Fathy Osman | Agriculture Research Center, Egypt (original) (raw)

Papers by Fathy Osman

Research paper thumbnail of Microscopic and molecular detection of piroplasms among sheep in Upper Egypt

Frontiers in veterinary science, May 27, 2024

Conclusions: Taken together, this study confirms a high prevalence of piroplasmosis and o ers epi... more Conclusions: Taken together, this study confirms a high prevalence of piroplasmosis and o ers epidemiological and molecular insights into blood parasites in sheep from Upper Egypt, highlighting the importance of detecting these parasites in various hosts and their competent vectors (ticks).

Research paper thumbnail of Anti‐helminthic Resistance

Research paper thumbnail of Molecular identification of Haemonchus contortus in sheep from Upper Egypt

Frontiers in veterinary science, Feb 12, 2024

Introduction: Haemonchus spp. are considered the most important strongylid nematodes with a world... more Introduction: Haemonchus spp. are considered the most important strongylid nematodes with a worldwide distribution. The parasite's blood-sucking nature can lead to severe anemia in infected animals. Despite its widespread impact, there is a dearth of comprehensive data on morphological and molecular identification methods for Haemonchus spp. in sheep from Upper Egypt. To address this gap, our current study aimed to assess the prevalence of Haemonchus spp. in 400 sheep fecal samples. Methods: We employed microscopic examination and molecular techniques, using polymerase chain reaction (PCR) targeting the 18S gene for precise identification. Additionally, the potential risk factors associated with the infection by the parasite in sheep were explored. Results: The study pointed out that 33.00% (132 of 400) of the examined sheep were infected with Haemonchus spp. Sheep age and seasonal variability were found to be significant factors (p < 0.05) associated with the infection. Notably, sheep under 2 years old exhibited a higher risk, with an infection rate of 43.75% (84 out of 192), than their older counterparts. Furthermore, all reported infections were exclusively observed during the cold season, constituting 58.93% (132 out of 224) of cases. By contrast, no statistically significant association (p > 0.05) was found between the sex of the examined sheep and the occurrence of haemonchosis. Employing molecular methods, we isolated and identified the parasite through PCR analysis of cultured larvae, which were then subsequently confirmed as Haemonchus contortus via phylogenetic analysis. Discussion: The study concluded that there was a relatively high occurrence of H. contortus among sheep from Upper Egypt. We recommend the implementation of stringent and effective control measures to combat the infection and safeguard livestock health.

Research paper thumbnail of Effect of antioxidants on the volatiles of roasted sesame seeds

Journal of Agricultural and Food Chemistry, 1985

Nash, RG; Beall, ML, Jr. J. Agric. Food Chem. 1980,28,614. Nestrick, TJ; Lamparski, LL Anal. Chem... more Nash, RG; Beall, ML, Jr. J. Agric. Food Chem. 1980,28,614. Nestrick, TJ; Lamparski, LL Anal. Chem. 1982, 54, 2292. Quensen, JF; Matsumura, F. Enuiron. Toxicol. Chem. 1983,2, ... Sethunathan, N. Residue Rev. 1973,47, 143. Smith, A. E. Weed Sci. 1971,19, 536. Smith, ...

Research paper thumbnail of Clinico hematological and biochemical changes in camels affected with gastro-intestinal parasites

International Journal for Agro Veterinary and Medical Sciences, 2014

This study was conducted to determine the prevalence and effect of parasitic infection on clinica... more This study was conducted to determine the prevalence and effect of parasitic infection on clinical healthy, hematological and biochemical parameters in camels. Hundred fifty adult one humped camels of both sex were examined for helminthes parasite according to the standard procedures. Examination of fecal samples from camels shows 63 (41 %) were harboring different helminthes nematode species (36.15%) ,where coproculture were recorded.Trichostrongylus spp,,Nematodirus spp,Osterotagia spp and Osphegestomum spp Trichuris spp. Protozoon parasites, 9.23% (Eimeria spp) nd cestod parasites,3.07% (Monezia spp), while blood smear examination for excluded the camels infected by blood parasites. Single infection recorded in (n=58,44.61%) and mixed infection recorded in (n=5,3.84%). Packed cell volume (PCV), hemoglobin concentration (HB), Total WBC count and red blood cell counts were significantly (P < 0.01) affected in the infected camels compared to the control healthy camels. Parasite infection in camels leads to macrocytic anemia. Biochemical analysis revealed that significant affected of AST,ALT and BUN in the infected camels compared to the control healthy camel,so we can concluded that Camels were affected by gastrointestinal parasite exhibited different clinical signs,a significant change in hematological and biochemical values between the infected and control camels.

Research paper thumbnail of Prevalence of helminthes of sheep in Assiut Governorate, Egypt

Assiut Veterinary Medical Journal

Research paper thumbnail of Serological, Biochemical, and Epidemiological Studies on Sarcocystis in Sheep and Goats in Assuit Governorate. Egypt

Research paper thumbnail of Evaluation of the concentrations of products of nitric oxide oxidation and some antioxidant vitamins in sheep naturally infested with parasites

International Journal for Agro Veterinary and Medical Sciences, 2012

The aim of the present study was to determine serum concentrations of the products of nitric oxid... more The aim of the present study was to determine serum concentrations of the products of nitric oxide oxidation (nitrate and nitrite) and some vitamins (retinol acetate, αand δ-tocopherol, and vitamin D 3) in sheep naturally infected with Babesia ovis. The investigation included 30 infected and 10 control sheep. Serum α-tocopherol levels were significantly lower (P < 0.05), and nitrate and nitrite concentrations were significantly higher (P < 0.05) in infected animals than in controls. It is thought that the elevated nitrate and nitrite levels of the sheep infected with babesiosis were due to the result of damage caused by Babesia. On the other hand, a significant decrease was observed in α-tocopherol levels in sheep with babesiosis because of damage and pathophysiological changes to erythrocytes.

Research paper thumbnail of Clinico- hematological and biochemical changes in camels infected with gastro-intestinal parasites

Journal of Animal Science Advances, 2015

Research paper thumbnail of Evaluation of Antioxidant Response Mechanism in Fattening Cattle Calves Suffering from Babesiosis in New-Valley- Governorate. Egypt

Journal of Veterinary Advances, 2013

Research paper thumbnail of Some studies on prevalence and effect of Thieleria infection on erythrocytes profile in camel in some localities at New-Valley, Governorate, Egypt

Journal of Animal Science Advances, 2015

Research paper thumbnail of The prevalence and biochemical characters of hydatid cyst in sheep and goats slaughtered at El-Karhga, New-Valley Governorate, Egypt

International Journal for Agro Veterinary and Medical Sciences, 2014

A total of 987 carcasses of sheep (459) and goats (528) of different ages, in the period of June,... more A total of 987 carcasses of sheep (459) and goats (528) of different ages, in the period of June, 2012-June, 2013, reared in different localities at El-Kharga city (in and out the abattoir), where 37 (8.06%) of sheep and 29 (5.49%) of goats were found harbored hydatid cyst liver, and lung were the most infected organ in sheep at rate of 4.57% and 2.61%, respectively, but in goats the lung was the most predilection site than liver in rate of 3.03% and 2.46% respectively, both liver and lung were cystic, in sheep only in rate of 0.65%, and the under develop cyst recorded in kidney of sheep 0.28%. Sheep fell in age category of 3 year and above were more infected (15.29%) compared to 1 to 3 year (4.23%) and below 1 year (0%) old. Likewise, the prevalence in goats was 7.89%, 3.79% and 0% respectively in the same age groups. Fertility of cyst was highest in sheep than goats in rate of 62.16% and 58.62%, respectively. The biochemical analysis revealed significant increase in protein level of lung cysts extract (P < 0.01) than liver cyst extract in sheep and goats. While liver cyst extract revealed significant increase in both of, creatinine (P < 0.01), calcium ions (P < 0.01) and magnesium ions (P < 0.05) than lung cyst extract in both sheep and goats, while cholesterol and triglyceride revealed significant increase in liver cyst extract of sheep and no change in liver cyst extract of goats. The differences in chemical component values of cyst extract in both sheep and goat and cystic organ in the same animal may pointed some strain variability in parasite metabolism, growth rate or even strain variation.

Research paper thumbnail of Comparative Study Between Serological Analysis and Visual Meat Inspection for Hydatid Cyst in Camels

Received at: 28/1/2014 Accepted: 24/2/2014 The present investigation was conducted (during PM exa... more Received at: 28/1/2014 Accepted: 24/2/2014 The present investigation was conducted (during PM examination of the slaughtered carcasses) to evaluate the sensitivity and specificity of Enzyme-linked immunosorbent assay (ELISA) in identifying camels infected with hydatid cysts before slaughtering and to assess the prevalence of hydatidosis in camels sacrificed in Assuit Governorate. Hydatid cyst count and characterization were conducted based on routine meat inspection. Slaughterhouse samples of 200 camels were collected through weekly visits. Hydatid cysts in livers, lungs and kidneys were detected and counted, also the fertility rate of the cysts was examined. Out of these, 12 (6%) were found to harbor hydatid cyst, in livers 9(75%), lungs 2(17%) and kidney 1(8%).On the other side, fertile cysts 5(41.7%) were found more frequently in livers 4(33.3%) than in lungs 1(8.3%), while sterile cysts7(58.3%) found in livers , lungs and kidneys 5(41.7% ), 1(8.1) and 1(8.3%) respectively. In ad...

Research paper thumbnail of Studies on sarcocystis in sheep and goats using serological survey in New Valley governorate. Egypt

IJAVMS, 2014

The aim of the present study was to provide an epidemiologic investigation of the seroprevalence ... more The aim of the present study was to provide an epidemiologic investigation of the seroprevalence of antibodies to sarcocystis in the sheep and goat population in New-Valley Governorate (Egypt). Where sarcocystis is one of the important disease agent of sheep and goats and is known to be transmitted to humans. By means of the enzyme –linked immunosorbant assay (ELIZA), blood serum antibody of sarcocystis were analyzed in 330 sheep and 210 goats originated from 9 settlements in New-Valley Governorate,where in sheep215 samples(65.15%) were positive while in goats the seropositive samples were 108 (51.4%), this percentage differed according to the region. The highest percentage appeared in EL-Dakla,EL-Farafra and EL-Kharga on the fallow in both sheep and goats while in relation to sex the highest percentage was in females than males but in relation to age, we found the highest percentage in adult than young in both sheep and goats. Finally, we can conclude that ELIZA test is an efficient diagnostic test for sarcocystis and also we can say the high seroprevalence of sarcocystis in sheep and goats indicated the importance of sarcocystis in public health through the consumption of undercooked meat of these animals. Microscopic examination revealed high percentage of infection when compared by ELIZA test as infection in sheep was totally(88%) while in goats arrived in (75%).

Research paper thumbnail of Microscopic and molecular detection of piroplasms among sheep in Upper Egypt

Frontiers in veterinary science, May 27, 2024

Conclusions: Taken together, this study confirms a high prevalence of piroplasmosis and o ers epi... more Conclusions: Taken together, this study confirms a high prevalence of piroplasmosis and o ers epidemiological and molecular insights into blood parasites in sheep from Upper Egypt, highlighting the importance of detecting these parasites in various hosts and their competent vectors (ticks).

Research paper thumbnail of Anti‐helminthic Resistance

Research paper thumbnail of Molecular identification of Haemonchus contortus in sheep from Upper Egypt

Frontiers in veterinary science, Feb 12, 2024

Introduction: Haemonchus spp. are considered the most important strongylid nematodes with a world... more Introduction: Haemonchus spp. are considered the most important strongylid nematodes with a worldwide distribution. The parasite's blood-sucking nature can lead to severe anemia in infected animals. Despite its widespread impact, there is a dearth of comprehensive data on morphological and molecular identification methods for Haemonchus spp. in sheep from Upper Egypt. To address this gap, our current study aimed to assess the prevalence of Haemonchus spp. in 400 sheep fecal samples. Methods: We employed microscopic examination and molecular techniques, using polymerase chain reaction (PCR) targeting the 18S gene for precise identification. Additionally, the potential risk factors associated with the infection by the parasite in sheep were explored. Results: The study pointed out that 33.00% (132 of 400) of the examined sheep were infected with Haemonchus spp. Sheep age and seasonal variability were found to be significant factors (p < 0.05) associated with the infection. Notably, sheep under 2 years old exhibited a higher risk, with an infection rate of 43.75% (84 out of 192), than their older counterparts. Furthermore, all reported infections were exclusively observed during the cold season, constituting 58.93% (132 out of 224) of cases. By contrast, no statistically significant association (p > 0.05) was found between the sex of the examined sheep and the occurrence of haemonchosis. Employing molecular methods, we isolated and identified the parasite through PCR analysis of cultured larvae, which were then subsequently confirmed as Haemonchus contortus via phylogenetic analysis. Discussion: The study concluded that there was a relatively high occurrence of H. contortus among sheep from Upper Egypt. We recommend the implementation of stringent and effective control measures to combat the infection and safeguard livestock health.

Research paper thumbnail of Effect of antioxidants on the volatiles of roasted sesame seeds

Journal of Agricultural and Food Chemistry, 1985

Nash, RG; Beall, ML, Jr. J. Agric. Food Chem. 1980,28,614. Nestrick, TJ; Lamparski, LL Anal. Chem... more Nash, RG; Beall, ML, Jr. J. Agric. Food Chem. 1980,28,614. Nestrick, TJ; Lamparski, LL Anal. Chem. 1982, 54, 2292. Quensen, JF; Matsumura, F. Enuiron. Toxicol. Chem. 1983,2, ... Sethunathan, N. Residue Rev. 1973,47, 143. Smith, A. E. Weed Sci. 1971,19, 536. Smith, ...

Research paper thumbnail of Clinico hematological and biochemical changes in camels affected with gastro-intestinal parasites

International Journal for Agro Veterinary and Medical Sciences, 2014

This study was conducted to determine the prevalence and effect of parasitic infection on clinica... more This study was conducted to determine the prevalence and effect of parasitic infection on clinical healthy, hematological and biochemical parameters in camels. Hundred fifty adult one humped camels of both sex were examined for helminthes parasite according to the standard procedures. Examination of fecal samples from camels shows 63 (41 %) were harboring different helminthes nematode species (36.15%) ,where coproculture were recorded.Trichostrongylus spp,,Nematodirus spp,Osterotagia spp and Osphegestomum spp Trichuris spp. Protozoon parasites, 9.23% (Eimeria spp) nd cestod parasites,3.07% (Monezia spp), while blood smear examination for excluded the camels infected by blood parasites. Single infection recorded in (n=58,44.61%) and mixed infection recorded in (n=5,3.84%). Packed cell volume (PCV), hemoglobin concentration (HB), Total WBC count and red blood cell counts were significantly (P < 0.01) affected in the infected camels compared to the control healthy camels. Parasite infection in camels leads to macrocytic anemia. Biochemical analysis revealed that significant affected of AST,ALT and BUN in the infected camels compared to the control healthy camel,so we can concluded that Camels were affected by gastrointestinal parasite exhibited different clinical signs,a significant change in hematological and biochemical values between the infected and control camels.

Research paper thumbnail of Prevalence of helminthes of sheep in Assiut Governorate, Egypt

Assiut Veterinary Medical Journal

Research paper thumbnail of Serological, Biochemical, and Epidemiological Studies on Sarcocystis in Sheep and Goats in Assuit Governorate. Egypt

Research paper thumbnail of Evaluation of the concentrations of products of nitric oxide oxidation and some antioxidant vitamins in sheep naturally infested with parasites

International Journal for Agro Veterinary and Medical Sciences, 2012

The aim of the present study was to determine serum concentrations of the products of nitric oxid... more The aim of the present study was to determine serum concentrations of the products of nitric oxide oxidation (nitrate and nitrite) and some vitamins (retinol acetate, αand δ-tocopherol, and vitamin D 3) in sheep naturally infected with Babesia ovis. The investigation included 30 infected and 10 control sheep. Serum α-tocopherol levels were significantly lower (P < 0.05), and nitrate and nitrite concentrations were significantly higher (P < 0.05) in infected animals than in controls. It is thought that the elevated nitrate and nitrite levels of the sheep infected with babesiosis were due to the result of damage caused by Babesia. On the other hand, a significant decrease was observed in α-tocopherol levels in sheep with babesiosis because of damage and pathophysiological changes to erythrocytes.

Research paper thumbnail of Clinico- hematological and biochemical changes in camels infected with gastro-intestinal parasites

Journal of Animal Science Advances, 2015

Research paper thumbnail of Evaluation of Antioxidant Response Mechanism in Fattening Cattle Calves Suffering from Babesiosis in New-Valley- Governorate. Egypt

Journal of Veterinary Advances, 2013

Research paper thumbnail of Some studies on prevalence and effect of Thieleria infection on erythrocytes profile in camel in some localities at New-Valley, Governorate, Egypt

Journal of Animal Science Advances, 2015

Research paper thumbnail of The prevalence and biochemical characters of hydatid cyst in sheep and goats slaughtered at El-Karhga, New-Valley Governorate, Egypt

International Journal for Agro Veterinary and Medical Sciences, 2014

A total of 987 carcasses of sheep (459) and goats (528) of different ages, in the period of June,... more A total of 987 carcasses of sheep (459) and goats (528) of different ages, in the period of June, 2012-June, 2013, reared in different localities at El-Kharga city (in and out the abattoir), where 37 (8.06%) of sheep and 29 (5.49%) of goats were found harbored hydatid cyst liver, and lung were the most infected organ in sheep at rate of 4.57% and 2.61%, respectively, but in goats the lung was the most predilection site than liver in rate of 3.03% and 2.46% respectively, both liver and lung were cystic, in sheep only in rate of 0.65%, and the under develop cyst recorded in kidney of sheep 0.28%. Sheep fell in age category of 3 year and above were more infected (15.29%) compared to 1 to 3 year (4.23%) and below 1 year (0%) old. Likewise, the prevalence in goats was 7.89%, 3.79% and 0% respectively in the same age groups. Fertility of cyst was highest in sheep than goats in rate of 62.16% and 58.62%, respectively. The biochemical analysis revealed significant increase in protein level of lung cysts extract (P < 0.01) than liver cyst extract in sheep and goats. While liver cyst extract revealed significant increase in both of, creatinine (P < 0.01), calcium ions (P < 0.01) and magnesium ions (P < 0.05) than lung cyst extract in both sheep and goats, while cholesterol and triglyceride revealed significant increase in liver cyst extract of sheep and no change in liver cyst extract of goats. The differences in chemical component values of cyst extract in both sheep and goat and cystic organ in the same animal may pointed some strain variability in parasite metabolism, growth rate or even strain variation.

Research paper thumbnail of Comparative Study Between Serological Analysis and Visual Meat Inspection for Hydatid Cyst in Camels

Received at: 28/1/2014 Accepted: 24/2/2014 The present investigation was conducted (during PM exa... more Received at: 28/1/2014 Accepted: 24/2/2014 The present investigation was conducted (during PM examination of the slaughtered carcasses) to evaluate the sensitivity and specificity of Enzyme-linked immunosorbent assay (ELISA) in identifying camels infected with hydatid cysts before slaughtering and to assess the prevalence of hydatidosis in camels sacrificed in Assuit Governorate. Hydatid cyst count and characterization were conducted based on routine meat inspection. Slaughterhouse samples of 200 camels were collected through weekly visits. Hydatid cysts in livers, lungs and kidneys were detected and counted, also the fertility rate of the cysts was examined. Out of these, 12 (6%) were found to harbor hydatid cyst, in livers 9(75%), lungs 2(17%) and kidney 1(8%).On the other side, fertile cysts 5(41.7%) were found more frequently in livers 4(33.3%) than in lungs 1(8.3%), while sterile cysts7(58.3%) found in livers , lungs and kidneys 5(41.7% ), 1(8.1) and 1(8.3%) respectively. In ad...

Research paper thumbnail of Studies on sarcocystis in sheep and goats using serological survey in New Valley governorate. Egypt

IJAVMS, 2014

The aim of the present study was to provide an epidemiologic investigation of the seroprevalence ... more The aim of the present study was to provide an epidemiologic investigation of the seroprevalence of antibodies to sarcocystis in the sheep and goat population in New-Valley Governorate (Egypt). Where sarcocystis is one of the important disease agent of sheep and goats and is known to be transmitted to humans. By means of the enzyme –linked immunosorbant assay (ELIZA), blood serum antibody of sarcocystis were analyzed in 330 sheep and 210 goats originated from 9 settlements in New-Valley Governorate,where in sheep215 samples(65.15%) were positive while in goats the seropositive samples were 108 (51.4%), this percentage differed according to the region. The highest percentage appeared in EL-Dakla,EL-Farafra and EL-Kharga on the fallow in both sheep and goats while in relation to sex the highest percentage was in females than males but in relation to age, we found the highest percentage in adult than young in both sheep and goats. Finally, we can conclude that ELIZA test is an efficient diagnostic test for sarcocystis and also we can say the high seroprevalence of sarcocystis in sheep and goats indicated the importance of sarcocystis in public health through the consumption of undercooked meat of these animals. Microscopic examination revealed high percentage of infection when compared by ELIZA test as infection in sheep was totally(88%) while in goats arrived in (75%).