MAPK3, CPTAC-880 - CPTAC Assay Portal (original) (raw)
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.
Protein Sequence hover to view complete sequence
CPTAC-5760: View additional ALDLLDR data
non-CPTAC-5559: View additional ALDLLDR data
non-CPTAC-5412: View additional GQPFDVGPR data
non-CPTAC-5415: View additional NYLQSLPSK data
| 10 | 20 | 30 | 40 | 50 |
|---|---|---|---|---|
| MAAAAAQGGG | GGEPRRTEGV | GPGVPGEVEM | VKGQPFDVGP | RYTQLQYIGE |
| 60 | 70 | 80 | 90 | 100 |
| GAYGMVSSAY | DHVRKTRVAI | KKISPFEHQT | YCQRTLREIQ | ILLRFRHENV |
| 110 | 120 | 130 | 140 | 150 |
| IGIRDILRAS | TLEAMRDVYI | VQDLMETDLY | KLLKSQQLSN | DHICYFLYQI |
| 160 | 170 | 180 | 190 | 200 |
| LRGLKYIHSA | NVLHRDLKPS | NLLINTTCDL | KICDFGLARI | ADPEHDHTGF |
| 210 | 220 | 230 | 240 | 250 |
| LTEYVATRWY | RAPEIMLNSK | GYTKSIDIWS | VGCILAEMLS | NRPIFPGKHY |
| 260 | 270 | 280 | 290 | 300 |
| LDQLNHILGI | LGSPSQEDLN | CIINMKARNY | LQSLPSKTKV | AWAKLFPKSD |
| 310 | 320 | 330 | 340 | 350 |
| SKALDLLDRM | LTFNPNKRIT | VEEALAHPYL | EQYYDPTDEP | VAEEPFTFAM |
| 360 | 370 | 379 | ||
| ELDDLPKERL | KELIFQETAR | FQPGVLEAP |
Data source: UniProt
Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs
Uniprot Database Entry PhosphoSitePlus ®
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to view detailed assay information below
All other points link out to UniProt
Phosphorylation Acetylation Ubiquitylation Other
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Assay Details for CPTAC-1357 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAPK3
Peptide Modified Sequence
IADPEHDHTGFLT[+80.0]EY[+80.0]VATR
Modification Type
Phospho (ST), Phospho (Y)
Protein - Site of Modification
202, 204
Peptide - Site of Modification
13, 15
Peptide Start
190
Peptide End
208
CPTAC ID
CPTAC-1357
Peptide Molecular Mass
2,330.9610
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
digested cell lysate
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr
Publication
View Details (opens in a new window)
Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. Reduced-representation Phosphosignatures Measured by Quantitative Targeted MS Capture Cellular States and Enable Large-scale Comparison of Drug-induced Phenotypes. Abelin JG1, Patel J1, Lu X1, Feeney CM1, Fagbami L1, Creech AL1, Hu R1, Lam D1, Davison D1, Pino L1, Qiao JW1, Kuhn E1, Officer A1, Li J2, Abbatiello S1, Subramanian A1, Sidman R2, Snyder E3, Carr SA1, Jaffe JD4.
View Details (opens in a new window)
Mol Cell Proteomics. 2014 Jul;13(7):1690-704. doi: 10.1074/mcp.M113.036392. Epub 2014 Apr 9. Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mertins P1, Yang F2, Liu T2, Mani DR3, Petyuk VA2, Gillette MA3, Clauser KR3, Qiao JW3, Gritsenko MA2, Moore RJ2, Levine DA4, Townsend R5, Erdmann-Gilmore P5, Snider JE5, Davies SR5, Ruggles KV6, Fenyo D6, Kitchens RT5, Li S5, Olvera N4, Dao F4, Rodriguez H7, Chan DW8, Liebler D9, White F10, Rodland KD2, Mills GB11, Smith RD2, Paulovich AG12, Ellis M5, Carr SA1
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
Quantiva
Internal Standard
light stable isotope peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N V
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV
| n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y16-98 (2+) | 10.6 | 6 | 7.2 | 10 | 12.5 | 10.3 | 14.6 | 13.9 | 12.6 | 15 | 15 | 15 |
| y5 (1+) | 6.6 | 7.8 | 6.5 | 7.8 | 11.3 | 10.4 | 10.2 | 13.7 | 12.3 | 15 | 15 | 15 |
| y7-98 (1+) | 5.6 | 5.7 | 5 | 6.9 | 11.5 | 10.5 | 8.9 | 12.8 | 11.6 | 15 | 15 | 15 |
| sum | 5.6 | 5.5 | 4.3 | 6.9 | 11.1 | 9.8 | 8.9 | 12.4 | 10.7 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-1358 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAPK3
Peptide Modified Sequence
IADPEHDHTGFLT[+80.0]EYVATR
Modification Type
Phospho (ST)
Protein - Site of Modification
202
Peptide - Site of Modification
13
Peptide Start
190
Peptide End
208
CPTAC ID
CPTAC-1358
Peptide Molecular Mass
2,250.9947
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
digested cell lysate
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr
Publication
View Details (opens in a new window)
Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. Reduced-representation Phosphosignatures Measured by Quantitative Targeted MS Capture Cellular States and Enable Large-scale Comparison of Drug-induced Phenotypes. Abelin JG1, Patel J1, Lu X1, Feeney CM1, Fagbami L1, Creech AL1, Hu R1, Lam D1, Davison D1, Pino L1, Qiao JW1, Kuhn E1, Officer A1, Li J2, Abbatiello S1, Subramanian A1, Sidman R2, Snyder E3, Carr SA1, Jaffe JD4.
View Details (opens in a new window)
Mol Cell Proteomics. 2014 Jul;13(7):1690-704. doi: 10.1074/mcp.M113.036392. Epub 2014 Apr 9. Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mertins P1, Yang F2, Liu T2, Mani DR3, Petyuk VA2, Gillette MA3, Clauser KR3, Qiao JW3, Gritsenko MA2, Moore RJ2, Levine DA4, Townsend R5, Erdmann-Gilmore P5, Snider JE5, Davies SR5, Ruggles KV6, Fenyo D6, Kitchens RT5, Li S5, Olvera N4, Dao F4, Rodriguez H7, Chan DW8, Liebler D9, White F10, Rodland KD2, Mills GB11, Smith RD2, Paulovich AG12, Ellis M5, Carr SA1
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
Quantiva
Internal Standard
light stable isotope peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N V
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y16-98 (2+) | 17.5 | 15.7 | 10.4 | 26.3 | 18.8 | 18.7 | 31.6 | 24.5 | 21.4 | 15 | 14 | 15 |
| y6 (1+) | 8.9 | 10.9 | 12.9 | 15.2 | 12.1 | 13.5 | 17.6 | 16.3 | 18.7 | 14 | 14 | 15 |
| y7-98 (1+) | 11.6 | 9.4 | 3.8 | 16.1 | 12.8 | 10.5 | 19.8 | 15.9 | 11.2 | 15 | 15 | 15 |
| sum | 6.7 | 10.1 | 5.1 | 12.1 | 13 | 7.9 | 13.8 | 16.5 | 9.4 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-1359 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAPK3
Peptide Modified Sequence
IADPEHDHTGFLTEY[+80.0]VATR
Modification Type
Phospho (Y)
Protein - Site of Modification
204
Peptide - Site of Modification
15
Peptide Start
190
Peptide End
208
CPTAC ID
CPTAC-1359
Peptide Molecular Mass
2,250.9947
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
digested cell lysate
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr
Publication
View Details (opens in a new window)
Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. Reduced-representation Phosphosignatures Measured by Quantitative Targeted MS Capture Cellular States and Enable Large-scale Comparison of Drug-induced Phenotypes. Abelin JG1, Patel J1, Lu X1, Feeney CM1, Fagbami L1, Creech AL1, Hu R1, Lam D1, Davison D1, Pino L1, Qiao JW1, Kuhn E1, Officer A1, Li J2, Abbatiello S1, Subramanian A1, Sidman R2, Snyder E3, Carr SA1, Jaffe JD4.
View Details (opens in a new window)
Mol Cell Proteomics. 2014 Jul;13(7):1690-704. doi: 10.1074/mcp.M113.036392. Epub 2014 Apr 9. Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mertins P1, Yang F2, Liu T2, Mani DR3, Petyuk VA2, Gillette MA3, Clauser KR3, Qiao JW3, Gritsenko MA2, Moore RJ2, Levine DA4, Townsend R5, Erdmann-Gilmore P5, Snider JE5, Davies SR5, Ruggles KV6, Fenyo D6, Kitchens RT5, Li S5, Olvera N4, Dao F4, Rodriguez H7, Chan DW8, Liebler D9, White F10, Rodland KD2, Mills GB11, Smith RD2, Paulovich AG12, Ellis M5, Carr SA1
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
Quantiva
Internal Standard
light stable isotope peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N V
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y16 (2+) | 4.8 | 5.5 | 7.4 | 6.3 | 5.9 | 11.1 | 7.9 | 8.1 | 13.3 | 15 | 15 | 15 |
| y5 (1+) | 5.3 | 9.2 | 7 | 5.8 | 8.1 | 9 | 7.9 | 12.3 | 11.4 | 15 | 15 | 15 |
| y7 (1+) | 5.2 | 7.3 | 5.4 | 3.7 | 7.4 | 7.4 | 6.4 | 10.4 | 9.2 | 15 | 15 | 15 |
| sum | 4.3 | 6.3 | 5.8 | 4.3 | 6.2 | 8.3 | 6.1 | 8.8 | 10.1 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for non-CPTAC-6254 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAPK3
Peptide Modified Sequence
IADPEHDHTGFLT[+79.966331]EY[+79.966331]VATR
Modification Type
Phospho (Y)
Protein - Site of Modification
N/A
Peptide - Site of Modification
8
Peptide Start
190
Peptide End
208
CPTAC ID
non-CPTAC-6254
Peptide Molecular Mass
2,330.9610
Species
Human
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
SKBR3 cells
Submitting Laboratory
Cell Signaling Technology
Submitting Lab PI
Yiying Zhu
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
Orbitrap Q Exactive (Thermo)
Internal Standard
Heavy isotope-labeled peptides
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N V
LC
EASY-nLC II (Thermo)
Column Packing
self-packed
Column Dimensions
ID 100um, length 15cm
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y7-98 (1+) | 4.2 | 3.5 | 4 | 13.1 | 4.3 | 5 | 13.8 | 5.5 | 6.4 | 15 | 15 | 15 |
| y6 (1+) | 8.1 | 4.1 | 4.2 | 13 | 6 | 5.8 | 15.3 | 7.3 | 7.2 | 15 | 15 | 15 |
| y8-98 (1+) | 4.2 | 3.7 | 5.2 | 13.8 | 4.2 | 4.9 | 14.4 | 5.6 | 7.1 | 15 | 15 | 15 |
| y16-98 (2+) | 6.5 | 3.1 | 4 | 16.4 | 3.8 | 5.2 | 17.6 | 4.9 | 6.6 | 15 | 15 | 15 |
| y16 (2+) | 12.7 | 3.5 | 4.6 | 19.9 | 4 | 4.4 | 23.6 | 5.3 | 6.4 | 15 | 15 | 15 |
| y15 (2+) | 8.4 | 3.1 | 4.5 | 18.8 | 3.9 | 6 | 20.6 | 5 | 7.5 | 15 | 15 | 15 |
| sum | 4.2 | 3 | 4 | 14.7 | 3.8 | 4.9 | 15.3 | 4.8 | 6.3 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-5760 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAPK3
Peptide Sequence
ALDLLDR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
303
Peptide End
309
CPTAC ID
CPTAC-5760
Peptide Molecular Mass
814.4549
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI
Amanda Paulovich, John Koomen, Steven A. Carr
Publication
View Details (opens in a new window)
Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
SCIEX 5500 / Thermo Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Dionex Ultimate 3000
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y5 (1+) | 25.9 | 8.4 | 7.9 | 28.5 | 22.4 | 17 | 38.5 | 23.9 | 18.7 | 15 | 15 | 15 |
| y4 (1+) | 27.2 | 18.5 | 7 | 28.1 | 31.8 | 16.4 | 39.1 | 36.8 | 17.8 | 15 | 15 | 15 |
| sum | 21 | 8.9 | 7.6 | 24.6 | 22.9 | 16.8 | 32.3 | 24.6 | 18.4 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-5816 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAPK3
Peptide Modified Sequence
IADPEHDHTGFLT[+79.966331]EYVATR
Modification Type
Phospho (ST)
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
190
Peptide End
208
CPTAC ID
CPTAC-5816
Peptide Molecular Mass
2,250.9947
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI
Amanda Paulovich, John Koomen, Steven A. Carr
Publication
View Details (opens in a new window)
Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
SCIEX 5500 / Thermo Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N V
LC
Dionex Ultimate 3000
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y7 (1+) | 6 | 9.3 | 8.2 | 18.5 | 21.6 | 15.5 | 19.4 | 23.5 | 17.5 | 16 | 15 | 16 |
| y16 (2+) | 6.6 | 9 | 11.3 | 19.2 | 21 | 15.5 | 20.3 | 22.8 | 19.2 | 16 | 15 | 16 |
| sum | 5.6 | 9.1 | 9.4 | 18.7 | 21.3 | 15.2 | 19.5 | 23.2 | 17.9 | 16 | 15 | 16 |
Additional Resources and Comments
Assay Details for CPTAC-5815 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAPK3
Peptide Modified Sequence
IADPEHDHTGFLTEY[+79.966331]VATR
Modification Type
Phospho (Y)
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
190
Peptide End
208
CPTAC ID
CPTAC-5815
Peptide Molecular Mass
2,250.9947
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI
Amanda Paulovich, John Koomen, Steven A. Carr
Publication
View Details (opens in a new window)
Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
SCIEX 5500 / Thermo Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N V
LC
Dionex Ultimate 3000
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y16 (2+) | 6.6 | 9 | 11.3 | 19.2 | 21 | 15.5 | 20.3 | 22.8 | 19.2 | 16 | 15 | 16 |
| y7 (1+) | 6 | 9.3 | 8.2 | 18.5 | 21.6 | 15.5 | 19.4 | 23.5 | 17.5 | 16 | 15 | 16 |
| sum | 5.6 | 9.1 | 9.4 | 18.7 | 21.3 | 15.2 | 19.5 | 23.2 | 17.9 | 16 | 15 | 16 |
Additional Resources and Comments
Assay Details for CPTAC-5814 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAPK3
Peptide Modified Sequence
IADPEHDHTGFLT[+79.966331]EY[+79.966331]VATR
Modification Type
Phospho (Y)
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
190
Peptide End
208
CPTAC ID
CPTAC-5814
Peptide Molecular Mass
2,330.9610
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI
Amanda Paulovich, John Koomen, Steven A. Carr
Publication
View Details (opens in a new window)
Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
SCIEX 5500 / Thermo Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N V
LC
Dionex Ultimate 3000
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y15 (2+) | 14.4 | 10.6 | 17.8 | 18.1 | 19.6 | 22.5 | 23.1 | 22.3 | 28.7 | 16 | 15 | 16 |
| y15 (3+) | 11.9 | 13.5 | 14.1 | 21.9 | 21.9 | 19.6 | 24.9 | 25.7 | 24.1 | 16 | 15 | 16 |
| sum | 12.5 | 12 | 13.1 | 19.7 | 20.9 | 18.8 | 23.3 | 24.1 | 22.9 | 16 | 15 | 16 |
Additional Resources and Comments
Assay Details for CPTAC-5813 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAPK3
Peptide Sequence
IADPEHDHTGFLTEYVATR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
190
Peptide End
208
CPTAC ID
CPTAC-5813
Peptide Molecular Mass
2,171.0283
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI
Amanda Paulovich, John Koomen, Steven A. Carr
Publication
View Details (opens in a new window)
Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
SCIEX 5500 / Thermo Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N V
LC
Dionex Ultimate 3000
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y5 (1+) | 10.7 | 4.5 | 10.9 | 13.9 | 22.9 | 17 | 17.5 | 23.3 | 20.2 | 16 | 15 | 17 |
| y6 (1+) | 21.8 | 4.7 | 11.8 | 25.2 | 25.6 | 18.2 | 33.3 | 26 | 21.7 | 16 | 15 | 17 |
| y7 (1+) | 19.4 | 5.2 | 10.6 | 28.2 | 28.8 | 17.5 | 34.2 | 29.3 | 20.5 | 16 | 15 | 17 |
| sum | 13.9 | 4.5 | 10.9 | 20.7 | 26.2 | 17.5 | 24.9 | 26.6 | 20.6 | 16 | 15 | 17 |
Additional Resources and Comments
Assay Details for non-CPTAC-5559 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAPK3
Peptide Sequence
ALDLLDR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
6
Peptide Start
303
Peptide End
309
CPTAC ID
non-CPTAC-5559
Peptide Molecular Mass
814.4549
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
Cell Line
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen
Publication
View Details (opens in a new window)
Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
RSLCnano and Quantiva
Internal Standard
SIS Peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
RSLCnano
Column Packing
C18 PepMap100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y4 (1+) | 25.4 | 8.6 | 3.5 | 26 | 26.4 | 13.5 | 36.3 | 27.8 | 13.9 | 14 | 14 | 14 |
| y5 (1+) | 24 | 7.2 | 4.9 | 26.1 | 23.9 | 13.6 | 35.5 | 25 | 14.5 | 14 | 14 | 14 |
| y6 (1+) | 79.3 | 16.3 | 10.5 | 98.3 | 32.3 | 17.9 | 126.3 | 36.2 | 20.8 | 14 | 14 | 14 |
| sum | 20.6 | 6.1 | 4.3 | 23.4 | 24.2 | 13.6 | 31.2 | 25 | 14.3 | 14 | 14 | 14 |
Additional Resources and Comments
Assay Details for non-CPTAC-5412 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAPK3
Peptide Sequence
GQPFDVGPR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
33
Peptide End
41
CPTAC ID
non-CPTAC-5412
Peptide Molecular Mass
971.4825
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen
Publication
View Details (opens in a new window)
Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
RSLCnano and Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
RSLCnano
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nl/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y4 (1+) | 3 | 2.8 | 1.4 | 3.8 | 3.2 | 2.5 | 4.8 | 4.3 | 2.9 | 15 | 15 | 15 |
| y5 (1+) | 7.2 | 3.7 | 1.3 | 6.9 | 3.9 | 2.1 | 10 | 5.4 | 2.5 | 15 | 15 | 15 |
| y6 (1+) | 7.8 | 2 | 1.4 | 7.9 | 3.2 | 2.5 | 11.1 | 3.8 | 2.9 | 15 | 15 | 15 |
| y7 (1+) | 2.7 | 1.8 | 1 | 2.8 | 2.2 | 2 | 3.9 | 2.8 | 2.2 | 15 | 15 | 15 |
| sum | 2.2 | 1.8 | 0.7 | 2.6 | 2.4 | 2 | 3.4 | 3 | 2.1 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for non-CPTAC-5414 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAPK3
Peptide Sequence
IADPEHDHTGFLTEYVATR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
190
Peptide End
208
CPTAC ID
non-CPTAC-5414
Peptide Molecular Mass
2,171.0283
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen
Publication
View Details (opens in a new window)
Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
RSLCnano and Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N V
LC
RSLCnano
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nl/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y16 (2+) | 10.4 | 6 | 3 | 14.5 | 7.4 | 5.6 | 17.8 | 9.5 | 6.4 | 15 | 15 | 15 |
| y4 (1+) | 15.1 | 8 | 6 | 16.3 | 7.8 | 6.5 | 22.2 | 11.2 | 8.8 | 15 | 15 | 15 |
| y5 (1+) | 14.1 | 5.3 | 2.8 | 15.1 | 6.7 | 3.7 | 20.7 | 8.5 | 4.6 | 15 | 15 | 15 |
| y6 (1+) | 18.2 | 5.2 | 5 | 12.3 | 4 | 4.6 | 22 | 6.6 | 6.8 | 15 | 15 | 15 |
| y7 (1+) | 12.3 | 6 | 3 | 12.7 | 5.7 | 3.8 | 17.7 | 8.3 | 4.8 | 15 | 15 | 15 |
| sum | 6.5 | 2.4 | 1.7 | 5.5 | 3.5 | 2.3 | 8.5 | 4.2 | 2.9 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for non-CPTAC-5724 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAPK3
Peptide Modified Sequence
IADPEHDHTGFLT[+79.966331]EYVATR
Modification Type
Phospho (ST)
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
190
Peptide End
208
CPTAC ID
non-CPTAC-5724
Peptide Molecular Mass
2,250.9947
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen
Publication
View Details (opens in a new window)
Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
Thermo Quantiva
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N V
LC
Dionex Ultimate 3000 RSLCnano
Column Packing
Pepmap 100
Column Dimensions
75um x 25cm
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y7-98 (1+) | 3.9 | 2.4 | 3.8 | 5.7 | 3.3 | 3.2 | 6.9 | 4.1 | 5 | 15 | 15 | 15 |
| y5 (1+) | 6.6 | 2.7 | 1.9 | 7.9 | 3.4 | 2.2 | 10.3 | 4.3 | 2.9 | 15 | 15 | 15 |
| y4 (1+) | 2.7 | 3.7 | 2 | 3.6 | 3.3 | 2.2 | 4.5 | 5 | 3 | 15 | 15 | 15 |
| y16-98 (2+) | 6.7 | 3.3 | 4 | 9.1 | 4 | 4 | 11.3 | 5.2 | 5.7 | 15 | 15 | 15 |
| b11 (2+) | 10.2 | 6.3 | 3.4 | 10.3 | 6.5 | 5.1 | 14.5 | 9.1 | 6.1 | 15 | 15 | 15 |
| sum | 2.6 | 1.7 | 1.9 | 4.4 | 1.9 | 1.9 | 5.1 | 2.5 | 2.7 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for non-CPTAC-5725 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAPK3
Peptide Modified Sequence
IADPEHDHTGFLTEY[+79.966331]VATR
Modification Type
Phospho (Y)
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
190
Peptide End
208
CPTAC ID
non-CPTAC-5725
Peptide Molecular Mass
2,250.9947
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen
Publication
View Details (opens in a new window)
Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
Thermo Quantiva
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N V
LC
Dionex Ultimate 3000 RSLCnano
Column Packing
Pepmap 100
Column Dimensions
75um x 25cm
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y7 (1+) | 3 | 1.7 | 1.8 | 3.6 | 3.4 | 2.7 | 4.7 | 3.8 | 3.2 | 15 | 15 | 15 |
| y6 (1+) | 2.9 | 2.7 | 3.1 | 4.1 | 3.7 | 3.5 | 5 | 4.6 | 4.7 | 15 | 15 | 15 |
| y5 (1+) | 3.7 | 3.5 | 2.6 | 4.6 | 3.4 | 3.8 | 5.9 | 4.9 | 4.6 | 15 | 15 | 15 |
| y16 (2+) | 4 | 1.9 | 2.4 | 3.8 | 3.1 | 3.4 | 5.5 | 3.6 | 4.2 | 15 | 15 | 15 |
| b11 (2+) | 5.8 | 2.9 | 2.2 | 6.6 | 3.8 | 3.8 | 8.8 | 4.8 | 4.4 | 15 | 15 | 15 |
| sum | 2.3 | 1.4 | 1.7 | 3.1 | 2.6 | 2.8 | 3.9 | 3 | 3.3 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for non-CPTAC-5726 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAPK3
Peptide Modified Sequence
IADPEHDHTGFLT[+79.966331]EY[+79.966331]VATR
Modification Type
Phospho (Y)
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
190
Peptide End
208
CPTAC ID
non-CPTAC-5726
Peptide Molecular Mass
2,330.9610
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen
Publication
View Details (opens in a new window)
Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
Thermo Quantiva
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N V
LC
Dionex Ultimate 3000 RSLCnano
Column Packing
Pepmap 100
Column Dimensions
75um x 25cm
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| b3 (1+) | 6.4 | 3.5 | 2.9 | 6.8 | 3.8 | 2.7 | 9.3 | 5.2 | 4 | 15 | 15 | 15 |
| y7-98 (1+) | 4.7 | 2.6 | 2.4 | 4.8 | 3 | 2.3 | 6.7 | 4 | 3.3 | 15 | 15 | 15 |
| y5 (1+) | 8.7 | 3.3 | 3.1 | 7.3 | 3.4 | 3.3 | 11.4 | 4.7 | 4.5 | 15 | 15 | 15 |
| y16-98 (3+) | 8.8 | 3.3 | 3.9 | 10.2 | 4.1 | 4.1 | 13.5 | 5.3 | 5.7 | 15 | 15 | 15 |
| y15 (3+) | 14.7 | 5.1 | 5.3 | 16.8 | 5.7 | 6.4 | 22.3 | 7.6 | 8.3 | 15 | 15 | 15 |
| sum | 4.4 | 1.7 | 1.7 | 4.8 | 1.4 | 1.9 | 6.5 | 2.2 | 2.5 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for non-CPTAC-5415 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAPK3
Peptide Sequence
NYLQSLPSK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
279
Peptide End
287
CPTAC ID
non-CPTAC-5415
Peptide Molecular Mass
1,048.5553
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen
Publication
View Details (opens in a new window)
Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
RSLCnano and Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
RSLCnano
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nl/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y3 (1+) | 3.5 | 2.5 | 1.5 | 6.1 | 2.5 | 2.3 | 7 | 3.5 | 2.7 | 15 | 15 | 15 |
| y5 (1+) | 10 | 8.3 | 13.5 | 10.4 | 17.2 | 20.3 | 14.4 | 19.1 | 24.4 | 15 | 15 | 15 |
| y6 (1+) | 19 | 3.4 | 2.4 | 20 | 3.4 | 2.5 | 27.6 | 4.8 | 3.5 | 15 | 15 | 15 |
| y7 (1+) | 3.8 | 2.2 | 1.4 | 3.8 | 2.2 | 2 | 5.4 | 3.1 | 2.4 | 15 | 15 | 15 |
| sum | 2.6 | 2.2 | 1.9 | 3.1 | 3.4 | 3.2 | 4 | 4 | 3.7 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-1542 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAPK3
Peptide Sequence
IADPEHDHTGFLTEYVATR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
190
Peptide End
208
CPTAC ID
CPTAC-1542
Peptide Molecular Mass
2,171.0283
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N V
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y7 (1+) | 18.8 | 3.6 | 3.2 | 22.3 | 4.1 | 7.1 | 29.2 | 5.5 | 7.8 | 15 | 15 | 15 |
| y6 (1+) | 22.3 | 6.3 | 5.7 | 19.3 | 6.5 | 10.6 | 29.5 | 9.1 | 12 | 15 | 15 | 15 |
| y16 (2+) | 32.5 | 5.3 | 7.3 | 34.4 | 5.4 | 12 | 47.3 | 7.6 | 14 | 15 | 15 | 15 |
| sum | 8.6 | 2.8 | 2.9 | 13 | 3.9 | 8.6 | 15.6 | 4.8 | 9.1 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-880 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAPK3
Peptide Modified Sequence
IADPEHDHTGFLTEY[+80.0]VATR
Modification Type
Phospho (Y)
Protein - Site of Modification
204
Peptide - Site of Modification
15
Peptide Start
190
Peptide End
208
CPTAC ID
CPTAC-880
Peptide Molecular Mass
2,250.9947
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N V
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y3 (1+) | 20.5 | 13.9 | 18.9 | 26 | 19.6 | 28.7 | 33.1 | 24 | 34.4 | 15 | 15 | 15 |
| y4 (1+) | 24.9 | 20.3 | 13.5 | 31.5 | 22.4 | 13.9 | 40.2 | 30.2 | 19.4 | 15 | 15 | 15 |
| y5 (1+) | 21.7 | 13.9 | 14.4 | 21.1 | 19.4 | 15.7 | 30.3 | 23.9 | 21.3 | 15 | 15 | 15 |
| y6 (1+) | 35.4 | 13.1 | 10 | 32.2 | 15 | 11.1 | 47.9 | 19.9 | 14.9 | 15 | 15 | 15 |
| y7 (1+) | 12.3 | 7 | 7 | 17.7 | 7.1 | 6 | 21.6 | 10 | 9.2 | 15 | 15 | 15 |
| y8 (1+) | 33.4 | 11.3 | 21.1 | 23.3 | 12.3 | 18.9 | 40.7 | 16.7 | 28.3 | 15 | 15 | 15 |
| y9 (1+) | 63.5 | 21.3 | 19.8 | 52.7 | 26 | 18.2 | 82.5 | 33.6 | 26.9 | 15 | 15 | 15 |
| y10 (1+) | 26.8 | 18.5 | 16 | 26.7 | 19.2 | 21.3 | 37.8 | 26.7 | 26.6 | 15 | 15 | 15 |
| y11 (1+) | 51.5 | 18.8 | 25.3 | 55.8 | 24.8 | 24.2 | 75.9 | 31.1 | 35 | 15 | 15 | 15 |
| y12 (1+) | 63.5 | 19.8 | 25.4 | 55.8 | 26.9 | 29.4 | 84.5 | 33.4 | 38.9 | 15 | 15 | 15 |
| b13 (1+) | 141.4 | 121.9 | 93.7 | 74 | 100.5 | 126.3 | 159.6 | 158 | 157.3 | 8 | 8 | 10 |
| b12 (1+) | 64.9 | 43.3 | 59.8 | 61.6 | 53.4 | 55.2 | 89.5 | 68.7 | 81.4 | 15 | 15 | 15 |
| b11 (1+) | 37 | 26.1 | 23.6 | 58 | 28.3 | 23.4 | 68.8 | 38.5 | 33.2 | 15 | 15 | 15 |
| b10 (1+) | 71.4 | 48 | 26.2 | 41.5 | 49.6 | 31 | 82.6 | 69 | 40.6 | 15 | 15 | 15 |
| b9 (1+) | 47.2 | 41.5 | 45.6 | 53.1 | 39.8 | 54.8 | 71 | 57.5 | 71.3 | 14 | 15 | 15 |
| b8 (1+) | 43.1 | 46.6 | 45.3 | 54.4 | 49.2 | 42.2 | 69.4 | 67.8 | 61.9 | 15 | 15 | 15 |
| b7 (1+) | 45.3 | 25.6 | 18.9 | 43.5 | 27.8 | 31.4 | 62.8 | 37.8 | 36.6 | 15 | 15 | 15 |
| b6 (1+) | 70.4 | 67.2 | 39.3 | 65.7 | 80.2 | 72.1 | 96.3 | 104.6 | 82.1 | 15 | 15 | 15 |
| b5 (1+) | 46.1 | 72.4 | 49.7 | 60.2 | 76.8 | 40.4 | 75.8 | 105.5 | 64 | 15 | 15 | 15 |
| b4 (1+) | 53.6 | 47.7 | 48.4 | 65 | 51 | 79.1 | 84.2 | 69.8 | 92.7 | 15 | 15 | 15 |
| b3 (1+) | 23.9 | 25.1 | 23 | 31.8 | 34.7 | 44.1 | 39.8 | 42.8 | 49.7 | 15 | 15 | 15 |
| sum | 11.5 | 5.5 | 4.7 | 15.1 | 5.7 | 5.6 | 19 | 7.9 | 7.3 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-3195 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAPK3
Peptide Sequence
IADPEHDHTGFLTEYVATR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
190
Peptide End
208
CPTAC ID
CPTAC-3195
Peptide Molecular Mass
2,171.0283
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N V
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y6 (1+) | 26.7 | 8 | 5.1 | 27.7 | 16.4 | 10.8 | 38.5 | 18.2 | 11.9 | 15 | 15 | 15 |
| b11 (2+) | 23.6 | 7.2 | 7.6 | 24.4 | 13.6 | 13.1 | 33.9 | 15.4 | 15.1 | 15 | 15 | 15 |
| y4 (1+) | 8.1 | 6.8 | 4.5 | 10 | 13.5 | 12.5 | 12.9 | 15.1 | 13.3 | 15 | 15 | 15 |
| sum | 8.3 | 6.5 | 4.7 | 11.8 | 13.7 | 11.9 | 14.4 | 15.2 | 12.8 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-1051 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAPK3
Peptide Sequence
IADPEHDHTGFLTEYVATR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
190
Peptide End
208
CPTAC ID
CPTAC-1051
Peptide Molecular Mass
2,171.0283
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N V
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y5 (1+) | 16.4 | 10.4 | 11.5 | 18.3 | 10.9 | 11.9 | 24.6 | 15.1 | 16.5 | 15 | 15 | 15 |
| y4 (1+) | 17.4 | 7.1 | 10.7 | 18.1 | 6.7 | 9.9 | 25.1 | 9.8 | 14.6 | 15 | 15 | 15 |
| y3 (1+) | 12.9 | 7.4 | 7.1 | 12 | 7.9 | 7.1 | 17.6 | 10.8 | 10 | 15 | 15 | 15 |
| sum | 8.7 | 4.3 | 7 | 7.5 | 3.7 | 5.3 | 11.5 | 5.7 | 8.8 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-1050 Collapse assay details
Data source: Panorama
Official Gene Symbol
MAPK3
Peptide Modified Sequence
IADPEHDHTGFLT[+80.0]EYVATR
Modification Type
Phospho (ST)
Protein - Site of Modification
202
Peptide - Site of Modification
13
Peptide Start
190
Peptide End
208
CPTAC ID
CPTAC-1050
Peptide Molecular Mass
2,250.9947
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N V
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y5 (1+) | 40.4 | 11.3 | 9.1 | 35.3 | 9.5 | 7 | 53.6 | 14.8 | 11.5 | 15 | 15 | 15 |
| y4 (1+) | 34.9 | 8.3 | 10.5 | 33.3 | 10.1 | 10.9 | 48.2 | 13.1 | 15.1 | 15 | 15 | 15 |
| y3 (1+) | 28.3 | 14.8 | 8 | 24.3 | 19 | 12.1 | 37.3 | 24.1 | 14.5 | 15 | 15 | 15 |
| sum | 16.5 | 4.9 | 5.2 | 14.4 | 6 | 7.2 | 21.9 | 7.7 | 8.9 | 15 | 15 | 15 |