GTF2I, CPTAC-1349 - CPTAC Assay Portal (original) (raw)

Official Gene Symbol

GTF2I

Peptide Modified Sequence

GREFS[+80.0]FEAWNAK

Modification Type

Phospho (ST)

Protein - Site of Modification

722

Peptide - Site of Modification

5

Peptide Start

718

Peptide End

729

CPTAC ID

CPTAC-1349

Peptide Molecular Mass

1,520.6449

Species

Homo sapiens (Human)

Assay Type

Enrichment MRM

Enrichment Method

Fe(III)-NTA

Matrix

digested cell lysate

Submitting Laboratory

Broad Institute

Submitting Lab PI

Steven A. Carr

Publication

View Details (opens in a new window)

Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. Reduced-representation Phosphosignatures Measured by Quantitative Targeted MS Capture Cellular States and Enable Large-scale Comparison of Drug-induced Phenotypes. Abelin JG1, Patel J1, Lu X1, Feeney CM1, Fagbami L1, Creech AL1, Hu R1, Lam D1, Davison D1, Pino L1, Qiao JW1, Kuhn E1, Officer A1, Li J2, Abbatiello S1, Subramanian A1, Sidman R2, Snyder E3, Carr SA1, Jaffe JD4.

View Details (opens in a new window)

Mol Cell Proteomics. 2014 Jul;13(7):1690-704. doi: 10.1074/mcp.M113.036392. Epub 2014 Apr 9. Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mertins P1, Yang F2, Liu T2, Mani DR3, Petyuk VA2, Gillette MA3, Clauser KR3, Qiao JW3, Gritsenko MA2, Moore RJ2, Levine DA4, Townsend R5, Erdmann-Gilmore P5, Snider JE5, Davies SR5, Ruggles KV6, Fenyo D6, Kitchens RT5, Li S5, Olvera N4, Dao F4, Rodriguez H7, Chan DW8, Liebler D9, White F10, Rodland KD2, Mills GB11, Smith RD2, Paulovich AG12, Ellis M5, Carr SA1

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | b7-98 (2+) | 8 | 7.3 | 6 | 11 | 10.4 | 10.8 | 13.6 | 12.7 | 12.4 | 15 | 15 | 15 | | y4 (1+) | 4.6 | 7 | 5.2 | 6.8 | 13.2 | 13.3 | 8.2 | 14.9 | 14.3 | 15 | 15 | 15 | | y5 (1+) | 5.6 | 5.9 | 4.2 | 7.8 | 12.5 | 9.1 | 9.6 | 13.8 | 10 | 15 | 15 | 15 | | sum | 5.4 | 4.2 | 3.5 | 7.4 | 11 | 10.3 | 9.2 | 11.8 | 10.9 | 15 | 15 | 15 |