SHC1, CPTAC-1366 - CPTAC Assay Portal (original) (raw)
Official Gene Symbol
SHC1
Peptide Modified Sequence
ELFDDPSY[+80.0]VNVQNLDK
Modification Type
Phospho (Y)
Protein - Site of Modification
427
Peptide - Site of Modification
8
Peptide Start
420
Peptide End
435
CPTAC ID
CPTAC-1366
Peptide Molecular Mass
1,974.8612
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
Fe(III)-NTA
Matrix
digested cell lysate
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr
Publication
View Details (opens in a new window)
Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. Reduced-representation Phosphosignatures Measured by Quantitative Targeted MS Capture Cellular States and Enable Large-scale Comparison of Drug-induced Phenotypes. Abelin JG1, Patel J1, Lu X1, Feeney CM1, Fagbami L1, Creech AL1, Hu R1, Lam D1, Davison D1, Pino L1, Qiao JW1, Kuhn E1, Officer A1, Li J2, Abbatiello S1, Subramanian A1, Sidman R2, Snyder E3, Carr SA1, Jaffe JD4.
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Mol Cell Proteomics. 2014 Jul;13(7):1690-704. doi: 10.1074/mcp.M113.036392. Epub 2014 Apr 9. Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mertins P1, Yang F2, Liu T2, Mani DR3, Petyuk VA2, Gillette MA3, Clauser KR3, Qiao JW3, Gritsenko MA2, Moore RJ2, Levine DA4, Townsend R5, Erdmann-Gilmore P5, Snider JE5, Davies SR5, Ruggles KV6, Fenyo D6, Kitchens RT5, Li S5, Olvera N4, Dao F4, Rodriguez H7, Chan DW8, Liebler D9, White F10, Rodland KD2, Mills GB11, Smith RD2, Paulovich AG12, Ellis M5, Carr SA1
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV
| n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y11 (2+) | 7.9 | 5.4 | 2.5 | 9.2 | 8.8 | 8.9 | 12.1 | 10.3 | 9.2 | 15 | 15 | 15 |
| y5 (1+) | 4.6 | 5.1 | 3.7 | 5.7 | 9.4 | 9.9 | 7.3 | 10.7 | 10.6 | 14 | 15 | 15 |
| y7 (1+) | 3.6 | 7 | 4.4 | 3.3 | 10.5 | 10.2 | 4.9 | 12.6 | 11.1 | 14 | 15 | 15 |
| sum | 6.6 | 5 | 2.2 | 8.8 | 9 | 9.1 | 11 | 10.3 | 9.4 | 15 | 15 | 15 |