Sterol regulation of acetyl coenzyme A carboxylase: a mechanism for coordinate control of cellular lipid. (original) (raw)

• Journal List
• Proc Natl Acad Sci U S A
• v.93(3); 1996 Feb 6
• PMC40028

Proc Natl Acad Sci U S A. 1996 Feb 6; 93(3): 1049–1053.

Department of Molecular Biology and Biochemistry, University of California, Irvine 92717, USA.

Abstract

Transcription from the housekeeping promoter for the acetyl coenzyme A carboxylase (ACC) gene, which encodes the rate-controlling enzyme of fatty acid biosynthesis, is shown to be regulated by cellular sterol levels through novel binding sites for the sterol-sensitive sterol regulatory element binding protein (SREBP)-1 transcription factor. The position of the SREBP sites relative to those for the ubiquitous auxiliary transcription factor Sp1 is reminiscent of that previously described for the sterol-regulated low density lipoprotein receptor promoter. The experiments provide molecular evidence that the metabolism of fatty acids and cholesterol, two different classes of essential cellular lipids, are coordinately regulated by cellular lipid levels.

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Selected References

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• Hardie DG. Regulation of fatty acid and cholesterol metabolism by the AMP-activated protein kinase. Biochim Biophys Acta. 1992 Feb 12;1123(3):231–238. [PubMed] [Google Scholar]
• Yokoyama C, Wang X, Briggs MR, Admon A, Wu J, Hua X, Goldstein JL, Brown MS. SREBP-1, a basic-helix-loop-helix-leucine zipper protein that controls transcription of the low density lipoprotein receptor gene. Cell. 1993 Oct 8;75(1):187–197. [PubMed] [Google Scholar]
• Hua X, Yokoyama C, Wu J, Briggs MR, Brown MS, Goldstein JL, Wang X. SREBP-2, a second basic-helix-loop-helix-leucine zipper protein that stimulates transcription by binding to a sterol regulatory element. Proc Natl Acad Sci U S A. 1993 Dec 15;90(24):11603–11607. [PMC free article] [PubMed] [Google Scholar]
• Wang X, Sato R, Brown MS, Hua X, Goldstein JL. SREBP-1, a membrane-bound transcription factor released by sterol-regulated proteolysis. Cell. 1994 Apr 8;77(1):53–62. [PubMed] [Google Scholar]
• Tontonoz P, Kim JB, Graves RA, Spiegelman BM. ADD1: a novel helix-loop-helix transcription factor associated with adipocyte determination and differentiation. Mol Cell Biol. 1993 Aug;13(8):4753–4759. [PMC free article] [PubMed] [Google Scholar]
• López-Casillas F, Ponce-Castañeda MV, Kim KH. In vivo regulation of the activity of the two promoters of the rat acetyl coenzyme-A carboxylase gene. Endocrinology. 1991 Aug;129(2):1049–1058. [PubMed] [Google Scholar]
• Ha J, Daniel S, Kong IS, Park CK, Tae HJ, Kim KH. Cloning of human acetyl-CoA carboxylase cDNA. Eur J Biochem. 1994 Jan 15;219(1-2):297–306. [PubMed] [Google Scholar]
• Osborne TF, Gil G, Brown MS, Kowal RC, Goldstein JL. Identification of promoter elements required for in vitro transcription of hamster 3-hydroxy-3-methylglutaryl coenzyme A reductase gene. Proc Natl Acad Sci U S A. 1987 Jun;84(11):3614–3618. [PMC free article] [PubMed] [Google Scholar]
• Sanchez HB, Yieh L, Osborne TF. Cooperation by sterol regulatory element-binding protein and Sp1 in sterol regulation of low density lipoprotein receptor gene. J Biol Chem. 1995 Jan 20;270(3):1161–1169. [PubMed] [Google Scholar]
• Chirgwin JM, Przybyla AE, MacDonald RJ, Rutter WJ. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979 Nov 27;18(24):5294–5299. [PubMed] [Google Scholar]
• Metherall JE, Goldstein JL, Luskey KL, Brown MS. Loss of transcriptional repression of three sterol-regulated genes in mutant hamster cells. J Biol Chem. 1989 Sep 15;264(26):15634–15641. [PubMed] [Google Scholar]
• Maxam AM, Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. [PubMed] [Google Scholar]
• Südhof TC, Van der Westhuyzen DR, Goldstein JL, Brown MS, Russell DW. Three direct repeats and a TATA-like sequence are required for regulated expression of the human low density lipoprotein receptor gene. J Biol Chem. 1987 Aug 5;262(22):10773–10779. [PubMed] [Google Scholar]
• Kim JB, Spotts GD, Halvorsen YD, Shih HM, Ellenberger T, Towle HC, Spiegelman BM. Dual DNA binding specificity of ADD1/SREBP1 controlled by a single amino acid in the basic helix-loop-helix domain. Mol Cell Biol. 1995 May;15(5):2582–2588. [PMC free article] [PubMed] [Google Scholar]
• Sheng Z, Otani H, Brown MS, Goldstein JL. Independent regulation of sterol regulatory element-binding proteins 1 and 2 in hamster liver. Proc Natl Acad Sci U S A. 1995 Feb 14;92(4):935–938. [PMC free article] [PubMed] [Google Scholar]

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