Macrophages, microglial cells, and HLA-DR antigens in fetal and infant brain. (original) (raw)
- Journal List
- J Clin Pathol
- v.44(2); 1991 Feb
- PMC496969
J Clin Pathol. 1991 Feb; 44(2): 102–106.
Department of Neuropathology, Radcliffe Infirmary, Oxford.
Abstract
Immunohistochemical reactions for macrophages, microglia, and HLA-DR antigens were tested on frozen sections of necropsy brain tissue from 20 fetuses and infants ranging in age from 18 weeks' gestation to 8 months post term. No primary central nervous system disease was present but there were four cases of sudden infant death syndrome (SIDS). Macrophages were detected in all the samples studied and were located in the germinal matrix zone, in perivascular spaces throughout the brain, and in the leptomeninges and subependymal layer. Well differentiated microglia were present in all cases examined after 35 weeks' gestation and less well ramified forms were seen at earlier stages of gestation. HLA-DR antigens were detected on a small number of macrophages, chiefly in a perivascular location, in all but three cases. The fewest reactive cells and the weakest reactions occurred in the youngest fetuses. One case of SIDS showed increased foci of microglia in perivascular white matter: this case and one other case of SIDS were the only cases with well ramified microglia that expressed HLA-DR antigens. These findings may be relevant to an understanding of local immune responses in fetal brain infections, including human immunodeficiency virus infection.
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- Janossy G, Bofill M, Poulter LW, Rawlings E, Burford GD, Navarrete C, Ziegler A, Kelemen E. Separate ontogeny of two macrophage-like accessory cell populations in the human fetus. J Immunol. 1986 Jun 15;136(12):4354–4361. [PubMed] [Google Scholar]
- Murabe Y, Sano Y. Morphological studies on neuroglia. VI. Postnatal development of microglial cells. Cell Tissue Res. 1982;225(3):469–485. [PubMed] [Google Scholar]
- Hume DA, Perry VH, Gordon S. Immunohistochemical localization of a macrophage-specific antigen in developing mouse retina: phagocytosis of dying neurons and differentiation of microglial cells to form a regular array in the plexiform layers. J Cell Biol. 1983 Jul;97(1):253–257. [PMC free article] [PubMed] [Google Scholar]
- Perry VH, Hume DA, Gordon S. Immunohistochemical localization of macrophages and microglia in the adult and developing mouse brain. Neuroscience. 1985 Jun;15(2):313–326. [PubMed] [Google Scholar]
- Ling EA, Penney D, Leblond CP. Use of carbon labeling to demonstrate the role of blood monocytes as precursors of the 'ameboid cells' present in the corpus callosum of postnatal rats. J Comp Neurol. 1980 Oct 1;193(3):631–657. [PubMed] [Google Scholar]
- Woodroofe MN, Bellamy AS, Feldmann M, Davison AN, Cuzner ML. Immunocytochemical characterisation of the immune reaction in the central nervous system in multiple sclerosis. Possible role for microglia in lesion growth. J Neurol Sci. 1986 Jul;74(2-3):135–152. [PubMed] [Google Scholar]
- Boya J, Calvo J, Carbonell AL. Appearance of microglial cells in the postnatal rat retina. Arch Histol Jpn. 1987 May;50(2):223–228. [PubMed] [Google Scholar]
- Ashwell K. Development of microglia in the albino rabbit retina. J Comp Neurol. 1989 Sep 15;287(3):286–301. [PubMed] [Google Scholar]
- Kitamura T, Miyake T, Fujita S. Genesis of resting microglia in the gray matter of mouse hippocampus. J Comp Neurol. 1984 Jul 1;226(3):421–433. [PubMed] [Google Scholar]
- Schelper RL, Adrian EK., Jr Monocytes become macrophages; they do not become microglia: a light and electron microscopic autoradiographic study using 125-iododeoxyuridine. J Neuropathol Exp Neurol. 1986 Jan;45(1):1–19. [PubMed] [Google Scholar]
- Miles JM, Chou SM. A new immunoperoxidase marker for microglia in paraffin section. J Neuropathol Exp Neurol. 1988 Nov;47(6):579–587. [PubMed] [Google Scholar]
- Lent R, Linden R, Cavalcante LA. Transient populations of presumptive macrophages in the brain of the developing hamster, as indicated by endocytosis of blood-borne horseradish peroxidase. Neuroscience. 1985 Aug;15(4):1203–1215. [PubMed] [Google Scholar]
- Franklin WA, Mason DY, Pulford K, Falini B, Bliss E, Gatter KC, Stein H, Clarke LC, McGee JO. Immunohistological analysis of human mononuclear phagocytes and dendritic cells by using monoclonal antibodies. Lab Invest. 1986 Mar;54(3):322–335. [PubMed] [Google Scholar]
- Hayes GM, Woodroofe MN, Cuzner ML. Microglia are the major cell type expressing MHC class II in human white matter. J Neurol Sci. 1987 Aug;80(1):25–37. [PubMed] [Google Scholar]
- Esiri MM, Reading MC. Macrophage populations associated with multiple sclerosis plaques. Neuropathol Appl Neurobiol. 1987 Nov-Dec;13(6):451–465. [PubMed] [Google Scholar]
- Davey FR, Cordell JL, Erber WN, Pulford KA, Gatter KC, Mason DY. Monoclonal antibody (Y1/82A) with specificity towards peripheral blood monocytes and tissue macrophages. J Clin Pathol. 1988 Jul;41(7):753–758. [PMC free article] [PubMed] [Google Scholar]
- Robbins PA, Evans EL, Ding AH, Warner NL, Brodsky FM. Monoclonal antibodies that distinguish between class II antigens (HLA-DP, DQ, and DR) in 14 haplotypes. Hum Immunol. 1987 Apr;18(4):301–313. [PubMed] [Google Scholar]
- Herschkowitz N. Brain development in the fetus, neonate and infant. Biol Neonate. 1988;54(1):1–19. [PubMed] [Google Scholar]
- Mickel HS, Gilles FH. Changes in glial cells during human telencephalic myelinogenesis. Brain. 1970;93(2):337–346. [PubMed] [Google Scholar]
- Jellinger K, Seitelberger F, Kozik M. Perivascular accumulation of lipids in the infant human brain. Acta Neuropathol. 1971;19(4):331–342. [PubMed] [Google Scholar]
- BANKER BQ, LARROCHE JC. Periventricular leukomalacia of infancy. A form of neonatal anoxic encephalopathy. Arch Neurol. 1962 Nov;7:386–410. [PubMed] [Google Scholar]
- Leech RW, Alvord EC., Jr Anoxic-ischemic encephalopathy in the human neonatal period. The significance of brain stem involvement. Arch Neurol. 1977 Feb;34(2):109–113. [PubMed] [Google Scholar]
- Larroche JC, Amakawa H. Glia of myelination and fat deposit during early myelogenesis. Biol Neonate. 1973;22(5):421–435. [PubMed] [Google Scholar]
- Goddard-Finegold J, Sloper JJ, Esiri MM. Lipid-containing cells in brains of normal and hypoxic infant monkeys: a quantitative and ultrastructural study. Ann Neurol. 1989 Jul;26(1):34–40. [PubMed] [Google Scholar]
- Gadsdon DR, Emery JL. Fatty change in the brain in perinatal and unexpected death. Arch Dis Child. 1976 Jan;51(1):42–48. [PMC free article] [PubMed] [Google Scholar]
- Esiri MM, Urry P, Keeling J. Lipid-containing cells in the brain in sudden infant death syndrome. Dev Med Child Neurol. 1990 Apr;32(4):319–324. [PubMed] [Google Scholar]
- Daar AS, Fuggle SV, Fabre JW, Ting A, Morris PJ. The detailed distribution of MHC Class II antigens in normal human organs. Transplantation. 1984 Sep;38(3):293–298. [PubMed] [Google Scholar]
- Hauser SL, Bhan AK, Gilles FH, Hoban CJ, Reinherz EL, Schlossman SF, Weiner HL. Immunohistochemical staining of human brain with monoclonal antibodies that identify lymphocytes, monocytes, and the Ia antigen. J Neuroimmunol. 1983 Oct;5(2):197–205. [PubMed] [Google Scholar]
- Lampson LA, Hickey WF. Monoclonal antibody analysis of MHC expression in human brain biopsies: tissue ranging from "histologically normal" to that showing different levels of glial tumor involvement. J Immunol. 1986 Jun 1;136(11):4054–4062. [PubMed] [Google Scholar]
- Sobel RA, Ames MB. Major histocompatibility complex molecule expression in the human central nervous system: immunohistochemical analysis of 40 patients. J Neuropathol Exp Neurol. 1988 Jan;47(1):19–28. [PubMed] [Google Scholar]
- Nathan CF, Prendergast TJ, Wiebe ME, Stanley ER, Platzer E, Remold HG, Welte K, Rubin BY, Murray HW. Activation of human macrophages. Comparison of other cytokines with interferon-gamma. J Exp Med. 1984 Aug 1;160(2):600–605. [PMC free article] [PubMed] [Google Scholar]
- Mauerhoff T, Pujol-Borrell R, Mirakian R, Bottazzo GF. Differential expression and regulation of major histocompatibility complex (MHC) products in neural and glial cells of the human fetal brain. J Neuroimmunol. 1988 Jul;18(4):271–289. [PMC free article] [PubMed] [Google Scholar]
- Sharer LR, Epstein LG, Cho ES, Joshi VV, Meyenhofer MF, Rankin LF, Petito CK. Pathologic features of AIDS encephalopathy in children: evidence for LAV/HTLV-III infection of brain. Hum Pathol. 1986 Mar;17(3):271–284. [PubMed] [Google Scholar]
- Mannoji H, Yeger H, Becker LE. A specific histochemical marker (lectin Ricinus communis agglutinin-1) for normal human microglia, and application to routine histopathology. Acta Neuropathol. 1986;71(3-4):341–343. [PubMed] [Google Scholar]
- Adams CW, Poston RN, Buk SJ. Pathology, histochemistry and immunocytochemistry of lesions in acute multiple sclerosis. J Neurol Sci. 1989 Sep;92(2-3):291–306. [PubMed] [Google Scholar]
- Pulford KA, Rigney EM, Micklem KJ, Jones M, Stross WP, Gatter KC, Mason DY. KP1: a new monoclonal antibody that detects a monocyte/macrophage associated antigen in routinely processed tissue sections. J Clin Pathol. 1989 Apr;42(4):414–421. [PMC free article] [PubMed] [Google Scholar]
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