, InDels, and genomic rearrangements with complementary computational approaches (see Supplemental Experimental Procedures).

SNP arrays

SNP arrays were hybridized, and copy-number alterations identified, as previously described (Northcott et al., 2009). We inferred chromothripsis in cases where at least ten switches between two or three copy-number states were apparent on an individual chromosome – e.g., a sequence of the states ‘2’ and ‘1’ (‘2; 1; 2; 1; 2; 1; 2; 1; 2; 1; 2’), or ten switches between ‘2’ and a highly amplified (e.g. ‘30’) state (‘2; 30; 2; 30; 2; 30; 2; 30; 2; 30; 2’). We chose this threshold as we and others have demonstrated that whereas sporadic medulloblastomas display a high degree of variability in the level of observed copy number alterations, specific patterns of alternating copy-number patterns with multiple breakpoints localized on one chromosome, involving few copy-number states, have not previously been described in these (Cho et al., 2011; Northcott et al., 2009; Pfister et al., 2009).

PCR validation

PCR validations for breakpoint junctions were performed as previously described (Korbel et al., 2007). SNVs were validated by PCR, followed by capillary sequencing in whole genome amplified (Qiagen) DNA samples.

FISH

FISH was performed as previously described (Pfister et al., 2009).

Breakpoint clustering simulation

To evaluate topographical clustering of chromothripsis-associated DNA segments we shuffled the location of amplicons (>10-fold change in copy number) along single chromosomes, using 1000 simulations each. We limited our simulations to mappable regions of the reference genome (i.e., genomic segments where >50% of the DNA reads could be uniquely aligned).

Rearrangement formation mechanism analysis

Rearrangement mechanisms were inferred with BreakSeq (Lam et al., 2010), using recently described classification parameters (Mills et al., 2011).

Simulations of the progressive rearrangement model

We simulated progressively occurring alterations based on a recently formulated computational approach (Stephens et al., 2011), as described in detail in the Supplemental Experimental Procedures.

Cancer gene enrichment analysis

Lists of cancer-related genes associated with SHH-MB and AML were compiled by supplementing a curated general cancer gene set with comprehensive lists of previously published disease-specific genes (see Table S5 and Supplemental Experimental Procedures). We then assessed whether these gene sets were enriched in regions affected by chromothripsis compared to the whole set of RefSeq genes, using Fisher’s exact test.

Supplementary Material

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Acknowledgments

We thank Bettina Haase, David Ibberson, and Dinko Pavlinic from the EMBL GeneCore sequencing team for excellent support, and Matthias Hentze and Lars Steinmetz for valuable comments on earlier versions of the manuscript. High-performance computing facilities at EMBL and DKFZ supported the study technically. The project was supported by grants from the German Cancer Aid (109252) and the BMBF (ICGC 'PedBrain' Tumor Project; to P.L., S.M.P., A.K., R.E., B.B., O.W., and J.O.K.; and 01GS0871; to L.B., K.D., and H.D.), the European Commission (Health-F2-2010-260791, to JOK), the NIH (R01CA148699b) and the Pediatric Brain Tumor Foundation (to MDT), the Canadian Institutes for Health Research (MOP-97834, to DM), and the German Research Foundation (BU 1339/3-1, to LB, and KO 4037/1-1, to JOK).

Footnotes

SUPPLEMENTAL INFORMATION

Supplemental Information includes 5 tables, 2 datasets, and 2 figures.

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