Sherein Saeid Abdelgayed | Cairo University (original) (raw)

Papers by Sherein Saeid Abdelgayed

Skin wounds are the most prevalent daily affections intruding fishes in an aquaculture facility. ... more Skin wounds are the most prevalent daily affections intruding fishes in an aquaculture facility. Such skin affections are considered to be the most common portals of entry for disease agents affecting fishes. This persistent phenomenon necessitates a comprehensive search for an efficient healing therapy to combat the ongoing dermal damage and its pathological consequences. In the current study, the core hypothesis has been vigorously tested through the experimental application of hemodialysate (SolcoserylÒ) solution in several exposure methods including bath, intraperi-toneal (I.P.), intramuscular (I.M.), and local infiltration routes. All tested routes were capable of inducing different degrees of healing in Nile tilapia (Oreochromis niloticus). The core hypothesis of the current research has been experimentally accomplished through assessing the resultant healing degrees based on both gross as well as tissue alteration dynamics among total of 5 experimental groups. Each group consisted of 10 fishes/aquarium. The swift tissue healing of the induced wounds in Nile tilapia were completely achieved 4 days post I.M. injection of the SolcoserylÒ solution (10 ll/50 g fish as a single dose) with an excellent healing grade (+++++). However, bath treatment (1 ml/Lwater as a single dose) and local infiltration (10 ll/50 g fish as a single dose) have

Global Journal of Current Research , 2013

The effect of cadmium (Cd) toxicity, its impact on histopathological changes, the median lethal c... more The effect of cadmium (Cd) toxicity, its impact on histopathological changes, the median lethal concentration (LC50-96 h) and the bioremediation effect of Lemna gibba L and dried Spirulina platensis to grass carp, Ctenopharyngodon idella, were investigated through semi-static acute toxicity test developed with cadmium chloride (CdCl2). This study indicated that:1) Cd poisoning caused structural damage in the fish organs ,2) Lemna gibba L weed was effective in removing Cd from water and reducing its bioaccumulation in liver and muscular tissues of fish, 3) Addition of dried Spirulina platensis reduced significantly (P<0.05) the Cd level uptake as compared to fish exposed to Cd alone and 4) Addition of Lemna gibba L weed and dried Spirulina platensis were remediated the toxic effect of Cd and provided protection against the degenerative action of Cd in grass carp, Ctenopharyngodon idella. Spirulina platensis was effective in removing Hg from water and reducing Hg bioaccumulation in liver and muscular tissues of fish, as well as remediated the toxic effect of Hg and provided protection against the degenerative action of Hg in Nile tilapia, Oreochromis Niloticus.

proved to be second on the race (complete healing was achieved 6 days post treatment with very go... more proved to be second on the race (complete healing was achieved 6 days post treatment with very good grade (+++)). This study demonstrates the clinical value of fish models in establishment of new approach for combating prevalent invasive skin affections in aquaculture.

Feline intestinal lymphosarcomas are mostly caused by Feline Leukemia Virus (FeLV). Unfortunately... more Feline intestinal lymphosarcomas are mostly caused by Feline Leukemia Virus (FeLV). Unfortunately, there is no available vaccine for FeLV in Egypt. The diagnosis of feline intestinal lymphosarcomas depends upon abdominal palpation, x-rays examination, ultrasonography, direct ELISA and histopathology of masses excised during laparotomy. The recorded clinical signs in intestinal lymphosarcoma affected cats were variable including vomiting, fever, anorexia, ascites, anemia, dyspnea, constipation and emaciation. The affected lymph nodes were mesenteric, mediastinal and retropharyngeal. The prevalence of intestinal lymphosarcomas in the examined cats was 4.03 % (11 out of 273 cats). The prevalence was higher in queens than toms (2.93 % and 0.73 % respectively). The Siamese cats had higher prevalence than the Sherazy ones (2.56 % and 1.47 % respectively). X-ray films and ultrasonographic images performed on the eleven cats suffered from intestinal lymphosarcomas revealed ascities and abdominal masses. The comparison of ELISA and histopathology (of excised masses) results showed that 9 out of 11 intestinal lymphosarcoma affected cats were infected with FeLV that proved not all cases of intestinal lymphosarcoma were caused by FeLV. The sensitivity, specificity and accuracy of ELISA to diagnose intestinal lymphosarcoma in cats were 81.81 %, 100 % and 92 % respectively. Gross autopsy of the collected lymph nodes, livers, kidneys revealed that gross lymphadenopathy involving one or more nodes, hepatomegaly and kidney enlargement. Microscopically, the examined tissues specimens showed that the normal architecture of the examined lymph nodes, livers, and kidneys has been replaced by a diffuse infiltrate of both lymphocytes and lymphoblasts. The vast majority of the cells are small lymphocyte-type cells with round basophilic nuclei and a sparse rim of cytoplasm. The eleven intestinal lymphosarcoma affected cats exposed to abdominal exploratory surgery (laparotomy) died at one to three months post-surgery. It is concluded that the vaccination of kittens and cats against FeLV in Egypt is very important to prevent the highly fatal intestinal lymphosarcomas.

process of tendon and the polarity of MES could be an important factor to be considered in treati... more process of tendon and the polarity of MES could be an important factor to be considered in treating tendon injuries.

Oocysts of Eimeria species were collected from 8 Native breed chicken flocks aged 7-8 weeks. Thes... more Oocysts of Eimeria species were collected from 8 Native breed chicken flocks aged 7-8 weeks. These chickens were suffering from bloody dropping, loss of weight, low conversion rate and variable mortalities 3-12% in 6-10 days. Eimeria species' oocysts were sporulated and tested for their infectivity and pathogenicity in male commercial chicks aged 14 days old. The infected chicks showed general signs of ruffled feathers, off food, huddling together with loose dropping and/or bloody dropping with total mortality reached to 90%. The post mortem examination showed hemorrhagic foci in the duodenum, hemorrhagic mucosa in mid intestine and bloody caecal core in two caeci. Eimeria species developmental stages in duodenum, intestine and caecum were histopathologically detected at the 6 th day post infection. The obtained sporulated oocysts were identified according to morphological features, and the calculated shape index were 1.14, 1.19, 1.25 and 1.23 suggestive to be E. tenella, E. necatrix, E. acervulina and E. praecox; respectively. Chicks kept individually in a wire cage were inoculated with one sporulated oocyst for obtaining pure isolate from morphologically identified 10 isolates and for detection the site of infection and histopathological features. Egyptian four local isolates in a pure form were obtained. These isolate, including E. tenella, E. acervulina, E. necatrix, and E. praecox. This isolates were passed in the chicks 14 th day old from increasing their number.

An experiment was performed to evaluate the in vitro efficacy of silver nanoparticles (Ag NP) and... more An experiment was performed to evaluate the in vitro efficacy of silver nanoparticles (Ag NP) and Envirolyte-Anolyte (Env-1/1000) against Flavobacterium columnare. F. columnare was isolated from naturally infected Tilapia fish (Oreochromus niloticus). In vitro, F. columnare treated with Ag NP at 0.1 mg/L for 1 h exhibited a 90% reduction in colony-forming units (CFU).While; Env required half an h at 1/1000 to redue 80 % of colony-forming units (CFU). The results suggest that Ag NPs and Env are beneficial for reducing F. columnare load in the water column and possibly on fish. Further research is warranted to investigate the value of Ag NPs and Env as a therapeutic agent for fish with a columnare infection and as a treatment to prevent further spread of columnare in a fish population.

Limiting factors to the tilapia farming industry are numerous and deeply embedded in the environm... more Limiting factors to the tilapia farming industry are numerous and deeply embedded in the environmental paradigm. Infectious diseases are moved to the top list of limiting factors that harshly endangering the fish health with consequent negative impact on the entire population ecology. Antibiotic selection is of major time-wise and economical importance when an episode of bacterial infection has swiftly attacked certain cultured fish population. In the current study an in-vitro microcosm system has been established to assess the efficacy of two major broad spectrum antibiotics against two septicemic / saprophytic fish pathogens affecting Nile tilapia (Oreochromis niloticus). The antibiogram as well as histopathological examination of the tissue sections of the challenged tilapias have indicated that Florfenicol is more effective than Enrofloxacin in combating Clostridium perfringens infection. However, Enrofloxacin has proved a superior efficacy against pseudomonas infections. Both antibiotics have regulated the nitric oxide / nitric oxide intermediates with an ultimate neutralization of the damage associated with oxidative stresses. Further, the two antibiotics were able to modulate the innate humoral immune responses of the challenged fish. Analysis of the water quality measures of the challenged fish aquaria has revealed a relative increase in water pH and ammonia concentrations in Clostridium perfringens as well as Pseudomonas florescence challenged aquaria. As a positive indicator, ammonia and pH concentration were markedly decreased in both Florfenicol and Enrofloxacin treated aquaria. However, it is worthy to mention that ammonia and pH concentrations were sharply decreased in Florfenicol than Enrofloxacin treated fish aquaria. In conclusion, we recommend that antibiotic of choice can be effectively used not only to treat fish but also to modulate their immune response as well as improve the surrounding environmental water parameters.

Tramadol is an atypical opioid with monoamine re-uptake inhibition properties. The aims of the cu... more Tramadol is an atypical opioid with monoamine re-uptake inhibition properties. The aims of the current study were 1) to investigate the effects of acute oral tramadol administration (sub-lethal dose) on the binding of [ 3 H] RX 821002, a selective α2adrenergic receptor ligand, in the rat brain,2) and its pathological effect on liver of rat .The results revealed that; Tramadol (single dose), at 3 hr after dosing, induced a significant decrease in the alpha2-adrenergic receptors in all brain regions studied (hippocampus; cerebral cortex; thalamus). Liver histopathology revealed that: Leucocytic sinusoidal permeation, congested blood vessels in the portal tract, degeneration of some hepatocytes and vacuolation of hepatocytes.

Seventeen apparently healthy mongrel dogs aged from 1-2 years were used in this study. Ultrasonog... more Seventeen apparently healthy mongrel dogs aged from 1-2 years were used in this study. Ultrasonographic, clinicopathological, cytological and histopathological changes associated with the uses of acetaminophen as maximum therapeutic and toxic doses were studied. The dogs were divided into three groups as follow; first group without receiving any treatment as a control group, second and third group was orally received 128 and 200 mg kg-1 B.W as a maximum therapeutic and toxic dose of acetaminophen, respectively. The experiment continued for 10 days, through which collection of samples from all groups of dogs was performed on the 3 and the 10 day post treatment (p.t). Ultrasonographic examination revealed rd th hyperechodensity of liver parenchyma at 3 day in some dogs. The ultrasonographic changes became more rd pronounced in the third group at the 10 day (p.t) in liver parenchyma and renal cortex expressed by th hyperechogenicity and disappearance of echogenic portal vein walls, especially in toxic dose of acetaminophen. The clinicopathological changes revealed the presence of hemolytic anemia. Increases activities of liver enzymes as well as serum bilirubin, blood glucose, blood urea nitrogen (BUN) and serum creatinine concentrations in all experimental dogs were observed. The cytological examination showed the presence of hepatic toxicity, hyperplasia of splenic tissue, vacuolation and degeneration of renal tubular epithelial cells and different degrees of bone marrow (B.M) hypocellularity. All the above mentioned changes were more pronounced in the toxic dosed group than in the maximum therapeutic dosed group in comparison to the control one. These results were correlated with ultrasonographic and histopathological findings.It was concluded that, acetaminophen should be used only in limited amounts for limited periods as its signs of toxicity appeared even with its therapeutic dose.

This study examines different forms of skeletal deformities detected in fish species collected fr... more This study examines different forms of skeletal deformities detected in fish species collected from two Egyptian aquaculture facilities during two harvest seasons in 2008. Various patterns of skeletal deformities were observed in 19 of 959 fish collected. Deformities were diagnosed using a number of techniques including clinical, radiographic, sonographic and histopathological evaluations. Observed deformities included: lordosis and kyphosis in African catfish (Clarius gariepenius); lateral projection of the mandible, parrot-like head, scoliosis, kyphosis, lordosis and fusion of dorsal with anal fins in Nile tilapia (Oreochromis niloticus); and stump body, scoliosis and mandibular joint deformity in common carp (Cyprinus carpio). Relative incidences of deformities in fish from a facility located in the Sharkia province were 5.12%, 2.66% and 2.85% among catfish, Nile tilapia and common carp, respectively. At a second fish farm located in the Kafr Elsheikh province, the incidences of deformities were 1.02%, 1.55% and 0% among catfish, Nile tilapia and common carp, respectively. Some of the deformities were confirmed using both sonographic and histopathological evaluations. The reasons for the observed deformities could not be definitively determined, but possible aetiologies are discussed.

An in-vitro and in-vivo evaluation of the antiviral activity of Artesunate (ART) as a natural ant... more An in-vitro and in-vivo evaluation of the antiviral activity of Artesunate (ART) as a natural antiviral agent against Bovine Herpes Virus-1 (BoHV-1) and compared its activity in-vitro against Ribavirin. In-vitro evaluation was done on the MDBK cell culture system. Artesunate was able to reduce the release of the virus which was obvious in dropping of the titer of the released virus from cell culture system. Regarding Ribavirin, it showed an antiviral activity on the BoHV-1 but with lower response than that of Artesunate. In-vivo evaluation, three groups of rabbits were used as a laboratory animal's model. The clinical signs of the rabbits and histopathological examination were done on lungs collected from rabbits which were treated with Artesunate, showed a noticeable antiviral activity response against BoHV-1, while those infected with BoHV-1 without Artesunate treatment showed pneumonia and bad general health condition. However, molecular detection by the gel-based PCR was able to detect BoHV-1 in the lung tissues of both groups.

This report describes the clinical and histopathological findings of a rare case of cutaneous ade... more This report describes the clinical and histopathological findings of a rare case of cutaneous adenocarcinoma in a 40-year-old desert tortoise. Surgical excision of the neoplasm improved the general health condition and lo comotion of the tortoise although recurrence of the neoplasm had been recorded 1 year post-surgery. ª 2013 Production and hosting by Elsevier B.V. on behalf

Performance of the current study utilized a well elicited CD-1 seventy five (75) albino mice, fif... more Performance of the current study utilized a well elicited CD-1 seventy five (75) albino mice, fifty (50) of which were adult mature females, while the remaining twenty five (25) were adult mature males. The females albino mice were divided into 5 groups, 10 females in each group separated from males for one month, all groups were get the drug doses twice a week for one month before gestation and during pregnancy until delivery. For breeding purposes each 2 females were mated with 1 male from 5 pm. until 9 am. at the following day. Female mice were classified into five groups. Group I; (control group, dams were injected intramuscularly( i.m) with 0.5 ml of sterile normal saline); group II; (dams were injected i.m. with 5 mg/kg body weight of cadmium chloride (Cd Cl 2 ) dissolved in 0.5 ml of sterile normal saline); group III; (were injected i.m. with Cd Cl 2 as in group II, plus i.m. injection of 10 mg/ kg body weight of vitamin C. group IV; (were injected i.m. with Cd Cl 2 as in group II and given 50 mg/kg body weight of Nigella sativa orally) and group V; (were injected i.m. with Cd Cl 2 and vitamin C as in group III and Nigella sativa as same as in group IV). After delivery, heads of pups were decapitated. The heads were fixed in Bouin`s fixative and prepared routinely for paraffin sectioning and staining for histopathological and immunohistochemical investigations. All control group animals were normally developed, as well as the pups of the remaining groups which treated with vitamin C and / or Nigella sativa with cadmium. Immunohistochemical examination of transforming growth factor alpha(TGF-α) revealed that, control pups (group I)expressed a strong immune reaction in the lingual epithelium and lingual muscles. Cadmium treated mice (group II) revealed degenerative changes in the lining epithelium of the tongue and its muscles. The intermuscular connective tissue was oedematous. This group showed negative immune TGF-α reaction. Cadmium and vitamin C treated animals (group III) expressed moderate TGF-α reaction in the tongue epithelium and faint reaction in the underlying connective tissue cells. Animals treated with cadmium and Nigella sativa (group IV) expressed the same immune reaction as in group III. Cadmium, vitamin C and Nigella sativa treated pups (group V) expressed strong immune reaction of TGF-α similar to that of control group. [Mohamed El-Sakhawy and Sherein Saeid. Histopathological and Immunohistochemical Studies on the Tongue of Albino Mice Offspring from Cadmium Toxicated Dams Treated with Vitamin C and / or Nigella Sativa. Life Sci J 2014;11(2):322-326]. (ISSN:1097-8135). http://www.lifesciencesite.com. 44

The effect of cadmium (Cd) toxicity and its impact on histopathology, haematological, and biochem... more The effect of cadmium (Cd) toxicity and its impact on histopathology, haematological, and biochemical changes in Nile tilapia (Oreochromius niloticus) were studied. Fish (35-45 grams) were expo sed to Cd that resulted in significant reduction (p< 0.05) of the erythrocyte count (RBCs), haemoglobin content (Hb) and haematocrit value (Hct). Significant increases in plasma aspartate aminotranseferase (AST) and alanine aminotransferase (ALT) were observed in fish exposed to Cd. The obtained results indicate that Lemna gibba L weed and their extract are effective in removing Cd from water and reducing Cd bioaccumulation in fish. The addition of Lemna gibba L-extract reduced significantly (p<0.05) the Cd level in water and the metal uptake as compared to fish exposed to Cd alone. The Cd concentration in water was 9.620±0.198 mg L -1 and it decreased significantly (p< 0.05). The Cd accumulation in liver and muscles of fish exposed to Cd alone was higher than that of Lemna gibba L-extract treatment. The addition of Lemna gibba L-extract improves the haematological parameters (RBCs, Hb and Hct) and ameliorates the toxic effect of Cd which indicating the capability of Lemna gibba L-extract to chelate Cd from the media. Subsequently, the Cd toxicity was reduced. This study indicates that Cd poisoning cause structural damage in the fish organs. It is also demonstrated that Lemna gibba L-extract, weed or the weed plus the extract provided protection against the degenerative action of Cd and increased the chance of tissue regeneration.

ShereinSaeid shereinpathology@staff.cu. edu.eg 40 male albino rats were utilized in the current s... more ShereinSaeid shereinpathology@staff.cu. edu.eg 40 male albino rats were utilized in the current study. They were divided into two groups, 20 animals each. The first group was considered control group and daily received saline solution by stomach tube for 4 months. The second group was the experimental group and daily received 40mg/kg body weight of aspartame for the same period. Samples from the parotid salivary glands were fixed in 10% buffered neutral formalin and prepared routinely for paraffin sectioning and staining for histopathological and immunohistochemical investigations of proliferating cell nuclear antigen (PCNA).Aspartame treated animals revealedhistopathological degenerative changes in the parotid salivary glands;theacinar cells showed numerous intracytoplasmic vacuoles and cellular destruction. The nuclei of the acinar cells revealed hyperchromatism, pleomorphism, and numerous mitotic figures.edema was recorded inbetween the acini and ducts. The ducts showed stagnant secretion and the blood vessels were congested. The connective tissue septa increased in thickness and infilterated with chronic inflammatory cells. The immunoexpression of PCNA in the nuclei of the acinar cells was intense after 4 months daily administration of aspartame. To our knowledge, there is no paper in the available literature reporting the immunohistochemical expression of PCNA exclusively in parotid salivary glands. Therefore, the aim of our study is to study the expression of PCNA in the parotid salivary glands.

Global Journal of Current Research , 2013