Chromatin model calculations: Arrays of spherical nu bodies. (original) (raw)
- Journal List
- Nucleic Acids Res
- v.3(1); 1976 Jan
- PMC342880
Nucleic Acids Res. 1976 Jan; 3(1): 89–100.
Abstract
Chromatin fibers consists of globular nucleohistone particles (designated nu bodies) along the length of the chromatin DNA with approximately 6-to7-fold compaction of the DNA within the nu bodies. We have calculated theoretical small-angle x-ray scattering curves and have compared these with experimental data in the literature. Several models predict maxima at the correct angles. The first maximum (approximately 110 degrees A) results from interparticle interference, while both the spatial arrangement and the structure factor the nu bodies can contribute to the additional small-angle maxima. These calculations suggest models which can account for the electron microscopic observation that chromatin is seen as either approximately 100-or approximately 200-to 250 degrees A-diameter fibers, depending on the solvent conditions. They also account for the limited orientability of the x-ray pattern from pulled chromatin fibers.
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- WILKINS MH. Physical studies of the molecular structure of deoxyribose nucleic acid and nucleoprotein. Cold Spring Harb Symp Quant Biol. 1956;21:75–90. [PubMed] [Google Scholar]
- Pardon JF, Wilkins MH, Richards BM. Super-helical model for nucleohistone. Nature. 1967 Jul 29;215(5100):508–509. [PubMed] [Google Scholar]
- Pardon JF, Wilkins MH. A super-coil model for nucleohistone. J Mol Biol. 1972 Jul 14;68(1):115–124. [PubMed] [Google Scholar]
- Subirana JA, Puigjaner LC. X-ray diffraction studies of nucleohistone: a polyhelical model of chromosome organization. Proc Natl Acad Sci U S A. 1974 May;71(5):1672–1676. [PMC free article] [PubMed] [Google Scholar]
- DuPraw EJ. Macromolecular organization of nuclei and chromosomes: a folded fibre model based on whole-mount electron microscopy. Nature. 1965 Apr 24;206(982):338–343. [PubMed] [Google Scholar]
- Davies HG. Electron-microscope observations on the organization of heterochromatin in certain cells. J Cell Sci. 1968 Mar;3(1):129–150. [PubMed] [Google Scholar]
- Everid AC, Small JV, Davies HG. Electron-microscope observations on the structure of condensed chromatin: evidence for orderly arrays of unit threads on the surface of chicken erythrocyte nuclei. J Cell Sci. 1970 Jul;7(1):35–48. [PubMed] [Google Scholar]
- Wolfe SL, Grim JN. The relationship of isolated chromosome fibers to the fibers of the embedded nucleus. J Ultrastruct Res. 1967 Aug;19(3):382–397. [PubMed] [Google Scholar]
- Brasch K, Seligy VL, Setterfield G. Effects of low salt concentration on structural organization and template activity of chromatin in chicken erythrocyte nuclei. Exp Cell Res. 1971 Mar;65(1):61–72. [PubMed] [Google Scholar]
- Ris H, Kubai DF. Chromosome structure. Annu Rev Genet. 1970;4:263–294. [PubMed] [Google Scholar]
- Golomb HM, Bahr GF. Electron microscopy of human interphase nuclei. Determination of total dry mass and DNA-packing ratio. Chromosoma. 1974;46(3):233–245. [PubMed] [Google Scholar]
- Olins AL, Olins DE. Spheroid chromatin units (v bodies). Science. 1974 Jan 25;183(4122):330–332. [PubMed] [Google Scholar]
- Olins AL, Carlson RD, Olins DE. Visualization of chromatin substructure: upsilon bodies. J Cell Biol. 1975 Mar;64(3):528–537. [PMC free article] [PubMed] [Google Scholar]
- Senior MB, Olins AL, Olins DE. Chromatin fragments resembling v bodies. Science. 1975 Jan 17;187(4172):173–175. [PubMed] [Google Scholar]
- Rill R, Van Holde KE. Properties of nuclease-resistant fragments of calf thymus chromatin. J Biol Chem. 1973 Feb 10;248(3):1080–1083. [PubMed] [Google Scholar]
- Sahasrabuddhe CG, Van Holde KE. The effect of trypsin on nuclease-resistant chromatin fragments. J Biol Chem. 1974 Jan 10;249(1):152–156. [PubMed] [Google Scholar]
- van Bruggen EF, Arnberg AC, van Holde KE, Sahasrabuddhe CG, Shaw BR. Electron microscopy of chromatin subunit particles. Biochem Biophys Res Commun. 1974 Oct 23;60(4):1365–1370. [PubMed] [Google Scholar]
- Van Holde KE, Sahasrabuddhe CG, Shaw BR. A model for particulate structure in chromatin. Nucleic Acids Res. 1974 Nov;1(11):1579–1586. [PMC free article] [PubMed] [Google Scholar]
- Oosterhof DK, Hozier JC, Rill RL. Nucleas action on chromatin: evidence for discrete, repeated nucleoprotein units along chromatin fibrils. Proc Natl Acad Sci U S A. 1975 Feb;72(2):633–637. [PMC free article] [PubMed] [Google Scholar]
- Hewish DR, Burgoyne LA. Chromatin sub-structure. The digestion of chromatin DNA at regularly spaced sites by a nuclear deoxyribonuclease. Biochem Biophys Res Commun. 1973 May 15;52(2):504–510. [PubMed] [Google Scholar]
- Burgoyne LA, Hewish DR, Mobbs J. Mammalian chromatin substructure studies with the calcium-magnesium endonuclease and two-dimensional polyacrylamide-gel electrophoresis. Biochem J. 1974 Oct;143(1):67–72. [PMC free article] [PubMed] [Google Scholar]
- Noll M. Subunit structure of chromatin. Nature. 1974 Sep 20;251(5472):249–251. [PubMed] [Google Scholar]
- Noll M. Internal structure of the chromatin subunit. Nucleic Acids Res. 1974 Nov;1(11):1573–1578. [PMC free article] [PubMed] [Google Scholar]
- Kornberg RD. Chromatin structure: a repeating unit of histones and DNA. Science. 1974 May 24;184(4139):868–871. [PubMed] [Google Scholar]
- Griffith JD. Chromatin structure: deduced from a minichromosome. Science. 1975 Mar 28;187(4182):1202–1203. [PubMed] [Google Scholar]
- Carlson RD, Olins AL, Olins DE. Urea denaturation of chromatin periodic structure. Biochemistry. 1975 Jul 15;14(14):3122–3125. [PubMed] [Google Scholar]
- Oudet P, Gross-Bellard M, Chambon P. Electron microscopic and biochemical evidence that chromatin structure is a repeating unit. Cell. 1975 Apr;4(4):281–300. [PubMed] [Google Scholar]
- Erickson RO. Tubular packing of spheres in biological fine structure. Science. 1973 Aug 24;181(4101):705–716. [PubMed] [Google Scholar]
- Bram S, Butler-Browne G, Baudy P, Ibel K. Quaternary structure of chromatin. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1043–1045. [PMC free article] [PubMed] [Google Scholar]
- Richards BM, Pardon JF. The molecular structure of nucleohistone (DNH). Exp Cell Res. 1970 Sep;62(1):184–196. [PubMed] [Google Scholar]
- Baldwin JP, Boseley PG, Bradbury EM, Ibel K. The subunit structure of the eukaryotic chromosome. Nature. 1975 Jan 24;253(5489):245–249. [PubMed] [Google Scholar]
- Pooley AS, Pardon JF, Richards BM. The relation between the unit thread of chromosomes and isolated nucleohistone. J Mol Biol. 1974 Jan 5;85(4):533–549. [PubMed] [Google Scholar]
- Henley C. Chromatin condensation involving lamellar strands in spermiogenesis of Goniobasis proxima. Chromosoma. 1973;42(2):163–174. [PubMed] [Google Scholar]
- Hill WE, Rossetti GP, Van Holde KE. Physical studies of ribosomes from Escherichia coli. J Mol Biol. 1969 Sep 14;44(2):263–277. [PubMed] [Google Scholar]
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