Proportionally more deleterious genetic variation in European than in African populations - PubMed (original) (raw)

. 2008 Feb 21;451(7181):994-7.

doi: 10.1038/nature06611.

Amit R Indap, Steffen Schmidt, Adam R Boyko, Ryan D Hernandez, Melissa J Hubisz, John J Sninsky, Thomas J White, Shamil R Sunyaev, Rasmus Nielsen, Andrew G Clark, Carlos D Bustamante

Affiliations

Proportionally more deleterious genetic variation in European than in African populations

Kirk E Lohmueller et al. Nature. 2008.

Abstract

Quantifying the number of deleterious mutations per diploid human genome is of crucial concern to both evolutionary and medical geneticists. Here we combine genome-wide polymorphism data from PCR-based exon resequencing, comparative genomic data across mammalian species, and protein structure predictions to estimate the number of functionally consequential single-nucleotide polymorphisms (SNPs) carried by each of 15 African American (AA) and 20 European American (EA) individuals. We find that AAs show significantly higher levels of nucleotide heterozygosity than do EAs for all categories of functional SNPs considered, including synonymous, non-synonymous, predicted 'benign', predicted 'possibly damaging' and predicted 'probably damaging' SNPs. This result is wholly consistent with previous work showing higher overall levels of nucleotide variation in African populations than in Europeans. EA individuals, in contrast, have significantly more genotypes homozygous for the derived allele at synonymous and non-synonymous SNPs and for the damaging allele at 'probably damaging' SNPs than AAs do. For SNPs segregating only in one population or the other, the proportion of non-synonymous SNPs is significantly higher in the EA sample (55.4%) than in the AA sample (47.0%; P < 2.3 x 10(-37)). We observe a similar proportional excess of SNPs that are inferred to be 'probably damaging' (15.9% in EA; 12.1% in AA; P < 3.3 x 10(-11)). Using extensive simulations, we show that this excess proportion of segregating damaging alleles in Europeans is probably a consequence of a bottleneck that Europeans experienced at about the time of the migration out of Africa.

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Figures

Figure 1

Figure 1. Distribution of the number of heterozygous and homozygous genotypes per individual

a, Number of heterozygous genotypes per individual at synonymous (S) or nonsynonymous (NS) SNPs. b, Number of genotypes homozygous for the derived allele per individual at synonymous (S) or nonsynonymous (NS) SNPs. c, Number of heterozygous genotypes per individual at possibly damaging (PO) or probably damaging (PR) SNPs. d, Number of genotypes homozygous for the damaging allele at possibly damaging (PO) or probably damaging (PR) SNPs. Dark horizontal lines within boxes indicate medians, and the whiskers indicate the ranges of the distributions. EA: European American; AA: African American.

Figure 2

Figure 2. Demography and selection can cause a proportional excess of nonsynonymous SNPs in Europeans

a,b Results of forward-simulations of a population that expanded (AA 2 in Supplementary Table 2), to represent the African American (AA) population and a population that experienced a bottleneck to represent the European (EA) population (EA 1 in Supplementary Table 2). a, Distribution of the proportion of nonsynonymous SNPs segregating in samples simulated under European (dashed curve) and African (solid curve) demographic models. Vertical lines show the observed proportions in the Applera dataset. b, Distribution of selection coefficients for simulated SNPs in the AA (white bars) and the EA (shaded bars) samples. The labels on the x-axis are the more negative limits of the bins. Error bars denote 95% intervals on the proportion of SNPs in each group. c–e, Expected distribution of SNPs over time during a population expansion (AA 2, solid lines), a long, mild bottleneck (EA 1, dashed lines), and a short, severe bottleneck (EA 6, dotted lines). Time moves forward in the figures from left to right. Solid vertical lines indicate when the populations changed size. Further details are given in Supplementary Table 2. c, The number of nonsynonymous SNPs, d, the number of synonymous SNPs and e, the proportion of nonsynonymous SNPs.

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