Vassilis Mougios | Aristotle University of Thessaloniki (original) (raw)

Papers by Vassilis Mougios

Journal of proteome research, Jan 30, 2015

The delineation of exercise biochemistry by utilizing metabolic fingerprinting has become an esta... more The delineation of exercise biochemistry by utilizing metabolic fingerprinting has become an established strategy. We present a combined RP-UPLC-MS and 1H NMR strategy, supplemented by photometric assays, to monitor the response of the human urinary metabolome to short maximal exercise. Seventeen male volunteers performed two identical sprint sessions on separate days, consisting of three 80-meter maximal runs. Using univariate and multivariate analyses, we followed the fluctuation of 37 metabolites at 1, 1.5 and 2 hours post-exercise. 2-Hydroxyisovalerate, 2-hydroxybutyrate, 2-oxoisocaproate, 3-methyl-2-oxovalerate, 3-hydroxyisobutyrate, 2-oxoisovalerate, 3-hydroxybutyrate, 2-hydroxyisobutyrate, alanine, pyruvate, and fumarate increased 1 h post-exercise and then returned toward baseline. Lactate and acetate were higher than baseline at 1 and 1.5 h. Hypoxanthine and inosine remained above baseline throughout the post-exercise period. Urate decreased at 1 h and increased at 1.5 h be...

Journal of applied physiology (Bethesda, Md. : 1985), 2003

Prolonged moderate exercise increases the concentration of nonesterified fatty acids (NEFA) and t... more Prolonged moderate exercise increases the concentration of nonesterified fatty acids (NEFA) and the ratio of unsaturated to saturated (U/S) NEFA in human plasma. The present study examined the duration of these effects and compared them with the effects of coffee ingestion. On separate days and in random order, seven men and six women 1) cycled for 1 h, 2) ingested coffee containing 5 mg caffeine/kg body mass, 3) ingested coffee followed by exercise 1 h later, and 4) did nothing. Blood samples were drawn at 0, 1, 2, 4, 8, 12, and 24 h. Serum was analyzed for lactate, glucose, glycerol, individual NEFA, triacylglycerols, total cholesterol, and HDL cholesterol. Exercise elevated the U/S NEFA and the percentage of oleate, while decreasing the percentages of palmitate and stearate, at the end of exercise but not subsequently. Consumption of coffee triggered a lower lipolytic response with no alterations in U/S or percentages of individual NEFA. These findings may prove useful in discove...

Mougios, Vassilis, Susanne Ring, Anatoli Petridou, and Michalis G. Nikolaidis. Duration of coffee... more Mougios, Vassilis, Susanne Ring, Anatoli Petridou, and Michalis G. Nikolaidis. Duration of coffee-and exercise-induced changes in the fatty acid profile of human serum. longed moderate exercise increases the concentration of nonesterified fatty acids (NEFA) and the ratio of unsaturated to saturated (U/S) NEFA in human plasma. The present study examined the duration of these effects and compared them with the effects of coffee ingestion. On separate days and in random order, seven men and six women 1) cycled for 1 h, 2) ingested coffee containing 5 mg caffeine/kg body mass, 3) ingested coffee followed by exercise 1 h later, and 4) did nothing. Blood samples were drawn at 0, 1, 2, 4, 8, 12, and 24 h. Serum was analyzed for lactate, glucose, glycerol, individual NEFA, triacylglycerols, total cholesterol, and HDL cholesterol. Exercise elevated the U/S NEFA and the percentage of oleate, while decreasing the percentages of palmitate and stearate, at the end of exercise but not subsequently. Consumption of coffee triggered a lower lipolytic response with no alterations in U/S or percentages of individual NEFA. These findings may prove useful in discovering mechanisms mediating the effects of exercise training on the fatty acid profile of human tissues. glycerol; lactate; nonesterified fatty acids; saturated fatty acids; unsaturated fatty acids Address for reprint requests and other correspondence: V. Mougios, TEFAA, Univ. of Thessaloniki,

time RT-PCR revealed no significant differences between the trained and untrained rats in any of ... more time RT-PCR revealed no significant differences between the trained and untrained rats in any of the muscles studied. Enzyme immunoassay showed significantly lower serum levels of IGF-I and testosterone in the trained compared to the untrained animals. These results suggest that chronic exercise in wheels does not affect IGF-I and AR mRNA levels in rat skeletal muscle, while decreasing the circulating levels of two anabolic factors, i.e., IGF-I and testosterone. It is concluded that IGF-I, AR and testosterone seem to play a marginal role during the adaptation process of rat skeletal muscle to long-term wheel running.

Although chronic exercise is generally believed to improve the lipidemic profile, it is not clear... more Although chronic exercise is generally believed to improve the lipidemic profile, it is not clear whether this is due to exercise training or to other determinants such as the usually low body fat of athletes. The aim of the present study was to compare the lipidemic profile of young lean athletes and non-athletes matched for percentage body fat. Fourteen endurance athletes and fourteen sedentary men participated in the study. Participants provided two blood samples at the beginning and end of a 7-d period, during which they recorded physical activity and food intake. Athletes had significantly higher energy expenditure and energy intake but not significantly different macronutrient composition of their diet from non-athletes. No significant differences were found in serum triacylglycerol, total cholesterol, high-density lipoprotein cholesterol and low-density lipoprotein cholesterol concentrations between groups. These data suggest that athletes and non-athletes with similar body fat do not differ in their lipidemic profiles.

Although several studies have analyzed the fatty acid profile of phospholipids (PL) and, to a les... more Although several studies have analyzed the fatty acid profile of phospholipids (PL) and, to a lesser degree, triacylglycerols (TG) in one or more tissues concurrently, a systematic comparison of the fatty acid composition of different tissues and/or lipid classes is lacking. The purpose of the present study was to compare the fatty acid composition of major lipid classes (PL and TG) in the rat serum, soleus muscle, extensor digitorum longus muscle and the heart. Lipids were extracted from these tissues and analyzed by a combination of thin-layer chromatography and gas chromatography. We found many significant differences in various tissues and lipid classes. Serum had the most distinct fatty acid profile in PL but this "uniqueness" was less apparent in TG, where differences among tissues were in general less frequent than in PL. These two skeletal muscles exhibited similar fatty acid composition in both lipid classes despite their different muscle fiber type composition, denoting that fiber type is not a major determinant of the fatty acid composition of rat skeletal muscle. The fatty acid profile of heart PL was the most different from that of the other tissues examined. PL were rich in polyunsaturated fatty acids, whereas TG were rich in monounsaturated fatty acids. Although the reasons for the differences in fatty acid profile among the tissues examined are largely unknown, it is likely that these differences have an impact upon numerous biological functions.

The Journal of Clinical Endocrinology & Metabolism, 2014

Context: Irisin has been proposed to be a myokine mediating the effect of exercise on adipocyte b... more Context: Irisin has been proposed to be a myokine mediating the effect of exercise on adipocyte browning. The physiology of irisin in humans is not completely understood.

International Journal of Sports Medicine, 2004

time RT-PCR revealed no significant differences between the trained and untrained rats in any of ... more time RT-PCR revealed no significant differences between the trained and untrained rats in any of the muscles studied. Enzyme immunoassay showed significantly lower serum levels of IGF-I and testosterone in the trained compared to the untrained animals. These results suggest that chronic exercise in wheels does not affect IGF-I and AR mRNA levels in rat skeletal muscle, while decreasing the circulating levels of two anabolic factors, i.e., IGF-I and testosterone. It is concluded that IGF-I, AR and testosterone seem to play a marginal role during the adaptation process of rat skeletal muscle to long-term wheel running.

The Journal of Clinical Endocrinology & Metabolism, 2014

Context: Irisin is a recently identified exercise-induced myokine. However, the circulating level... more Context: Irisin is a recently identified exercise-induced myokine. However, the circulating levels of irisin in response to different types of exercise in subjects with metabolic syndrome are unknown.

European Journal of Applied Physiology, 2005

The aim of the present study was to examine the effects of 8 weeks of exercise training on the fa... more The aim of the present study was to examine the effects of 8 weeks of exercise training on the fatty acid composition of phospholipids (PL) and triacylglycerols (TG) in rat liver, skeletal muscle (gastrocnemius medialis), and adipose tissue (epididymal and subcutaneous fat). For this purpose, the relevant tissues of 11 trained rats were compared to those of 14 untrained ones. Training caused several significant differences of large effect size in the concentrations and percentages of individual fatty acids in the aforementioned lipid classes. The fatty acid composition of liver PL, in terms of both concentrations and percentages, changed with training. The TG content of muscle and subcutaneous adipose tissue decreased significantly with training. In contrast to the liver, where no significant differences in the fatty acid profile of TG were found, muscle underwent more significant differences in TG than PL, and adipose tissue only in TG. Most differences were in the same direction in muscle and adipose tissue TG, suggesting a common underlying mechanism. Estimated fatty acid elongase activity was significantly higher, whereas D 9 -desaturase activity was significantly lower in muscle and adipose tissue of the trained rats. In conclusion, exercise training modified the fatty acid composition of liver PL, muscle PL and TG, as well as adipose tissue TG. These findings may aid in delineating the effects of exercise on biolog-ical functions such as membrane properties, cell signaling, and gene expression.

International Journal of Sports Medicine, 2006

The combination of dieting and exercise is considered to be the most effective behavioural approa... more The combination of dieting and exercise is considered to be the most effective behavioural approach for weight loss, and the maintenance of exercise may be one of the best predictors of long-term weight maintenance . Endurance exercise seems to be the most useful kind of exercise in a weight management programme, as resistance exercise has not been shown to increase weight loss or attenuate the loss of fat-free mass typically ac-companying weight loss, despite its clear advantages in increasing muscular strength and function .

International Journal of Sports Medicine, 2007

Introduction ! Nutrition plays a crucial role in athletic performance. Swimming is a demanding sp... more Introduction ! Nutrition plays a crucial role in athletic performance. Swimming is a demanding sport, in which nutritional needs can be extraordinary. A balanced intake of macronutrients is essential, as adequate carbohydrate and protein intakes are necessary for maintaining and enhancing glycogen stores and lean body mass, respectively, during training . Sufficient micronutrient intake is also important, as minerals facilitate the development of swimming performance and contribute to the attainment of optimal physiological function . Nevertheless, reports show suboptimal dietary intakes of swimmers . Such findings could be partially attributed to the lack of nutritional education and scientific monitoring. Moreover, swimmers do not always adjust their nutrient needs to the training stress , but information about possible modifications of the dietary habits of swimmers during different training phases is limited. Training of swimmers needs to be accompanied by appropriate evaluation of a variety of param-eters, including hematologic and biochemical ones. Variations in these parameters may influence the performance capacity of swimmers , and such variations do take place during swimming training, although in diverse directions . Some of these parameters, for instance, parameters of the iron status, are influenced not only by training but also by nutrition . Given the dearth of data on the nutritional status of swimmers during extended training periods, the aim of the present study was to monitor the dietary intakes, along with hematologic and biochemical parameters, of swimmers participating in top-level training during a competitive season of eight months. Anthropometric and performance data were also included in order to provide a thorough picture of the responses to a highly demanding training schedule.

The Journal of Clinical Endocrinology & Metabolism, 2014

Context: The myokine irisin may increase energy expenditure and affect metabolism.

The Open Sports Medicine Journal, 2008

Objectives: The aim of the present study was to examine the acute effects of middle-distance comp... more Objectives: The aim of the present study was to examine the acute effects of middle-distance competitive swimming in cold water on selected physiological and biochemical variables.

British Journal of Nutrition, 2008

We thank Drs Burns and Stensel for their interest in our work. We agree that the published articl... more We thank Drs Burns and Stensel for their interest in our work. We agree that the published articles on the delayed effect of resistance exercise (RE) on postprandial lipaemia (PL) provide controversial results. Three studies 1 -3 employed comparable methodologies in terms of exercise protocol and feeding plan of the subjects, that is, two to four sets of eight to eleven exercises at relatively similar intensities (about 10-12 repetitions maximum (RM)) with 1·5 -2·0 min of rest between sets and a standardised meal on the night prior to the fat tolerance test. These studies reported a decrease 1,3 or no change 2 in the postprandial lipaemic response. A fourth study 4 employed a similar RE protocol but focused on maintaining the subjects in a state of energy balance by increasing food intake up to two-fold after RE v. control on the night prior to the fat tolerance test. This study found no significant effect of RE on PL.

Journal of proteome research, Jan 30, 2015

The delineation of exercise biochemistry by utilizing metabolic fingerprinting has become an esta... more The delineation of exercise biochemistry by utilizing metabolic fingerprinting has become an established strategy. We present a combined RP-UPLC-MS and 1H NMR strategy, supplemented by photometric assays, to monitor the response of the human urinary metabolome to short maximal exercise. Seventeen male volunteers performed two identical sprint sessions on separate days, consisting of three 80-meter maximal runs. Using univariate and multivariate analyses, we followed the fluctuation of 37 metabolites at 1, 1.5 and 2 hours post-exercise. 2-Hydroxyisovalerate, 2-hydroxybutyrate, 2-oxoisocaproate, 3-methyl-2-oxovalerate, 3-hydroxyisobutyrate, 2-oxoisovalerate, 3-hydroxybutyrate, 2-hydroxyisobutyrate, alanine, pyruvate, and fumarate increased 1 h post-exercise and then returned toward baseline. Lactate and acetate were higher than baseline at 1 and 1.5 h. Hypoxanthine and inosine remained above baseline throughout the post-exercise period. Urate decreased at 1 h and increased at 1.5 h be...

Journal of applied physiology (Bethesda, Md. : 1985), 2003

Prolonged moderate exercise increases the concentration of nonesterified fatty acids (NEFA) and t... more Prolonged moderate exercise increases the concentration of nonesterified fatty acids (NEFA) and the ratio of unsaturated to saturated (U/S) NEFA in human plasma. The present study examined the duration of these effects and compared them with the effects of coffee ingestion. On separate days and in random order, seven men and six women 1) cycled for 1 h, 2) ingested coffee containing 5 mg caffeine/kg body mass, 3) ingested coffee followed by exercise 1 h later, and 4) did nothing. Blood samples were drawn at 0, 1, 2, 4, 8, 12, and 24 h. Serum was analyzed for lactate, glucose, glycerol, individual NEFA, triacylglycerols, total cholesterol, and HDL cholesterol. Exercise elevated the U/S NEFA and the percentage of oleate, while decreasing the percentages of palmitate and stearate, at the end of exercise but not subsequently. Consumption of coffee triggered a lower lipolytic response with no alterations in U/S or percentages of individual NEFA. These findings may prove useful in discove...

Mougios, Vassilis, Susanne Ring, Anatoli Petridou, and Michalis G. Nikolaidis. Duration of coffee... more Mougios, Vassilis, Susanne Ring, Anatoli Petridou, and Michalis G. Nikolaidis. Duration of coffee-and exercise-induced changes in the fatty acid profile of human serum. longed moderate exercise increases the concentration of nonesterified fatty acids (NEFA) and the ratio of unsaturated to saturated (U/S) NEFA in human plasma. The present study examined the duration of these effects and compared them with the effects of coffee ingestion. On separate days and in random order, seven men and six women 1) cycled for 1 h, 2) ingested coffee containing 5 mg caffeine/kg body mass, 3) ingested coffee followed by exercise 1 h later, and 4) did nothing. Blood samples were drawn at 0, 1, 2, 4, 8, 12, and 24 h. Serum was analyzed for lactate, glucose, glycerol, individual NEFA, triacylglycerols, total cholesterol, and HDL cholesterol. Exercise elevated the U/S NEFA and the percentage of oleate, while decreasing the percentages of palmitate and stearate, at the end of exercise but not subsequently. Consumption of coffee triggered a lower lipolytic response with no alterations in U/S or percentages of individual NEFA. These findings may prove useful in discovering mechanisms mediating the effects of exercise training on the fatty acid profile of human tissues. glycerol; lactate; nonesterified fatty acids; saturated fatty acids; unsaturated fatty acids Address for reprint requests and other correspondence: V. Mougios, TEFAA, Univ. of Thessaloniki,

time RT-PCR revealed no significant differences between the trained and untrained rats in any of ... more time RT-PCR revealed no significant differences between the trained and untrained rats in any of the muscles studied. Enzyme immunoassay showed significantly lower serum levels of IGF-I and testosterone in the trained compared to the untrained animals. These results suggest that chronic exercise in wheels does not affect IGF-I and AR mRNA levels in rat skeletal muscle, while decreasing the circulating levels of two anabolic factors, i.e., IGF-I and testosterone. It is concluded that IGF-I, AR and testosterone seem to play a marginal role during the adaptation process of rat skeletal muscle to long-term wheel running.

Although chronic exercise is generally believed to improve the lipidemic profile, it is not clear... more Although chronic exercise is generally believed to improve the lipidemic profile, it is not clear whether this is due to exercise training or to other determinants such as the usually low body fat of athletes. The aim of the present study was to compare the lipidemic profile of young lean athletes and non-athletes matched for percentage body fat. Fourteen endurance athletes and fourteen sedentary men participated in the study. Participants provided two blood samples at the beginning and end of a 7-d period, during which they recorded physical activity and food intake. Athletes had significantly higher energy expenditure and energy intake but not significantly different macronutrient composition of their diet from non-athletes. No significant differences were found in serum triacylglycerol, total cholesterol, high-density lipoprotein cholesterol and low-density lipoprotein cholesterol concentrations between groups. These data suggest that athletes and non-athletes with similar body fat do not differ in their lipidemic profiles.

Although several studies have analyzed the fatty acid profile of phospholipids (PL) and, to a les... more Although several studies have analyzed the fatty acid profile of phospholipids (PL) and, to a lesser degree, triacylglycerols (TG) in one or more tissues concurrently, a systematic comparison of the fatty acid composition of different tissues and/or lipid classes is lacking. The purpose of the present study was to compare the fatty acid composition of major lipid classes (PL and TG) in the rat serum, soleus muscle, extensor digitorum longus muscle and the heart. Lipids were extracted from these tissues and analyzed by a combination of thin-layer chromatography and gas chromatography. We found many significant differences in various tissues and lipid classes. Serum had the most distinct fatty acid profile in PL but this "uniqueness" was less apparent in TG, where differences among tissues were in general less frequent than in PL. These two skeletal muscles exhibited similar fatty acid composition in both lipid classes despite their different muscle fiber type composition, denoting that fiber type is not a major determinant of the fatty acid composition of rat skeletal muscle. The fatty acid profile of heart PL was the most different from that of the other tissues examined. PL were rich in polyunsaturated fatty acids, whereas TG were rich in monounsaturated fatty acids. Although the reasons for the differences in fatty acid profile among the tissues examined are largely unknown, it is likely that these differences have an impact upon numerous biological functions.

The Journal of Clinical Endocrinology & Metabolism, 2014

Context: Irisin has been proposed to be a myokine mediating the effect of exercise on adipocyte b... more Context: Irisin has been proposed to be a myokine mediating the effect of exercise on adipocyte browning. The physiology of irisin in humans is not completely understood.

International Journal of Sports Medicine, 2004

time RT-PCR revealed no significant differences between the trained and untrained rats in any of ... more time RT-PCR revealed no significant differences between the trained and untrained rats in any of the muscles studied. Enzyme immunoassay showed significantly lower serum levels of IGF-I and testosterone in the trained compared to the untrained animals. These results suggest that chronic exercise in wheels does not affect IGF-I and AR mRNA levels in rat skeletal muscle, while decreasing the circulating levels of two anabolic factors, i.e., IGF-I and testosterone. It is concluded that IGF-I, AR and testosterone seem to play a marginal role during the adaptation process of rat skeletal muscle to long-term wheel running.

The Journal of Clinical Endocrinology & Metabolism, 2014

Context: Irisin is a recently identified exercise-induced myokine. However, the circulating level... more Context: Irisin is a recently identified exercise-induced myokine. However, the circulating levels of irisin in response to different types of exercise in subjects with metabolic syndrome are unknown.

European Journal of Applied Physiology, 2005

The aim of the present study was to examine the effects of 8 weeks of exercise training on the fa... more The aim of the present study was to examine the effects of 8 weeks of exercise training on the fatty acid composition of phospholipids (PL) and triacylglycerols (TG) in rat liver, skeletal muscle (gastrocnemius medialis), and adipose tissue (epididymal and subcutaneous fat). For this purpose, the relevant tissues of 11 trained rats were compared to those of 14 untrained ones. Training caused several significant differences of large effect size in the concentrations and percentages of individual fatty acids in the aforementioned lipid classes. The fatty acid composition of liver PL, in terms of both concentrations and percentages, changed with training. The TG content of muscle and subcutaneous adipose tissue decreased significantly with training. In contrast to the liver, where no significant differences in the fatty acid profile of TG were found, muscle underwent more significant differences in TG than PL, and adipose tissue only in TG. Most differences were in the same direction in muscle and adipose tissue TG, suggesting a common underlying mechanism. Estimated fatty acid elongase activity was significantly higher, whereas D 9 -desaturase activity was significantly lower in muscle and adipose tissue of the trained rats. In conclusion, exercise training modified the fatty acid composition of liver PL, muscle PL and TG, as well as adipose tissue TG. These findings may aid in delineating the effects of exercise on biolog-ical functions such as membrane properties, cell signaling, and gene expression.

International Journal of Sports Medicine, 2006

The combination of dieting and exercise is considered to be the most effective behavioural approa... more The combination of dieting and exercise is considered to be the most effective behavioural approach for weight loss, and the maintenance of exercise may be one of the best predictors of long-term weight maintenance . Endurance exercise seems to be the most useful kind of exercise in a weight management programme, as resistance exercise has not been shown to increase weight loss or attenuate the loss of fat-free mass typically ac-companying weight loss, despite its clear advantages in increasing muscular strength and function .

International Journal of Sports Medicine, 2007

Introduction ! Nutrition plays a crucial role in athletic performance. Swimming is a demanding sp... more Introduction ! Nutrition plays a crucial role in athletic performance. Swimming is a demanding sport, in which nutritional needs can be extraordinary. A balanced intake of macronutrients is essential, as adequate carbohydrate and protein intakes are necessary for maintaining and enhancing glycogen stores and lean body mass, respectively, during training . Sufficient micronutrient intake is also important, as minerals facilitate the development of swimming performance and contribute to the attainment of optimal physiological function . Nevertheless, reports show suboptimal dietary intakes of swimmers . Such findings could be partially attributed to the lack of nutritional education and scientific monitoring. Moreover, swimmers do not always adjust their nutrient needs to the training stress , but information about possible modifications of the dietary habits of swimmers during different training phases is limited. Training of swimmers needs to be accompanied by appropriate evaluation of a variety of param-eters, including hematologic and biochemical ones. Variations in these parameters may influence the performance capacity of swimmers , and such variations do take place during swimming training, although in diverse directions . Some of these parameters, for instance, parameters of the iron status, are influenced not only by training but also by nutrition . Given the dearth of data on the nutritional status of swimmers during extended training periods, the aim of the present study was to monitor the dietary intakes, along with hematologic and biochemical parameters, of swimmers participating in top-level training during a competitive season of eight months. Anthropometric and performance data were also included in order to provide a thorough picture of the responses to a highly demanding training schedule.

The Journal of Clinical Endocrinology & Metabolism, 2014

Context: The myokine irisin may increase energy expenditure and affect metabolism.

The Open Sports Medicine Journal, 2008

Objectives: The aim of the present study was to examine the acute effects of middle-distance comp... more Objectives: The aim of the present study was to examine the acute effects of middle-distance competitive swimming in cold water on selected physiological and biochemical variables.

British Journal of Nutrition, 2008

We thank Drs Burns and Stensel for their interest in our work. We agree that the published articl... more We thank Drs Burns and Stensel for their interest in our work. We agree that the published articles on the delayed effect of resistance exercise (RE) on postprandial lipaemia (PL) provide controversial results. Three studies 1 -3 employed comparable methodologies in terms of exercise protocol and feeding plan of the subjects, that is, two to four sets of eight to eleven exercises at relatively similar intensities (about 10-12 repetitions maximum (RM)) with 1·5 -2·0 min of rest between sets and a standardised meal on the night prior to the fat tolerance test. These studies reported a decrease 1,3 or no change 2 in the postprandial lipaemic response. A fourth study 4 employed a similar RE protocol but focused on maintaining the subjects in a state of energy balance by increasing food intake up to two-fold after RE v. control on the night prior to the fat tolerance test. This study found no significant effect of RE on PL.