Prof. Dr. S. M. Abe Kawsar | University of Chittagong (original) (raw)

Papers by Prof. Dr. S. M. Abe Kawsar

Ovidius University Annals of Chemistry, 2021

Nucleosides and their analogues are an important, well-established class of clinically useful med... more Nucleosides and their analogues are an important, well-established class of clinically useful medicinal agents that exhibit antiviral and anticancer activity. Thus, our research group has focused on the synthesis of new nucleoside derivatives that could be tested for their broad-spectrum biological activity. In this study, two new series of nucleoside derivatives were synthesized from uridine (1) through facile two-step reactions using the direct acylation method, affording 5’-O-acyl uridine derivatives in good yields. The isolated uridine analogs were further transformed into two series of 2’,3’-di-O-acyl derivatives bearing a wide variety of functionalities in a single molecular framework to evaluate their antimicrobial activity. The new synthesized compounds were characterized through physicochemical, elemental and spectroscopic analysis, and all were screened for their in vitro antimicrobial activity against selected human and plant pathogenic strains. The test compounds reveale...

Environmental Science and Pollution Research

Methods in molecular biology, 2020

In the 2010s, a novel lectin family with β-trefoil folding has been identified in marine mussels ... more In the 2010s, a novel lectin family with β-trefoil folding has been identified in marine mussels from the family Mytilidae (phylum Mollusca). "MytiLec-1," the lectin described in this chapter, was the first member of this family to be isolated and characterized from the Mediterranean mussel Mytilus galloprovincialis, a commercially and ecologically important species, spread in marine coastal areas worldwide. MytiLec-1 bound to the sugar moiety of globotriose (Gb3: Galα1-4Galβ1-4Glc), an α-galactoside, leading to apoptosis of Gb3-expressing Burkitt's lymphoma cells. Although the primary structure of MytiLec-1 was quite unusual, its three-dimensional structure was arranged as a β-trefoil fold, which is the typical architecture of "Ricin B chain (or R)-type" lectins, which are found in a broad range of organisms. To date, MytiLec-1-like lectins have been exclusively found in a few species of the mollusk family Mytilidae (M. galloprovincialis, M. trossulus, M. ca...

Hacettepe Journal of Biology and Chemistry, 2015

B u çalışmada, metil 6-O-sinamoil-α-D-glukopiranozid türevlerinin yeni bir serisi, piridinde çeşi... more B u çalışmada, metil 6-O-sinamoil-α-D-glukopiranozid türevlerinin yeni bir serisi, piridinde çeşitli açilleyici ajanlarla, metil α-D-glukopiranozid (1) tepkimesi ile-5 o C'da sentezlendi. Bu amaçla ilk olarak metil α-D-glukopiranosid in seçici sinamalasyonu, doğrudan asilasyon yöntemi ile gerçekleştirildi ve iyi bir verimle metil 6-O-sinamoil-α-D-glukopiranozid elde edildi. İkinci olarak, antimikrobiyal tarama çalışmaları için yeni ürünler elde etmek amacıyla, 6 sinamoil türevleri tek bir molekül çerçevesinde 2,3,4-triO -asil türevlerini içeren geniş fonkisyonel gruplara dönüştürüldü. Yeni sentezlenen bileşiklerin yapıları IR ve NMR ile karakterize edildi. Tüm bileşikler, gıda zehirlenmesi ve disk difüzyon yöntemleri kullanılarak in vitro ortamda antibakteriyel ve antifungal aktiviteleri açısından değerlendirildi. Çalışmada metil 6-O-sinamoil-2,3,4-triO -dekanoilα-D-glukopiranosid bileşiği hem Gram (+) B. subtilise hem de Gram (-) P. aeruginosa mikroorganizmasına karşı yüksek inhibisyon etkisi gösterdi. Test bileşiği, metil 6-O-sinamoil-2,3,4-triO -lauroil-α-D-glukopiranosid A ya karşı maksimum misel büyüme inhibisyonu sergiledi. Açillenmiş türevlerin fungal hattına karşı olan etkisinin bakteriyel patojenlere göre daha yüksek olduğu bulundu. Bu, seçilen patojenlere karşı seçilmiş kimyasalların antimikrobiyal etkisini gösteren ilk raporudur.

Cellolose Chemical Technology, 2021

Several carbohydrate-based drugs are currently being used to treat a number of diseases in humans... more Several carbohydrate-based drugs are currently being used to treat a number of diseases in humans worldwide. Thus, our research group has focused on the synthesis of new methyl α-D-mannopyranoside (MDM) derivatives and their antimicrobial evaluation through computational studies. A series of MDM derivatives (2-6) were synthesized through facile regioselective acylation, using the direct method affording 6-O-(3-chlorobenzoyl) derivatives. This isolated 6-Oderivative was further transformed to 2,3,4-triO -acyl derivatives, bearing a wide variety of functionalities in a single molecular framework. The structures of the newly designed molecules were elucidated with the aid of IR, 1 H NMR, mass spectroscopy, and elemental analysis. The prediction of the activity spectra for the compounds (PASS) and their in vitro antimicrobial evaluation were performed, demonstrating them to be potential antimicrobial agents. The antimicrobial tests demonstrated that the compounds 3 and 5 were the most potent with the minimum inhibitory concentration (MIC) values, ranging from 0.312±0.01 to 1.25±0.03 mg/mL, and minimum bactericidal concentration (MBC) values, ranging from 0.625±0.02 to 2.50±0.05 mg/mL. A quantum chemical study was performed to calculate the thermodynamic, molecular orbital and electrostatic potential properties of the designed compounds. Molecular docking simulation was carried out against SARS-CoV-2 M pro protein 7BQY and 6Y84 to investigate their binding energy and binding tactics with the viral protein, and better binding affinity than that of the parent drug was observed. Also, pharmacokinetic prediction revealed an improved drug-likeness profile for all MDM derivatives.

Trends in Glycoscience and Glycotechnology

Journal of Biological Sciences

Z E T D -glukoz, iyi bir verimle 1,2:5,6-di-O-izopropiliden-α-D-gluko furanoz (1) dönüştürüldü. D... more Z E T D -glukoz, iyi bir verimle 1,2:5,6-di-O-izopropiliden-α-D-gluko furanoz (1) dönüştürüldü. Diaseton glikoz (1)'dan 5,6-O-izopropiliden grubunun çıkarılması, mono asetal glikoz (2)'un elde edilmesini sağlayarak, dikkatli bir şekilde hidroliz ile elde edilmiştir. Burada karşılık gelen 3,5,6-tri-O-asil türevlerinin döşenmiş asile edici bir dizi ajan kullanarak uygun verimle doğrudan 1,2-O-izopropiliden-α-D-gluckofuranoz (2) 'nin seçici asilasyonu rapor edilmektedir. Yeni sentezlenmiş bileşiklerin yapıları FTIR, 1 H-NMR spektroskopisi ve element analizi ile tespit edildi. Sentezlenen tüm bileşikler altı insan patojen bakterisine karşı in vitro antibakteriyel işlevsellik test kimyasalı olarak kullanılmıştır. Değerlendirme çalışması, test kimyasalları iyi antibakteriyel işlevlerine orta derecede tepki verdiğini göstermiştir. Aynı zamanda, test kimyasallarının, Gram-pozitif bakterilere karşı Gram-negatif mikroorganizmalara olduğundan daha etkili olduğu görülmüştür. Anahtar...

Biochemistry (Moscow)

A lectin was purified from Japanese sea hare Aplysia kurodai by lactosyl-agarose affinity chromat... more A lectin was purified from Japanese sea hare Aplysia kurodai by lactosyl-agarose affinity chromatography. The molecular mass of the lectin was determined to be 56 and 32 kDa by SDS-PAGE under non-reducing and reducing conditions, respectively. It was found to agglutinate trypsinized and glutaraldehyde-fixed rabbit and human erythrocytes in the absence of divalent cations. The lectin exhibited stable thermo-tolerance as it retained hemagglutinating activity for 1 h even at 80 degrees C and showed stability at pH 10. By contrast, it was very sensitive at pH less than 5 and in the presence of the sulfhydryl-group preserving reagent, beta-mercaptoethanol. The hemagglutinating activity by the lectin was specifically inhibited by D-galactose, galacturonic acid, methyl-alpha- and methyl-beta-D-galactopyranoside, lactose, melibiose, and asialofetuin. The association rate constant (k(ass)) and dissociation rate constant (k(diss)) were determined for the lectin to be 4.3 x 10(5) M(-1) x sec(-...

Protein and peptide letters, 2009

The glycan-binding profile of a beta-galactoside-binding 15 kDa lectin (Galectin-1) purified from... more The glycan-binding profile of a beta-galactoside-binding 15 kDa lectin (Galectin-1) purified from the oocytes of the American bullfrog, Rana catesbeiana, was studied using 61 pyridyl-aminated oligosaccharides by frontal affinity chromatography. Human blood type-A-hexasaccharide (GalNAcalpha1-3(Fucalpha1-2)Galbeta;1-4GlcNAcbeta1-4Galbeta1-4Glc) was found to exhibit the strongest ligand binding to the galectin while Forssman antigen (GalNAcalpha1-3GalNAcbeta1-3Galalpha1-4Galbeta1-4Glc) and type-A-tetrasaccharide (GalNAcalpha1-3(Fucalpha1-2)Galbeta1-4GlcNAcbeta1-4Glc) were also extensively recognized. The kinetics of affinity of galectin-1 to type-A oligosaccharide was analysed by surface plasmon resonance using neoglycoprotein with type-A oligosaccharides. R. catesbeiana oocyte galectin adhered to human rhabdomyosarcoma cells dose dependently and the activity was specifically cancelled by the neoglycoprotein. It was concluded that galectin-1 from R. catesbeiana oocytes possesses diffe...

RGUHS Journal of Pharmaceutical Sciences, 2012

A 17 kDa MytiLec isolated from the Mediterranean mussel was found to have a novel triple tandem p... more A 17 kDa MytiLec isolated from the Mediterranean mussel was found to have a novel triple tandem primary structure and affinity to an alpha-galactoside, Gb3 (Gala1-4Galb1-4Glc-Cer). The lectin specifically killed Gb3-expressing Burkitt lymphoma Raji cells dose- and glycan-dependently [1]. A crystallographic study showed that MytiLec had beta-trefoil conformation which is a characteristic of Ricin B-chain type (R-type) family lectins, however, the amino acid sequence was not similar. Analogous to Ricin, which killed cultured cells expressing Galb1-4GlcNAc as the ligand, we administered MytiLec to four cultured cell lines that expressed Gb3 glycans and each cell line showed different degrees of dose-sensitivity against MytiLec. FITC-labeled MytiLec was administrated to these cells and its incorporation was observed by confocal laser scanning microscopy. Despite expression of Gb3 on the cell surface, some cell lines incorporated the lectin into the cytosol whereas others did not. Our re...

An a-galactoside-glycosphingolipid, globotriaosyl ceramide (Gb3; Gal a1-4Gal b1-4Glc-Cer) is focu... more An a-galactoside-glycosphingolipid, globotriaosyl ceramide (Gb3; Gal a1-4Gal b1-4Glc-Cer) is focused to be the trigger for cell regulations through the interaction with the glycan-binding receptors. As glycan-binding subunit of Vero toxin that is secreted from pathogenic bacteria, such as O157 acts for the incorporation of the toxin into Gb3 ceramide expressing digestive cells, Gb3-binding lectins isolated from marine invertebrates and fishes were also reported to perform direct cytotoxic activity, up- and down-regulation of cytokines and are the important membrane proteins on cell surface. Two distinct cell regulative activities through Gb3 and lectins that recognize the glycan is discussed in this presentation. MytiLec is an a-galactoside-binding lectin isolated from Mediterranean mussel, Mytilus galloprovincialis with a highly novel primary structure consisted of 149 amino acids and cytotoxic activity against Burkitt lymphoma Raji cells expressing Gb3. On the other hand, another ...

An a-galactoside-glycosphingolipid, globotriaosyl ceramide (Gb3; Gal a1-4Gal b1-4Glc-Cer) is focu... more An a-galactoside-glycosphingolipid, globotriaosyl ceramide (Gb3; Gal a1-4Gal b1-4Glc-Cer) is focused to be the trigger for cell regulations through the interaction with the glycan-binding receptors. As glycan-binding subunit of Vero toxin that is secreted from pathogenic bacteria, such as O157 acts for the incorporation of the toxin into Gb3 ceramide expressing digestive cells, Gb3-binding lectins isolated from marine invertebrates and fishes were also reported to perform direct cytotoxic activity, up- and down-regulation of cytokines and are the important membrane proteins on cell surface. Two distinct cell regulative activites through Gb3 and lectins that recognize the glycan is discussed in this presentation. MytiLec is an a-galactoside-binding lectin isolated from Mediterranean mussel, Mytilus galloprovincialis with a highly novel primary structure consisted of 149 amino acids and cytotoxic activity against Burkitt lymphoma Raji cells expressing Gb3. On the other hand, another G...

Journal of the Bangladesh Chemical Society, 2013

Regioselective benzoylation of methyl 4,6-O-benzylidene-?-D-glucopyranoside (1) using direct acyl... more Regioselective benzoylation of methyl 4,6-O-benzylidene-?-D-glucopyranoside (1) using direct acylation method furnished the methyl 4,6-O-benzylidene-2-O-(4-t-butylbenzoyl)- ?-D-glucopyranoside (2) in an excellent yield. In order to obtain newer products for antimicrobial screening studies, the 2-O-4-t-butylbenzoyl derivative was further transformed to a series of 3-O-acyl derivatives (2-12) containing a wide variety of functionalities in a single molecular framework. Journal of Bangladesh Chemical Society, Vol. 25(2), 101-109, 2012 DOI: http://dx.doi.org/10.3329/jbcs.v25i2.15045

Ovidius University Annals of Chemistry, 2021

Nucleosides and their analogues are an important, well-established class of clinically useful med... more Nucleosides and their analogues are an important, well-established class of clinically useful medicinal agents that exhibit antiviral and anticancer activity. Thus, our research group has focused on the synthesis of new nucleoside derivatives that could be tested for their broad-spectrum biological activity. In this study, two new series of nucleoside derivatives were synthesized from uridine (1) through facile two-step reactions using the direct acylation method, affording 5’-O-acyl uridine derivatives in good yields. The isolated uridine analogs were further transformed into two series of 2’,3’-di-O-acyl derivatives bearing a wide variety of functionalities in a single molecular framework to evaluate their antimicrobial activity. The new synthesized compounds were characterized through physicochemical, elemental and spectroscopic analysis, and all were screened for their in vitro antimicrobial activity against selected human and plant pathogenic strains. The test compounds reveale...

Environmental Science and Pollution Research

Methods in molecular biology, 2020

In the 2010s, a novel lectin family with β-trefoil folding has been identified in marine mussels ... more In the 2010s, a novel lectin family with β-trefoil folding has been identified in marine mussels from the family Mytilidae (phylum Mollusca). "MytiLec-1," the lectin described in this chapter, was the first member of this family to be isolated and characterized from the Mediterranean mussel Mytilus galloprovincialis, a commercially and ecologically important species, spread in marine coastal areas worldwide. MytiLec-1 bound to the sugar moiety of globotriose (Gb3: Galα1-4Galβ1-4Glc), an α-galactoside, leading to apoptosis of Gb3-expressing Burkitt's lymphoma cells. Although the primary structure of MytiLec-1 was quite unusual, its three-dimensional structure was arranged as a β-trefoil fold, which is the typical architecture of "Ricin B chain (or R)-type" lectins, which are found in a broad range of organisms. To date, MytiLec-1-like lectins have been exclusively found in a few species of the mollusk family Mytilidae (M. galloprovincialis, M. trossulus, M. ca...

Hacettepe Journal of Biology and Chemistry, 2015

B u çalışmada, metil 6-O-sinamoil-α-D-glukopiranozid türevlerinin yeni bir serisi, piridinde çeşi... more B u çalışmada, metil 6-O-sinamoil-α-D-glukopiranozid türevlerinin yeni bir serisi, piridinde çeşitli açilleyici ajanlarla, metil α-D-glukopiranozid (1) tepkimesi ile-5 o C'da sentezlendi. Bu amaçla ilk olarak metil α-D-glukopiranosid in seçici sinamalasyonu, doğrudan asilasyon yöntemi ile gerçekleştirildi ve iyi bir verimle metil 6-O-sinamoil-α-D-glukopiranozid elde edildi. İkinci olarak, antimikrobiyal tarama çalışmaları için yeni ürünler elde etmek amacıyla, 6 sinamoil türevleri tek bir molekül çerçevesinde 2,3,4-triO -asil türevlerini içeren geniş fonkisyonel gruplara dönüştürüldü. Yeni sentezlenen bileşiklerin yapıları IR ve NMR ile karakterize edildi. Tüm bileşikler, gıda zehirlenmesi ve disk difüzyon yöntemleri kullanılarak in vitro ortamda antibakteriyel ve antifungal aktiviteleri açısından değerlendirildi. Çalışmada metil 6-O-sinamoil-2,3,4-triO -dekanoilα-D-glukopiranosid bileşiği hem Gram (+) B. subtilise hem de Gram (-) P. aeruginosa mikroorganizmasına karşı yüksek inhibisyon etkisi gösterdi. Test bileşiği, metil 6-O-sinamoil-2,3,4-triO -lauroil-α-D-glukopiranosid A ya karşı maksimum misel büyüme inhibisyonu sergiledi. Açillenmiş türevlerin fungal hattına karşı olan etkisinin bakteriyel patojenlere göre daha yüksek olduğu bulundu. Bu, seçilen patojenlere karşı seçilmiş kimyasalların antimikrobiyal etkisini gösteren ilk raporudur.

Cellolose Chemical Technology, 2021

Several carbohydrate-based drugs are currently being used to treat a number of diseases in humans... more Several carbohydrate-based drugs are currently being used to treat a number of diseases in humans worldwide. Thus, our research group has focused on the synthesis of new methyl α-D-mannopyranoside (MDM) derivatives and their antimicrobial evaluation through computational studies. A series of MDM derivatives (2-6) were synthesized through facile regioselective acylation, using the direct method affording 6-O-(3-chlorobenzoyl) derivatives. This isolated 6-Oderivative was further transformed to 2,3,4-triO -acyl derivatives, bearing a wide variety of functionalities in a single molecular framework. The structures of the newly designed molecules were elucidated with the aid of IR, 1 H NMR, mass spectroscopy, and elemental analysis. The prediction of the activity spectra for the compounds (PASS) and their in vitro antimicrobial evaluation were performed, demonstrating them to be potential antimicrobial agents. The antimicrobial tests demonstrated that the compounds 3 and 5 were the most potent with the minimum inhibitory concentration (MIC) values, ranging from 0.312±0.01 to 1.25±0.03 mg/mL, and minimum bactericidal concentration (MBC) values, ranging from 0.625±0.02 to 2.50±0.05 mg/mL. A quantum chemical study was performed to calculate the thermodynamic, molecular orbital and electrostatic potential properties of the designed compounds. Molecular docking simulation was carried out against SARS-CoV-2 M pro protein 7BQY and 6Y84 to investigate their binding energy and binding tactics with the viral protein, and better binding affinity than that of the parent drug was observed. Also, pharmacokinetic prediction revealed an improved drug-likeness profile for all MDM derivatives.

Trends in Glycoscience and Glycotechnology

Journal of Biological Sciences

Z E T D -glukoz, iyi bir verimle 1,2:5,6-di-O-izopropiliden-α-D-gluko furanoz (1) dönüştürüldü. D... more Z E T D -glukoz, iyi bir verimle 1,2:5,6-di-O-izopropiliden-α-D-gluko furanoz (1) dönüştürüldü. Diaseton glikoz (1)'dan 5,6-O-izopropiliden grubunun çıkarılması, mono asetal glikoz (2)'un elde edilmesini sağlayarak, dikkatli bir şekilde hidroliz ile elde edilmiştir. Burada karşılık gelen 3,5,6-tri-O-asil türevlerinin döşenmiş asile edici bir dizi ajan kullanarak uygun verimle doğrudan 1,2-O-izopropiliden-α-D-gluckofuranoz (2) 'nin seçici asilasyonu rapor edilmektedir. Yeni sentezlenmiş bileşiklerin yapıları FTIR, 1 H-NMR spektroskopisi ve element analizi ile tespit edildi. Sentezlenen tüm bileşikler altı insan patojen bakterisine karşı in vitro antibakteriyel işlevsellik test kimyasalı olarak kullanılmıştır. Değerlendirme çalışması, test kimyasalları iyi antibakteriyel işlevlerine orta derecede tepki verdiğini göstermiştir. Aynı zamanda, test kimyasallarının, Gram-pozitif bakterilere karşı Gram-negatif mikroorganizmalara olduğundan daha etkili olduğu görülmüştür. Anahtar...

Biochemistry (Moscow)

A lectin was purified from Japanese sea hare Aplysia kurodai by lactosyl-agarose affinity chromat... more A lectin was purified from Japanese sea hare Aplysia kurodai by lactosyl-agarose affinity chromatography. The molecular mass of the lectin was determined to be 56 and 32 kDa by SDS-PAGE under non-reducing and reducing conditions, respectively. It was found to agglutinate trypsinized and glutaraldehyde-fixed rabbit and human erythrocytes in the absence of divalent cations. The lectin exhibited stable thermo-tolerance as it retained hemagglutinating activity for 1 h even at 80 degrees C and showed stability at pH 10. By contrast, it was very sensitive at pH less than 5 and in the presence of the sulfhydryl-group preserving reagent, beta-mercaptoethanol. The hemagglutinating activity by the lectin was specifically inhibited by D-galactose, galacturonic acid, methyl-alpha- and methyl-beta-D-galactopyranoside, lactose, melibiose, and asialofetuin. The association rate constant (k(ass)) and dissociation rate constant (k(diss)) were determined for the lectin to be 4.3 x 10(5) M(-1) x sec(-...

Protein and peptide letters, 2009

The glycan-binding profile of a beta-galactoside-binding 15 kDa lectin (Galectin-1) purified from... more The glycan-binding profile of a beta-galactoside-binding 15 kDa lectin (Galectin-1) purified from the oocytes of the American bullfrog, Rana catesbeiana, was studied using 61 pyridyl-aminated oligosaccharides by frontal affinity chromatography. Human blood type-A-hexasaccharide (GalNAcalpha1-3(Fucalpha1-2)Galbeta;1-4GlcNAcbeta1-4Galbeta1-4Glc) was found to exhibit the strongest ligand binding to the galectin while Forssman antigen (GalNAcalpha1-3GalNAcbeta1-3Galalpha1-4Galbeta1-4Glc) and type-A-tetrasaccharide (GalNAcalpha1-3(Fucalpha1-2)Galbeta1-4GlcNAcbeta1-4Glc) were also extensively recognized. The kinetics of affinity of galectin-1 to type-A oligosaccharide was analysed by surface plasmon resonance using neoglycoprotein with type-A oligosaccharides. R. catesbeiana oocyte galectin adhered to human rhabdomyosarcoma cells dose dependently and the activity was specifically cancelled by the neoglycoprotein. It was concluded that galectin-1 from R. catesbeiana oocytes possesses diffe...

RGUHS Journal of Pharmaceutical Sciences, 2012

A 17 kDa MytiLec isolated from the Mediterranean mussel was found to have a novel triple tandem p... more A 17 kDa MytiLec isolated from the Mediterranean mussel was found to have a novel triple tandem primary structure and affinity to an alpha-galactoside, Gb3 (Gala1-4Galb1-4Glc-Cer). The lectin specifically killed Gb3-expressing Burkitt lymphoma Raji cells dose- and glycan-dependently [1]. A crystallographic study showed that MytiLec had beta-trefoil conformation which is a characteristic of Ricin B-chain type (R-type) family lectins, however, the amino acid sequence was not similar. Analogous to Ricin, which killed cultured cells expressing Galb1-4GlcNAc as the ligand, we administered MytiLec to four cultured cell lines that expressed Gb3 glycans and each cell line showed different degrees of dose-sensitivity against MytiLec. FITC-labeled MytiLec was administrated to these cells and its incorporation was observed by confocal laser scanning microscopy. Despite expression of Gb3 on the cell surface, some cell lines incorporated the lectin into the cytosol whereas others did not. Our re...

An a-galactoside-glycosphingolipid, globotriaosyl ceramide (Gb3; Gal a1-4Gal b1-4Glc-Cer) is focu... more An a-galactoside-glycosphingolipid, globotriaosyl ceramide (Gb3; Gal a1-4Gal b1-4Glc-Cer) is focused to be the trigger for cell regulations through the interaction with the glycan-binding receptors. As glycan-binding subunit of Vero toxin that is secreted from pathogenic bacteria, such as O157 acts for the incorporation of the toxin into Gb3 ceramide expressing digestive cells, Gb3-binding lectins isolated from marine invertebrates and fishes were also reported to perform direct cytotoxic activity, up- and down-regulation of cytokines and are the important membrane proteins on cell surface. Two distinct cell regulative activities through Gb3 and lectins that recognize the glycan is discussed in this presentation. MytiLec is an a-galactoside-binding lectin isolated from Mediterranean mussel, Mytilus galloprovincialis with a highly novel primary structure consisted of 149 amino acids and cytotoxic activity against Burkitt lymphoma Raji cells expressing Gb3. On the other hand, another ...

An a-galactoside-glycosphingolipid, globotriaosyl ceramide (Gb3; Gal a1-4Gal b1-4Glc-Cer) is focu... more An a-galactoside-glycosphingolipid, globotriaosyl ceramide (Gb3; Gal a1-4Gal b1-4Glc-Cer) is focused to be the trigger for cell regulations through the interaction with the glycan-binding receptors. As glycan-binding subunit of Vero toxin that is secreted from pathogenic bacteria, such as O157 acts for the incorporation of the toxin into Gb3 ceramide expressing digestive cells, Gb3-binding lectins isolated from marine invertebrates and fishes were also reported to perform direct cytotoxic activity, up- and down-regulation of cytokines and are the important membrane proteins on cell surface. Two distinct cell regulative activites through Gb3 and lectins that recognize the glycan is discussed in this presentation. MytiLec is an a-galactoside-binding lectin isolated from Mediterranean mussel, Mytilus galloprovincialis with a highly novel primary structure consisted of 149 amino acids and cytotoxic activity against Burkitt lymphoma Raji cells expressing Gb3. On the other hand, another G...

Journal of the Bangladesh Chemical Society, 2013

Regioselective benzoylation of methyl 4,6-O-benzylidene-?-D-glucopyranoside (1) using direct acyl... more Regioselective benzoylation of methyl 4,6-O-benzylidene-?-D-glucopyranoside (1) using direct acylation method furnished the methyl 4,6-O-benzylidene-2-O-(4-t-butylbenzoyl)- ?-D-glucopyranoside (2) in an excellent yield. In order to obtain newer products for antimicrobial screening studies, the 2-O-4-t-butylbenzoyl derivative was further transformed to a series of 3-O-acyl derivatives (2-12) containing a wide variety of functionalities in a single molecular framework. Journal of Bangladesh Chemical Society, Vol. 25(2), 101-109, 2012 DOI: http://dx.doi.org/10.3329/jbcs.v25i2.15045