RBRC05143 (original) (raw)

Strain Data Sheet

update : 2025-01-20

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Strain Information
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BRC No.	RBRC05143
Type	[Targeted Mutation](../animal%5Fkey/list?strainType=Targeted Mutation&%5F%5Flang%5F%5F=en)
Species	Mus musculus
Strain name	B6.129S2-Fad158/7Maim
Former Common name	B6-Fad158 KO#7
H-2 Haplotype
ES Cell line	D3 [129S2/SvPas]
Background strain
Appearance
Strain development	Developed by Graduate School of Pharmaceutical Sciences, Nagoya City University and Genome Information Research Center, Osaka University in 2006. A neomycin selection cassette was inserted into the exon 4 of the Fad158 gene. C57BL/6J background.
Strain description	Fad158 (Lrrc8c) knockout mice. Homozygous mutant mice do not show body weight gain and insulin resistance by a high-fat diet.
Colony maintenance	Heterozygote x Wild-type
References	Factor for adipocyte differentiation 158 gene disruption prevents the body weight gain and insulin resistance induced by a high-fat diet.Hayashi T, Nozaki Y, Nishizuka M, Ikawa M, Osada S, Imagawa MBiol. Pharm. Bull., 34, 1257-1263 (2011). 21804215The novel gene fad158, having a transmembrane domain and leucine-rich repeat, stimulates adipocyte differentiation.Tominaga K, Kondo C, Kagata T, Hishida T, Nishizuka M, Imagawa MJ. Biol. Chem., 279, 34840-34848 (2004). 15184384

Ordering Information
Donor DNA	mouse Pgk1 promoter, E. coli neomycin resistance gene, mouse Pgk1 polyA, mouse Fad158 genomic DNA
Research application	[Diabetes and Obesity Research](../animal%5Fkey/list?%5F%5Flang%5F%5F=en&researchApplication=Diabetes and Obesity Research)covid19_progression
Specific Term and Conditions	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biol. Pharm. Bull., 34, 1257-1263 (2011).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from Dr.Makoto Nishizuka (nishizuka@hirosaki-u.ac.jp) using the Approval Form. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Depositor	Masayoshi Imagawa (Nagoya City University)
Strain Status	Frozen embryosFrozen sperm
Strain Availability	Recovered litters from cryopreserved sperm (2 to 4 months)Cryopreserved sperm (within 1 month)
Additional Info.	Necessary documents for ordering:Approval form (Japanese / English)Order form (Japanese / English)Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)Acceptance of responsibility for living modified organism (Japanese / English)Genotyping protocol -PCR-