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Papers by monica mamani
Revista Medica De Chile, 2002
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Talanta, 2006
An optimized capillary zone electrophoresis (CZE) method for the analysis of tetracycline (TC), c... more An optimized capillary zone electrophoresis (CZE) method for the analysis of tetracycline (TC), chlortetracycline (CTC), oxytetracycline (OTC) and doxycycline (DXC) is described. Using fused-silica capillaries, the influence of the electrolyte composition, pH and concentration, as well as temperature and applied voltage were investigated. A factorial and central composite design was performed to optimize the method in a simple way. The optimal separation conditions were 50 mmol L−1 sodium carbonate + 1 mmol L−1 EDTA, pH 10; voltage 13 kV and temperature 23 °C. The method was validated for TC determination in pharmaceuticals through the following performance criteria: linearity and linear range, sensitivity, selectivity, intra-assay and inter-assay precision, detectability, accuracy and ruggedness. In comparison with the recommended HPLC method in the United States Pharmacopeia, this CZE-method exhibited the same performance as the official method, with the advantage that the same method could be used for the simultaneous determination of the different tetracyclines in pharmaceutical formulations.
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Journal of Pharmaceutical and Biomedical Analysis, 2005
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Talanta, 2008
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Food Chemistry, 2009
A high-performance liquid chromatographic method for the simultaneous determination of tetracycli... more A high-performance liquid chromatographic method for the simultaneous determination of tetracycline, oxytetracycline, chlortetracycline, sulfamethazine, sulfaquinoxaline, sulfamethoxazole and chloramphenicol in milk has been developed. The determination of these antimicrobials was carried out using HPLC-DAD with a C18 hybrid column and gradient elution with a mobile phase composed of an aqueous phase of 0.075 mol L−1 sodium acetate, 0.035 mol L−1 calcium chloride and 0.025 mol L−1sodium EDTA, pH 7 and an organic phase of methanol:acetonitrile, 75:25 v/v. Sample preparation involved protein precipitation followed by solid-phase extraction using a polymeric cartridge. The method was validated and applied for the analysis of pasteurised milk samples commercialised in Brazil. The limits of quantitation for all antimicrobials, with the exception of choramphenicol, were below the maximum residue limit, which indicates that the method is appropriate for the determination of these antimicrobials in milk.
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Bookmarks Related papers MentionsView impact
Revista Medica De Chile, 2002
Bookmarks Related papers MentionsView impact
Talanta, 2006
An optimized capillary zone electrophoresis (CZE) method for the analysis of tetracycline (TC), c... more An optimized capillary zone electrophoresis (CZE) method for the analysis of tetracycline (TC), chlortetracycline (CTC), oxytetracycline (OTC) and doxycycline (DXC) is described. Using fused-silica capillaries, the influence of the electrolyte composition, pH and concentration, as well as temperature and applied voltage were investigated. A factorial and central composite design was performed to optimize the method in a simple way. The optimal separation conditions were 50 mmol L−1 sodium carbonate + 1 mmol L−1 EDTA, pH 10; voltage 13 kV and temperature 23 °C. The method was validated for TC determination in pharmaceuticals through the following performance criteria: linearity and linear range, sensitivity, selectivity, intra-assay and inter-assay precision, detectability, accuracy and ruggedness. In comparison with the recommended HPLC method in the United States Pharmacopeia, this CZE-method exhibited the same performance as the official method, with the advantage that the same method could be used for the simultaneous determination of the different tetracyclines in pharmaceutical formulations.
Bookmarks Related papers MentionsView impact
Journal of Pharmaceutical and Biomedical Analysis, 2005
Bookmarks Related papers MentionsView impact
Talanta, 2008
Bookmarks Related papers MentionsView impact
Food Chemistry, 2009
A high-performance liquid chromatographic method for the simultaneous determination of tetracycli... more A high-performance liquid chromatographic method for the simultaneous determination of tetracycline, oxytetracycline, chlortetracycline, sulfamethazine, sulfaquinoxaline, sulfamethoxazole and chloramphenicol in milk has been developed. The determination of these antimicrobials was carried out using HPLC-DAD with a C18 hybrid column and gradient elution with a mobile phase composed of an aqueous phase of 0.075 mol L−1 sodium acetate, 0.035 mol L−1 calcium chloride and 0.025 mol L−1sodium EDTA, pH 7 and an organic phase of methanol:acetonitrile, 75:25 v/v. Sample preparation involved protein precipitation followed by solid-phase extraction using a polymeric cartridge. The method was validated and applied for the analysis of pasteurised milk samples commercialised in Brazil. The limits of quantitation for all antimicrobials, with the exception of choramphenicol, were below the maximum residue limit, which indicates that the method is appropriate for the determination of these antimicrobials in milk.
Bookmarks Related papers MentionsView impact
Bookmarks Related papers MentionsView impact