Ravi Pulla | JNTU , hyderabad (original) (raw)

Papers by Ravi Pulla

British journal of pharmaceutical research, Jan 10, 2016

Purpose: To develop a highly selective, reproducible & precise rugged bio analytical method for e... more Purpose: To develop a highly selective, reproducible & precise rugged bio analytical method for estimation of Bortezomib (BTZ), "A Protease Inhibitor" in human plasma by validating the developed method in accordance to US-FDA guidelines. Methodology Envisaged: BTZ D 3 was used as an internal standard (ISTD) for the determination of BTZ in human plasma using a rapid & specific liquid chromatographic-Electron Spray Ionization-Mass spectrometric method. The analytical method was moduled with liquid-liquid phase extraction by using annular centrifugal contactor & the samples were analyzed by HPLC, on a column-ACE 5CN (150 x 4.6 mm 5 µm), using mobile phase consisting of ammonium formate buffer: ACN (25:75 v/v), delivered at 1.0 ml/min & 90% flow spitting. Applied Bio system MDS Sciex API 3000 Triple Quadruple MS equipped with Turbo Ion Spray (TIS) as LC/MS interface was used in for MS detection. TIS with multiple reaction monitoring (MRM) were acquired by ESI mass spectra, using the transitions m/z 362.95→310.21 & m/z 172.64→146.06 to quantify BTZ & BTZ D 3 respectively. Original Research Article Results: % variability was ≤ 5.52 & ≤ 6.15 [that showing no matrix interferences across the elution system. Acceptance is ranging between 2.83 &-4.32 to 1.00% (< 5% CV) & accuracy in the range of 92.73 was observed over a linear range of 2.00 was 0.9991 & overall mean recovery was 85.62%. Retention time for drug & ISTD is found out to be 0.08 & 0.07; % CV of area ratio is 1.91% & area ratio suitability. Interpretation and Conclusion: stability experimentation & within the acceptance limits. It can be used for investigating drug concentration in routine quality control ana

A simple, precise, rapid and accurate RPHPLC method was developed for the estimation of Alosetron... more A simple, precise, rapid and accurate RPHPLC method was developed for the estimation of Alosetron Hydrochloride (AST) in tablet dosage forms. Waters Spherisorb ® 5μm CN, 250x4.6 mm, column with 5 μm particle size and the Mobile Phase consisting of 0.01M Ammonium Acetate in water adjusting the pH-3.2 with Methanol & Tetra Hydro Furan in ratio of 700:240:60 v/v & mobile phase used as a diluent in the gradient mode. The flow rate was 1.0 ml/min and the effluents were monitored at 295 nm. The retention time was 11.570 min and the detector response was linear in the concentration range of 56.1673.2 μg/mL for AST successively. The respective linear regression equation being Y= 27228.755x + 221036.4041 for AST. The Limit of Detection (LOD) & The Limit of Quantification (LOQ) was found to be 0.1 & 0.3 μg/mL for AST. The percentage assay of AST was 99.93%. The method was validated by determining its accuracy, precision and system suitability. The results of the study showed that the proposed...

A simple, precise, rapid and accurate Reverse Phase HPLC method was developed for the estimation ... more A simple, precise, rapid and accurate Reverse Phase HPLC method was developed for the estimation of Prasugrel in tablet dosage form. An Xterra RP C18, 250x4.6 mm, column with 5 µm particle size and the mobile phase consisting of 0.03M K2HPO4 in water pH: 3.2 adjusted with Acetonitrile (25:75). Acetonitrile in the isocratic mode was used. The flow rate was 1.0 ml/min and the effluents were monitored at 210 nm. The retention time was 4.762 min. The detector response was linear in the concentration of 50-600 mcg/mL for Prasugrel. The respective linear regression equation being Y (158660.8513) = 10312.358x + 237007.9858 for Prasugrel. The Limit of Detection (LOD) and The Limit of Quantification (LOQ) were 2.5 mcg and 7.5 mcg respectively for Prasugrel. The percentage assay of Prasugrel was 99.93 %. The method was validated by determining its accuracy, precision and system suitability. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate...

Acta Chromatographica, 2014

The objective of the present study was to report the stability of Simvastatin (SMV), a HMG-CoA re... more The objective of the present study was to report the stability of Simvastatin (SMV), a HMG-CoA reductase inhibitor, based on forced degradation studies. SMV was subjected to forced hydrolytic (acidic, alkaline and neutral), oxidative, photolytic and thermal stress in accordance with the ICH guideline Q1A (R 2). SMV was found to degrade significantly in all stress conditions except photo degradation. Resolution of the drug and degradation products was achieved on a Hi-Q Sil C-18 column (4.6 × 250 mm, 5 μm) utilizing acetonitrile, methanol and phosphate buffer (65:25:10% v/v/v) of pH 4 at a flow rate of 1.2 ml/min and at the detection wavelength 237 nm. The major acidic stress degradation product was characterized by LC-ESI-MS/MS and its fragmentation pathway was proposed. Validation of the liquid chromatographic (LC) method was carried out in accordance with ICH guidelines. The method met all required criteria and was applied for analysis of commercially available tablets viz. Simvas 40.

Biomedical Chromatography, 2007

New bioanalytical methods have been developed for the determination of imidafenacin (KRP-197/ONO-... more New bioanalytical methods have been developed for the determination of imidafenacin (KRP-197/ONO-8025, IM), a novel antimuscarinic drug developed for the treatment of overactive bladder, and its metabolites, M-2, M-3, M-4, M-6 and M-8 (method 1), M-5 and M-9 (method 2) in human urine by using liquid chromatography-tandem mass spectrometry. In each method, the urine sample was extracted by solid-phase extraction, separated on a semi-micro high-performance liquid chromatography column using gradient elution and detected by tandem mass spectrometer with an atmospheric pressure chemical ionization or ionspray interface. Extraction recoveries of IM and metabolites were 81.4% or more. Calibration curves had good linearity in the concentration ranges 0.2-50 ng/mL for IM, M-2, M-3, M-4, M-6 and M-8 (method 1) and 1-250 ng/mL for M-5 and M-9 (method 2), respectively. The accuracy and precision in the intra-day and inter-day reproducibility tests were within +/-17.0 and 16.1% at the lowest concentrations, and within +/-12.8 and 11.1% at higher concentrations, respectively. Using these analytical methods, excretion profiles of IM and its metabolites in human urine were successfully determined after oral administration of IM at the therapeutic dosage of 0.1 mg.

A simple, precise, rapid and accurate Reverse Phase HPLC method was developed for the estimation ... more A simple, precise, rapid and accurate Reverse Phase HPLC method was developed for the estimation of Troxipide in tablet dosage form. An Inertsil ODS-3V, 250x4.6 mm, column with 5 µm particle size and the mobile phase consisting of 0.03M Ammonium Acetate in water pH: 3.2 adjusted with Acetonitrile (50:50). Acetonitrile in the isocratic mode was used. The flow rate was 0.8 ml/min and the effluents were monitored at 212 nm. The retention time was 3.046 min. The detector response was linear in the concentration of 10-120 mcg/mL for Troxipide. The respective linear regression equation being Y (-105593.8824) = 65647.112x + 45974.1647 for Troxipide. The Limit of Detection (LOD) and The Limit of Quantification (LOQ) were 1 mcg/mL and 3 mcg/mL respectively for Troxipide. The percentage assay of Troxipide was 98.17 %. The method was validated by determining its accuracy, precision and system suitability. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precis...

In the modern era, technology has taken a radical avant-garde towards social media. It is present... more In the modern era, technology has taken a radical avant-garde towards social media. It is presently being used as a stature household need by every mankind. The revolutionary of social media has brought a dynamic impact in networking health information between the professional and patient, irrespective of age group. This laid a "bleeding-heart" platform for pharmacist, escalating the utilization by improving the health outcomes and meeting the urge. There is a vague informative literature around this upcoming subject. To bridge this gap, the authors reconnoitre the positive potential areas concentrating on the use and applications of social media by pharmacist to improvise & reinvigorate determinants of health outcomes. The subject was extensively studied in five main modules. 1. Encapsulating the complete medical information 2. Innocuous usage of medicine 3. In perpetuity medication usage 4. Document based medicine & implementation guidelines & 5. Clinical inquisition. In...

www.ijcas.info *Corresponding author.

Research Journal of Pharmacy and Technology, 2011

A simple, precise, rapid and accurate Reverse Phase HPLC method was developed for the estimation ... more A simple, precise, rapid and accurate Reverse Phase HPLC method was developed for the estimation of Teicoplanin in Parenteral dosage form. An Xterra RP C18, 250x4.6 mm, column with 5 μm particle size and the mobile phase consisting of 0.02M Potassium Dihydrogen Orthophosphate + 0.02M Dipotassium Hydrogen Orthophosphate + 0.5% Triethyl Amine in water pH: 3.2 adjusted with Orthophosphoric Acid: Acetonitrile (40:50). Acetonitrile in the isocratic mode was used. The flow rate was 0.5 ml/min and the effluents were monitored at 210 nm. The retention time was 4.178 min. The detector response was linear in the concentration of 24.1-289.2 mcg/mL for Teicoplanin, the respective linear regression equation being Y (-242937.9235) = 71026.949x + 174544.8363 for Teicoplanin. The Limit of Detection (LOD) and The Limit of Quantification (LOQ) were 1.205 mcg/mL and 3.615 mcg/mL respectively for Teicoplanin. The percentage assay of Teicoplanin was 99.64 %. The method was validated by determining its a...

A simple, accurate and sensitive liquid chromatographic method has been developed for the determi... more A simple, accurate and sensitive liquid chromatographic method has been developed for the determination of Ibandronic Acid (IBN) in tablet dosage form. The separation was achieved on Inertsil ODS (250 mm X 4.6 mm) C18, 5μm column. The mobile phase consists of Buffer: ACN (50:50 v/v); Flow rate 0.8ml min−1 at ambient temperature. The analytes were monitored by UV detector. The method was validated for specificity, precision, linearity, and accuracy. The average recoveries for Ibandronic Acid were in the range of 99.0–102.0% and the method can be successfully applied for the routine analysis of the drug substance.

An accurate, sensitive, precise, rapid and isocratic Reversed-Phase HPLC, (RP-HPLC) method for si... more An accurate, sensitive, precise, rapid and isocratic Reversed-Phase HPLC, (RP-HPLC) method for simultaneous estimation of Formoterol Fumarate, Tiotropium Bromide and Ciclesonide in the bulk drug and in Pharmaceutical metered dose inhalers has been developed and validated. The best separation was achieved on a 250 mm × 4.6 mm i.d., 5-μm particle, Inertsil ODS 3V-RP C18 column with Acetonitrile as the organic modifier and Di-Potassium Hydrogen Phosphate [0.03M] in water with pH 3.2 adjusted with Ortho-Phosphoric Acid (0.1% v/v) in the proportion of [30:70 v/v] as mobile phase at a flow rate of 0.8 mL min−1. UV detection was at 254 nm. Retention times were found to be 5.206 min. for Formoterol Fumarate, 7.016 min. for Tiotropium Bromide and 9.991 min for Ciclesonide. The response was a linear function of concentration over the range of 0.72–8.64 mcg/ml, 1.08–12.96 mcg/ml and 24–288 mcg/ml respectively with correlation coefficient of 0.9999. The percentage assay Formoterol Fumarate, Tio...

A simple, economical, precise, reliable and reproducible visible spectrophotometric method has be... more A simple, economical, precise, reliable and reproducible visible spectrophotometric method has been developed for the estimation of Doxorubicin HCl (DXR) in bulk as well as in parenteral formulation. The developed method was based on the formation of a yellow color complex between DXR and Bromo Thymol Blue (BTB) in the presence of Phosphate buffer which shows maximum absorption at 414 nm. The absorbance-concentration plot is linear over the range 10-50 µg/mL. The different experimental parameters effecting the development and stability were studied carefully and optimized. A result of analysis for the method was validated statistically and recovery studies were also performed.

A simple, accurate and sensitive liquid chromatographic method has been developed for the determi... more A simple, accurate and sensitive liquid chromatographic method has been developed for the determination of Rasagiline Tartrate (RAST) in API and its dosage forms. The separation was achieved on Inertsil ODS (250mm X 4.6mm) C18, 5 μm column. The mobile phase consisted of Methanol: Acetonitrile: Water (50:30:20 v/v), pH: 3.2 adjusted with Triethylamine (TEA), flow rate 1 mL/min at ambient temperature. The analytes were monitored by PDA detector at 206 nm. The method was validated for specificity, precision, linearity, and accuracy. The average recoveries for RAST were in the range of 89.0 – 93.0% and the present work has been focused on the development of simple, accurate and precise analytical method based on RP-HPLC for the formulation of RAST.

A simple, precise, rapid and accurate RP- HPLC method was developed for the estimation of Emtrice... more A simple, precise, rapid and accurate RP- HPLC method was developed for the estimation of Emtricetabine (FTC), Tenofovir Disoproxil Fumarate (TDF) and Rilpivirine HCl (RPV)in tablet dosage forms. An Inertsil ODS 3V, 250x4.6 mm, column with 5 μm particle size and the Mobile Phase- A, consisting of 0.03M KH2PO4 in water adjusting the pH-3.2 with dilute O-Phosphoric Acid, Mobile Phase-B consisting of Methanol & Water in ratio of 85:15 v/v & Acetonitrile & Buffer in ratio of 70:30 v/v, was used as diluent in the gradient mode. The flow rate was 1.5 ml/min and the effluents were monitored at 265 nm. The retention times were 6.250 min for FTC, 8.386 min for TDF and 10.296 min for RPV successively. The detector response was linear in the concentration of 80-960 µg/mL for FTC, 120-1440 µg/mL for TDF and 10-120 µg/mL for RPV. The respective linear regression equation being Y= 9474.289x + 147734.8116 for FTC, Y = 6903.437x + 202292.0234 for TDF and Y= 25680.392x + 15736.147 for RPV. The Limit...

Two simple, accurate, rapid and sensitive Methods (A and B) have been developed for the estimatio... more Two simple, accurate, rapid and sensitive Methods (A and B) have been developed for the estimation of Alosetron in its pharmaceutical dosage form. The Method A is based on the formation yellow colored chromogen, due to reaction of Alosetron Hydrochloride with Metanil yellow dye, formation of ion association complexes of the drug with dyes in phosphate buffer of pH 3.6 followed by their extraction in chloroform which exhibits λmax at 410 nm. The Method B is based on the formation of light yellow colored chromogen due to reaction of Alosetron Hydrochloride with Methyl Orange dye, formation of ion association complexes of the drug with dyes in phosphate buffer of pH 3.6 followed by their extraction in chloroform, which exhibits λ max at 422 nm. The absorbance-concentration plot is linear over the range of 5-60 mcg/mL for Method A and 50-120 mcg/mL for Method B. Results of analysis for all the methods were validated statistically and by recovery studies. The proposed methods are precise...

IOSR Journal of Pharmacy and Biological Sciences, 2012

Two simple, accurate, sensitive and reproducible visible spectrophotometric methods (A & B) have ... more Two simple, accurate, sensitive and reproducible visible spectrophotometric methods (A & B) have been developed for the determination of Sitagliptin Phosphate (SGP) in bulk and also in pharmaceutical formulations. The proposed methods are based on complexation of the drug with Bromo Thymol Blue (BTB-Method A) & Bromo Cresol Green (BCG-Method B), extracted with chloroform, showing absorbance maxima at 412 nm and 419 nm respectively. Beer's law is obeyed over a concentration range of 25-125 µg/ml and 10-50 µg/ml respectively. Results of analysis for the two methods established, were validated statistically and also by recovery studies. All the variables were studied to optimize the reaction conditions. No interference was observed in the presence of common pharmaceutical excipients. The validity of the methods was tested by analyzing the drug in its pharmaceutical preparations. Good recoveries were obtained. The developed methods employed were successful for the determination of Sitagliptin Phosphate in various pharmaceutical preparations.

A simple, precise, rapid and accurate RP- HPLC method was developed for the estimation of Alosetr... more A simple, precise, rapid and accurate RP- HPLC method was developed for the estimation of Alosetron Hydrochloride (AST) in tablet dosage forms. Waters Spherisorb® 5μm CN, 250x4.6 mm, column with 5 μm particle size and the Mobile Phase consisting of 0.01M Ammonium Acetate in water adjusting the pH-3.2 with Methanol & Tetra Hydro Furan in ratio of 700:240:60 v/v & mobile phase used as a diluent in the gradient mode. The flow rate was 1.0 ml/min and the effluents were monitored at 295 nm. The retention time was 11.570 min and the detector response was linear in the concentration range of 56.1- 673.2 μg/mL for AST successively. The respective linear regression equation being Y= 27228.755x + 221036.4041 for AST. The Limit of Detection (LOD) & The Limit of Quantification (LOQ) was found to be 0.1 & 0.3 μg/mL for AST. The percentage assay of AST was 99.93%. The method was validated by determining its accuracy, precision and system suitability. The results of the study showed that the propo...

In the modern era, technology has taken a radical avant-garde towards social media. It is present... more In the modern era, technology has taken a radical avant-garde towards social media. It is presently being used as a stature household need by every mankind. The revolutionary of social media has brought a dynamic impact in networking health information between the professional and patient, irrespective of age group. This laid a “bleeding-heart” platform for pharmacist, escalating the utilization by improving the health outcomes and meeting the urge. There is a vague informative literature around this upcoming subject. To bridge this gap, the authors reconnoitre the positive potential areas concentrating on the use and applications of social media by pharmacist to improvise & reinvigorate determinants of health outcomes. The subject was extensively studied in five main modules. 1. Encapsulating the complete medical information 2. Innocuous usage of medicine 3. In perpetuity medication usage 4. Document based medicine & implementation guidelines & 5. Clinical inquisition. In each of t...

British journal of pharmaceutical research, Jan 10, 2016

Purpose: To develop a highly selective, reproducible & precise rugged bio analytical method for e... more Purpose: To develop a highly selective, reproducible & precise rugged bio analytical method for estimation of Bortezomib (BTZ), "A Protease Inhibitor" in human plasma by validating the developed method in accordance to US-FDA guidelines. Methodology Envisaged: BTZ D 3 was used as an internal standard (ISTD) for the determination of BTZ in human plasma using a rapid & specific liquid chromatographic-Electron Spray Ionization-Mass spectrometric method. The analytical method was moduled with liquid-liquid phase extraction by using annular centrifugal contactor & the samples were analyzed by HPLC, on a column-ACE 5CN (150 x 4.6 mm 5 µm), using mobile phase consisting of ammonium formate buffer: ACN (25:75 v/v), delivered at 1.0 ml/min & 90% flow spitting. Applied Bio system MDS Sciex API 3000 Triple Quadruple MS equipped with Turbo Ion Spray (TIS) as LC/MS interface was used in for MS detection. TIS with multiple reaction monitoring (MRM) were acquired by ESI mass spectra, using the transitions m/z 362.95→310.21 & m/z 172.64→146.06 to quantify BTZ & BTZ D 3 respectively. Original Research Article Results: % variability was ≤ 5.52 & ≤ 6.15 [that showing no matrix interferences across the elution system. Acceptance is ranging between 2.83 &-4.32 to 1.00% (< 5% CV) & accuracy in the range of 92.73 was observed over a linear range of 2.00 was 0.9991 & overall mean recovery was 85.62%. Retention time for drug & ISTD is found out to be 0.08 & 0.07; % CV of area ratio is 1.91% & area ratio suitability. Interpretation and Conclusion: stability experimentation & within the acceptance limits. It can be used for investigating drug concentration in routine quality control ana

A simple, precise, rapid and accurate RPHPLC method was developed for the estimation of Alosetron... more A simple, precise, rapid and accurate RPHPLC method was developed for the estimation of Alosetron Hydrochloride (AST) in tablet dosage forms. Waters Spherisorb ® 5μm CN, 250x4.6 mm, column with 5 μm particle size and the Mobile Phase consisting of 0.01M Ammonium Acetate in water adjusting the pH-3.2 with Methanol & Tetra Hydro Furan in ratio of 700:240:60 v/v & mobile phase used as a diluent in the gradient mode. The flow rate was 1.0 ml/min and the effluents were monitored at 295 nm. The retention time was 11.570 min and the detector response was linear in the concentration range of 56.1673.2 μg/mL for AST successively. The respective linear regression equation being Y= 27228.755x + 221036.4041 for AST. The Limit of Detection (LOD) & The Limit of Quantification (LOQ) was found to be 0.1 & 0.3 μg/mL for AST. The percentage assay of AST was 99.93%. The method was validated by determining its accuracy, precision and system suitability. The results of the study showed that the proposed...

A simple, precise, rapid and accurate Reverse Phase HPLC method was developed for the estimation ... more A simple, precise, rapid and accurate Reverse Phase HPLC method was developed for the estimation of Prasugrel in tablet dosage form. An Xterra RP C18, 250x4.6 mm, column with 5 µm particle size and the mobile phase consisting of 0.03M K2HPO4 in water pH: 3.2 adjusted with Acetonitrile (25:75). Acetonitrile in the isocratic mode was used. The flow rate was 1.0 ml/min and the effluents were monitored at 210 nm. The retention time was 4.762 min. The detector response was linear in the concentration of 50-600 mcg/mL for Prasugrel. The respective linear regression equation being Y (158660.8513) = 10312.358x + 237007.9858 for Prasugrel. The Limit of Detection (LOD) and The Limit of Quantification (LOQ) were 2.5 mcg and 7.5 mcg respectively for Prasugrel. The percentage assay of Prasugrel was 99.93 %. The method was validated by determining its accuracy, precision and system suitability. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate...

Acta Chromatographica, 2014

The objective of the present study was to report the stability of Simvastatin (SMV), a HMG-CoA re... more The objective of the present study was to report the stability of Simvastatin (SMV), a HMG-CoA reductase inhibitor, based on forced degradation studies. SMV was subjected to forced hydrolytic (acidic, alkaline and neutral), oxidative, photolytic and thermal stress in accordance with the ICH guideline Q1A (R 2). SMV was found to degrade significantly in all stress conditions except photo degradation. Resolution of the drug and degradation products was achieved on a Hi-Q Sil C-18 column (4.6 × 250 mm, 5 μm) utilizing acetonitrile, methanol and phosphate buffer (65:25:10% v/v/v) of pH 4 at a flow rate of 1.2 ml/min and at the detection wavelength 237 nm. The major acidic stress degradation product was characterized by LC-ESI-MS/MS and its fragmentation pathway was proposed. Validation of the liquid chromatographic (LC) method was carried out in accordance with ICH guidelines. The method met all required criteria and was applied for analysis of commercially available tablets viz. Simvas 40.

Biomedical Chromatography, 2007

New bioanalytical methods have been developed for the determination of imidafenacin (KRP-197/ONO-... more New bioanalytical methods have been developed for the determination of imidafenacin (KRP-197/ONO-8025, IM), a novel antimuscarinic drug developed for the treatment of overactive bladder, and its metabolites, M-2, M-3, M-4, M-6 and M-8 (method 1), M-5 and M-9 (method 2) in human urine by using liquid chromatography-tandem mass spectrometry. In each method, the urine sample was extracted by solid-phase extraction, separated on a semi-micro high-performance liquid chromatography column using gradient elution and detected by tandem mass spectrometer with an atmospheric pressure chemical ionization or ionspray interface. Extraction recoveries of IM and metabolites were 81.4% or more. Calibration curves had good linearity in the concentration ranges 0.2-50 ng/mL for IM, M-2, M-3, M-4, M-6 and M-8 (method 1) and 1-250 ng/mL for M-5 and M-9 (method 2), respectively. The accuracy and precision in the intra-day and inter-day reproducibility tests were within +/-17.0 and 16.1% at the lowest concentrations, and within +/-12.8 and 11.1% at higher concentrations, respectively. Using these analytical methods, excretion profiles of IM and its metabolites in human urine were successfully determined after oral administration of IM at the therapeutic dosage of 0.1 mg.

A simple, precise, rapid and accurate Reverse Phase HPLC method was developed for the estimation ... more A simple, precise, rapid and accurate Reverse Phase HPLC method was developed for the estimation of Troxipide in tablet dosage form. An Inertsil ODS-3V, 250x4.6 mm, column with 5 µm particle size and the mobile phase consisting of 0.03M Ammonium Acetate in water pH: 3.2 adjusted with Acetonitrile (50:50). Acetonitrile in the isocratic mode was used. The flow rate was 0.8 ml/min and the effluents were monitored at 212 nm. The retention time was 3.046 min. The detector response was linear in the concentration of 10-120 mcg/mL for Troxipide. The respective linear regression equation being Y (-105593.8824) = 65647.112x + 45974.1647 for Troxipide. The Limit of Detection (LOD) and The Limit of Quantification (LOQ) were 1 mcg/mL and 3 mcg/mL respectively for Troxipide. The percentage assay of Troxipide was 98.17 %. The method was validated by determining its accuracy, precision and system suitability. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precis...

In the modern era, technology has taken a radical avant-garde towards social media. It is present... more In the modern era, technology has taken a radical avant-garde towards social media. It is presently being used as a stature household need by every mankind. The revolutionary of social media has brought a dynamic impact in networking health information between the professional and patient, irrespective of age group. This laid a "bleeding-heart" platform for pharmacist, escalating the utilization by improving the health outcomes and meeting the urge. There is a vague informative literature around this upcoming subject. To bridge this gap, the authors reconnoitre the positive potential areas concentrating on the use and applications of social media by pharmacist to improvise & reinvigorate determinants of health outcomes. The subject was extensively studied in five main modules. 1. Encapsulating the complete medical information 2. Innocuous usage of medicine 3. In perpetuity medication usage 4. Document based medicine & implementation guidelines & 5. Clinical inquisition. In...

www.ijcas.info *Corresponding author.

Research Journal of Pharmacy and Technology, 2011

A simple, precise, rapid and accurate Reverse Phase HPLC method was developed for the estimation ... more A simple, precise, rapid and accurate Reverse Phase HPLC method was developed for the estimation of Teicoplanin in Parenteral dosage form. An Xterra RP C18, 250x4.6 mm, column with 5 μm particle size and the mobile phase consisting of 0.02M Potassium Dihydrogen Orthophosphate + 0.02M Dipotassium Hydrogen Orthophosphate + 0.5% Triethyl Amine in water pH: 3.2 adjusted with Orthophosphoric Acid: Acetonitrile (40:50). Acetonitrile in the isocratic mode was used. The flow rate was 0.5 ml/min and the effluents were monitored at 210 nm. The retention time was 4.178 min. The detector response was linear in the concentration of 24.1-289.2 mcg/mL for Teicoplanin, the respective linear regression equation being Y (-242937.9235) = 71026.949x + 174544.8363 for Teicoplanin. The Limit of Detection (LOD) and The Limit of Quantification (LOQ) were 1.205 mcg/mL and 3.615 mcg/mL respectively for Teicoplanin. The percentage assay of Teicoplanin was 99.64 %. The method was validated by determining its a...

A simple, accurate and sensitive liquid chromatographic method has been developed for the determi... more A simple, accurate and sensitive liquid chromatographic method has been developed for the determination of Ibandronic Acid (IBN) in tablet dosage form. The separation was achieved on Inertsil ODS (250 mm X 4.6 mm) C18, 5μm column. The mobile phase consists of Buffer: ACN (50:50 v/v); Flow rate 0.8ml min−1 at ambient temperature. The analytes were monitored by UV detector. The method was validated for specificity, precision, linearity, and accuracy. The average recoveries for Ibandronic Acid were in the range of 99.0–102.0% and the method can be successfully applied for the routine analysis of the drug substance.

An accurate, sensitive, precise, rapid and isocratic Reversed-Phase HPLC, (RP-HPLC) method for si... more An accurate, sensitive, precise, rapid and isocratic Reversed-Phase HPLC, (RP-HPLC) method for simultaneous estimation of Formoterol Fumarate, Tiotropium Bromide and Ciclesonide in the bulk drug and in Pharmaceutical metered dose inhalers has been developed and validated. The best separation was achieved on a 250 mm × 4.6 mm i.d., 5-μm particle, Inertsil ODS 3V-RP C18 column with Acetonitrile as the organic modifier and Di-Potassium Hydrogen Phosphate [0.03M] in water with pH 3.2 adjusted with Ortho-Phosphoric Acid (0.1% v/v) in the proportion of [30:70 v/v] as mobile phase at a flow rate of 0.8 mL min−1. UV detection was at 254 nm. Retention times were found to be 5.206 min. for Formoterol Fumarate, 7.016 min. for Tiotropium Bromide and 9.991 min for Ciclesonide. The response was a linear function of concentration over the range of 0.72–8.64 mcg/ml, 1.08–12.96 mcg/ml and 24–288 mcg/ml respectively with correlation coefficient of 0.9999. The percentage assay Formoterol Fumarate, Tio...

A simple, economical, precise, reliable and reproducible visible spectrophotometric method has be... more A simple, economical, precise, reliable and reproducible visible spectrophotometric method has been developed for the estimation of Doxorubicin HCl (DXR) in bulk as well as in parenteral formulation. The developed method was based on the formation of a yellow color complex between DXR and Bromo Thymol Blue (BTB) in the presence of Phosphate buffer which shows maximum absorption at 414 nm. The absorbance-concentration plot is linear over the range 10-50 µg/mL. The different experimental parameters effecting the development and stability were studied carefully and optimized. A result of analysis for the method was validated statistically and recovery studies were also performed.

A simple, accurate and sensitive liquid chromatographic method has been developed for the determi... more A simple, accurate and sensitive liquid chromatographic method has been developed for the determination of Rasagiline Tartrate (RAST) in API and its dosage forms. The separation was achieved on Inertsil ODS (250mm X 4.6mm) C18, 5 μm column. The mobile phase consisted of Methanol: Acetonitrile: Water (50:30:20 v/v), pH: 3.2 adjusted with Triethylamine (TEA), flow rate 1 mL/min at ambient temperature. The analytes were monitored by PDA detector at 206 nm. The method was validated for specificity, precision, linearity, and accuracy. The average recoveries for RAST were in the range of 89.0 – 93.0% and the present work has been focused on the development of simple, accurate and precise analytical method based on RP-HPLC for the formulation of RAST.

A simple, precise, rapid and accurate RP- HPLC method was developed for the estimation of Emtrice... more A simple, precise, rapid and accurate RP- HPLC method was developed for the estimation of Emtricetabine (FTC), Tenofovir Disoproxil Fumarate (TDF) and Rilpivirine HCl (RPV)in tablet dosage forms. An Inertsil ODS 3V, 250x4.6 mm, column with 5 μm particle size and the Mobile Phase- A, consisting of 0.03M KH2PO4 in water adjusting the pH-3.2 with dilute O-Phosphoric Acid, Mobile Phase-B consisting of Methanol & Water in ratio of 85:15 v/v & Acetonitrile & Buffer in ratio of 70:30 v/v, was used as diluent in the gradient mode. The flow rate was 1.5 ml/min and the effluents were monitored at 265 nm. The retention times were 6.250 min for FTC, 8.386 min for TDF and 10.296 min for RPV successively. The detector response was linear in the concentration of 80-960 µg/mL for FTC, 120-1440 µg/mL for TDF and 10-120 µg/mL for RPV. The respective linear regression equation being Y= 9474.289x + 147734.8116 for FTC, Y = 6903.437x + 202292.0234 for TDF and Y= 25680.392x + 15736.147 for RPV. The Limit...

Two simple, accurate, rapid and sensitive Methods (A and B) have been developed for the estimatio... more Two simple, accurate, rapid and sensitive Methods (A and B) have been developed for the estimation of Alosetron in its pharmaceutical dosage form. The Method A is based on the formation yellow colored chromogen, due to reaction of Alosetron Hydrochloride with Metanil yellow dye, formation of ion association complexes of the drug with dyes in phosphate buffer of pH 3.6 followed by their extraction in chloroform which exhibits λmax at 410 nm. The Method B is based on the formation of light yellow colored chromogen due to reaction of Alosetron Hydrochloride with Methyl Orange dye, formation of ion association complexes of the drug with dyes in phosphate buffer of pH 3.6 followed by their extraction in chloroform, which exhibits λ max at 422 nm. The absorbance-concentration plot is linear over the range of 5-60 mcg/mL for Method A and 50-120 mcg/mL for Method B. Results of analysis for all the methods were validated statistically and by recovery studies. The proposed methods are precise...

IOSR Journal of Pharmacy and Biological Sciences, 2012

Two simple, accurate, sensitive and reproducible visible spectrophotometric methods (A & B) have ... more Two simple, accurate, sensitive and reproducible visible spectrophotometric methods (A & B) have been developed for the determination of Sitagliptin Phosphate (SGP) in bulk and also in pharmaceutical formulations. The proposed methods are based on complexation of the drug with Bromo Thymol Blue (BTB-Method A) & Bromo Cresol Green (BCG-Method B), extracted with chloroform, showing absorbance maxima at 412 nm and 419 nm respectively. Beer's law is obeyed over a concentration range of 25-125 µg/ml and 10-50 µg/ml respectively. Results of analysis for the two methods established, were validated statistically and also by recovery studies. All the variables were studied to optimize the reaction conditions. No interference was observed in the presence of common pharmaceutical excipients. The validity of the methods was tested by analyzing the drug in its pharmaceutical preparations. Good recoveries were obtained. The developed methods employed were successful for the determination of Sitagliptin Phosphate in various pharmaceutical preparations.

A simple, precise, rapid and accurate RP- HPLC method was developed for the estimation of Alosetr... more A simple, precise, rapid and accurate RP- HPLC method was developed for the estimation of Alosetron Hydrochloride (AST) in tablet dosage forms. Waters Spherisorb® 5μm CN, 250x4.6 mm, column with 5 μm particle size and the Mobile Phase consisting of 0.01M Ammonium Acetate in water adjusting the pH-3.2 with Methanol & Tetra Hydro Furan in ratio of 700:240:60 v/v & mobile phase used as a diluent in the gradient mode. The flow rate was 1.0 ml/min and the effluents were monitored at 295 nm. The retention time was 11.570 min and the detector response was linear in the concentration range of 56.1- 673.2 μg/mL for AST successively. The respective linear regression equation being Y= 27228.755x + 221036.4041 for AST. The Limit of Detection (LOD) & The Limit of Quantification (LOQ) was found to be 0.1 & 0.3 μg/mL for AST. The percentage assay of AST was 99.93%. The method was validated by determining its accuracy, precision and system suitability. The results of the study showed that the propo...

In the modern era, technology has taken a radical avant-garde towards social media. It is present... more In the modern era, technology has taken a radical avant-garde towards social media. It is presently being used as a stature household need by every mankind. The revolutionary of social media has brought a dynamic impact in networking health information between the professional and patient, irrespective of age group. This laid a “bleeding-heart” platform for pharmacist, escalating the utilization by improving the health outcomes and meeting the urge. There is a vague informative literature around this upcoming subject. To bridge this gap, the authors reconnoitre the positive potential areas concentrating on the use and applications of social media by pharmacist to improvise & reinvigorate determinants of health outcomes. The subject was extensively studied in five main modules. 1. Encapsulating the complete medical information 2. Innocuous usage of medicine 3. In perpetuity medication usage 4. Document based medicine & implementation guidelines & 5. Clinical inquisition. In each of t...