OKPALA JUDE CHINEDU | Ahmadu Bello University, Nigeria (original) (raw)

OKPALA JUDE CHINEDU

Phone: +2348068670184
Address: Department of Biochemistry, Ahmadu Bello University, Zaria, Kaduna State, Nigeria

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Research paper thumbnail of Effects of n-butanol fraction of Gongronema latifolium leave extract on some biochemical parameters in CCl 4 - induced oxidative damage in Wistar albino rats

Wister albino rats were divided into treatment group and LD 50 groups. Group A (normal control) w... more Wister albino rats were divided into treatment group and LD 50 groups. Group A (normal control) was given feed and water, Group B (vehicle control) was injected with olive oil intraperitoneally, while the rest groups (C, D, E, F and G) were injected intraperitoneally with a single dose of CCl 4 (148 mg/kg) as a 1:1 (v/v) solution in olive oil and all the animals were fasted for 36 h. This was repeated once every week for a period of four (4) weeks. At the end of 28 days of treatment, liver marker enzymes studies showed that there was significant (p<0.05) increase in the serum activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP)and bilirubin concentrations in CCl 4induced control group when compared with the normal control and induced treated groups but there was no significant (p>0.05) difference of these liver marker enzymes and bilirubin levels between the normal control and induced treated groups. Kidney function studies showed that there was significant (p<0.05) increase in creatinine and urea concentrations of CCl 4 -induced control group when compared with the normal control and induced treated groups but there was no significant (p>0.05) difference between the normal control and induced treated groups. Also, the kidney and liver antioxidant study revealed significant (p<0.05) decrease in superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) activities as well as an increase in MDA concentration in CCl 4 -induced control rats when compared with the normal control rats. Histological section of the organs shows that the levels of hepatic and renal damage were higher in CCl 4 -induction control rats when compared with the normal control and induced treated groups. These findings suggest that n-butanol fraction of methanolic leaves extract of G. latifolium may have anti-hepatotoxic, anti-nephrotoxic and antioxidative effects against CCl 4 -induced liver and kidney damage in albino rats.

Research paper thumbnail of Effects of n-butanol fraction of Gongronema latifolium leave extract on some biochemical parameters in CCl 4 - induced oxidative damage in Wistar albino rats

Wister albino rats were divided into treatment group and LD 50 groups. Group A (normal control) w... more Wister albino rats were divided into treatment group and LD 50 groups. Group A (normal control) was given feed and water, Group B (vehicle control) was injected with olive oil intraperitoneally, while the rest groups (C, D, E, F and G) were injected intraperitoneally with a single dose of CCl 4 (148 mg/kg) as a 1:1 (v/v) solution in olive oil and all the animals were fasted for 36 h. This was repeated once every week for a period of four (4) weeks. At the end of 28 days of treatment, liver marker enzymes studies showed that there was significant (p<0.05) increase in the serum activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP)and bilirubin concentrations in CCl 4induced control group when compared with the normal control and induced treated groups but there was no significant (p>0.05) difference of these liver marker enzymes and bilirubin levels between the normal control and induced treated groups. Kidney function studies showed that there was significant (p<0.05) increase in creatinine and urea concentrations of CCl 4 -induced control group when compared with the normal control and induced treated groups but there was no significant (p>0.05) difference between the normal control and induced treated groups. Also, the kidney and liver antioxidant study revealed significant (p<0.05) decrease in superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) activities as well as an increase in MDA concentration in CCl 4 -induced control rats when compared with the normal control rats. Histological section of the organs shows that the levels of hepatic and renal damage were higher in CCl 4 -induction control rats when compared with the normal control and induced treated groups. These findings suggest that n-butanol fraction of methanolic leaves extract of G. latifolium may have anti-hepatotoxic, anti-nephrotoxic and antioxidative effects against CCl 4 -induced liver and kidney damage in albino rats.

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