Dr.Nahi Y Yaseen | AL-mustanseriyah university (original) (raw)
Papers by Dr.Nahi Y Yaseen
Veterinary and Animal Science, Aug 1, 2021
Highlights • This study includes the first report on the isolation of the BLV virus in Iraq ever.... more Highlights • This study includes the first report on the isolation of the BLV virus in Iraq ever.• Cattle infected with PL can only be the source for viral isolation.• Cytopathological features of the virus infection are differ depending on the cell type.• The virus can be propagated in different cell types including human cells.• This study describes first cellular and genotyping characterization for BLV virus Iraqi strain.
Journal of Chemical Neuroanatomy, Nov 1, 2016
Efficient differentiation of BM-MSCs into motor neuron-like cells revised protocol Differentiat... more Efficient differentiation of BM-MSCs into motor neuron-like cells revised protocol Differentiation confirmed using light, electron microscope and Immunocytochemistry Cells were function as motor neuron as assessed by measuring acetylcholine by HPLC Motor neuron axon elongated by nerve growth factor Using a 3D collagen matrix showed that cells were induced into motor neuron
Stem Cell Discovery, 2016
In the brain, there are hundreds of types of specialized neurons and to generate one type of them... more In the brain, there are hundreds of types of specialized neurons and to generate one type of them we need to have neural progenitors for differentiation to specific neuron type. Mesenchymal stem cells (MSCs) are easily isolated, cultured, manipulated ex vivo, showing great potential for therapeutic applications. The adult MSCs have the potential to produce progeny that differentiate into a variety of cell types such as neurons. This fact suggests that MSCs derived neurons are an important cell type and a deep understanding of the molecular characteristics of it would significantly enhance the advancement of cell therapy for neurological disorders. Therefore, in this study, we isolated, identified, and studied neural progenitors by measuring expression levels through neurogenesis pathway of three neural differentiation markers nestin (NES), neurofilament (NF-L), and microtubule association protein (MAP-2) from mouse bone marrow MSCs (mouse bmMSCs) by using butylated hydroxyanisole (BHA) and diethyl sulfoxide (DMSO) as neural inducers agents. The results of immunocytochemistry and Real Time-PCR showed that in contrast to MSCs, neural differentiated cells showed neural progenitor pattern by showing stable increase in NES gene expression through differentiation process with increasing the protein expression through different exposures times, while NF-L gene and protein expression start to increased after 48 h but not replaced the NES expression completely even when its expression passed NES levels. The maturation marker Map-2 expression was low during the duration of differentiation period in protein and gene expression, which prove that these cells are still progenitors and can be redirected into specific type of neurons by further treatments.
EJC Supplements, 2010
ABSTRACT The avian paramyxovirus, Newcastle disease virus (NDV), is considered to be very promisi... more ABSTRACT The avian paramyxovirus, Newcastle disease virus (NDV), is considered to be very promising. Since cytolytic strains of NDV possess a number of desirable properties in this regard. To evaluate the effectiveness of NDV Iraqi isolate (NDV-Iraqi Ahmed Nahi – IAN) as a tumor cytolytic agent, we have performed in vitro and in vivo experiments. In vitro tests studied oncolytic activity on different tumor cell lines by light and electron microscope. In vivo experiment using murine mammary adenocarcinoma allograft grown in mice. We compared antitumor activity of intratumoral injection of NDV-IAN to systemic intraperitonial treatment. In vitro results revealed necrosis and apoptosis induction. While in vivo results showed intratumoral treatment caused average of 92&percnt; growth inhibition (p < 0.0001), while intraperitonial treatment show 79&percnt; growth inhibition at the end of the experiment (p < 0.0001) compared to control group. Furthermore treatment groups showed prolong surviving. Histopathological pictures showed massive area of necrosis with infiltration of inflammatory cells mainly lymphocyte. Ultrastructural study showed budding of the virus from the treated tumor cells. Our results suggest that NDV Iraqi isolate (NDV-IAN) as a promising antitumor agent.
BULGARIAN JOURNAL OF VETERINARY MEDICINE, 2022
Cancer cells heavily utilise angiogenesis process to increase vascularisation for tumour mass gro... more Cancer cells heavily utilise angiogenesis process to increase vascularisation for tumour mass growth and spread, so targeting this process is important to create an effective therapy. The AMHA1 strain of Newcastle disease virus (NDV) is an RNA virus with natural oncotropism. NDV induces direct tumour cytolysis, apoptosis, and immune stimulation. This work aimed to test NDV anti-angiogenic activity in a breast cancer model. To evaluate NDV’s antitumour effect in vivo, NDV was tested against mammary adenocarcinoma AN3 transplanted in syngeneic immunocompetent mice. In vivo antiangiogenic activity was evaluated by quantifying the blood vessels in treated and control tumour sections. In vitro experiments that exposed AMN3 mammary adenocarcinoma cells and Hep-2 laryngeal carcinoma cells to NDV at different time intervals were performed to identify the exact mechanism of anti-angiogenesis by using angiogenesis microarray slides. In vivo results showed significant tumour regression and sig...
Iraqi Journal of Cancer and Medical Genetics, 2014
T main objective of this research to study carcinogenic effects of 17β-estradiol in mice by study... more T main objective of this research to study carcinogenic effects of 17β-estradiol in mice by studying on haematological parameters (Hb, PCV, RBC, WBC and DWBC), mitotic index and histopathological changes of ovary, uterus and mammary gland. The following results which obtained from the current research: The effects of 17β-estradiol for six months on blood picture had no-significant differences on the blood haemoglobin (Hb) (11.55 and 11.69 gm/dl), total number of red blood cells (RBC) counts in the peripheral blood. RBC counts were 7.48 and 6.98 × 106/mm3 for the two groups respectively. Basophiles percent (3.40 and 3.54%) also (8.68 and 8.09%) for Monocytes percent for the two experimental groups respectively. Significant decrease (P˂0.05) in PCV% were noticed between the control and treated group (37.40and 31.28 %) respectively, and Lymphocyte percent (49.00 and 46.03%). Also significant increases (P˂0.05) were observed in the WBC count between the control and the second group (7.0...
Journal of Al-Nahrain University-Science, 2016
Motor neuron cell is responsible for transfer neural instruct from brain to peripheral muscle thr... more Motor neuron cell is responsible for transfer neural instruct from brain to peripheral muscle through spinal cord; therefore any defect in these cells or spinal cord will affect motion. This study was designed to induce differentiation of Bone marrow mesenchymal stem cells (BM-MSCs) into neuron cells. BM-MSCs were isolated from bone stroma of femur and tibia of albino male mice and tested immunocytochemically for CD44, CD90, and CD105 expression and showed positive staining, while showed negative staining for CD34. Differentiation of BM-MSCs to motor neuron involved two main steps. Firs; induction of BM-MSCs by addition of 1mM mercaptoethanol (BME) in fetal bovine serum (FBS) in minimum essential medium MEM for 24 h and 2mM BME in free serum media for 2h. In the second step of induction retinoic acid, sonic hedgehog and nerve growth factor were added in free serum MEM for 4 days. Results revealed that the differentiation medium used was very efficient in directing the BM-MSCs to motor neural cell and showed positive reactivity to specific motor neural markers that used for detection of motor neuron cells like microtubule associated protein-2 antibodies and acetylcholine transferase antibody.
In Vitro Cellular & Developmental Biology - Animal, 1991
Iraqi Journal of Cancer and Medical Genetics, 2012
Asociación Española de Estudios Anglo-Norteamericanos. AEDEAN, 2020
The Iraqi Journal of Veterinary Medicine, 2010
The in vivo study was designed to evaluate the anticancer effects of these three types of the ext... more The in vivo study was designed to evaluate the anticancer effects of these three types of the extracts on the AM-3 (Murine mammary adenocarcinoma). The LD50 was (5 g/kg, 6 g/kg and 8 g/kg) for ethanolic, hot aqueous and cold aqueous extracts respectively. The therapeutic doses of extracts were daily injected subcutaneously for two weeks, three weeks and four weeks. The histopathological study revealed pathological changes in some organs; the group that treated with ethanolic extract showed sever deposition of amyloid in some organs like spleen. The blood vessel filled with inflammatory cells as neutrophiles and cells infiltration in the lung tissue. Hot and cold aqueous extracts showed inflammatory mononuclear cells infiltration in the kidney and lung, section with necrosis in most of the cancer cells in the lung tissue; with few necrotic changes in the normal tissue were noticed after one month of treatment by hot and cold aqueous extracts.
arXiv: Biological Physics, Dec 1, 2015
Conventional kinesin walks by a handover hand mechanism on the microtubule (MT) by taking ∼ 8nm d... more Conventional kinesin walks by a handover hand mechanism on the microtubule (MT) by taking ∼ 8nm discrete steps, and consumes one ATP molecule per step. The time needed to complete a single step is on the order of twenty microseconds. We show, using simulations of a coarse-grained model of the complex containing the two motor heads, the MT, and the coiled coil that in order to obtain quantitative agreement with experiments for the stepping kinetics hydrodynamic interactions (HI) have to be included. In simulations without hydrodynamic interactions spanning nearly twenty µs not a single step was completed in hundred trajectories. In sharp contrast, nearly 14% of the steps reached the target binding site within 6µs when HI were included. Somewhat surprisingly, there are qualitative differences in the diffusion pathways in simulations with and without HI. The extent of movement of the trailing head of kinesin on the MT during the diffusion stage of stepping is considerably greater in simulations with HI than in those without HI. Our results suggest that inclusion of HI is crucial in the accurate description of motility of other motors as well.
Iraqi Journal of Cancer and Medical Genetics, 2014
Iraqi Journal of Cancer and Medical Genetics, 2015
R egional immune response with mammary gland carcinoma was studied statistically. However the pro... more R egional immune response with mammary gland carcinoma was studied statistically. However the prognostic value remains conflicting. This study was conducted on thirty normal mice which were inoculated with mammary gland carcinoma. The tumour size of the animals under study were measured before and after laser irradiation by using a vernier and compared with that of non irradiated animals with laser (control group). The aim of this study was to evaluate the effect of low level laser (LLL) on increasing the response of immune system by stimulating the spleen action to inhibit the cancer cell activity and then decrease the tumour size of inoculated mice. The results of the gross observation showed enlargement in the irradiated spleen in comparison with that of non irradiated ones. It could be said that the enlargement which occurred in the spleen proved that there was an increase in immune response by using laser stimulation. From this study, it may concluded that low level laser(LLL) ...
The Iraqi Journal of Veterinary Medicine, Jun 30, 2010
In order to investigate the phytochemicals presence in the seeds of local plant of Datura stramon... more In order to investigate the phytochemicals presence in the seeds of local plant of Datura stramonium rather than alkaloids. For this purpose two types of Datura stramonium extract were prepared. The first was precipitated by acetone and the other was destructed by ether then precipitated with ammonium sulphate. The first extract yielded 2.5gram/ 5 gram of seeds weight; while, the second extract yielded 1.23 gram/ 5 gram of seed weight. Biochemical tests exhibited that lectin and trace amounts of glycosides were presented in the acetone precipitated extract. Whereas, the ether extract contained lectin, tannin, flavonoids, and trace amounts of alkaloids.
Veterinary and Animal Science, Aug 1, 2021
Highlights • This study includes the first report on the isolation of the BLV virus in Iraq ever.... more Highlights • This study includes the first report on the isolation of the BLV virus in Iraq ever.• Cattle infected with PL can only be the source for viral isolation.• Cytopathological features of the virus infection are differ depending on the cell type.• The virus can be propagated in different cell types including human cells.• This study describes first cellular and genotyping characterization for BLV virus Iraqi strain.
Journal of Chemical Neuroanatomy, Nov 1, 2016
Efficient differentiation of BM-MSCs into motor neuron-like cells revised protocol Differentiat... more Efficient differentiation of BM-MSCs into motor neuron-like cells revised protocol Differentiation confirmed using light, electron microscope and Immunocytochemistry Cells were function as motor neuron as assessed by measuring acetylcholine by HPLC Motor neuron axon elongated by nerve growth factor Using a 3D collagen matrix showed that cells were induced into motor neuron
Stem Cell Discovery, 2016
In the brain, there are hundreds of types of specialized neurons and to generate one type of them... more In the brain, there are hundreds of types of specialized neurons and to generate one type of them we need to have neural progenitors for differentiation to specific neuron type. Mesenchymal stem cells (MSCs) are easily isolated, cultured, manipulated ex vivo, showing great potential for therapeutic applications. The adult MSCs have the potential to produce progeny that differentiate into a variety of cell types such as neurons. This fact suggests that MSCs derived neurons are an important cell type and a deep understanding of the molecular characteristics of it would significantly enhance the advancement of cell therapy for neurological disorders. Therefore, in this study, we isolated, identified, and studied neural progenitors by measuring expression levels through neurogenesis pathway of three neural differentiation markers nestin (NES), neurofilament (NF-L), and microtubule association protein (MAP-2) from mouse bone marrow MSCs (mouse bmMSCs) by using butylated hydroxyanisole (BHA) and diethyl sulfoxide (DMSO) as neural inducers agents. The results of immunocytochemistry and Real Time-PCR showed that in contrast to MSCs, neural differentiated cells showed neural progenitor pattern by showing stable increase in NES gene expression through differentiation process with increasing the protein expression through different exposures times, while NF-L gene and protein expression start to increased after 48 h but not replaced the NES expression completely even when its expression passed NES levels. The maturation marker Map-2 expression was low during the duration of differentiation period in protein and gene expression, which prove that these cells are still progenitors and can be redirected into specific type of neurons by further treatments.
EJC Supplements, 2010
ABSTRACT The avian paramyxovirus, Newcastle disease virus (NDV), is considered to be very promisi... more ABSTRACT The avian paramyxovirus, Newcastle disease virus (NDV), is considered to be very promising. Since cytolytic strains of NDV possess a number of desirable properties in this regard. To evaluate the effectiveness of NDV Iraqi isolate (NDV-Iraqi Ahmed Nahi – IAN) as a tumor cytolytic agent, we have performed in vitro and in vivo experiments. In vitro tests studied oncolytic activity on different tumor cell lines by light and electron microscope. In vivo experiment using murine mammary adenocarcinoma allograft grown in mice. We compared antitumor activity of intratumoral injection of NDV-IAN to systemic intraperitonial treatment. In vitro results revealed necrosis and apoptosis induction. While in vivo results showed intratumoral treatment caused average of 92&percnt; growth inhibition (p < 0.0001), while intraperitonial treatment show 79&percnt; growth inhibition at the end of the experiment (p < 0.0001) compared to control group. Furthermore treatment groups showed prolong surviving. Histopathological pictures showed massive area of necrosis with infiltration of inflammatory cells mainly lymphocyte. Ultrastructural study showed budding of the virus from the treated tumor cells. Our results suggest that NDV Iraqi isolate (NDV-IAN) as a promising antitumor agent.
BULGARIAN JOURNAL OF VETERINARY MEDICINE, 2022
Cancer cells heavily utilise angiogenesis process to increase vascularisation for tumour mass gro... more Cancer cells heavily utilise angiogenesis process to increase vascularisation for tumour mass growth and spread, so targeting this process is important to create an effective therapy. The AMHA1 strain of Newcastle disease virus (NDV) is an RNA virus with natural oncotropism. NDV induces direct tumour cytolysis, apoptosis, and immune stimulation. This work aimed to test NDV anti-angiogenic activity in a breast cancer model. To evaluate NDV’s antitumour effect in vivo, NDV was tested against mammary adenocarcinoma AN3 transplanted in syngeneic immunocompetent mice. In vivo antiangiogenic activity was evaluated by quantifying the blood vessels in treated and control tumour sections. In vitro experiments that exposed AMN3 mammary adenocarcinoma cells and Hep-2 laryngeal carcinoma cells to NDV at different time intervals were performed to identify the exact mechanism of anti-angiogenesis by using angiogenesis microarray slides. In vivo results showed significant tumour regression and sig...
Iraqi Journal of Cancer and Medical Genetics, 2014
T main objective of this research to study carcinogenic effects of 17β-estradiol in mice by study... more T main objective of this research to study carcinogenic effects of 17β-estradiol in mice by studying on haematological parameters (Hb, PCV, RBC, WBC and DWBC), mitotic index and histopathological changes of ovary, uterus and mammary gland. The following results which obtained from the current research: The effects of 17β-estradiol for six months on blood picture had no-significant differences on the blood haemoglobin (Hb) (11.55 and 11.69 gm/dl), total number of red blood cells (RBC) counts in the peripheral blood. RBC counts were 7.48 and 6.98 × 106/mm3 for the two groups respectively. Basophiles percent (3.40 and 3.54%) also (8.68 and 8.09%) for Monocytes percent for the two experimental groups respectively. Significant decrease (P˂0.05) in PCV% were noticed between the control and treated group (37.40and 31.28 %) respectively, and Lymphocyte percent (49.00 and 46.03%). Also significant increases (P˂0.05) were observed in the WBC count between the control and the second group (7.0...
Journal of Al-Nahrain University-Science, 2016
Motor neuron cell is responsible for transfer neural instruct from brain to peripheral muscle thr... more Motor neuron cell is responsible for transfer neural instruct from brain to peripheral muscle through spinal cord; therefore any defect in these cells or spinal cord will affect motion. This study was designed to induce differentiation of Bone marrow mesenchymal stem cells (BM-MSCs) into neuron cells. BM-MSCs were isolated from bone stroma of femur and tibia of albino male mice and tested immunocytochemically for CD44, CD90, and CD105 expression and showed positive staining, while showed negative staining for CD34. Differentiation of BM-MSCs to motor neuron involved two main steps. Firs; induction of BM-MSCs by addition of 1mM mercaptoethanol (BME) in fetal bovine serum (FBS) in minimum essential medium MEM for 24 h and 2mM BME in free serum media for 2h. In the second step of induction retinoic acid, sonic hedgehog and nerve growth factor were added in free serum MEM for 4 days. Results revealed that the differentiation medium used was very efficient in directing the BM-MSCs to motor neural cell and showed positive reactivity to specific motor neural markers that used for detection of motor neuron cells like microtubule associated protein-2 antibodies and acetylcholine transferase antibody.
In Vitro Cellular & Developmental Biology - Animal, 1991
Iraqi Journal of Cancer and Medical Genetics, 2012
Asociación Española de Estudios Anglo-Norteamericanos. AEDEAN, 2020
The Iraqi Journal of Veterinary Medicine, 2010
The in vivo study was designed to evaluate the anticancer effects of these three types of the ext... more The in vivo study was designed to evaluate the anticancer effects of these three types of the extracts on the AM-3 (Murine mammary adenocarcinoma). The LD50 was (5 g/kg, 6 g/kg and 8 g/kg) for ethanolic, hot aqueous and cold aqueous extracts respectively. The therapeutic doses of extracts were daily injected subcutaneously for two weeks, three weeks and four weeks. The histopathological study revealed pathological changes in some organs; the group that treated with ethanolic extract showed sever deposition of amyloid in some organs like spleen. The blood vessel filled with inflammatory cells as neutrophiles and cells infiltration in the lung tissue. Hot and cold aqueous extracts showed inflammatory mononuclear cells infiltration in the kidney and lung, section with necrosis in most of the cancer cells in the lung tissue; with few necrotic changes in the normal tissue were noticed after one month of treatment by hot and cold aqueous extracts.
arXiv: Biological Physics, Dec 1, 2015
Conventional kinesin walks by a handover hand mechanism on the microtubule (MT) by taking ∼ 8nm d... more Conventional kinesin walks by a handover hand mechanism on the microtubule (MT) by taking ∼ 8nm discrete steps, and consumes one ATP molecule per step. The time needed to complete a single step is on the order of twenty microseconds. We show, using simulations of a coarse-grained model of the complex containing the two motor heads, the MT, and the coiled coil that in order to obtain quantitative agreement with experiments for the stepping kinetics hydrodynamic interactions (HI) have to be included. In simulations without hydrodynamic interactions spanning nearly twenty µs not a single step was completed in hundred trajectories. In sharp contrast, nearly 14% of the steps reached the target binding site within 6µs when HI were included. Somewhat surprisingly, there are qualitative differences in the diffusion pathways in simulations with and without HI. The extent of movement of the trailing head of kinesin on the MT during the diffusion stage of stepping is considerably greater in simulations with HI than in those without HI. Our results suggest that inclusion of HI is crucial in the accurate description of motility of other motors as well.
Iraqi Journal of Cancer and Medical Genetics, 2014
Iraqi Journal of Cancer and Medical Genetics, 2015
R egional immune response with mammary gland carcinoma was studied statistically. However the pro... more R egional immune response with mammary gland carcinoma was studied statistically. However the prognostic value remains conflicting. This study was conducted on thirty normal mice which were inoculated with mammary gland carcinoma. The tumour size of the animals under study were measured before and after laser irradiation by using a vernier and compared with that of non irradiated animals with laser (control group). The aim of this study was to evaluate the effect of low level laser (LLL) on increasing the response of immune system by stimulating the spleen action to inhibit the cancer cell activity and then decrease the tumour size of inoculated mice. The results of the gross observation showed enlargement in the irradiated spleen in comparison with that of non irradiated ones. It could be said that the enlargement which occurred in the spleen proved that there was an increase in immune response by using laser stimulation. From this study, it may concluded that low level laser(LLL) ...
The Iraqi Journal of Veterinary Medicine, Jun 30, 2010
In order to investigate the phytochemicals presence in the seeds of local plant of Datura stramon... more In order to investigate the phytochemicals presence in the seeds of local plant of Datura stramonium rather than alkaloids. For this purpose two types of Datura stramonium extract were prepared. The first was precipitated by acetone and the other was destructed by ether then precipitated with ammonium sulphate. The first extract yielded 2.5gram/ 5 gram of seeds weight; while, the second extract yielded 1.23 gram/ 5 gram of seed weight. Biochemical tests exhibited that lectin and trace amounts of glycosides were presented in the acetone precipitated extract. Whereas, the ether extract contained lectin, tannin, flavonoids, and trace amounts of alkaloids.