The Clinical Proteomic Technologies for Cancer (original) (raw)

HRas Proto-Oncogene, GTPase

Background

Catalog Number:

CPTC-HRAS-1

Target Antigen:

HRas Proto-Oncogene, GTPase

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

01/06/2026

Antigen Recognition(s):

Recombinant Full-length, Endogenous

Characterization Data [Compare Characterization Data]

IP

Click to enlarge image Immuno-precipitation screening of antibody CPTC-HRAS-1 in cell lysates of Mouse Embryonic Fibroblasts (MEFs) stably transfected with KRas4a, KRas4b, HRAS and NRAS and in MCF7 cell lysate. IP eluates were tested in WB using CPTC-HRAS-1 as detection antibody (IP-WB), The antibody showed specificity for HRAS. Expected MW is ~21KDa. The target was also pulled down in the MCF7 lysate. Click image to enlarge

Result: Negative

Immuno-precipitation screening of antibody CPTC-HRAS-1 in cell lysates of Mouse Embryonic Fibroblasts (MEFs) stably transfected with KRas4a, KRas4b, HRAS and NRAS and in MCF7 cell lysate. IP eluates were tested in WB using CPTC-HRAS-1 as detection antibody (IP-WB), The antibody showed specificity for HRAS. Expected MW is ~21KDa. The target was also pulled down in the MCF7 lysate.

NCI 60 Protein Array

Click to enlarge image Protein Array in which CPTC-HRAS-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

Result: Positive

Protein Array in which CPTC-HRAS-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).

Background

Catalog Number:

CPTC-HRAS-2

Target Antigen:

HRas Proto-Oncogene, GTPase

Isotype:

IgG2a

Species:

Mouse Monoclonal Antibody

Last Updated:

01/06/2026

Antigen Recognition(s):

Recombinant Full-length, Endogenous

Characterization Data [Compare Characterization Data]

CyTOF

Click to enlarge image Imaging mass cytometry on colon cancer tissue core using CPTC-HRAS-2 metal-labeled antibody. Data shows overlay of target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (testis, colon, and kidney) and cancer tissues (colon and ovarian). Click image to enlarge

Result: Positive

Imaging mass cytometry on colon cancer tissue core using CPTC-HRAS-2 metal-labeled antibody. Data shows overlay of target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (testis, colon, and kidney) and cancer tissues (colon and ovarian).

IP

Click to enlarge image Immuno-precipitation screening of antibody CPTC-HRAS-2 in cell lysates of Mouse Embryonic Fibroblasts (MEFs) stably transfected with KRas4a, KRas4b, HRAS and NRAS and in MCF7 cell lysate. IP eluates were tested in WB using CPTC-HRAS-1 as detection antibody (IP-WB), The antibody showed specificity for HRAS. Expected MW is ~21KDa. The target was also pulled down in the MCF7 lysate. Click image to enlarge

Result: Positive

Immuno-precipitation screening of antibody CPTC-HRAS-2 in cell lysates of Mouse Embryonic Fibroblasts (MEFs) stably transfected with KRas4a, KRas4b, HRAS and NRAS and in MCF7 cell lysate. IP eluates were tested in WB using CPTC-HRAS-1 as detection antibody (IP-WB), The antibody showed specificity for HRAS. Expected MW is ~21KDa. The target was also pulled down in the MCF7 lysate.

NCI 60 Protein Array

Click to enlarge image Protein Array in which CPTC-HRAS-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

Result: Positive

Protein Array in which CPTC-HRAS-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).

Background

Catalog Number:

CPTC-HRAS-3

Target Antigen:

HRas Proto-Oncogene, GTPase

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

01/06/2026

Antigen Recognition(s):

Recombinant Full-length, Endogenous

Characterization Data [Compare Characterization Data]

IP

Click to enlarge image Immuno-precipitation screening of antibody CPTC-HRAS-3 in cell lysates of Mouse Embryonic Fibroblasts (MEFs) stably transfected with KRas4a, KRas4b, HRAS and NRAS and in MCF7 cell lysate. IP eluates were tested in WB using CPTC-HRAS-1 as detection antibody (IP-WB), The antibody showed specificity for HRAS. Expected MW is ~21KDa. The target was also pulled down in the MCF7 lysate. Click image to enlarge

Result: Negative

Immuno-precipitation screening of antibody CPTC-HRAS-3 in cell lysates of Mouse Embryonic Fibroblasts (MEFs) stably transfected with KRas4a, KRas4b, HRAS and NRAS and in MCF7 cell lysate. IP eluates were tested in WB using CPTC-HRAS-1 as detection antibody (IP-WB), The antibody showed specificity for HRAS. Expected MW is ~21KDa. The target was also pulled down in the MCF7 lysate.

NCI 60 Protein Array

Click to enlarge image Protein Array in which CPTC-HRAS-3 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

Result: Positive

Protein Array in which CPTC-HRAS-3 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).

Background

Catalog Number:

CPTC-HRAS-4

Target Antigen:

HRas Proto-Oncogene, GTPase

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

01/06/2026

Antigen Recognition(s):

Recombinant Full-length, Endogenous

Characterization Data [Compare Characterization Data]

CyTOF

Click to enlarge image Imaging mass cytometry on ovarian cancer tissue core using CPTC-HRAS-4 metal-labeled antibody. Data shows overlay of target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (testis, colon, endometrium, appendix, bone marrow, breast, kidney, and lung) and cancer tissues (breast, colon, ovarian, lung, and prostate). Click image to enlarge

Result: Positive

Imaging mass cytometry on ovarian cancer tissue core using CPTC-HRAS-4 metal-labeled antibody. Data shows overlay of target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (testis, colon, endometrium, appendix, bone marrow, breast, kidney, and lung) and cancer tissues (breast, colon, ovarian, lung, and prostate).

Background

NCI Identification Number:

00490

Antigen Name:

HRas Proto-Oncogene, GTPase

CPTC Name:

CPTC-HRAS

Aliases:

HRas Proto-Oncogene, GTPase; V-Ha-Ras Harvey Rat Sarcoma Viral Oncogene Homolog; Harvey Rat Sarcoma Viral Oncogene Homolog; Transforming Protein P21; GTPase HRas; P21ras; HRAS1; Ras Family Small GTP Binding Protein H-Ras; Harvey Rat Sarcoma Viral Oncoprotein; Transformation Gene: Oncogene HAMSV; GTP- And GDP-Binding Peptide B; Ha-Ras1 Proto-Oncoprotein; C-Has/Bas P21 Protein; P19 H-RasIDX Protein; EC 3.6.5.2; C-BAS/HAS; C-HA-RAS1; H-RASIDX; C-H-RAS; H-Ras-1; C-H-Ras; Ha-Ras; HAMSV; RASH1; CTLO; HRAS

Function:

This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, cognitive disability, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene.HRAS (HRas Proto-Oncogene, GTPase) is a Protein Coding gene. Diseases associated with HRAS include Costello Syndrome and Nevus, Epidermal. Among its related pathways are Sertoli-Sertoli Cell Junction Dynamics and ERK Signaling. Gene Ontology (GO) annotations related to this gene include GTP binding and protein C-terminus binding. An important paralog of this gene is KRAS.Involved in the activation of Ras protein signal transduction (PubMed:22821884). Ras proteins bind GDP/GTP and possess intrinsic GTPase activity

Chromosomal Localization:

11p15.5

Expression System:

Baculovirus

Accession Number:

NP_005334.1

UniProt Accession Number:

P01112

DNA Source:

N/A

Immunogen:

Recombinant Full Length Protein

Vector Name:

xx

Extinction Coefficient:

Buffers:

Expressed Sequence:

MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGET
CLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQI
KRVKDSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQ
GVEDAFYTLVREIRQHKLRKLNPPDESGPGCMSCKCVLS

Native Sequence:

Calculated Isoelectric Point:

5.16

Molecular Weight:

21355

Last Updated:

11/17/2021

Characterization Data

SOPs

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