Laura F Pisani | Centro Cardiologico Monzino (original) (raw)

Papers by Laura F Pisani

Journal of Crohn's and Colitis, Jan 30, 2023

could relief colitis through AHR-PAPSS2-PAPST2-mucin sulfation axis, which may present a potentia... more could relief colitis through AHR-PAPSS2-PAPST2-mucin sulfation axis, which may present a potential approach for precise prevention and treatment of IBD.

Frontiers in Gastroenterology

Inflammatory bowel diseases (IBD) are chronic disabling conditions with a complex and multifactor... more Inflammatory bowel diseases (IBD) are chronic disabling conditions with a complex and multifactorial etiology, which is still not completely understood. In the last 20 years, anti-TNF-α antagonists have revolutionized the treatment of IBD, but many patients still do not respond or experience adverse events. Therefore, new biological therapies and small molecules, targeting several different pathways of gut inflammation, have been developed of which some have already been introduced in clinical practice while many others are currently investigated. Moreover, therapeutic procedures such as leukocytapheresis, fecal microbiota transplant and stem cell transplantation are currently being investigated for treating IBD. Lastly, complementary and alternative medicine has become a field of interest for gastroenterologist to reduce symptom burden in IBD patients. In this comprehensive and updated review, a novel classification of current and developing drugs is provided.

Background: Interleukin (IL)-33 is a member of interleukin (IL)-1 family of cytokines which has b... more Background: Interleukin (IL)-33 is a member of interleukin (IL)-1 family of cytokines which has been linked to the development of inflammatory conditions and cancer in the gastrointestinal tract. This study is designed to investigate whether IL-33 has direct effect on human gastric epithelial cells (GES-1) and on human gastric adenocarcinoma cell line (AGS), assessing its role in regulation of cell proliferation and cell cycle, apoptosis and necrosis. Cell cycle regulation was also determined in ex vivo gastric cancer samples obtained during endoscopy and surgical procedures. Methods: cell lines and tissue samples underwent

The project \u201cSharing molecular techniques for food-borne pathogen detection\u201d (acronym F... more The project \u201cSharing molecular techniques for food-borne pathogen detection\u201d (acronym FoBoS), directly aims to set up a molecular procedure to detect pathogens in milk and foodstuffs. The project is casted inside the mainframe of \u201cInnovation4Welfare\u201d project (INTERREG IVc), whose objectives are improving the quality of life by developing a good practice useful for food-borne pathogen early detection. The project gathers a team in which three University research groups blend with five private company Partners with different missions (from agro-food industries to meal preparation and distribution public companies). Their aims will be to analyze, implement and transfer a DNA-array based protocol to the field of food analysis. In a first step, the protocol will be customized to food-borne pathogen detection and several kinds of food matrices. In a second phase, the procedure will be implemented in an industrial context. The protocols validated at the end of WP2 will be transferred to the agro-food and meal distribution companies, as well as other relevant and interested stakeholders for their implementation on Hazard Analysis and Critical Control Points (HACCP) and other food-chain critical points. The expected result of FoBoS will be a new and innovative good practice, which will improve the effectiveness of regional food safety policies through the implementation of a DNA-array based procedure. Thanks to the application of this new and versatile technology, and the participation in the consortium of several typologies of private and public companies, FoBoS will also encourage new business opportunities focussed into innovative new products and services

Digestive and Liver Disease, Mar 1, 2014

The in vitro effects of two omega-3 polyunsaturated fatty acids (PUFAs), namely eicosapentaenoic ... more The in vitro effects of two omega-3 polyunsaturated fatty acids (PUFAs), namely eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), on some inflammatory functions of goat monocytes including phagocytosis, extracellular respiratory burst, as well as the capability to modulate the expression of some pro-inflammatory cytokines, were investigated. Their apoptosis rate and cytoplasmic accumulation of lipid droplets were also studied. Six clinically healthy multiparous lactating goats (Saanen) between 5 and 6 years of age and between 30 and 33 weeks after calving were used for blood collection. Both EPA and DHA upregulated the phagocytosis of monocytes. Results on cytokine regulatory activity were inconsistent. Both EPA and DHA treatment induced a different accumulation of lipid droplets in goat monocytes. Profound effects of EPA and DHA on cellular lipid accumulation were observed. This study demonstrated that both EPA and DHA had important and specific roles in modulating monocyte immune functions and therefore support their use in animal diets not only to increase the nutritional value of milk for human consumption, but also because they could improve animal health

World Journal of Gastroenterology, 2020

Author contributions: Pisani LF performed the majority of the writing; Moriggi M prepared the fig... more Author contributions: Pisani LF performed the majority of the writing; Moriggi M prepared the figure and wrote the technical proteomic paragraphs; Vecchi M provided the input in writing the review; Gelfi C revised the review and gave her support as proteomics expert; and Pastorelli L revised the review and gave his support as clinical expert.

... e nel campo dell'analisi degli alimenti. FoBoS prevede la messa a punto del protocollo p... more ... e nel campo dell'analisi degli alimenti. FoBoS prevede la messa a punto del protocollo per l'identificazione dei patogeni negli alimenti e in alcune matrici alimentari come latte e carni. Tali procedure ottenute al termine del WP2 ...

Journal of International Medical Research, Mar 1, 2022

Objective To investigate the potential inflammatory pathways involved in the development of micro... more Objective To investigate the potential inflammatory pathways involved in the development of microscopic colitis (MC). Methods This prospective study analysed human intestinal tissue that was collected and classified as healthy controls (HC), microscopic colitis (MC) and ulcerative colitis (UC). An RT2 Profiler PCR Array for human inflammatory response and autoimmunity was used to evaluate the expression of 84 specific genes related to the inflammatory and autoimmunity pathways. Data were validated by means of real-time polymerase chain reaction on an independent group of MC intestinal tissue samples. Results This study measured the expression of inflammatory genes in HC ( n = 10), in patients with MC ( n = 8) and in patients with active UC ( n = 10). Of the 84 genes included in the array, the expression of the C-C motif chemokine ligand 19, C-C motif chemokine ligand 21, lymphotoxin beta and complement C3 genes that are involved in the non-canonical nuclear transcription factor kappa B (NF-kB) pathway was increased by 2.96, 6.05, 5.96 and 5.93 times in MC compared with HC, respectively. These results were confirmed by real-time polymerase chain reaction. Conclusions The findings suggest that an impairment of the non-canonical NF-kB pathway is involved in the development of MC.

Inflammatory Bowel Diseases, Jan 4, 2021

Background: Inflammatory bowel diseases (IBD) are characterized by an increased thrombosis risk o... more Background: Inflammatory bowel diseases (IBD) are characterized by an increased thrombosis risk of uncertain etiology. Coagulation derangement arising from inflammation may be a triggering factor. We hypothesized that strong inflammation inhibitors (eg, anti-tumor necrosis factor-α drugs) may affect coagulation. Methods: Forty patients with IBD were compared with 57 control patients for coagulation factors and endogenous thrombin potential (ETP), the latter being the most sensitive marker of in vivo pro-and anticoagulation balance. We measured ETP in the presence and absence of thrombomodulin (the physiologic protein C [PC] activator). Coagulation at different timepoints was also assessed for 28 of these patients during infliximab treatment. Results: The median ETP (nM thrombin × minutes) and range (minimum-maximum) were each higher in patients at baseline than in control patients in both the absence (

Frontiers in Medicine, Jan 31, 2020

Journal of Crohn's and Colitis, 2022

Background: Whether the disease activity of ulcerative colitis (UC) and Crohn's disease (CD) is c... more Background: Whether the disease activity of ulcerative colitis (UC) and Crohn's disease (CD) is correlated with the severity of coronavirus disease 2019 (COVID-19) remains poorly investigated with only few

Therapeutic Advances in Gastroenterology, 2017

The Multimatrix® (MMX®) preparation MMX® is a recently obtained drug formulation developed to fac... more The Multimatrix® (MMX®) preparation MMX® is a recently obtained drug formulation developed to facilitate release of high concentrations of active drugs into the colon, with a homogeneous distribution along all colonic segments, particularly the most distal ones; the distal colonic tracts, indeed, are the most difficult to reach in significant amounts when a drug is given orally. The MMX® formulation is characterized by a lipophilic matrix dispersed in a hydrophilic structure. Indeed, in the last few years, MMX® technology has been widely used in the development of various drugs for the treatment of inflammatory and infectious gastrointestinal diseases localized in the colon. In particular, MMX® mesalamine, budesonide and parnaparin formulations have been investigated in patients with ulcerative colitis, and the first two have reached worldwide registration for the treatment of this disease. Moreover, MMX®-rifamycin is being positively tested in the treatment of colonic bacterial inf...

Frontiers in Medicine, 2017

Journal of Crohn's and Colitis, Feb 1, 2014

S101 to AZA alone. In Huh7 cells, the IC 50 was 80 mM higher after 72 hours incubation with AZA +... more S101 to AZA alone. In Huh7 cells, the IC 50 was 80 mM higher after 72 hours incubation with AZA + 5-ASA compared to AZA alone. Concentration based, TG was the most toxic thiopurine with an IC 50 value of 18 mM after 72 hours incubation in HepaRG cells, compared to AZA (266 mM) and 6-MP (412 mM). Conclusions: Addition of a fixed, non toxic dose of 5-ASA to 6-MP resulted in a decreased in vitro toxicity in hepatoma cells.