Victor Nunez | Corporacion colombiana de investigación agropecuaria (original) (raw)
Address: Bogotá, Distrito Especial, Colombia
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La determinación del sexo en plantas de papaya es considerado un sistema intrigante, debido a que... more La determinación del sexo en plantas de papaya es considerado un sistema intrigante, debido a que esta especie presenta tres sexos (macho, hembra y hermafrodita) determinados por un locus multialélico. Además, esta especie no presenta cromosomas sexuales morfológicamente diferenciables. Los marcadores moleculares pueden asociarse a características de interés, como en el presente caso, al sexo en plántulas de papaya. Con el objetivo de identificar marcadores moleculares que permitan una rápida identificación del sexo en genotipos colombianos de plántulas de papaya, se aplicó la técnica RAPD (amplificación aleatoria de polimorfismos del ADN). El estudio encontró tres marcadores RAPD polimórficos, los cuales permitieron diferenciar los sexos de la papaya. Dos marcadores fueron específicos para plantas macho y hermafrodita, y un tercero para plantas hembra. Estos nuevos marcadores moleculares podrán ser beneficiosos en la determinación del sexo en genotipos colombianos de papaya.
A B S T R A C T In this study the molecular markers SCAR-SDSP, SCAR T1 and W11 -reported as sex d... more A B S T R A C T In this study the molecular markers SCAR-SDSP, SCAR T1 and W11 -reported as sex discriminators at seedling level-, were used to validate the effectiveness in flowered plants in the field. SCAR-SDSP amplified a band of 369 bp in male and hermaphrodite plants which allows corroborate the results obtained with this marker, developed at Corpoica using Colombian genotypes, in a previous study. With the use of the SCAR T1 and W11 markers combined in one reaction, it was possible to differentiate female and hermaphrodite plants. When the sex prediction was done with a blind sample using two months-old plantlets, from commercial seeds, a ratio of 1:1 female:hermaphrodite was observed, according to the segregation pattern of a cross between these two types of plants. The amplification with the SCAR W11 generated a band of 800 bp in male and hermaphrodite plants, independently of the sample's place of origin. However, when this band was sequenced and analyzed with the BLAST program, the analysis showed 98% identity between male and hermaphrodite sequence.
Sex type determination in papaya (Carica papaya L.) is very important for crop improvement proces... more Sex type determination in papaya (Carica papaya L.) is very important for crop improvement processes because it accelerates the identification of the fruitful plants. The use of molecular technology provides a quick and reliable identification of sex types in plantlets growing in seedbeds. Random amplified polymorphic DNA (RAPD) markers were used to determine the sex types of Colombian cultivars of dioecious papaya genotypes. This species has three sex types (male, female and hermaphrodite) determined by a multiallelic locus. There are no morphological differences at the chromosome level; therefore the identification of sex types by chromosomal dimorphism is not possible. A RAPD marker of 900 bp was found in male plants, but not in females or hermaphrodites. From this RAPD marker a sequence characterized ampli-fied region (SCAR) was developed and it was possible to amplify fragments from the genomes of male and hermaphrodite plants, but not the female ones. The results indicate that this new SCAR marker will be valuable to determine the sex type of papaya plants.
La determinación del sexo en plantas de papaya es considerado un sistema intrigante, debido a que... more La determinación del sexo en plantas de papaya es considerado un sistema intrigante, debido a que esta especie presenta tres sexos (macho, hembra y hermafrodita) determinados por un locus multialélico. Además, esta especie no presenta cromosomas sexuales morfológicamente diferenciables. Los marcadores moleculares pueden asociarse a características de interés, como en el presente caso, al sexo en plántulas de papaya. Con el objetivo de identificar marcadores moleculares que permitan una rápida identificación del sexo en genotipos colombianos de plántulas de papaya, se aplicó la técnica RAPD (amplificación aleatoria de polimorfismos del ADN). El estudio encontró tres marcadores RAPD polimórficos, los cuales permitieron diferenciar los sexos de la papaya. Dos marcadores fueron específicos para plantas macho y hermafrodita, y un tercero para plantas hembra. Estos nuevos marcadores moleculares podrán ser beneficiosos en la determinación del sexo en genotipos colombianos de papaya.
A B S T R A C T In this study the molecular markers SCAR-SDSP, SCAR T1 and W11 -reported as sex d... more A B S T R A C T In this study the molecular markers SCAR-SDSP, SCAR T1 and W11 -reported as sex discriminators at seedling level-, were used to validate the effectiveness in flowered plants in the field. SCAR-SDSP amplified a band of 369 bp in male and hermaphrodite plants which allows corroborate the results obtained with this marker, developed at Corpoica using Colombian genotypes, in a previous study. With the use of the SCAR T1 and W11 markers combined in one reaction, it was possible to differentiate female and hermaphrodite plants. When the sex prediction was done with a blind sample using two months-old plantlets, from commercial seeds, a ratio of 1:1 female:hermaphrodite was observed, according to the segregation pattern of a cross between these two types of plants. The amplification with the SCAR W11 generated a band of 800 bp in male and hermaphrodite plants, independently of the sample's place of origin. However, when this band was sequenced and analyzed with the BLAST program, the analysis showed 98% identity between male and hermaphrodite sequence.
Sex type determination in papaya (Carica papaya L.) is very important for crop improvement proces... more Sex type determination in papaya (Carica papaya L.) is very important for crop improvement processes because it accelerates the identification of the fruitful plants. The use of molecular technology provides a quick and reliable identification of sex types in plantlets growing in seedbeds. Random amplified polymorphic DNA (RAPD) markers were used to determine the sex types of Colombian cultivars of dioecious papaya genotypes. This species has three sex types (male, female and hermaphrodite) determined by a multiallelic locus. There are no morphological differences at the chromosome level; therefore the identification of sex types by chromosomal dimorphism is not possible. A RAPD marker of 900 bp was found in male plants, but not in females or hermaphrodites. From this RAPD marker a sequence characterized ampli-fied region (SCAR) was developed and it was possible to amplify fragments from the genomes of male and hermaphrodite plants, but not the female ones. The results indicate that this new SCAR marker will be valuable to determine the sex type of papaya plants.