Brooke A Jude | Bard College (original) (raw)

Brooke A Jude

Uploads

Papers by Brooke A Jude

Research paper thumbnail of Draft Genome Sequence of a Violacein-Producing Iodobacter sp. from the Hudson Valley Watershed

Genome Announcements, 2018

Iodobacter species are among a number of freshwater Gram-negative violacein-producing bacteria. J... more Iodobacter species are among a number of freshwater Gram-negative violacein-producing bacteria. Janthinobacterium lividum and Chromobacterium violaceum have had their whole genomes sequenced and annotated. This is the first report of a draft whole-genome sequence of a violacein-producing Iodobacter strain that was isolated from the Hudson Valley watershed.

Research paper thumbnail of A colonization factor links Vibrio cholerae environmental survival and human infection

Research paper thumbnail of Subversion of the innate immune system by a retrovirus

Research paper thumbnail of Sequences within the gag gene of mouse mammary tumor virus needed for mammary gland cell transformation

Journal of …, Jan 1, 2006

Research paper thumbnail of Co-selection of mercury and antibiotic resistance in sphagnum core samples dating back 2000 years

Geomicrobiology …, Jan 1, 2009

Research paper thumbnail of Levels of the secreted Vibrio cholerae attachment factor GbpA are modulated by quorum-sensing-induced proteolysis

Journal of …, Jan 1, 2009

Vibrio cholerae is the etiologic agent of cholera in humans. Intestinal colonization occurs in a ... more Vibrio cholerae is the etiologic agent of cholera in humans. Intestinal colonization occurs in a stepwise fashion, initiating with attachment to the small intestinal epithelium. This attachment is followed by expression of the toxin-coregulated pilus, microcolony formation, and cholera toxin (CT) production. We have recently characterized a secreted attachment factor, GlcNAc binding protein A (GbpA), which functions in attachment to environmental chitin sources as well as to intestinal substrates. Studies have been initiated to define the regulatory network involved in GbpA induction. At low cell density, GbpA was detected in the culture supernatant of all wild-type (WT) strains examined. In contrast, at high cell density, GbpA was undetectable in strains that produce HapR, the central regulator of the cell density-dependent quorum-sensing system of V. cholerae. HapR represses the expression of genes encoding regulators involved in V. cholerae virulence and activates the expression of genes encoding the secreted proteases HapA and PrtV. We show here that GbpA is degraded by HapA and PrtV in a time-dependent fashion. Consistent with this, ΔhapA ΔprtV strains attach to chitin beads more efficiently than either the WT or a ΔhapA ΔprtV ΔgbpA strain. These results suggest a model in which GbpA levels fluctuate in concert with the bacterial production of proteases in response to quorum-sensing signals. This could provide a mechanism for GbpA-mediated attachment to, and detachment from, surfaces in response to environmental cues.

Research paper thumbnail of Characterization of a novel murine retrovirus mixture that facilitates hematopoiesis

Journal of …, Jan 1, 2002

Research paper thumbnail of Genetics of Vibrio cholerae colonization and motility

Vibrio cholerae: genomics and molecular …, Jan 1, 2008

Page 76. Genetics of Vibrio cholerae Colonization and Motility Brooke A. Jude and Ronald K. Taylo... more Page 76. Genetics of Vibrio cholerae Colonization and Motility Brooke A. Jude and Ronald K. Taylor 5 Abstract Survival of Vibrio cholerae, either in the aquatic environment or in the human host, is mediated by appropriate expression ...

Research paper thumbnail of Characterization of factors critical for initial stages of Vibrio cholerae intestinal colonization

UMI, ProQuest ® Dissertations & Theses. The world's most comprehensive collectio... more UMI, ProQuest ® Dissertations & Theses. The world's most comprehensive collection of dissertations and theses. Learn more... ProQuest, Characterization of factors critical for initial stages of Vibrio cholerae intestinal colonization. ...

Research paper thumbnail of Role of FlgT in Anchoring the Flagellum of Vibrio cholerae

Journal of …, Jan 1, 2010

Research paper thumbnail of Identification of a multidrug efflux pump in Flavobacterium johnsoniae

Veterinary research, Jan 1, 2009

Research paper thumbnail of Draft Genome Sequence of a Violacein-Producing Iodobacter sp. from the Hudson Valley Watershed

Genome Announcements, 2018

Iodobacter species are among a number of freshwater Gram-negative violacein-producing bacteria. J... more Iodobacter species are among a number of freshwater Gram-negative violacein-producing bacteria. Janthinobacterium lividum and Chromobacterium violaceum have had their whole genomes sequenced and annotated. This is the first report of a draft whole-genome sequence of a violacein-producing Iodobacter strain that was isolated from the Hudson Valley watershed.

Research paper thumbnail of A colonization factor links Vibrio cholerae environmental survival and human infection

Research paper thumbnail of Subversion of the innate immune system by a retrovirus

Research paper thumbnail of Sequences within the gag gene of mouse mammary tumor virus needed for mammary gland cell transformation

Journal of …, Jan 1, 2006

Research paper thumbnail of Co-selection of mercury and antibiotic resistance in sphagnum core samples dating back 2000 years

Geomicrobiology …, Jan 1, 2009

Research paper thumbnail of Levels of the secreted Vibrio cholerae attachment factor GbpA are modulated by quorum-sensing-induced proteolysis

Journal of …, Jan 1, 2009

Vibrio cholerae is the etiologic agent of cholera in humans. Intestinal colonization occurs in a ... more Vibrio cholerae is the etiologic agent of cholera in humans. Intestinal colonization occurs in a stepwise fashion, initiating with attachment to the small intestinal epithelium. This attachment is followed by expression of the toxin-coregulated pilus, microcolony formation, and cholera toxin (CT) production. We have recently characterized a secreted attachment factor, GlcNAc binding protein A (GbpA), which functions in attachment to environmental chitin sources as well as to intestinal substrates. Studies have been initiated to define the regulatory network involved in GbpA induction. At low cell density, GbpA was detected in the culture supernatant of all wild-type (WT) strains examined. In contrast, at high cell density, GbpA was undetectable in strains that produce HapR, the central regulator of the cell density-dependent quorum-sensing system of V. cholerae. HapR represses the expression of genes encoding regulators involved in V. cholerae virulence and activates the expression of genes encoding the secreted proteases HapA and PrtV. We show here that GbpA is degraded by HapA and PrtV in a time-dependent fashion. Consistent with this, ΔhapA ΔprtV strains attach to chitin beads more efficiently than either the WT or a ΔhapA ΔprtV ΔgbpA strain. These results suggest a model in which GbpA levels fluctuate in concert with the bacterial production of proteases in response to quorum-sensing signals. This could provide a mechanism for GbpA-mediated attachment to, and detachment from, surfaces in response to environmental cues.

Research paper thumbnail of Characterization of a novel murine retrovirus mixture that facilitates hematopoiesis

Journal of …, Jan 1, 2002

Research paper thumbnail of Genetics of Vibrio cholerae colonization and motility

Vibrio cholerae: genomics and molecular …, Jan 1, 2008

Page 76. Genetics of Vibrio cholerae Colonization and Motility Brooke A. Jude and Ronald K. Taylo... more Page 76. Genetics of Vibrio cholerae Colonization and Motility Brooke A. Jude and Ronald K. Taylor 5 Abstract Survival of Vibrio cholerae, either in the aquatic environment or in the human host, is mediated by appropriate expression ...

Research paper thumbnail of Characterization of factors critical for initial stages of Vibrio cholerae intestinal colonization

UMI, ProQuest ® Dissertations & Theses. The world's most comprehensive collectio... more UMI, ProQuest ® Dissertations & Theses. The world's most comprehensive collection of dissertations and theses. Learn more... ProQuest, Characterization of factors critical for initial stages of Vibrio cholerae intestinal colonization. ...

Research paper thumbnail of Role of FlgT in Anchoring the Flagellum of Vibrio cholerae

Journal of …, Jan 1, 2010

Research paper thumbnail of Identification of a multidrug efflux pump in Flavobacterium johnsoniae

Veterinary research, Jan 1, 2009

Log In