Sapphire Doan | De Anza College (original) (raw)
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Universidad Autónoma Metropolitana-Cuajimalpa
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Papers by Sapphire Doan
bioRxiv (Cold Spring Harbor Laboratory), Mar 6, 2023
ABSTRACTNeuronal tracing methods are essential tools to understand the fundamental architecture o... more ABSTRACTNeuronal tracing methods are essential tools to understand the fundamental architecture of neural circuits and their connection to the overall functional behavior of the brain. Viral vectors used to map these transsynaptic connections are capable of cell-type-specific and directional-specific labeling of the neuronal connections. Herein, we describe a novel approach to guide the transsynaptic spreading of the Rabies Virus (RV) retrograde tracer using light. We built a Baculovirus (BV) as a helper virus to deliver all the functional components necessary and sufficient for a nontoxic RV to spread from neuron to neuron, with a light-actuated gene switch to control the RV polymerase, theL gene. This design should allow for precisely controlled polysynaptic viral tracing with minimal viral toxicity. To use this system in a highly scalable and automated manner, we built optoelectronics for controlling this systemin vitrowith a large field of view using an off-the-shelf CMOS sensor...
bioRxiv (Cold Spring Harbor Laboratory), Mar 6, 2023
ABSTRACTNeuronal tracing methods are essential tools to understand the fundamental architecture o... more ABSTRACTNeuronal tracing methods are essential tools to understand the fundamental architecture of neural circuits and their connection to the overall functional behavior of the brain. Viral vectors used to map these transsynaptic connections are capable of cell-type-specific and directional-specific labeling of the neuronal connections. Herein, we describe a novel approach to guide the transsynaptic spreading of the Rabies Virus (RV) retrograde tracer using light. We built a Baculovirus (BV) as a helper virus to deliver all the functional components necessary and sufficient for a nontoxic RV to spread from neuron to neuron, with a light-actuated gene switch to control the RV polymerase, theL gene. This design should allow for precisely controlled polysynaptic viral tracing with minimal viral toxicity. To use this system in a highly scalable and automated manner, we built optoelectronics for controlling this systemin vitrowith a large field of view using an off-the-shelf CMOS sensor...