MAPK1, CPTAC-870 - CPTAC Assay Portal (original) (raw)

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Protein Sequence hover to view complete sequence

non-CPTAC-6107: View additional ELIFEETAR data
CPTAC-871: View additional ELIFEETAR data

Data source: UniProt

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

All other points link out to UniProt

Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1352 Collapse assay details

Data source: Panorama

Official Gene Symbol

MAPK1

Peptide Modified Sequence

VADPDHDHTGFLT[+80.0]EY[+80.0]VATR

Modification Type

Phospho (ST), Phospho (Y)

Protein - Site of Modification

185, 187

Peptide - Site of Modification

13, 15

Peptide Start

173

Peptide End

191

CPTAC ID

CPTAC-1352

Peptide Molecular Mass

2,302.9297

Species

Homo sapiens (Human)

Assay Type

Enrichment PRM

Enrichment Method

N/A

Matrix

digested cell lysate

Submitting Laboratory

Broad Institute

Submitting Lab PI

Steven A. Carr

Publication

View Details (opens in a new window)

Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. Reduced-representation Phosphosignatures Measured by Quantitative Targeted MS Capture Cellular States and Enable Large-scale Comparison of Drug-induced Phenotypes. Abelin JG1, Patel J1, Lu X1, Feeney CM1, Fagbami L1, Creech AL1, Hu R1, Lam D1, Davison D1, Pino L1, Qiao JW1, Kuhn E1, Officer A1, Li J2, Abbatiello S1, Subramanian A1, Sidman R2, Snyder E3, Carr SA1, Jaffe JD4.

View Details (opens in a new window)

Mol Cell Proteomics. 2014 Jul;13(7):1690-704. doi: 10.1074/mcp.M113.036392. Epub 2014 Apr 9. Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mertins P1, Yang F2, Liu T2, Mani DR3, Petyuk VA2, Gillette MA3, Clauser KR3, Qiao JW3, Gritsenko MA2, Moore RJ2, Levine DA4, Townsend R5, Erdmann-Gilmore P5, Snider JE5, Davies SR5, Ruggles KV6, Fenyo D6, Kitchens RT5, Li S5, Olvera N4, Dao F4, Rodriguez H7, Chan DW8, Liebler D9, White F10, Rodland KD2, Mills GB11, Smith RD2, Paulovich AG12, Ellis M5, Carr SA1

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

Quantiva

Internal Standard

light stable isotope peptide

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C and 15N V

LC

Easy NanoLC1000

Column Packing

Reprosil C18, 1.9um, 200A

Column Dimensions

0.075 x 150 mm

Flow Rate

200 nL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y16-98 (2+) | 7.6 | 5.7 | 7.5 | 14.2 | 10.5 | 10.4 | 16.1 | 11.9 | 12.8 | 15 | 15 | 15 | | y5 (1+) | 5.2 | 5 | 6.4 | 7.6 | 10.6 | 11 | 9.2 | 11.7 | 12.7 | 15 | 15 | 15 | | y7-98 (1+) | 6.3 | 4.6 | 3 | 6.2 | 9.4 | 5.3 | 8.8 | 10.5 | 6.1 | 15 | 15 | 15 | | sum | 5 | 3.8 | 3.9 | 5.6 | 9.3 | 7.6 | 7.5 | 10 | 8.5 | 15 | 15 | 15 |

Additional Resources and Comments

Assay Details for CPTAC-1353 Collapse assay details

Data source: Panorama

Official Gene Symbol

MAPK1

Peptide Modified Sequence

VADPDHDHTGFLT[+80.0]EYVATR

Modification Type

Phospho (ST)

Protein - Site of Modification

185

Peptide - Site of Modification

13

Peptide Start

173

Peptide End

191

CPTAC ID

CPTAC-1353

Peptide Molecular Mass

2,222.9634

Species

Homo sapiens (Human)

Assay Type

Enrichment PRM

Enrichment Method

N/A

Matrix

digested cell lysate

Submitting Laboratory

Broad Institute

Submitting Lab PI

Steven A. Carr

Publication

View Details (opens in a new window)

Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. Reduced-representation Phosphosignatures Measured by Quantitative Targeted MS Capture Cellular States and Enable Large-scale Comparison of Drug-induced Phenotypes. Abelin JG1, Patel J1, Lu X1, Feeney CM1, Fagbami L1, Creech AL1, Hu R1, Lam D1, Davison D1, Pino L1, Qiao JW1, Kuhn E1, Officer A1, Li J2, Abbatiello S1, Subramanian A1, Sidman R2, Snyder E3, Carr SA1, Jaffe JD4.

View Details (opens in a new window)

Mol Cell Proteomics. 2014 Jul;13(7):1690-704. doi: 10.1074/mcp.M113.036392. Epub 2014 Apr 9. Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mertins P1, Yang F2, Liu T2, Mani DR3, Petyuk VA2, Gillette MA3, Clauser KR3, Qiao JW3, Gritsenko MA2, Moore RJ2, Levine DA4, Townsend R5, Erdmann-Gilmore P5, Snider JE5, Davies SR5, Ruggles KV6, Fenyo D6, Kitchens RT5, Li S5, Olvera N4, Dao F4, Rodriguez H7, Chan DW8, Liebler D9, White F10, Rodland KD2, Mills GB11, Smith RD2, Paulovich AG12, Ellis M5, Carr SA1

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

Quantiva

Internal Standard

light stable isotope peptide

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C and 15N V

LC

Easy NanoLC1000

Column Packing

Reprosil C18, 1.9um, 200A

Column Dimensions

0.075 x 150 mm

Flow Rate

200 nL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | b11 (1+) | 29.4 | 16.5 | 23.7 | 24.3 | 16.6 | 25.8 | 38.1 | 23.4 | 35 | 12 | 13 | 13 | | y6 (1+) | 12.4 | 14 | 6.5 | 11.4 | 12.1 | 12.7 | 16.8 | 18.5 | 14.3 | 15 | 15 | 15 | | y7-98 (1+) | 8.2 | 9.4 | 6.8 | 11.9 | 9.2 | 8.5 | 14.5 | 13.2 | 10.9 | 15 | 15 | 15 | | sum | 7.7 | 8.8 | 6.2 | 9.9 | 9.3 | 9.3 | 12.5 | 12.8 | 11.2 | 15 | 15 | 15 |

Additional Resources and Comments

Assay Details for CPTAC-1354 Collapse assay details

Data source: Panorama

Official Gene Symbol

MAPK1

Peptide Modified Sequence

VADPDHDHTGFLTEY[+80.0]VATR

Modification Type

Phospho (Y)

Protein - Site of Modification

187

Peptide - Site of Modification

15

Peptide Start

173

Peptide End

191

CPTAC ID

CPTAC-1354

Peptide Molecular Mass

2,222.9634

Species

Homo sapiens (Human)

Assay Type

Enrichment PRM

Enrichment Method

N/A

Matrix

digested cell lysate

Submitting Laboratory

Broad Institute

Submitting Lab PI

Steven A. Carr

Publication

View Details (opens in a new window)

Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. Reduced-representation Phosphosignatures Measured by Quantitative Targeted MS Capture Cellular States and Enable Large-scale Comparison of Drug-induced Phenotypes. Abelin JG1, Patel J1, Lu X1, Feeney CM1, Fagbami L1, Creech AL1, Hu R1, Lam D1, Davison D1, Pino L1, Qiao JW1, Kuhn E1, Officer A1, Li J2, Abbatiello S1, Subramanian A1, Sidman R2, Snyder E3, Carr SA1, Jaffe JD4.

View Details (opens in a new window)

Mol Cell Proteomics. 2014 Jul;13(7):1690-704. doi: 10.1074/mcp.M113.036392. Epub 2014 Apr 9. Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mertins P1, Yang F2, Liu T2, Mani DR3, Petyuk VA2, Gillette MA3, Clauser KR3, Qiao JW3, Gritsenko MA2, Moore RJ2, Levine DA4, Townsend R5, Erdmann-Gilmore P5, Snider JE5, Davies SR5, Ruggles KV6, Fenyo D6, Kitchens RT5, Li S5, Olvera N4, Dao F4, Rodriguez H7, Chan DW8, Liebler D9, White F10, Rodland KD2, Mills GB11, Smith RD2, Paulovich AG12, Ellis M5, Carr SA1

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

Quantiva

Internal Standard

light stable isotope peptide

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C and 15N V

LC

Easy NanoLC1000

Column Packing

Reprosil C18, 1.9um, 200A

Column Dimensions

0.075 x 150 mm

Flow Rate

200 nL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | b11 (2+) | 8.3 | 5.7 | 6 | 8.5 | 9.1 | 10.3 | 11.9 | 10.7 | 11.9 | 15 | 15 | 15 | | y5 (1+) | 7.5 | 3.8 | 5.1 | 7.6 | 9.3 | 9.7 | 10.7 | 10 | 11 | 15 | 15 | 15 | | y7 (1+) | 4.9 | 2.8 | 5.3 | 5.5 | 7.8 | 9.8 | 7.4 | 8.3 | 11.1 | 15 | 15 | 15 | | sum | 5.5 | 2.7 | 4.8 | 6 | 8 | 9.7 | 8.1 | 8.4 | 10.8 | 15 | 15 | 15 |

Additional Resources and Comments

Assay Details for non-CPTAC-6256 Collapse assay details

Data source: Panorama

Official Gene Symbol

MAPK1

Peptide Modified Sequence

VADPDHDHTGFLT[+79.966331]EY[+79.966331]VATR

Modification Type

Phospho (Y)

Protein - Site of Modification

N/A

Peptide - Site of Modification

8

Peptide Start

173

Peptide End

191

CPTAC ID

non-CPTAC-6256

Peptide Molecular Mass

2,302.9297

Species

Human

Assay Type

Enrichment PRM

Enrichment Method

N/A

Matrix

SKBR3 cells

Submitting Laboratory

Cell Signaling Technology

Submitting Lab PI

Yiying Zhu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

Orbitrap Q Exactive (Thermo)

Internal Standard

Heavy isotope-labeled peptides

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C and 15N V

LC

EASY-nLC II (Thermo)

Column Packing

self-packed

Column Dimensions

ID 100um, length 15cm

Flow Rate

300 nL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y7-98 (1+) | 8 | 5.2 | 3.2 | 9.9 | 4.9 | 2.9 | 12.7 | 7.1 | 4.3 | 15 | 15 | 15 | | y16-98 (2+) | 10 | 5.5 | 3.1 | 11.8 | 5.6 | 2.9 | 15.5 | 7.8 | 4.2 | 15 | 15 | 15 | | y15 (2+) | 11.7 | 4.8 | 3.2 | 10.9 | 5.5 | 3.2 | 16 | 7.3 | 4.5 | 15 | 15 | 15 | | y15 (3+) | 10.6 | 3.3 | 2.9 | 15.3 | 5.2 | 3.1 | 18.6 | 6.2 | 4.2 | 15 | 15 | 15 | | y16-98 (3+) | 8.8 | 4.6 | 2.3 | 15.5 | 5.5 | 2.1 | 17.8 | 7.2 | 3.1 | 15 | 15 | 15 | | sum | 6 | 4.6 | 2.5 | 8.3 | 4.9 | 2.3 | 10.2 | 6.7 | 3.4 | 15 | 15 | 15 |

Additional Resources and Comments

Assay Details for non-CPTAC-6255 Collapse assay details

Data source: Panorama

Official Gene Symbol

MAPK1

Peptide Modified Sequence

VADPDHDHTGFLT[+79.966331]EYVATR

Modification Type

Phospho (ST)

Protein - Site of Modification

N/A

Peptide - Site of Modification

8

Peptide Start

173

Peptide End

191

CPTAC ID

non-CPTAC-6255

Peptide Molecular Mass

2,222.9634

Species

Human

Assay Type

Enrichment PRM

Enrichment Method

N/A

Matrix

SKBR3 cells

Submitting Laboratory

Cell Signaling Technology

Submitting Lab PI

Yiying Zhu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

Orbitrap Q Exactive (Thermo)

Internal Standard

Heavy isotope-labeled peptides

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C and 15N V

LC

EASY-nLC II (Thermo)

Column Packing

self-packed

Column Dimensions

ID 100um, length 15cm

Flow Rate

300 nL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y8-98 (1+) | 7 | 5.8 | 4.9 | 7.2 | 12 | 5.8 | 10 | 13.3 | 7.6 | 15 | 15 | 15 | | y7-98 (1+) | 7.5 | 5.1 | 4.3 | 13.2 | 11.7 | 6 | 15.2 | 12.8 | 7.4 | 15 | 15 | 15 | | y5 (1+) | 5.9 | 7.5 | 4.3 | 10.2 | 12 | 6.2 | 11.8 | 14.2 | 7.5 | 15 | 15 | 15 | | y16-98 (2+) | 4.7 | 6.5 | 5 | 13 | 12.6 | 5.7 | 13.8 | 14.2 | 7.6 | 15 | 15 | 15 | | y15 (2+) | 5.1 | 5.7 | 4.1 | 8.9 | 12.5 | 6.5 | 10.3 | 13.7 | 7.7 | 15 | 15 | 15 | | y16 (2+) | 7.8 | 7.8 | 4.5 | 15.1 | 13.2 | 6.7 | 17 | 15.3 | 8.1 | 15 | 15 | 15 | | sum | 4.6 | 6 | 4.1 | 10.7 | 12.1 | 5.8 | 11.6 | 13.5 | 7.1 | 15 | 15 | 15 |

Additional Resources and Comments

Assay Details for CPTAC-5812 Collapse assay details

Data source: Panorama

Official Gene Symbol

MAPK1

Peptide Modified Sequence

VADPDHDHTGFLT[+79.966331]EY[+79.966331]VATR

Modification Type

Phospho (Y)

Protein - Site of Modification

N/A

Peptide - Site of Modification

3

Peptide Start

173

Peptide End

191

CPTAC ID

CPTAC-5812

Peptide Molecular Mass

2,302.9297

Species

Homo sapiens (Human)

Assay Type

Enrichment PRM

Enrichment Method

N/A

Matrix

Cell Lysate

Submitting Laboratory

Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute

Submitting Lab PI

Amanda Paulovich, John Koomen, Steven A. Carr

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

SCIEX 5500 / Thermo Quantiva

Internal Standard

Stable Isotope Labeled Standard

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C and 15N V

LC

Dionex Ultimate 3000

Column Packing

C18 PepMap 100

Column Dimensions

75 micron ID x 25 cm length

Flow Rate

300 nL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y15 (2+) | 12.1 | 15.4 | 14.5 | 19.8 | 21.5 | 18.5 | 23.2 | 26.4 | 23.5 | 16 | 15 | 16 | | y15 (3+) | 15.8 | 11.6 | 11.9 | 20.9 | 21.5 | 16.8 | 26.2 | 24.4 | 20.6 | 16 | 15 | 16 | | sum | 13.9 | 12.6 | 11.8 | 19.9 | 21.2 | 17.1 | 24.3 | 24.7 | 20.8 | 16 | 15 | 16 |

Additional Resources and Comments

Assay Details for CPTAC-5811 Collapse assay details

Data source: Panorama

Official Gene Symbol

MAPK1

Peptide Sequence

VADPDHDHTGFLTEYVATR

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

3

Peptide Start

173

Peptide End

191

CPTAC ID

CPTAC-5811

Peptide Molecular Mass

2,142.9970

Species

Homo sapiens (Human)

Assay Type

Enrichment PRM

Enrichment Method

N/A

Matrix

Cell Lysate

Submitting Laboratory

Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute

Submitting Lab PI

Amanda Paulovich, John Koomen, Steven A. Carr

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

SCIEX 5500 / Thermo Quantiva

Internal Standard

Stable Isotope Labeled Standard

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C and 15N V

LC

Dionex Ultimate 3000

Column Packing

C18 PepMap 100

Column Dimensions

75 micron ID x 25 cm length

Flow Rate

300 nL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y7 (1+) | 21.4 | 6.9 | 10.7 | 23.8 | 28.6 | 17.6 | 32 | 29.4 | 20.6 | 16 | 15 | 17 | | y16 (2+) | 25.4 | 7.2 | 10.2 | 32.3 | 26.4 | 17.9 | 41.1 | 27.4 | 20.6 | 16 | 15 | 17 | | sum | 21.8 | 6.7 | 10.5 | 26.6 | 27.6 | 17.7 | 34.4 | 28.4 | 20.6 | 16 | 15 | 17 |

Additional Resources and Comments

Assay Details for CPTAC-5810 Collapse assay details

Data source: Panorama

Official Gene Symbol

MAPK1

Peptide Modified Sequence

VADPDHDHTGFLTEY[+79.966331]VATR

Modification Type

Phospho (Y)

Protein - Site of Modification

N/A

Peptide - Site of Modification

3

Peptide Start

173

Peptide End

191

CPTAC ID

CPTAC-5810

Peptide Molecular Mass

2,222.9634

Species

Homo sapiens (Human)

Assay Type

Enrichment PRM

Enrichment Method

N/A

Matrix

Cell Lysate

Submitting Laboratory

Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute

Submitting Lab PI

Amanda Paulovich, John Koomen, Steven A. Carr

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

SCIEX 5500 / Thermo Quantiva

Internal Standard

Stable Isotope Labeled Standard

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C and 15N V

LC

Dionex Ultimate 3000

Column Packing

C18 PepMap 100

Column Dimensions

75 micron ID x 25 cm length

Flow Rate

300 nL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y7 (1+) | 6.3 | 7.9 | 8.6 | 16.4 | 21.8 | 16.7 | 17.6 | 23.2 | 18.8 | 16 | 15 | 16 | | y16 (2+) | 7.6 | 7.1 | 9.2 | 19.9 | 20.9 | 16.3 | 21.3 | 22.1 | 18.7 | 16 | 15 | 16 | | sum | 6.5 | 7.5 | 8.3 | 17.7 | 21.4 | 15.7 | 18.9 | 22.7 | 17.8 | 16 | 15 | 16 |

Additional Resources and Comments

Assay Details for CPTAC-5809 Collapse assay details

Data source: Panorama

Official Gene Symbol

MAPK1

Peptide Modified Sequence

VADPDHDHTGFLT[+79.966331]EYVATR

Modification Type

Phospho (ST)

Protein - Site of Modification

N/A

Peptide - Site of Modification

3

Peptide Start

173

Peptide End

191

CPTAC ID

CPTAC-5809

Peptide Molecular Mass

2,222.9634

Species

Homo sapiens (Human)

Assay Type

Enrichment PRM

Enrichment Method

N/A

Matrix

Cell Lysate

Submitting Laboratory

Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute

Submitting Lab PI

Amanda Paulovich, John Koomen, Steven A. Carr

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

SCIEX 5500 / Thermo Quantiva

Internal Standard

Stable Isotope Labeled Standard

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C and 15N V

LC

Dionex Ultimate 3000

Column Packing

C18 PepMap 100

Column Dimensions

75 micron ID x 25 cm length

Flow Rate

300 nL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y16 (2+) | 7.6 | 7.1 | 9.2 | 19.9 | 20.9 | 16.3 | 21.3 | 22.1 | 18.7 | 16 | 15 | 16 | | y7 (1+) | 6.3 | 7.9 | 8.6 | 16.4 | 21.8 | 16.7 | 17.6 | 23.2 | 18.8 | 16 | 15 | 16 | | sum | 6.5 | 7.5 | 8.3 | 17.7 | 21.4 | 15.7 | 18.9 | 22.7 | 17.8 | 16 | 15 | 16 |

Additional Resources and Comments

Assay Details for non-CPTAC-6107 Collapse assay details

Data source: Panorama

Official Gene Symbol

MAPK1

Peptide Sequence

ELIFEETAR

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

N/A

Peptide Start

345

Peptide End

353

CPTAC ID

non-CPTAC-6107

Peptide Molecular Mass

1,106.5608

Species

Homo sapiens (Human)

Assay Type

Direct PRM

Matrix

Bovine Serum Albumin

Submitting Laboratory

McGill University

Submitting Lab PI

Christoph H. Borchers, Gerald Batist

Publication

View Details (opens in a new window)

mTORC1-driven protein translation correlates with clinical benefit of capivasertib within a genetically preselected cohort of PIK3CA-altered tumours. Constance A Sobsey, Bjoern C Froehlich, Georgia Mitsa, Sahar Ibrahim, Robert Popp, Rene P Zahedi, Elza C de Bruin, Christoph H Borchers, Gerald Batist

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

Agilent 6495B QQQ-MS

Internal Standard

Standard Isotope labeled internal standard peptides with 13Cx, 15Ny (R+10 or K+8)

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C and 15N at C-terminus R

LC

Infinity 1290

Column Packing

commercial (Agilent ZORBAX Eclipse Plus C18)

Column Dimensions

4.6 mm × 150 mm, (5 µm)

Flow Rate

0.4 mL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | b2 (1+) | 11.7 | 5.1 | 4.8 | 7.1 | 5.8 | 6.1 | 13.7 | 7.7 | 7.8 | 16 | 16 | 17 | | y4 (1+) | 11.8 | 7.8 | 5.9 | 12.3 | 7.2 | 5.2 | 17 | 10.6 | 7.9 | 16 | 16 | 17 | | y6 (1+) | 6.1 | 6.1 | 7.2 | 9 | 7.8 | 7.5 | 10.9 | 9.9 | 10.4 | 16 | 16 | 17 | | y7 (1+) | 11.4 | 3.9 | 4.9 | 10.4 | 5.3 | 6 | 15.4 | 6.6 | 7.7 | 16 | 16 | 17 | | sum | 6.5 | 3 | 3.6 | 5.5 | 3.4 | 2.9 | 8.5 | 4.5 | 4.6 | 16 | 16 | 17 |

Additional Resources and Comments

Assay Details for non-CPTAC-5722 Collapse assay details

Data source: Panorama

Official Gene Symbol

MAPK1

Peptide Modified Sequence

VADPDHDHTGFLTEY[+79.966331]VATR

Modification Type

Phospho (Y)

Protein - Site of Modification

N/A

Peptide - Site of Modification

3

Peptide Start

173

Peptide End

191

CPTAC ID

non-CPTAC-5722

Peptide Molecular Mass

2,222.9634

Species

Homo sapiens (Human)

Assay Type

Enrichment PRM

Enrichment Method

N/A

Matrix

Cell Lysate

Submitting Laboratory

Moffitt Cancer Center

Submitting Lab PI

John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

Thermo Quantiva

Internal Standard

peptide

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C and 15N V

LC

Dionex Ultimate 3000 RSLCnano

Column Packing

Pepmap 100

Column Dimensions

75um x 25cm

Flow Rate

300 nL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y8 (1+) | 5.8 | 3.4 | 3.3 | 4.8 | 4.2 | 4.7 | 7.5 | 5.4 | 5.7 | 15 | 15 | 15 | | y7 (1+) | 3 | 2 | 2.5 | 4.5 | 3.2 | 4.1 | 5.4 | 3.8 | 4.8 | 15 | 15 | 15 | | y6 (1+) | 3.5 | 3.5 | 2.5 | 5.2 | 3.9 | 4.6 | 6.3 | 5.2 | 5.2 | 15 | 15 | 15 | | y5 (1+) | 4.8 | 2.7 | 2.7 | 5.4 | 3.5 | 4.2 | 7.2 | 4.4 | 5 | 15 | 15 | 15 | | b11 (2+) | 2.2 | 3 | 3.2 | 3 | 3.5 | 4.9 | 3.7 | 4.6 | 5.9 | 15 | 15 | 15 | | sum | 2.2 | 1.7 | 2.3 | 3.5 | 3 | 4.1 | 4.1 | 3.4 | 4.7 | 15 | 15 | 15 |

Additional Resources and Comments

Assay Details for non-CPTAC-5723 Collapse assay details

Data source: Panorama

Official Gene Symbol

MAPK1

Peptide Modified Sequence

VADPDHDHTGFLT[+79.966331]EY[+79.966331]VATR

Modification Type

Phospho (Y)

Protein - Site of Modification

N/A

Peptide - Site of Modification

3

Peptide Start

173

Peptide End

191

CPTAC ID

non-CPTAC-5723

Peptide Molecular Mass

2,302.9297

Species

Homo sapiens (Human)

Assay Type

Enrichment PRM

Enrichment Method

N/A

Matrix

Cell Lysate

Submitting Laboratory

Moffitt Cancer Center

Submitting Lab PI

John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

Thermo Quantiva

Internal Standard

peptide

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C and 15N V

LC

Dionex Ultimate 3000 RSLCnano

Column Packing

Pepmap 100

Column Dimensions

75um x 25cm

Flow Rate

300 nL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y16-98 (3+) | 7.3 | 5.2 | 3.3 | 7.7 | 5.4 | 3.9 | 10.6 | 7.5 | 5.1 | 15 | 15 | 15 | | y7-98 (1+) | 9.2 | 3.9 | 2.5 | 16.4 | 4.7 | 3.5 | 18.8 | 6.1 | 4.3 | 15 | 15 | 15 | | y5 (1+) | 8.7 | 2.8 | 3 | 9.7 | 2.8 | 3.5 | 13 | 4 | 4.6 | 15 | 15 | 15 | | sum | 3.7 | 2.3 | 1.7 | 7.5 | 2 | 2.1 | 8.4 | 3 | 2.7 | 15 | 15 | 15 |

Additional Resources and Comments

Assay Details for non-CPTAC-5721 Collapse assay details

Data source: Panorama

Official Gene Symbol

MAPK1

Peptide Modified Sequence

VADPDHDHTGFLT[+79.966331]EYVATR

Modification Type

Phospho (ST)

Protein - Site of Modification

N/A

Peptide - Site of Modification

3

Peptide Start

173

Peptide End

191

CPTAC ID

non-CPTAC-5721

Peptide Molecular Mass

2,222.9634

Species

Homo sapiens (Human)

Assay Type

Enrichment PRM

Enrichment Method

N/A

Matrix

Cell Lysate

Submitting Laboratory

Moffitt Cancer Center

Submitting Lab PI

John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

Thermo Quantiva

Internal Standard

peptide

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C and 15N V

LC

Dionex Ultimate 3000 RSLCnano

Column Packing

Pepmap 100

Column Dimensions

75um x 25cm

Flow Rate

300 nL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y10-98 (1+) | 9.3 | 4.3 | 5.5 | 11 | 6.8 | 6.1 | 14.4 | 8 | 8.2 | 15 | 15 | 15 | | y7-98 (1+) | 4.1 | 2.1 | 3.5 | 4 | 2 | 2.4 | 5.7 | 2.9 | 4.2 | 15 | 15 | 15 | | y6 (1+) | 3.9 | 3.7 | 2.3 | 4 | 4.5 | 3 | 5.6 | 5.8 | 3.8 | 15 | 15 | 15 | | y5 (1+) | 3.6 | 3.4 | 1.7 | 3.4 | 3.2 | 1.6 | 5 | 4.7 | 2.3 | 15 | 15 | 15 | | y4 (1+) | 4.3 | 3 | 1.4 | 4.2 | 3.1 | 1.9 | 6 | 4.3 | 2.4 | 15 | 15 | 15 | | sum | 3 | 1.3 | 1.2 | 2.6 | 1.5 | 1.5 | 4 | 2 | 1.9 | 15 | 15 | 15 |

Additional Resources and Comments

Assay Details for non-CPTAC-5406 Collapse assay details

Data source: Panorama

Official Gene Symbol

MAPK1

Peptide Sequence

VADPDHDHTGFLTEYVATR

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

N/A

Peptide Start

173

Peptide End

191

CPTAC ID

non-CPTAC-5406

Peptide Molecular Mass

2,142.9970

Species

Homo sapiens (Human)

Assay Type

Direct PRM

Matrix

Cell Lysate

Submitting Laboratory

Moffitt Cancer Center

Submitting Lab PI

John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

RSLCnano and Quantiva

Internal Standard

Stable Isotope Labeled Standard

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C and 15N V

LC

RSLCnano

Column Packing

C18 PepMap 100

Column Dimensions

75 micron ID x 25 cm length

Flow Rate

300 nl/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y4 (1+) | 18.2 | 5.4 | 2.5 | 19.1 | 4.3 | 3.5 | 26.4 | 6.9 | 4.3 | 15 | 15 | 15 | | y5 (1+) | 13 | 3.5 | 3.1 | 11.2 | 4.2 | 4.5 | 17.2 | 5.5 | 5.5 | 15 | 15 | 15 | | y6 (1+) | 19.9 | 6.2 | 2.1 | 21.3 | 6.3 | 3.2 | 29.1 | 8.8 | 3.8 | 15 | 15 | 15 | | y7 (1+) | 8.9 | 4.9 | 2 | 9.2 | 5.9 | 2.9 | 12.8 | 7.7 | 3.5 | 15 | 15 | 15 | | y10 (1+) | 40.9 | 8.3 | 4.7 | 39.6 | 10.3 | 4.9 | 56.9 | 13.2 | 6.8 | 15 | 15 | 15 | | sum | 4.8 | 1.7 | 1.3 | 6.8 | 2.4 | 2.8 | 8.3 | 2.9 | 3.1 | 15 | 15 | 15 |

Additional Resources and Comments

Assay Details for CPTAC-1541 Collapse assay details

Data source: Panorama

Official Gene Symbol

MAPK1

Peptide Sequence

VADPDHDHTGFLTEYVATR

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

N/A

Peptide Start

173

Peptide End

191

CPTAC ID

CPTAC-1541

Peptide Molecular Mass

2,142.9970

Species

Homo sapiens (Human)

Assay Type

Direct PRM

Matrix

Ovarian cancer tumor tissue lysate

Submitting Laboratory

Pacific Northwest National Laboratory

Submitting Lab PI

Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

TSQ Vantage

Internal Standard

peptide

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C and 15N V

LC

Waters nanoACQUITY (Part Number 176016000)

Column Packing

Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)

Column Dimensions

100 um x 100 mm

Flow Rate

0.5 uL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y10 (1+) | 30.8 | 11.8 | 6.1 | 27.8 | 11 | 11.9 | 41.5 | 16.1 | 13.4 | 15 | 15 | 15 | | y8 (1+) | 23.2 | 13.2 | 9.2 | 23.1 | 11.3 | 13.4 | 32.7 | 17.4 | 16.3 | 15 | 15 | 15 | | y7 (1+) | 16.6 | 5.8 | 3.7 | 15.4 | 6.1 | 9.1 | 22.6 | 8.4 | 9.8 | 15 | 15 | 15 | | y6 (1+) | 12.5 | 5.1 | 5.7 | 19.8 | 5.9 | 8.4 | 23.4 | 7.8 | 10.2 | 15 | 15 | 15 | | y5 (1+) | 10.2 | 5.6 | 4.6 | 17.6 | 6.4 | 7.6 | 20.3 | 8.5 | 8.9 | 15 | 15 | 15 | | sum | 6.9 | 5.1 | 1.8 | 11.5 | 4.9 | 7.4 | 13.4 | 7.1 | 7.6 | 15 | 15 | 15 |

Additional Resources and Comments

Assay Details for CPTAC-870 Collapse assay details

Data source: Panorama

Official Gene Symbol

MAPK1

Peptide Sequence

AAAAAAGAGPEMVR

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

N/A

Peptide Start

2

Peptide End

15

CPTAC ID

CPTAC-870

Peptide Molecular Mass

1,241.6187

Species

Homo sapiens (Human)

Assay Type

Direct PRM

Matrix

Pooled patient derived xenograft breast tumor digest

Submitting Laboratory

Washington University in St. Louis

Submitting Lab PI

Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

TripleTOF 5600+ (SCIEX)

Internal Standard

Peptide

Peptide Standard Purity

Crude

Peptide Standard Label Type

13C and 15N at C-terminus R

LC

2DPlus nano-LC (Eksigent)

Column Packing

ChromXP C18-CL, 3 µm, 120 Å

Column Dimensions

200 µm x 15 cm

Flow Rate

800 nL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y3 (1+) | 22.5 | 14.6 | 10.1 | 28.3 | 11.3 | 9.4 | 36.2 | 18.5 | 13.8 | 15 | 15 | 15 | | y5 (1+) | 13.4 | 9.9 | 11.9 | 14.1 | 11.2 | 16.3 | 19.5 | 14.9 | 20.2 | 15 | 15 | 15 | | y6 (1+) | 13.9 | 5 | 5.3 | 16 | 9 | 6.6 | 21.2 | 10.3 | 8.5 | 15 | 15 | 15 | | y7 (1+) | 10.2 | 9 | 11.5 | 17.4 | 10.8 | 12.4 | 20.2 | 14.1 | 16.9 | 15 | 15 | 15 | | y8 (1+) | 14.4 | 4.5 | 6.9 | 12.1 | 6.3 | 9.3 | 18.8 | 7.7 | 11.6 | 15 | 15 | 15 | | y9 (1+) | 8.3 | 4.6 | 8.1 | 9.6 | 9.1 | 8.8 | 12.7 | 10.2 | 12 | 15 | 15 | 15 | | y10 (1+) | 10 | 4.7 | 4.9 | 11.4 | 6.5 | 7.4 | 15.2 | 8 | 8.9 | 15 | 15 | 15 | | y11 (1+) | 18.1 | 12 | 10.5 | 21.3 | 13.2 | 11.2 | 28 | 17.8 | 15.4 | 15 | 15 | 15 | | b6 (2+) | 8.1 | 7.6 | 8.5 | 13.4 | 10.4 | 9.4 | 15.7 | 12.9 | 12.7 | 15 | 15 | 15 | | b5 (1+) | 22.7 | 13.7 | 15.1 | 26.3 | 16.1 | 17.6 | 34.7 | 21.1 | 23.2 | 15 | 15 | 15 | | b4 (1+) | 14.6 | 11 | 11.2 | 17.2 | 13 | 11.8 | 22.6 | 17 | 16.3 | 15 | 15 | 15 | | sum | 4 | 3 | 3.6 | 6.2 | 6 | 6.1 | 7.4 | 6.7 | 7.1 | 15 | 15 | 15 |

Additional Resources and Comments

Assay Details for CPTAC-871 Collapse assay details

Data source: Panorama

Official Gene Symbol

MAPK1

Peptide Sequence

ELIFEETAR

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

N/A

Peptide Start

345

Peptide End

353

CPTAC ID

CPTAC-871

Peptide Molecular Mass

1,106.5608

Species

Homo sapiens (Human)

Assay Type

Direct PRM

Matrix

Pooled patient derived xenograft breast tumor digest

Submitting Laboratory

Washington University in St. Louis

Submitting Lab PI

Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

TripleTOF 5600+ (SCIEX)

Internal Standard

Peptide

Peptide Standard Purity

Crude

Peptide Standard Label Type

13C and 15N at C-terminus R

LC

2DPlus nano-LC (Eksigent)

Column Packing

ChromXP C18-CL, 3 µm, 120 Å

Column Dimensions

200 µm x 15 cm

Flow Rate

800 nL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y3 (1+) | 15 | 6.7 | 6.3 | 14 | 7.2 | 7.1 | 20.5 | 9.8 | 9.5 | 15 | 15 | 15 | | y4 (1+) | 8.3 | 5.1 | 4.8 | 8.4 | 7.1 | 6.2 | 11.8 | 8.7 | 7.8 | 15 | 15 | 15 | | y5 (1+) | 6.2 | 3.5 | 2.3 | 8 | 4.8 | 4.4 | 10.1 | 5.9 | 5 | 15 | 15 | 15 | | y6 (1+) | 4.6 | 1.8 | 2.6 | 6.9 | 3.3 | 3.1 | 8.3 | 3.8 | 4 | 15 | 15 | 15 | | y7 (1+) | 5 | 5.7 | 4.2 | 7.3 | 4.7 | 3.9 | 8.8 | 7.4 | 5.7 | 15 | 15 | 15 | | sum | 3.8 | 1.5 | 1.7 | 5.9 | 3.5 | 2.8 | 7 | 3.8 | 3.3 | 15 | 15 | 15 |

Additional Resources and Comments

Assay Details for CPTAC-3200 Collapse assay details

Data source: Panorama

Official Gene Symbol

MAPK1

Peptide Sequence

VADPDHDHTGFLTEYVATR

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

N/A

Peptide Start

173

Peptide End

191

CPTAC ID

CPTAC-3200

Peptide Molecular Mass

2,142.9970

Species

Homo Sapiens

Assay Type

Direct PRM

Matrix

Tumor Digest

Submitting Laboratory

Washington University in St. Louis

Submitting Lab PI

Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

ThermoFisher, Q-Exactive

Internal Standard

25 fmol on column

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C and 15N V

LC

Easy-nLC 1000 Thermo Scientific

Column Packing

C18

Column Dimensions

75 µm x 50 cm

Flow Rate

300 nL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y4 (1+) | 28.5 | 6.8 | 7.9 | 42.5 | 6.5 | 7.9 | 51.2 | 9.4 | 11.2 | 15 | 15 | 15 | | b11 (2+) | 45.3 | 10.4 | 5.6 | 47.6 | 6.9 | 5.8 | 65.7 | 12.5 | 8.1 | 15 | 15 | 15 | | y3 (1+) | 8.3 | 8.6 | 6.3 | 12.1 | 8.2 | 6.8 | 14.7 | 11.9 | 9.3 | 15 | 15 | 15 | | sum | 13.1 | 8.1 | 6.5 | 21.7 | 7.1 | 6.8 | 25.3 | 10.8 | 9.4 | 15 | 15 | 15 |

Additional Resources and Comments