Ph. Baleshwor Sharma | Central Agricultural University (CAU), Imphal (original) (raw)

Uploads

Papers by Ph. Baleshwor Sharma

Research paper thumbnail of Chemical Science Review and Letters Phenolic profiling of fruit extracts: A discriminative peak analysis through diode array detection in HPLC analysis

An RP-HPLC-PDA method for the simultaneous profiling of phenolic compounds using acidified aqueou... more An RP-HPLC-PDA method for the simultaneous profiling of phenolic compounds using acidified aqueous phase (pH 2.0) and acetonitrile as an organic modifier was developed. Chromatograms were simultaneously acquired at 210, 254, 280, 320, 370 and 510 nm coupled with PDA detector. Such method of simultaneous and multiple acquisitions help resolving ambiguous peaks and enhancing analytical sensitivity. A standard mixture constituted by the photometric sensitive concentration of each of the 34 compounds resolved in the proposed program within 70 min. The program has been successfully applied to profile phenolic constituents of certain fruit extract viz., Garcinia pedunculata, Garcinia xanthochymus, Docynia indica, Rhus semialata, and Averrhoa carambola. About 10 phenolic compounds could be successfully quantified for each fruit sample. The proposed RP-HPLC-PDA gradient program is a cost effective method for profiling and quantification of phenolic compositional units that could be applied to various types of sample.

Research paper thumbnail of Optimization of an HPLC method for the simultaneous quantification of the major organic acids in different fruit extracts

An RP-HPLC method for the simultaneous quantification of organic acids was developed to profile d... more An RP-HPLC method for the simultaneous quantification of organic acids was developed to profile different fruit extracts. An optimized extraction procedure to recover the polar fraction is presented. Aqueous phase acidified to pH 2.0 enabled optimum resolution of a mixture composed of twelve standard acids. Flow rate of 0.6 ml/min rendering column pressure of 965 psi in our specified column has served a vital parameter for precision and reproducibility. A comparable peak alignment of the mixture was set after evaluating LOD and LOQ of each standard. External calibration method was employed; retention time and spiking technique confirmed analyte identity. Repeatability expressed as percentage standard deviation was less than 1.3. Three to six organic acids could be simultaneously quantified for each fruit. The principal organic acid of Garcinia xanthochymus, Garcinia pedunculata, Rhus semialata, Docynia indica, and Averrhoa carambola was found as succinic, (-)-Hydroxycitric acid, malic, malonic and oxalic acid respectively.

Research paper thumbnail of In vitro determination of antioxidant activity by DPPH method: An approach to unify anticipated report on IC50. Ph. Baleshwor Sharma, P. J. Handique and H. Sunitibala Devi, 2014 . In: Current topics in Redox Biology, GJ Sharma & RN Sharan (Eds.). McGraw Hill Education, 2014 New-Delhi, pp: 234-244.

In vitro determination of antioxidant activity by DPPH method: An approach to unify anticipated report on IC50. Ph. Baleshwor Sharma, P. J. Handique and H. Sunitibala Devi, 2014 . In: Current topics in Redox Biology, GJ Sharma & RN Sharan (Eds.). McGraw Hill Education, 2014 New-Delhi, pp: 234-244.

Use of different methodologies for the in vitro determination of antioxidant activity by the DPPH... more Use of different methodologies for the in vitro determination of antioxidant activity by the DPPH• method markedly deviates in the value of the reference standards. A case study of anticipated variation was investigated in 6 model standards and 2 samples. Antioxidant activity of each analyte was simultaneously investigated at four different initial DPPH• concentration. Significant variation for each antioxidant was found. DPPH assay may be treated as a titrimetric test and hence a non-linear regression model which fits the curve subtended by % I vs. log conc. of sample was used to evaluate the IC50.

Keywords: Antioxidant activity, DPPH method, IC50, data-interpretation, non-linear regression.

Research paper thumbnail of A Historical and Taxonomic overview of Garcinia L. and its reproductive ecology. Ph. Baleshwor Sharma, P. J. Handique and H. Sunitibala Devi, 2013

Folia malaysiana Vol. 14 (1) 2013: 63-76

The present review aims to link and streamline the taxonomical delimitations, synonymies, and eni... more The present review aims to link and streamline the taxonomical delimitations, synonymies, and enigmatic issues of apomixis in Garcinia L. The genus is represented by G. mangostana L., the Queen of tropical fruits, and has recently gained renewed interest due to presence of elite natural products. The phenomenon of apomixis in higher plants is limited however; it seems to play a critical role in the genetic diversity of Garcinia. Axiomatic explanations of apomixis in higher plants have been limited and consequently, the generic and subgeneric delimitation in Clusiaceae is still controversial. Refinement can be made through a coherent and correlative study on reproductive ecology, phylogenetics and phytogeography based on classical taxonomy.

Research paper thumbnail of Antioxidant properties, physico-chemical characteristics and proximate composition of five wild fruits of Manipur, India. Ph. Baleshwor Sharma, P. J. Handique and H. Sunitibala Devi, 2013

J Food Sci Technol; DOI 10.1007/s13197-013-1128-2

Antioxidant properties, physico-chemical characteristics and proximate composition of five wild f... more Antioxidant properties, physico-chemical characteristics and proximate composition of five wild fruits viz., Garcinia pedunculata, Garcinia xanthochymus, Docynia indica, Rhus semialata and Averrhoa carambola grown in Manipur, India were presented in the current study. The order of the antioxidant activity and reducing power of the fruit samples was found as R. semialata > D. indica > G. xanthochymus > A. carambola > G. pedunculata. Good correlation coefficient (R2 > 0.99) was found among the three methods applied to determine antioxidant activity. Total phenolic content was positively correlated (R2 = 0.960) with the antioxidant activity however, total flavonoid content was not positively correlated with the antioxidant activity. Physico-chemical and proximate composition of these fruits is documented for the first time.

Research paper thumbnail of Unambiguous identification of Garcinia xanthochymus (Clusiaceae) in Manipur, India. Ph. Baleshwor Sharma, P. J. Handique and H. Sunitibala Devi, 2012

NeBIO I September 2012 I Vol. 3 I Issue 3 I pp 8-11

Garcinia xanthochymus Hook. f. is correctly identified for the first time in Manipur. The vernacu... more Garcinia xanthochymus Hook. f. is correctly identified for the first time in Manipur. The vernacular/local name (Manipur) of the species Heirangkhoi is wrongly recorded elsewhere as Aphanamixis polystachya (Wall.) Parker that created an ambiguous identification. The species that is unanimously known as Heirangkhoi in Manipur was taken for detail taxonomical analysis and resolved as G. Xanthochymus Hook. f.

Research paper thumbnail of Chemical Science Review and Letters Phenolic profiling of fruit extracts: A discriminative peak analysis through diode array detection in HPLC analysis

An RP-HPLC-PDA method for the simultaneous profiling of phenolic compounds using acidified aqueou... more An RP-HPLC-PDA method for the simultaneous profiling of phenolic compounds using acidified aqueous phase (pH 2.0) and acetonitrile as an organic modifier was developed. Chromatograms were simultaneously acquired at 210, 254, 280, 320, 370 and 510 nm coupled with PDA detector. Such method of simultaneous and multiple acquisitions help resolving ambiguous peaks and enhancing analytical sensitivity. A standard mixture constituted by the photometric sensitive concentration of each of the 34 compounds resolved in the proposed program within 70 min. The program has been successfully applied to profile phenolic constituents of certain fruit extract viz., Garcinia pedunculata, Garcinia xanthochymus, Docynia indica, Rhus semialata, and Averrhoa carambola. About 10 phenolic compounds could be successfully quantified for each fruit sample. The proposed RP-HPLC-PDA gradient program is a cost effective method for profiling and quantification of phenolic compositional units that could be applied to various types of sample.

Research paper thumbnail of Optimization of an HPLC method for the simultaneous quantification of the major organic acids in different fruit extracts

An RP-HPLC method for the simultaneous quantification of organic acids was developed to profile d... more An RP-HPLC method for the simultaneous quantification of organic acids was developed to profile different fruit extracts. An optimized extraction procedure to recover the polar fraction is presented. Aqueous phase acidified to pH 2.0 enabled optimum resolution of a mixture composed of twelve standard acids. Flow rate of 0.6 ml/min rendering column pressure of 965 psi in our specified column has served a vital parameter for precision and reproducibility. A comparable peak alignment of the mixture was set after evaluating LOD and LOQ of each standard. External calibration method was employed; retention time and spiking technique confirmed analyte identity. Repeatability expressed as percentage standard deviation was less than 1.3. Three to six organic acids could be simultaneously quantified for each fruit. The principal organic acid of Garcinia xanthochymus, Garcinia pedunculata, Rhus semialata, Docynia indica, and Averrhoa carambola was found as succinic, (-)-Hydroxycitric acid, malic, malonic and oxalic acid respectively.

Research paper thumbnail of In vitro determination of antioxidant activity by DPPH method: An approach to unify anticipated report on IC50. Ph. Baleshwor Sharma, P. J. Handique and H. Sunitibala Devi, 2014 . In: Current topics in Redox Biology, GJ Sharma & RN Sharan (Eds.). McGraw Hill Education, 2014 New-Delhi, pp: 234-244.

In vitro determination of antioxidant activity by DPPH method: An approach to unify anticipated report on IC50. Ph. Baleshwor Sharma, P. J. Handique and H. Sunitibala Devi, 2014 . In: Current topics in Redox Biology, GJ Sharma & RN Sharan (Eds.). McGraw Hill Education, 2014 New-Delhi, pp: 234-244.

Use of different methodologies for the in vitro determination of antioxidant activity by the DPPH... more Use of different methodologies for the in vitro determination of antioxidant activity by the DPPH• method markedly deviates in the value of the reference standards. A case study of anticipated variation was investigated in 6 model standards and 2 samples. Antioxidant activity of each analyte was simultaneously investigated at four different initial DPPH• concentration. Significant variation for each antioxidant was found. DPPH assay may be treated as a titrimetric test and hence a non-linear regression model which fits the curve subtended by % I vs. log conc. of sample was used to evaluate the IC50.

Keywords: Antioxidant activity, DPPH method, IC50, data-interpretation, non-linear regression.

Research paper thumbnail of A Historical and Taxonomic overview of Garcinia L. and its reproductive ecology. Ph. Baleshwor Sharma, P. J. Handique and H. Sunitibala Devi, 2013

Folia malaysiana Vol. 14 (1) 2013: 63-76

The present review aims to link and streamline the taxonomical delimitations, synonymies, and eni... more The present review aims to link and streamline the taxonomical delimitations, synonymies, and enigmatic issues of apomixis in Garcinia L. The genus is represented by G. mangostana L., the Queen of tropical fruits, and has recently gained renewed interest due to presence of elite natural products. The phenomenon of apomixis in higher plants is limited however; it seems to play a critical role in the genetic diversity of Garcinia. Axiomatic explanations of apomixis in higher plants have been limited and consequently, the generic and subgeneric delimitation in Clusiaceae is still controversial. Refinement can be made through a coherent and correlative study on reproductive ecology, phylogenetics and phytogeography based on classical taxonomy.

Research paper thumbnail of Antioxidant properties, physico-chemical characteristics and proximate composition of five wild fruits of Manipur, India. Ph. Baleshwor Sharma, P. J. Handique and H. Sunitibala Devi, 2013

J Food Sci Technol; DOI 10.1007/s13197-013-1128-2

Antioxidant properties, physico-chemical characteristics and proximate composition of five wild f... more Antioxidant properties, physico-chemical characteristics and proximate composition of five wild fruits viz., Garcinia pedunculata, Garcinia xanthochymus, Docynia indica, Rhus semialata and Averrhoa carambola grown in Manipur, India were presented in the current study. The order of the antioxidant activity and reducing power of the fruit samples was found as R. semialata > D. indica > G. xanthochymus > A. carambola > G. pedunculata. Good correlation coefficient (R2 > 0.99) was found among the three methods applied to determine antioxidant activity. Total phenolic content was positively correlated (R2 = 0.960) with the antioxidant activity however, total flavonoid content was not positively correlated with the antioxidant activity. Physico-chemical and proximate composition of these fruits is documented for the first time.

Research paper thumbnail of Unambiguous identification of Garcinia xanthochymus (Clusiaceae) in Manipur, India. Ph. Baleshwor Sharma, P. J. Handique and H. Sunitibala Devi, 2012

NeBIO I September 2012 I Vol. 3 I Issue 3 I pp 8-11

Garcinia xanthochymus Hook. f. is correctly identified for the first time in Manipur. The vernacu... more Garcinia xanthochymus Hook. f. is correctly identified for the first time in Manipur. The vernacular/local name (Manipur) of the species Heirangkhoi is wrongly recorded elsewhere as Aphanamixis polystachya (Wall.) Parker that created an ambiguous identification. The species that is unanimously known as Heirangkhoi in Manipur was taken for detail taxonomical analysis and resolved as G. Xanthochymus Hook. f.