Ehab M B Mahdy | Agricultural Research Center (Egypt) (original) (raw)
Uploads
Thesis Chapters by Ehab M B Mahdy
The present study aimed to estimate genetic variability and determine the relationships among eig... more The present study aimed to estimate genetic variability and determine the relationships among eight Jew’s Mallow (Corchorus olitorius L.) accessions and five Lettuce (Lactuca sativa L.) accessions obtaining from National Gene Bank (NGB). This study was carried out 15 morphological traits of Jew’s Mallow and 11 characters for Lettuce, as well as karyotype and ISSR-PCR analyses. Significant differences were recorded for almost criteria of Jew’s Mallow, especially leaf length and leaf width, no. of leaves per plant, weight of harvested leaves and harvested index, except weight of total plant. Accessions 8 and 7 possessed higher values than others for important characteristics, leaf length and leaf width, no. of leaves per plant, weight of harvested leaves and harvested index. Estimate of heritability in broad sense was high (range from 67.56 – 93.55 %). Karyotype analysis confirmed that accession 8 was the highest values, while the accession 7 possessed the lowest values for chromosomal characters. Amplified DNA fragments produced a total 39 bands, ranging from 207 – 1807 bp. A total 36 bands were polymorphic with mean of polymorphism 92.31%. Investigated eight accessions was divided into two groups at distance 0.760. The first group includes accession 1; and the second group further divided into two subgroups. The highest similarity (0.99) was recorded between accessions 4 and 6. High significant differences were recorded for most traits specially position young leaf, position of leaves, size and weight of head. Accession 1 possessed the highest values for important leafy traits. The important leafy traits varied in heritability estimate (25.34 – 95.65%). The accession 5 recorded the highest values for chromosomal traits. Amplified DNA fragments produced a total of 25 bands ranging from 194 – 1247 bp. A total of 22 bands were polymorphic with average percentage of polymorphism was 83.30%. Investigated five accessions were divided into two groups at distance 0.95. The first group included accession 1 only. The second group was further divided into two subgroups at distance 0.99. The highest similarity (1.00) was recorded between accession 3 and accession 5.
Papers by Ehab M B Mahdy
Agronomía Colombiana
Inter-varietal hybridization is a powerful tool for genetic improvement and production of new gen... more Inter-varietal hybridization is a powerful tool for genetic improvement and production of new genotypes for a trait of interest. Four parents of faba beans (Vicia faba L.) were hybridized using agromorphological and molecular characterization to obtain genotypes resistant to the chocolate spot disease. The study was done at the Nubaria Research Station, Giza, Egypt. Eight traits including resistance to chocolate spot, days to flowering, plant height (cm), number of branches/plant, number of pods/plant, number of seeds/plant, 100-seed weight (g), and seed yield/plant were estimated during the three growth seasons of 2016/2017, 2017/2018, and 2018/2019. Genetic parameters revealed by RAPD and ISSR markers assessed the genetic variation of genotypes with their generations. Crosses 1 (P1 “Nubaria-1” x P2 “Sakha-1”), 2 (P1 “Nubaria-1” x P3 “T.W”), and 3 (P1 “Nubaria-1” x P4 “Camolina”) revealed high resistance to disease with high yield. Markers patterned specific loci of resistant paren...
Nutraceuticals Production from Plant Cell Factory
Al-Azhar Bulletin of Science
Plants
Determining the appropriate parents for breeding programs is the most important decision that pla... more Determining the appropriate parents for breeding programs is the most important decision that plant breeders must make to maximize the genetic variability and produce excellent recombinant genotypes. Several methods are used to identify genotypes with desirable phenotypic features for breeding experiments. In this study, five kalanchoe genotypes were morphologically characterized by assessing plant height, number of inflorescences, number of flowers, flower length, flower diameter and number of petals. The analysis showed the distinction of yellow kalanchoe in the plant height trait, while the orange kalanchoe was distinguished in the number of inflorescences, the number of flowers and flower length traits, whereas the violet kalanchoe possessed the largest flower diameter and the highest number of petals. The molecular profiling was performed by random amplified polymorphism DNA (RAPD), inter-simple sequence repeats (ISSR) and start codon targeted (SCoT)-polymerase chain reaction (...
The Date Palm Genome, Vol. 1, 2021
Current knowledge suggests that not all genomic information transmits across subsequent generatio... more Current knowledge suggests that not all genomic information transmits across subsequent generations. Variability in date palm genomes comes from the infidelity of DNA replication and segregation of unequal chromosomes. DNA lesions in nature are also the source of genomic alteration. Additionally, exposure of explants to external factors such as UV light and chemical mutagens can result in a new useful mutation. Biological cell systems deal naturally with the numerous DNA damages to maintain the date palm genome integrity by evolving several response systems such as checkpoint responses to various DNA damage types. Checkpoints are a well-known control mechanism in the plant cell cycle and respond to DNA replication breaks of dsDNA and diverse other types of DNA damage. Varied evidence indicates that genomic instability is probably the key reason for mutagenesis and the main factor in releasing new desirable mutants against abiotic stress. Thus, understanding how date palm tissues are...
International Journal of Pharma Sciences and Scientific Research, 2017
Jordan Journal of Biological Sciences, 2021
This work described the ten cowpea accessions using morphological, agronomical, and molecular cha... more This work described the ten cowpea accessions using morphological, agronomical, and molecular characterization and select the desirable cowpea accession for improvement. Twenty-four characteristics were quantitative attributes. Data demonstrated by the Principal Component Analysis (PCA) increasing in some components associated with a decrease in eigenvalues, which gave its max at three factors. The PCA was arranged into three main components, which altogether valued at 63.40%. The matrix of PCA data is standardized. The coordinates were calculated for Bi-plot mapping using the Perceptual Mapping (PERMAP). Radiata accessions (1 and 2) fell into a group. Unguiculata accessions are classified further into two sub-groups. The accessions (4 and 6) dropped into a sub-group, and the rest gathered into another sub-group. The PCR-based marker technique, SSR markers, were also analyzed. These markers gave a total of 64 alleles with 51.32% of polymorphism. A total of 26 alleles were polymorphi...
The Date Palm Genome, Vol. 1, 2021
The level of variation in date palm cultivars and among Phoenix species, and their structure of D... more The level of variation in date palm cultivars and among Phoenix species, and their structure of DNA molecules can play a beneficial role in the efficient utilization of germplasm resources. The background of evolutionary gene flow and diversity of cultivars are essential factors for these variabilities. Investigating the diversity has been utilized successfully in date palm genetic identification and improvement, a rapid increase in date palm genome sequences and DNA barcoding in date palm varieties. Furthermore, it is significant to verify the material obtained and analyze genetic diversity. Identification of genotypes is even related highly to individual palms. Date palm improvement can be achieved by recent and novel platforms such as clustered regularly interspaced short palindromic repeats (CRISPR), competitive allele specific PCR (KASP) markers and targeting induced local lesions in genomes (TILLING). We highlight the most useful various molecular markers and DNA barcoding tha...
International Journal of Pharma Sciences and Scientific Research, 2017
This study was to screen the morphological, phytochemical attributes and describe the similarity ... more This study was to screen the morphological, phytochemical attributes and describe the similarity and diversity in terms of RAPD and ISSR profiles of four Jatropha species (J. multifida, J. gossypifolia, J. integrrima and J. curcas). As well, investigate genetic diversity among them for using in conserving at Gene-Banks and genetic improvement. It appeared that show the variation in twenty-three quantitative and qualitative attributes among Jatropha species under Egyptian condition. The results of Pearson correlation among twelve quantitative revealed high significant positive correlations. Negative correlation observed between petiole length and other traits except seed breadth, whereas it was-0.97 when be compared with pod length. In PCA studied, it noted that leaf length, leaf width and petiole length were contributing most to the separation among species. The result showed that all secondary metabolites analyzed were present in all leaves of Jatropha species studied but at different concentrations and it obtained confirm the relatedness of these species and spotlight these important phytochemicals in the species. At molecular study, four RAPD-primers displayed a total of 66 amplified fragments, in which 50 (75.76%) were polymorphic fragments. The number of total amplified fragments scored per primer ranged from 10 (primer OP-D09) to 24 (primer OP-Z03). Thirty-six out of 66 RAPD-PCR fragments were found to be useful as cultivar specific markers. In ISSR analysis, 5 of the ISSR primers generated variable banding patterns. A total of 63 out of 90 ISSR fragments were polymorphic. 36 amplified fragments were considered as cultivar-specific markers. Results of the combination of the banding patterns of both techniques, data exhibited that the most two closely related species were multifida and gossypifolia with the highest similarity index (1.00). On the other hand, the two most distantly related species were curcas and integerrima with no similarity index (0.00). The 9 primers of RAPD and ISSR yielded 113 polymorphic markers that unambiguously discriminated 4 genotypes into three clusters. In conclusion, polymorphisms of both could be used as efficient tools for the detection of similarities and phylogenetic relationships of the studied genotypes, which could be useful in the breeding programs.
Genetic variability and similarity between eight accessions of Jew’s Mallow (Corchorus olitorius ... more Genetic variability and similarity between eight accessions of Jew’s Mallow (Corchorus olitorius L.) were studied. These accessions obtained from National Gene Bank (NGB), Agricultural Research Center (ARC), Egypt. The mean of 10 healthy well developed plant materials for each were characterized for 15 morphological characteristics, as well as karyotype characteristics were conducted. Significant differences among the eight accessions were recorded, except weight of total plant. Accessions 8 and 7 possessed higher values than other accessions for important characteristics, leaf length and leaf width, no. of leaves per plant, weight of harvested leaves and harvested index. Heritability estimate in broad sense was high (range from 67.56 – 93.55 %) for important criteria. Karyotype analysis confirmed that accessions 8 and 7 possessed the highest and lowest values respectively for chromosome total area, total length, long and short arm. The cytogenetical characteristics for all studied accessions were recorded as well as the results confirmed that chromosome 2 was longer than other chromosomes. Hierarchical clustering analysis was conducted to generate a dendrogram illustrating possible relationships among accessions based on morphological and cytological attributes. Investigated eight accessions are divided into two groups at distance 0.734. The first group includes accession 1; and the second group further divided into two subgroups. The highest similarity (1.000) was recorded between accessions 3 and 5, while the lowest similarity (0.734) was between accession 1 and 7. The results confirmed that the similarity between different accessions was depended on morphological criteria more than other as cytogenetical criteria.
Teaching Documents by Ehab M B Mahdy
The present study aimed to estimate genetic variability and determine the relationships among eig... more The present study aimed to estimate genetic variability and determine the relationships among eight Jew’s Mallow (Corchorus olitorius L.) accessions and five Lettuce (Lactuca sativa L.) accessions obtaining from National Gene Bank (NGB). This study was carried out 15 morphological traits of Jew’s Mallow and 11 characters for Lettuce, as well as karyotype and ISSR-PCR analyses. Significant differences were recorded for almost criteria of Jew’s Mallow, especially leaf length and leaf width, no. of leaves per plant, weight of harvested leaves and harvested index, except weight of total plant. Accessions 8 and 7 possessed higher values than others for important characteristics, leaf length and leaf width, no. of leaves per plant, weight of harvested leaves and harvested index. Estimate of heritability in broad sense was high (range from 67.56 – 93.55 %). Karyotype analysis confirmed that accession 8 was the highest values, while the accession 7 possessed the lowest values for chromosomal characters. Amplified DNA fragments produced a total 39 bands, ranging from 207 – 1807 bp. A total 36 bands were polymorphic with mean of polymorphism 92.31%. Investigated eight accessions was divided into two groups at distance 0.760. The first group includes accession 1; and the second group further divided into two subgroups. The highest similarity (0.99) was recorded between accessions 4 and 6. High significant differences were recorded for most traits specially position young leaf, position of leaves, size and weight of head. Accession 1 possessed the highest values for important leafy traits. The important leafy traits varied in heritability estimate (25.34 – 95.65%). The accession 5 recorded the highest values for chromosomal traits. Amplified DNA fragments produced a total of 25 bands ranging from 194 – 1247 bp. A total of 22 bands were polymorphic with average percentage of polymorphism was 83.30%. Investigated five accessions were divided into two groups at distance 0.95. The first group included accession 1 only. The second group was further divided into two subgroups at distance 0.99. The highest similarity (1.00) was recorded between accession 3 and accession 5.
Agronomía Colombiana
Inter-varietal hybridization is a powerful tool for genetic improvement and production of new gen... more Inter-varietal hybridization is a powerful tool for genetic improvement and production of new genotypes for a trait of interest. Four parents of faba beans (Vicia faba L.) were hybridized using agromorphological and molecular characterization to obtain genotypes resistant to the chocolate spot disease. The study was done at the Nubaria Research Station, Giza, Egypt. Eight traits including resistance to chocolate spot, days to flowering, plant height (cm), number of branches/plant, number of pods/plant, number of seeds/plant, 100-seed weight (g), and seed yield/plant were estimated during the three growth seasons of 2016/2017, 2017/2018, and 2018/2019. Genetic parameters revealed by RAPD and ISSR markers assessed the genetic variation of genotypes with their generations. Crosses 1 (P1 “Nubaria-1” x P2 “Sakha-1”), 2 (P1 “Nubaria-1” x P3 “T.W”), and 3 (P1 “Nubaria-1” x P4 “Camolina”) revealed high resistance to disease with high yield. Markers patterned specific loci of resistant paren...
Nutraceuticals Production from Plant Cell Factory
Al-Azhar Bulletin of Science
Plants
Determining the appropriate parents for breeding programs is the most important decision that pla... more Determining the appropriate parents for breeding programs is the most important decision that plant breeders must make to maximize the genetic variability and produce excellent recombinant genotypes. Several methods are used to identify genotypes with desirable phenotypic features for breeding experiments. In this study, five kalanchoe genotypes were morphologically characterized by assessing plant height, number of inflorescences, number of flowers, flower length, flower diameter and number of petals. The analysis showed the distinction of yellow kalanchoe in the plant height trait, while the orange kalanchoe was distinguished in the number of inflorescences, the number of flowers and flower length traits, whereas the violet kalanchoe possessed the largest flower diameter and the highest number of petals. The molecular profiling was performed by random amplified polymorphism DNA (RAPD), inter-simple sequence repeats (ISSR) and start codon targeted (SCoT)-polymerase chain reaction (...
The Date Palm Genome, Vol. 1, 2021
Current knowledge suggests that not all genomic information transmits across subsequent generatio... more Current knowledge suggests that not all genomic information transmits across subsequent generations. Variability in date palm genomes comes from the infidelity of DNA replication and segregation of unequal chromosomes. DNA lesions in nature are also the source of genomic alteration. Additionally, exposure of explants to external factors such as UV light and chemical mutagens can result in a new useful mutation. Biological cell systems deal naturally with the numerous DNA damages to maintain the date palm genome integrity by evolving several response systems such as checkpoint responses to various DNA damage types. Checkpoints are a well-known control mechanism in the plant cell cycle and respond to DNA replication breaks of dsDNA and diverse other types of DNA damage. Varied evidence indicates that genomic instability is probably the key reason for mutagenesis and the main factor in releasing new desirable mutants against abiotic stress. Thus, understanding how date palm tissues are...
International Journal of Pharma Sciences and Scientific Research, 2017
Jordan Journal of Biological Sciences, 2021
This work described the ten cowpea accessions using morphological, agronomical, and molecular cha... more This work described the ten cowpea accessions using morphological, agronomical, and molecular characterization and select the desirable cowpea accession for improvement. Twenty-four characteristics were quantitative attributes. Data demonstrated by the Principal Component Analysis (PCA) increasing in some components associated with a decrease in eigenvalues, which gave its max at three factors. The PCA was arranged into three main components, which altogether valued at 63.40%. The matrix of PCA data is standardized. The coordinates were calculated for Bi-plot mapping using the Perceptual Mapping (PERMAP). Radiata accessions (1 and 2) fell into a group. Unguiculata accessions are classified further into two sub-groups. The accessions (4 and 6) dropped into a sub-group, and the rest gathered into another sub-group. The PCR-based marker technique, SSR markers, were also analyzed. These markers gave a total of 64 alleles with 51.32% of polymorphism. A total of 26 alleles were polymorphi...
The Date Palm Genome, Vol. 1, 2021
The level of variation in date palm cultivars and among Phoenix species, and their structure of D... more The level of variation in date palm cultivars and among Phoenix species, and their structure of DNA molecules can play a beneficial role in the efficient utilization of germplasm resources. The background of evolutionary gene flow and diversity of cultivars are essential factors for these variabilities. Investigating the diversity has been utilized successfully in date palm genetic identification and improvement, a rapid increase in date palm genome sequences and DNA barcoding in date palm varieties. Furthermore, it is significant to verify the material obtained and analyze genetic diversity. Identification of genotypes is even related highly to individual palms. Date palm improvement can be achieved by recent and novel platforms such as clustered regularly interspaced short palindromic repeats (CRISPR), competitive allele specific PCR (KASP) markers and targeting induced local lesions in genomes (TILLING). We highlight the most useful various molecular markers and DNA barcoding tha...
International Journal of Pharma Sciences and Scientific Research, 2017
This study was to screen the morphological, phytochemical attributes and describe the similarity ... more This study was to screen the morphological, phytochemical attributes and describe the similarity and diversity in terms of RAPD and ISSR profiles of four Jatropha species (J. multifida, J. gossypifolia, J. integrrima and J. curcas). As well, investigate genetic diversity among them for using in conserving at Gene-Banks and genetic improvement. It appeared that show the variation in twenty-three quantitative and qualitative attributes among Jatropha species under Egyptian condition. The results of Pearson correlation among twelve quantitative revealed high significant positive correlations. Negative correlation observed between petiole length and other traits except seed breadth, whereas it was-0.97 when be compared with pod length. In PCA studied, it noted that leaf length, leaf width and petiole length were contributing most to the separation among species. The result showed that all secondary metabolites analyzed were present in all leaves of Jatropha species studied but at different concentrations and it obtained confirm the relatedness of these species and spotlight these important phytochemicals in the species. At molecular study, four RAPD-primers displayed a total of 66 amplified fragments, in which 50 (75.76%) were polymorphic fragments. The number of total amplified fragments scored per primer ranged from 10 (primer OP-D09) to 24 (primer OP-Z03). Thirty-six out of 66 RAPD-PCR fragments were found to be useful as cultivar specific markers. In ISSR analysis, 5 of the ISSR primers generated variable banding patterns. A total of 63 out of 90 ISSR fragments were polymorphic. 36 amplified fragments were considered as cultivar-specific markers. Results of the combination of the banding patterns of both techniques, data exhibited that the most two closely related species were multifida and gossypifolia with the highest similarity index (1.00). On the other hand, the two most distantly related species were curcas and integerrima with no similarity index (0.00). The 9 primers of RAPD and ISSR yielded 113 polymorphic markers that unambiguously discriminated 4 genotypes into three clusters. In conclusion, polymorphisms of both could be used as efficient tools for the detection of similarities and phylogenetic relationships of the studied genotypes, which could be useful in the breeding programs.
Genetic variability and similarity between eight accessions of Jew’s Mallow (Corchorus olitorius ... more Genetic variability and similarity between eight accessions of Jew’s Mallow (Corchorus olitorius L.) were studied. These accessions obtained from National Gene Bank (NGB), Agricultural Research Center (ARC), Egypt. The mean of 10 healthy well developed plant materials for each were characterized for 15 morphological characteristics, as well as karyotype characteristics were conducted. Significant differences among the eight accessions were recorded, except weight of total plant. Accessions 8 and 7 possessed higher values than other accessions for important characteristics, leaf length and leaf width, no. of leaves per plant, weight of harvested leaves and harvested index. Heritability estimate in broad sense was high (range from 67.56 – 93.55 %) for important criteria. Karyotype analysis confirmed that accessions 8 and 7 possessed the highest and lowest values respectively for chromosome total area, total length, long and short arm. The cytogenetical characteristics for all studied accessions were recorded as well as the results confirmed that chromosome 2 was longer than other chromosomes. Hierarchical clustering analysis was conducted to generate a dendrogram illustrating possible relationships among accessions based on morphological and cytological attributes. Investigated eight accessions are divided into two groups at distance 0.734. The first group includes accession 1; and the second group further divided into two subgroups. The highest similarity (1.000) was recorded between accessions 3 and 5, while the lowest similarity (0.734) was between accession 1 and 7. The results confirmed that the similarity between different accessions was depended on morphological criteria more than other as cytogenetical criteria.