The Clinical Proteomic Technologies for Cancer (original) (raw)

Myeloperoxidase Peptide 1

Background

Catalog Number:

CPTC-MPO-2

RRID:

AB_2814785

Target Antigen:

Myeloperoxidase Peptide 1

Isotype:

IgG

Species:

Mouse Monoclonal Antibody

Last Updated:

07/19/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

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Characterization Data [Compare Characterization Data]

NCI 60 Protein Array

Click to enlarge image Protein Array in which CPTC-MPO-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

Result: Positive

Protein Array in which CPTC-MPO-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).

Western Blot - Tissue or Cell Lysate

Click to enlarge image Western blot using CPTC-MPO-2 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 83.9 kDa, 73.9 kDa, and 87.2 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

Result: Negative

Western blot using CPTC-MPO-2 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 83.9 kDa, 73.9 kDa, and 87.2 kDa. Molecular weight standards are also included (lane 1).

Western Blot - Tissue or Cell Lysate

Click to enlarge image Western blot using CPTC-MPO-2 as primary antibody against human lung (2), spleen (3), endometrium (4), breast (5), and ovary (6) tissue lysates. The expected molecular weight is 83.9 kDa, 73.9 kDa, and 87.2 kDa. Lung, spleen, and endometrium are presumed positive (MPO heavy chain). Breast and ovary are negative. Vinculin was used as a loading control. The immunoblot was conducted under reducing conditions. Click image to enlarge

Result: Presumed Positive (with additional bands)

Western blot using CPTC-MPO-2 as primary antibody against human lung (2), spleen (3), endometrium (4), breast (5), and ovary (6) tissue lysates. The expected molecular weight is 83.9 kDa, 73.9 kDa, and 87.2 kDa. Lung, spleen, and endometrium are presumed positive (MPO heavy chain). Breast and ovary are negative. Vinculin was used as a loading control. The immunoblot was conducted under reducing conditions.

Background

Catalog Number:

CPTC-MPO-3

Target Antigen:

Myeloperoxidase Peptide 1

Isotype:

IgG

Species:

Mouse Monoclonal Antibody

Last Updated:

07/19/2024

Antigen Recognition(s):

Peptide

Characterization Data [Compare Characterization Data]

NCI 60 Protein Array

Click to enlarge image Protein Array in which CPTC-MPO-3 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

Result: Positive

Protein Array in which CPTC-MPO-3 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).

Background

NCI Identification Number:

00478

Antigen Name:

Myeloperoxidase Peptide 1

CPTC Name:

CPTC-MPO Peptide 1

Aliases:

Myeloperoxidase; EC 1.11.1.7; EC 1.11.2.2; EC 1.11.1; MPO

Function:

Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils.MPO (Myeloperoxidase) is a Protein Coding gene. Diseases associated with MPO include Myeloperoxidase Deficiency and Alzheimer Disease. Among its related pathways are Transcriptional misregulation in cancer and Folate Metabolism. Gene Ontology (GO) annotations related to this gene include chromatin binding and heme binding. An important paralog of this gene is EPX.Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.

Chromosomal Localization:

17q22

Accession Number:

NP_000241.1

UniProt Accession Number:

P05164

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

IGLDLPALNMQR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1320

Last Updated:

12/17/2020

Characterization Data

SOPs

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