MUTHU KUMARAN | American University of Phnom Penh (original) (raw)

Papers by MUTHU KUMARAN

The effect of the Poorna Chandrothaya Chendooram was studied in male albino rats to explore its a... more The effect of the Poorna Chandrothaya Chendooram was studied in male albino rats to explore its activities on liver. Poorna Chandrothaya Chendooram (3 mg/ml/100 gm) was given orally for seven weeks. The activity of Cytochrome P-450 and, were significantly decreased but the activity of glutathione-S-transferase were increased in the PCC administered rats rather than in control rats. Significant decrease in the activities of Cytochrome P-450 and Met hemoglobin and increase in the level of glutathione-S-transferase , indicate the defensive cytoprotective effects of Poorna Chandrothaya Chendooram .

Journal of Pharmaceutical and Biological Sciences, 2019

The present study was aimed to analysis of bioactive constituents of Papaver somniferum (Poppy se... more The present study was aimed to analysis of bioactive constituents of Papaver somniferum (Poppy seed). The ethanol extract of the seeds were subjected to Phytochemical Screening, Gas chromatography-mass spectroscopic (GC-MS) and Fourier transform infrared spectroscopy (FTIR) analysis. GC-MS analysis of the seeds was performed using a Scion 436-GC Bruker model nd Interpretation on mass spectrum GC-MS was conducted using the database of National Institute Standard and Technology (NIST) and IR spectrum was recorded in spectrophotometer (Shimadzu, IR Affinity1, Japan). Phytochemical screening for seeds extracts indicated the presence of various secondary metabolites like Alkaloid, Cardiac Glycosides, Flavonoid, Phytosterols and Terpenoids. GC-MS analysis of compounds with totally, Thirty Nine volatile compounds major chemical compounds were identified, such as 9-Octadecynoic acid(30.72%), 9-Tetradecen-1-ol, acetate, (E)-(24.02%), 9,12-Octadecadienoic acid, methylester, (E,E)-(7.82%), cis-9,10-Epoxyoctadecan-1-ol (7.43%) and Undec-10-ynoic acid(4.36%). FT-IR analysis of peak values with various functional compounds such as alcohols, phenols, carboxylic acids, aldehydes, amides, amino acids, anhydrides, esters, ketones, Unsaturated aliphatics, aromatics, unsaturated heterocycles, amines, Nitro compound, Alkanes, alkenes, sugars,Sulphur, phosphorus, and fluorine compounds. The present results concluded that the phytochemicals was observed in ethanol extract which revealed that the Papaver somniferum (Poppy seed) is potential use in different fields namely medical and pharmaceuticals and greatly valuable in medicinal practice for the treatment of several human aliments.

Pharmacognosy Journal

MATERIALS AND METHODS Chemicals and reagents All of the chemicals and reagents used in this study... more MATERIALS AND METHODS Chemicals and reagents All of the chemicals and reagents used in this study were of analytical grade.

The anti ulcer effects of aqueous, chloroform and ethanol extracts prepared from the Ipomoea aqua... more The anti ulcer effects of aqueous, chloroform and ethanol extracts prepared from the Ipomoea aquatica forsk leaves was evaluated in rats employing the pylorus ligation, stress induced and aspirin induced models. The parameters taken to assess anti ulcer activity were volume of gastric juice, free acidity, total acidity and ulcer index. The results indicated that chloroform extract significantly (p<0.001) decreased the volume of gastric acid secretions, free acidity and total acidity and ulcer index.

An attempt has been made to study the influence of Solanum trilobatum extract on mercury intoxica... more An attempt has been made to study the influence of Solanum trilobatum extract on mercury intoxicated rats. The animals were treated with sublethal dose of mercuric chloride (2mg/kg body wt.) for 30 days. During the mercury treatment, the level of Aspartate transaminase (AST), Alanine transaminase (ALT) and Alkaline phosphatase (ALP) in serum and lipid peroxidation(LPO) in liver tissue significantly increased and Reduced glutathione (GSH), Glutathione peroxidase (GPx), Catalase (CAT) and Superoxide dismutase (SOD) were simultaneously decreased in the respective tissue. This result indicates that the liver tissue was completely damaged. After mercury treatment, solanum trilobatum extract (150mg/kg body wt.) was administrated in mercury intoxicated animals for another 15 days. Solanum trilobatum extract administration has improved the liver function in mercury intoxicated animal as indicated by declined in the increased level of AST, ALT and ALP in serum and LPO content in liver tissue. The decreased level of antioxidant system (GSH, GPx, CAT and SOD) has been promoted. This result suggested that Solanum trilobatum extract has play a vital role at the time of reducing the mercury toxicity in intoxicated animals.

Under most pathological conditions there is generation of reactive oxygen species and other free ... more Under most pathological conditions there is generation of reactive oxygen species and other free radicals. An increase in the antioxidant reserves of the organism can reduce oxidative stress and some of the plant-derived Drug may help to reduce it. Poorna chandrodayam chendooram are extensively used in Indian traditional medicine. In the present study we have examined the in vitro antioxidant activity of Drug. The antioxidant activities of different concentrations of Drugs were determined by Total polyphenolic content, Ascorbic acid, Total flavonoids, DPPH Radical Scavenging activity, Hydroxyl Radical Scavenging Activity and Nitric Oxide Scavenging activity. The effective antioxidant activity of Poorna chandrodayam chendooram has found increased with increasing concentration.

The aqueous crude extract of the leaves of Azima tetracantha was studied for its phytochemical co... more The aqueous crude extract of the leaves of Azima tetracantha was studied for its phytochemical constituents and antidiarrhoeal activity using castor oil-induced diarrhoea and castor oil-induced enteropooling in rats. The phytochemical studies of the aqueous extract revealed the presence of alkaloids, flavonoids, tannins and saponins. The extract showed significant (p < 0.001) protection against castor oil-induced diarrhoea and castor oil-induced enteropooling at (100 mg/kg). The presence of some of the phytochemicals in the root extract may be responsible for the observed effects, and also the basis for its use in traditional medicine as antidiarrhoeal drug.

The effect of the Poorna Chandrothaya Chendooram was studied in male albino rats to explore its a... more The effect of the Poorna Chandrothaya Chendooram was studied in male albino rats to explore its activities on liver. Poorna Chandrothaya Chendooram (3 mg/ml/100 gm) was given orally for seven weeks. The activity of Cytochrome P-450 and, were significantly decreased but the activity of glutathione-S-transferase were increased in the PCC administered rats rather than in control rats. Significant decrease in the activities of Cytochrome P-450 and Met hemoglobin and increase in the level of glutathione-S-transferase , indicate the defensive cytoprotective effects of Poorna Chandrothaya Chendooram .

The anti ulcer effects of aqueous, chloroform and ethanol extracts prepared from the Ipomoea aqua... more The anti ulcer effects of aqueous, chloroform and ethanol extracts prepared from the Ipomoea aquatica forsk leaves was evaluated in rats employing the pylorus ligation, stress induced and aspirin induced models. The parameters taken to assess anti ulcer activity were volume of gastric juice, free acidity, total acidity and ulcer index. The results indicated that chloroform extract significantly (p<0.001) decreased the volume of gastric acid secretions, free acidity and total acidity and ulcer index.

The impact of mercuric chloride on antioxidant status in the brain tissue of rats, Rattus norvegi... more The impact of mercuric chloride on antioxidant status in the brain tissue of rats, Rattus norvegicus, was studied. Mercury poisoining induced oxida-tive stress leading to generation of free radicals and simultaneous alterations in antioxidant mechanism in animals. In the present study, the level of lipid peroxidation (LPO) was increased in the brain tissue of rats at sub-lethal dose of mercuric chloride (2mg/kg body wt.) treatment for 30 days and simultaneously decreased level of glutathione (GSH), glutathione per-oxidase (GPx), catalase (CAT) and superoxide dismutase (SOD) contents were also noticed in the mercury intoxicated brain tissue. During the recovery span an altered level of antioxidant status was restored to near normal level in the brain tissue of mercury intoxicated animal when treated with ethanolic ginger extract (200mg/kg body wt.) For another 15 days. Ginger play a vital role to detoxify mercury toxicant in the mercury intoxicated animals. The results were discussed in detail.

Antioxidants play an important role in inhibiting and scavenging free radicals, thus providing pr... more Antioxidants play an important role in inhibiting and scavenging free radicals, thus providing protection to human against infections and degenerative diseases. Current research is now directed towards natural antioxidants originated from plants due to safe therapeutics. Azima tetracantha Lam is used in Indian traditional medicine for a wide range of various ailments. To understand the mechanism of pharmacological actions, antioxidant properties of the Azima tetracantha Lam leaves extract were tested using standard in vitro models. The ethanolic extract of Azima tetracantha Lam exhibited strong scavenging effect on superoxide, nitric oxide radical and reducing power radical scavenging assay. The free radical scavenging effect of Azima tetracantha Lam extract was comparable with that of the reference antioxidants. The data obtained in the present study suggests that the extract of Azima tetracantha Lam leaves have potent antioxidant activity against free radicals, prevent oxidative damage to major biomolecules and afford significant protection against oxidative damage.

Medicinal plants contribute in human health care system. Most of the plants utilizes by village p... more Medicinal plants contribute in human health care system. Most of the plants utilizes by village people as a folk medicine. Now we are turned in to medicinal plant analysis of active compounds and conservation aspect. In the present study we had select the three important medicinal plants. We have collected three medicinally important medicinal plants such as Coleus amboinicus, Phyla nodiflora and Vitex negundo for antimicrobial studies. The experiment carried out in the selected medicinal plants leaves. The results are discussed with the available literature.

The present study aimed at evaluating the in vitro antimicrobial effect of crude extract of local... more The present study aimed at evaluating the in vitro antimicrobial effect of crude extract of locally available plants, Cassia auriculata L and Morinda tinctoria Roxb on bacteria (Escherichia coli Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa) and fungi (Candida albicans, Candida tropicalis, Aspergillus niger). The agar disc diffusion method was used to determine the inhibitory effect of both the test plants. Both the plants extract showed inhibitory effect on test organisms. The extract of Cassia auriculata L produced wider zones of inhibition against Candida spp. than the crude extract of Morinda tinctoria Roxb. The minimum inhibitory concentration was also evaluated for the extracts

The present study was carried out to evaluate the antidiabetic effect of Psidium guajava leaves e... more The present study was carried out to evaluate the antidiabetic effect of Psidium guajava leaves extract and to study the activities of hexokinase, aldolase and phosphor glucoisomerase, and gluconeogenic enzymes such asglucose-6-phosphatase and fructose-1,6-diphosphatase in liver and kidney of normal and alloxan induced diabetic rats. Oral administration of ethanolic extract of leaves (250 and 500mg/kg body weight) for 30 days, resulted in significant decrease of blood glucose from 302.67 ± 22.35 to 82.50 ± 04.72 and in a decrease in the activities of glucose-6-phosphatase, fructose-1,6-isphosphatase, aldolase and an increase in the activity of phosphor glucoisomerase and hexokinase in tissues. However, in the case of 250 mg / kg body weight of extract, less activity was observed. The study clearly shows that the leaves extract of Psidium guajava possesses potent antidiabetic activity.

The aim of the study is to analyse the antioxidant effect of oral administration of aqueous extra... more The aim of the study is to analyse the antioxidant effect of oral administration of aqueous extract of Morinda tinctoria (MTR) fresh fruits on blood glucose, haemoglobin, glycosylated haemoglobin, plasma insulin, antioxidant enzymes and lipid peroxidation in liver and kidney to streptozotocin (STZ)-induced diabetic rats. Aqueous extract of Morinda tinctoria (MTR) on blood glucose, haemoglobin, glycosylated haemoglobin, plasma insulin, serum lipid and the levels of lipid peroxides and antioxidant enzymes, such as catalase, superoxide dismutase, glutathione peroxidase and reduced glutathione, were examined in the liver and kidney tissues of control and experimental groups. Oral administration of Morinda tinctoria (MTR) aqueous extract to diabetic rats for 30 days significantly reduced the levels of blood glucose, lipids and lipid peroxidation, but increased the activities of plasma insulin and antioxidant enzymes, like catalase, superoxide dismutase, reduced glutathione and glutathione peroxidase. The Morinda tinctoria (MTR) aqueous extract supplementation is useful in controlling the blood glucose level, improves the plasma insulin, lipid metabolism and is beneficial in preventing diabetic complications from lipid peroxidation and antioxidant systems in experimental diabetic rats; therefore, it could be useful for prevention or early treatment of diabetes mellitus

The different extracts of the Dodonaea viscosa (L). (Family-Sapindaceae) were tested for anti-dia... more The different extracts of the Dodonaea viscosa (L). (Family-Sapindaceae) were tested for anti-diabetic activity, by glucose tolerance test in normal rats and alloxan induced diabetic rats. Aqueous ethanol and butanol extracts had shown significant protection and lowered the blood glucose levels to normal in glucose tolerance test. In alloxan induced diabetic rats the maximum reduction in blood glucose was observed after 3h at a dose level of 250 mg/kg of body weight. The percentage protections by aqueous ethanol and butanol extracts were 30 and 48% respectively. In long term treatment of alloxan induced diabetic rats, the degree of protection was determined by measuring blood glucose, triglycerides, cholesterol and urea levels on 0,3,5,7 and 10th day. Both the extracts showed a significant anti-diabetic activity comparable with that of glibenclamide.. These results indicate that the Dodonaea viscosa (L).extracts possess significant anti – diabetic activity.

The methanol extract of leaves of Pithecellobium dulce Benth. was screened for the analgesic(usin... more The methanol extract of leaves of Pithecellobium dulce Benth. was screened for the analgesic(using hot plate test and acetic acid-induced writhing test in mice) and anti-inflammatory(using rat paw edema test) activity at the doses of 200 and 400mg/kg body weight. A significant (p<0.0005) analgesic effect was observed with200 mg/kg and 400 mg/kg in both tests. The maximum anti-inflammatory response was produced at 3 hour with extract doses of 200 and 400 mg/kg. These results suggest that the methanol extract of Pithecellobium dulce a has exhibited significant analgesic and anti-inflammatory effects, which were comparable with standard drugs

The effect of the Poorna Chandrothaya Chendooram was studied in male albino rats to explore its a... more The effect of the Poorna Chandrothaya Chendooram was studied in male albino rats to explore its activities on liver. Poorna Chandrothaya Chendooram (3 mg/ml/100 gm) was given orally for seven weeks. The activity of Cytochrome P-450 and, were significantly decreased but the activity of glutathione-S-transferase were increased in the PCC administered rats rather than in control rats. Significant decrease in the activities of Cytochrome P-450 and Met hemoglobin and increase in the level of glutathione-S-transferase , indicate the defensive cytoprotective effects of Poorna Chandrothaya Chendooram .

Journal of Pharmaceutical and Biological Sciences, 2019

The present study was aimed to analysis of bioactive constituents of Papaver somniferum (Poppy se... more The present study was aimed to analysis of bioactive constituents of Papaver somniferum (Poppy seed). The ethanol extract of the seeds were subjected to Phytochemical Screening, Gas chromatography-mass spectroscopic (GC-MS) and Fourier transform infrared spectroscopy (FTIR) analysis. GC-MS analysis of the seeds was performed using a Scion 436-GC Bruker model nd Interpretation on mass spectrum GC-MS was conducted using the database of National Institute Standard and Technology (NIST) and IR spectrum was recorded in spectrophotometer (Shimadzu, IR Affinity1, Japan). Phytochemical screening for seeds extracts indicated the presence of various secondary metabolites like Alkaloid, Cardiac Glycosides, Flavonoid, Phytosterols and Terpenoids. GC-MS analysis of compounds with totally, Thirty Nine volatile compounds major chemical compounds were identified, such as 9-Octadecynoic acid(30.72%), 9-Tetradecen-1-ol, acetate, (E)-(24.02%), 9,12-Octadecadienoic acid, methylester, (E,E)-(7.82%), cis-9,10-Epoxyoctadecan-1-ol (7.43%) and Undec-10-ynoic acid(4.36%). FT-IR analysis of peak values with various functional compounds such as alcohols, phenols, carboxylic acids, aldehydes, amides, amino acids, anhydrides, esters, ketones, Unsaturated aliphatics, aromatics, unsaturated heterocycles, amines, Nitro compound, Alkanes, alkenes, sugars,Sulphur, phosphorus, and fluorine compounds. The present results concluded that the phytochemicals was observed in ethanol extract which revealed that the Papaver somniferum (Poppy seed) is potential use in different fields namely medical and pharmaceuticals and greatly valuable in medicinal practice for the treatment of several human aliments.

Pharmacognosy Journal

MATERIALS AND METHODS Chemicals and reagents All of the chemicals and reagents used in this study... more MATERIALS AND METHODS Chemicals and reagents All of the chemicals and reagents used in this study were of analytical grade.

The anti ulcer effects of aqueous, chloroform and ethanol extracts prepared from the Ipomoea aqua... more The anti ulcer effects of aqueous, chloroform and ethanol extracts prepared from the Ipomoea aquatica forsk leaves was evaluated in rats employing the pylorus ligation, stress induced and aspirin induced models. The parameters taken to assess anti ulcer activity were volume of gastric juice, free acidity, total acidity and ulcer index. The results indicated that chloroform extract significantly (p<0.001) decreased the volume of gastric acid secretions, free acidity and total acidity and ulcer index.

An attempt has been made to study the influence of Solanum trilobatum extract on mercury intoxica... more An attempt has been made to study the influence of Solanum trilobatum extract on mercury intoxicated rats. The animals were treated with sublethal dose of mercuric chloride (2mg/kg body wt.) for 30 days. During the mercury treatment, the level of Aspartate transaminase (AST), Alanine transaminase (ALT) and Alkaline phosphatase (ALP) in serum and lipid peroxidation(LPO) in liver tissue significantly increased and Reduced glutathione (GSH), Glutathione peroxidase (GPx), Catalase (CAT) and Superoxide dismutase (SOD) were simultaneously decreased in the respective tissue. This result indicates that the liver tissue was completely damaged. After mercury treatment, solanum trilobatum extract (150mg/kg body wt.) was administrated in mercury intoxicated animals for another 15 days. Solanum trilobatum extract administration has improved the liver function in mercury intoxicated animal as indicated by declined in the increased level of AST, ALT and ALP in serum and LPO content in liver tissue. The decreased level of antioxidant system (GSH, GPx, CAT and SOD) has been promoted. This result suggested that Solanum trilobatum extract has play a vital role at the time of reducing the mercury toxicity in intoxicated animals.

Under most pathological conditions there is generation of reactive oxygen species and other free ... more Under most pathological conditions there is generation of reactive oxygen species and other free radicals. An increase in the antioxidant reserves of the organism can reduce oxidative stress and some of the plant-derived Drug may help to reduce it. Poorna chandrodayam chendooram are extensively used in Indian traditional medicine. In the present study we have examined the in vitro antioxidant activity of Drug. The antioxidant activities of different concentrations of Drugs were determined by Total polyphenolic content, Ascorbic acid, Total flavonoids, DPPH Radical Scavenging activity, Hydroxyl Radical Scavenging Activity and Nitric Oxide Scavenging activity. The effective antioxidant activity of Poorna chandrodayam chendooram has found increased with increasing concentration.

The aqueous crude extract of the leaves of Azima tetracantha was studied for its phytochemical co... more The aqueous crude extract of the leaves of Azima tetracantha was studied for its phytochemical constituents and antidiarrhoeal activity using castor oil-induced diarrhoea and castor oil-induced enteropooling in rats. The phytochemical studies of the aqueous extract revealed the presence of alkaloids, flavonoids, tannins and saponins. The extract showed significant (p < 0.001) protection against castor oil-induced diarrhoea and castor oil-induced enteropooling at (100 mg/kg). The presence of some of the phytochemicals in the root extract may be responsible for the observed effects, and also the basis for its use in traditional medicine as antidiarrhoeal drug.

The effect of the Poorna Chandrothaya Chendooram was studied in male albino rats to explore its a... more The effect of the Poorna Chandrothaya Chendooram was studied in male albino rats to explore its activities on liver. Poorna Chandrothaya Chendooram (3 mg/ml/100 gm) was given orally for seven weeks. The activity of Cytochrome P-450 and, were significantly decreased but the activity of glutathione-S-transferase were increased in the PCC administered rats rather than in control rats. Significant decrease in the activities of Cytochrome P-450 and Met hemoglobin and increase in the level of glutathione-S-transferase , indicate the defensive cytoprotective effects of Poorna Chandrothaya Chendooram .

The anti ulcer effects of aqueous, chloroform and ethanol extracts prepared from the Ipomoea aqua... more The anti ulcer effects of aqueous, chloroform and ethanol extracts prepared from the Ipomoea aquatica forsk leaves was evaluated in rats employing the pylorus ligation, stress induced and aspirin induced models. The parameters taken to assess anti ulcer activity were volume of gastric juice, free acidity, total acidity and ulcer index. The results indicated that chloroform extract significantly (p<0.001) decreased the volume of gastric acid secretions, free acidity and total acidity and ulcer index.

The impact of mercuric chloride on antioxidant status in the brain tissue of rats, Rattus norvegi... more The impact of mercuric chloride on antioxidant status in the brain tissue of rats, Rattus norvegicus, was studied. Mercury poisoining induced oxida-tive stress leading to generation of free radicals and simultaneous alterations in antioxidant mechanism in animals. In the present study, the level of lipid peroxidation (LPO) was increased in the brain tissue of rats at sub-lethal dose of mercuric chloride (2mg/kg body wt.) treatment for 30 days and simultaneously decreased level of glutathione (GSH), glutathione per-oxidase (GPx), catalase (CAT) and superoxide dismutase (SOD) contents were also noticed in the mercury intoxicated brain tissue. During the recovery span an altered level of antioxidant status was restored to near normal level in the brain tissue of mercury intoxicated animal when treated with ethanolic ginger extract (200mg/kg body wt.) For another 15 days. Ginger play a vital role to detoxify mercury toxicant in the mercury intoxicated animals. The results were discussed in detail.

Antioxidants play an important role in inhibiting and scavenging free radicals, thus providing pr... more Antioxidants play an important role in inhibiting and scavenging free radicals, thus providing protection to human against infections and degenerative diseases. Current research is now directed towards natural antioxidants originated from plants due to safe therapeutics. Azima tetracantha Lam is used in Indian traditional medicine for a wide range of various ailments. To understand the mechanism of pharmacological actions, antioxidant properties of the Azima tetracantha Lam leaves extract were tested using standard in vitro models. The ethanolic extract of Azima tetracantha Lam exhibited strong scavenging effect on superoxide, nitric oxide radical and reducing power radical scavenging assay. The free radical scavenging effect of Azima tetracantha Lam extract was comparable with that of the reference antioxidants. The data obtained in the present study suggests that the extract of Azima tetracantha Lam leaves have potent antioxidant activity against free radicals, prevent oxidative damage to major biomolecules and afford significant protection against oxidative damage.

Medicinal plants contribute in human health care system. Most of the plants utilizes by village p... more Medicinal plants contribute in human health care system. Most of the plants utilizes by village people as a folk medicine. Now we are turned in to medicinal plant analysis of active compounds and conservation aspect. In the present study we had select the three important medicinal plants. We have collected three medicinally important medicinal plants such as Coleus amboinicus, Phyla nodiflora and Vitex negundo for antimicrobial studies. The experiment carried out in the selected medicinal plants leaves. The results are discussed with the available literature.

The present study aimed at evaluating the in vitro antimicrobial effect of crude extract of local... more The present study aimed at evaluating the in vitro antimicrobial effect of crude extract of locally available plants, Cassia auriculata L and Morinda tinctoria Roxb on bacteria (Escherichia coli Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa) and fungi (Candida albicans, Candida tropicalis, Aspergillus niger). The agar disc diffusion method was used to determine the inhibitory effect of both the test plants. Both the plants extract showed inhibitory effect on test organisms. The extract of Cassia auriculata L produced wider zones of inhibition against Candida spp. than the crude extract of Morinda tinctoria Roxb. The minimum inhibitory concentration was also evaluated for the extracts

The present study was carried out to evaluate the antidiabetic effect of Psidium guajava leaves e... more The present study was carried out to evaluate the antidiabetic effect of Psidium guajava leaves extract and to study the activities of hexokinase, aldolase and phosphor glucoisomerase, and gluconeogenic enzymes such asglucose-6-phosphatase and fructose-1,6-diphosphatase in liver and kidney of normal and alloxan induced diabetic rats. Oral administration of ethanolic extract of leaves (250 and 500mg/kg body weight) for 30 days, resulted in significant decrease of blood glucose from 302.67 ± 22.35 to 82.50 ± 04.72 and in a decrease in the activities of glucose-6-phosphatase, fructose-1,6-isphosphatase, aldolase and an increase in the activity of phosphor glucoisomerase and hexokinase in tissues. However, in the case of 250 mg / kg body weight of extract, less activity was observed. The study clearly shows that the leaves extract of Psidium guajava possesses potent antidiabetic activity.

The aim of the study is to analyse the antioxidant effect of oral administration of aqueous extra... more The aim of the study is to analyse the antioxidant effect of oral administration of aqueous extract of Morinda tinctoria (MTR) fresh fruits on blood glucose, haemoglobin, glycosylated haemoglobin, plasma insulin, antioxidant enzymes and lipid peroxidation in liver and kidney to streptozotocin (STZ)-induced diabetic rats. Aqueous extract of Morinda tinctoria (MTR) on blood glucose, haemoglobin, glycosylated haemoglobin, plasma insulin, serum lipid and the levels of lipid peroxides and antioxidant enzymes, such as catalase, superoxide dismutase, glutathione peroxidase and reduced glutathione, were examined in the liver and kidney tissues of control and experimental groups. Oral administration of Morinda tinctoria (MTR) aqueous extract to diabetic rats for 30 days significantly reduced the levels of blood glucose, lipids and lipid peroxidation, but increased the activities of plasma insulin and antioxidant enzymes, like catalase, superoxide dismutase, reduced glutathione and glutathione peroxidase. The Morinda tinctoria (MTR) aqueous extract supplementation is useful in controlling the blood glucose level, improves the plasma insulin, lipid metabolism and is beneficial in preventing diabetic complications from lipid peroxidation and antioxidant systems in experimental diabetic rats; therefore, it could be useful for prevention or early treatment of diabetes mellitus

The different extracts of the Dodonaea viscosa (L). (Family-Sapindaceae) were tested for anti-dia... more The different extracts of the Dodonaea viscosa (L). (Family-Sapindaceae) were tested for anti-diabetic activity, by glucose tolerance test in normal rats and alloxan induced diabetic rats. Aqueous ethanol and butanol extracts had shown significant protection and lowered the blood glucose levels to normal in glucose tolerance test. In alloxan induced diabetic rats the maximum reduction in blood glucose was observed after 3h at a dose level of 250 mg/kg of body weight. The percentage protections by aqueous ethanol and butanol extracts were 30 and 48% respectively. In long term treatment of alloxan induced diabetic rats, the degree of protection was determined by measuring blood glucose, triglycerides, cholesterol and urea levels on 0,3,5,7 and 10th day. Both the extracts showed a significant anti-diabetic activity comparable with that of glibenclamide.. These results indicate that the Dodonaea viscosa (L).extracts possess significant anti – diabetic activity.

The methanol extract of leaves of Pithecellobium dulce Benth. was screened for the analgesic(usin... more The methanol extract of leaves of Pithecellobium dulce Benth. was screened for the analgesic(using hot plate test and acetic acid-induced writhing test in mice) and anti-inflammatory(using rat paw edema test) activity at the doses of 200 and 400mg/kg body weight. A significant (p<0.0005) analgesic effect was observed with200 mg/kg and 400 mg/kg in both tests. The maximum anti-inflammatory response was produced at 3 hour with extract doses of 200 and 400 mg/kg. These results suggest that the methanol extract of Pithecellobium dulce a has exhibited significant analgesic and anti-inflammatory effects, which were comparable with standard drugs