Jesús Mercado-Blanco | CSIC (Consejo Superior de Investigaciones Científicas-Spanish National Research Council) (original) (raw)
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Papers by Jesús Mercado-Blanco
Environmental microbiome, Mar 15, 2024
Background The complex and co-evolved interplay between plants and their microbiota is crucial fo... more Background The complex and co-evolved interplay between plants and their microbiota is crucial for the health and fitness of the plant holobiont. However, the microbiota of the seeds is still relatively unexplored and no studies have been conducted with olive trees so far. In this study, we aimed to characterize the bacterial, fungal and archaeal communities present in seeds of ten olive genotypes growing in the same orchard through amplicon sequencing to test whether the olive genotype is a major driver in shaping the seed microbial community, and to identify the origin of the latter. Therefore, we have developed a methodology for obtaining samples from the olive seed's endosphere under sterile conditions. Results A diverse microbiota was uncovered in olive seeds, the plant genotype being an important factor influencing the structure and composition of the microbial communities. The most abundant bacterial phylum was Actinobacteria, accounting for an average relative abundance of 41%. At genus level, Streptomyces stood out because of its potential influence on community structure. Within the fungal community, Basidiomycota and Ascomycota were the most abundant phyla, including the genera Malassezia, Cladosporium, and Mycosphaerella. The shared microbiome was composed of four bacterial (Stenotrophomonas, Streptomyces, Promicromonospora and Acidipropionibacterium) and three fungal (Malassezia, Cladosporium and Mycosphaerella) genera. Furthermore, a comparison between findings obtained here and earlier results from the root endosphere of the same trees indicated that genera such as Streptomyces and Malassezia were present in both olive compartments. Conclusions This study provides the first insights into the composition of the olive seed microbiota. The highly abundant fungal genus Malassezia and the bacterial genus Streptomyces reflect a unique signature of the olive seed microbiota. The genotype clearly shaped the composition of the seed's microbial community, although a shared microbiome was found. We identified genera that may translocate from the roots to the seeds, as they were present in both organs of the same trees. These findings set the stage for future research into potential vertical transmission of olive endophytes and the role of specific microbial taxa in seed germination, development, and seedling survival.
Specialty section: This article was submitted to
Poster presentado en el 28th New Phytologist Symposium. Functions and Ecology of the Plant Microb... more Poster presentado en el 28th New Phytologist Symposium. Functions and Ecology of the Plant Microbiome, celebrado en Rodas (Grecia) del 18 al 21 de mayo de 2012.
Trabajo presentado en la XII Reunion de Biologia Molecular de Plantas (RBMP), celebrado en Cartag... more Trabajo presentado en la XII Reunion de Biologia Molecular de Plantas (RBMP), celebrado en Cartagena del 11 al 13 de junio de 2014.
Frontiers in Plant Science, Jul 6, 2023
Trabajo presentado en el XL Congreso de la Sociedad Espanola de Genetica, celebrado en Cordoba de... more Trabajo presentado en el XL Congreso de la Sociedad Espanola de Genetica, celebrado en Cordoba del 16 al 18 de septiembre de 2015.
Frontiers in Microbiology, Aug 5, 2022
Designing a synthetic microbial community devoted to biological control: The case study of Fusari... more Designing a synthetic microbial community devoted to biological control: The case study of Fusarium wilt of banana.
Frontiers in Microbiology, Aug 28, 2019
Plant Pathology, Oct 1, 2001
This research was supported by grants P07-CVI-02624 from Junta de Andalucia and AGL2009-07275 fro... more This research was supported by grants P07-CVI-02624 from Junta de Andalucia and AGL2009-07275 from Spanish MICINN (co-financed by F EDER of the EU).
Frontiers Media SA eBooks, 2017
Journal of Molecular Biology, 1993
ABSTRACT
Standards in Genomic Sciences, Feb 6, 2015
Pseudomonas fluorescens strain PICF7 is a native endophyte of olive roots. Previous studies have ... more Pseudomonas fluorescens strain PICF7 is a native endophyte of olive roots. Previous studies have shown this motile, Gram-negative, non-sporulating bacterium is an effective biocontrol agent against the soil-borne fungus Verticillium dahliae, the causal agent of one of the most devastating diseases for olive (Olea europaea L.) cultivation. Here, we announce and describe the complete genome sequence of Pseudomonas fluorescens strain PICF7 consisting of a circular chromosome of 6,136,735 bp that encodes 5,567 protein-coding genes and 88 RNA-only encoding genes. Genome analysis revealed genes predicting factors such as secretion systems, siderophores, detoxifying compounds or volatile components. Further analysis of the genome sequence of PICF7 will help in gaining insights into biocontrol and endophytism.
Computational and Structural Biotechnology Journal
Environmental microbiome, Mar 15, 2024
Background The complex and co-evolved interplay between plants and their microbiota is crucial fo... more Background The complex and co-evolved interplay between plants and their microbiota is crucial for the health and fitness of the plant holobiont. However, the microbiota of the seeds is still relatively unexplored and no studies have been conducted with olive trees so far. In this study, we aimed to characterize the bacterial, fungal and archaeal communities present in seeds of ten olive genotypes growing in the same orchard through amplicon sequencing to test whether the olive genotype is a major driver in shaping the seed microbial community, and to identify the origin of the latter. Therefore, we have developed a methodology for obtaining samples from the olive seed's endosphere under sterile conditions. Results A diverse microbiota was uncovered in olive seeds, the plant genotype being an important factor influencing the structure and composition of the microbial communities. The most abundant bacterial phylum was Actinobacteria, accounting for an average relative abundance of 41%. At genus level, Streptomyces stood out because of its potential influence on community structure. Within the fungal community, Basidiomycota and Ascomycota were the most abundant phyla, including the genera Malassezia, Cladosporium, and Mycosphaerella. The shared microbiome was composed of four bacterial (Stenotrophomonas, Streptomyces, Promicromonospora and Acidipropionibacterium) and three fungal (Malassezia, Cladosporium and Mycosphaerella) genera. Furthermore, a comparison between findings obtained here and earlier results from the root endosphere of the same trees indicated that genera such as Streptomyces and Malassezia were present in both olive compartments. Conclusions This study provides the first insights into the composition of the olive seed microbiota. The highly abundant fungal genus Malassezia and the bacterial genus Streptomyces reflect a unique signature of the olive seed microbiota. The genotype clearly shaped the composition of the seed's microbial community, although a shared microbiome was found. We identified genera that may translocate from the roots to the seeds, as they were present in both organs of the same trees. These findings set the stage for future research into potential vertical transmission of olive endophytes and the role of specific microbial taxa in seed germination, development, and seedling survival.
Specialty section: This article was submitted to
Poster presentado en el 28th New Phytologist Symposium. Functions and Ecology of the Plant Microb... more Poster presentado en el 28th New Phytologist Symposium. Functions and Ecology of the Plant Microbiome, celebrado en Rodas (Grecia) del 18 al 21 de mayo de 2012.
Trabajo presentado en la XII Reunion de Biologia Molecular de Plantas (RBMP), celebrado en Cartag... more Trabajo presentado en la XII Reunion de Biologia Molecular de Plantas (RBMP), celebrado en Cartagena del 11 al 13 de junio de 2014.
Frontiers in Plant Science, Jul 6, 2023
Trabajo presentado en el XL Congreso de la Sociedad Espanola de Genetica, celebrado en Cordoba de... more Trabajo presentado en el XL Congreso de la Sociedad Espanola de Genetica, celebrado en Cordoba del 16 al 18 de septiembre de 2015.
Frontiers in Microbiology, Aug 5, 2022
Designing a synthetic microbial community devoted to biological control: The case study of Fusari... more Designing a synthetic microbial community devoted to biological control: The case study of Fusarium wilt of banana.
Frontiers in Microbiology, Aug 28, 2019
Plant Pathology, Oct 1, 2001
This research was supported by grants P07-CVI-02624 from Junta de Andalucia and AGL2009-07275 fro... more This research was supported by grants P07-CVI-02624 from Junta de Andalucia and AGL2009-07275 from Spanish MICINN (co-financed by F EDER of the EU).
Frontiers Media SA eBooks, 2017
Journal of Molecular Biology, 1993
ABSTRACT
Standards in Genomic Sciences, Feb 6, 2015
Pseudomonas fluorescens strain PICF7 is a native endophyte of olive roots. Previous studies have ... more Pseudomonas fluorescens strain PICF7 is a native endophyte of olive roots. Previous studies have shown this motile, Gram-negative, non-sporulating bacterium is an effective biocontrol agent against the soil-borne fungus Verticillium dahliae, the causal agent of one of the most devastating diseases for olive (Olea europaea L.) cultivation. Here, we announce and describe the complete genome sequence of Pseudomonas fluorescens strain PICF7 consisting of a circular chromosome of 6,136,735 bp that encodes 5,567 protein-coding genes and 88 RNA-only encoding genes. Genome analysis revealed genes predicting factors such as secretion systems, siderophores, detoxifying compounds or volatile components. Further analysis of the genome sequence of PICF7 will help in gaining insights into biocontrol and endophytism.
Computational and Structural Biotechnology Journal