Pushpendra Jain | Dr. H.S. Gour Central University, Sagar, MP (original) (raw)

Papers by Pushpendra Jain

Emblica Officinalis (Amla, EO and E. officinalis) is one of the most important herbs in the India... more Emblica Officinalis (Amla, EO and E. officinalis) is one of the most important herbs in the Indian traditional medicine system, especially Ayurveda and also known as King of all medicinal plants. Emblica officinalis is famous Ayurvedic herb (the name means sour in Sanskrit) is one of the most useful medicines in the Indian pharmacopoeia, and is considered to be one of the strongest uvenatives (Rasayana), particularly for the blood, bones, liver and heart. It is an exceptionally rich source of vitamin C containing thirty times the amount found in oranges. It is one of the oldest oriental medicines mentioned in Ayurveda as potential remedy for various ailments. Emblica officinalis (Amla) is widely used in the ayurvedic medicines and believed to increase defense /immune power against diseases. Several parts of the plant are used to treat a variety of diseases, but the most important is the “fruit”. The fruit is rich in quercetin, phyllaemblic compounds, gallic acid, tannins, flavonoids...

International Journal of Pharmacy and Pharmaceutical Sciences, Apr 1, 2015

Objective: To evaluate the analgesic activity of aqueous extract of rhizomes of Acorus calamusin ... more Objective: To evaluate the analgesic activity of aqueous extract of rhizomes of Acorus calamusin mice. Methods: Analgesic activity of aqueous extracts of A. calamus at different doses were studied using the hot plate method. The study was carried out at three different dose levels (5, 25 and 50 mg/kg) of A. calamus, given subcutaneously to mice. Results: The different dose of aqueous extracts of A. calamus showed dose and time dependent analgesic effect and maximum effect was observed at 50 mg/kg dose of extract after 90 min of administration, which was comparable to diclofenac sodium, used as standard drug in the study. Conclusion: The aqueous extract of rhizomes of A. calamus was found to be effective analgesic in mice using the hot plate method. This study merits further detailed pharmacological investigation of this extract as well as isolation and characterization of its bioactive chemical constituents (s).

In the last few decades, a curious thing has happened to Herbal medicines. Instead of being kille... more In the last few decades, a curious thing has happened to Herbal medicines. Instead of being killed off by medical science and pharmaceutical chemistry, it has made come back. Herbal medicine has benefited from the objective analysis of the medical science, while fanciful and emotional claims for herbal cures have been thrown out, herbal treatments and plant medicine that works have been acknowledge. And herbal medicine has been found to have impressive credentials. Developed empirically by trial and error, many herbal treatments were nevertheless remarkably effective. In a recent survey estimated that 39% of all 520 new approved drugs in 1983-1994 were natural products or derived from natural products and 60-80% of antibacterial and anticancer drugs were derived from natural products. Medicinal plants play an important role in the development of potent therapeutic agents. Plant derived drugs came into use in the modern medicine through the uses of plant material as indigenous cure i...

International journal of health sciences

Aim: To formulate and evaluate of transdermal patches of ciprofloxacin hydrochloride. Materials a... more Aim: To formulate and evaluate of transdermal patches of ciprofloxacin hydrochloride. Materials and Methods: To maximise bioavailability by avoiding pre-systemic hepatic metabolism, an effort was made to design a transdermal treatment system for Ciprofloxacin hydrochloride employing polymers such as HPMC, EC, and ERS100. Physical and chemical characteristics, in vitro drug release investigations and stability experiments were carried out on the prepared patches. Results: T1, T8, and T15 formulations were chosen for stability experiments at room temperature and 40 °C for a period of 45 days, respectively. The findings of the stability experiments indicated that the prepared patches may be stored for up to 45 days. The T8 formulation has shown promising results, thus the correct method should be used for commercial and scale manufacturing. Conclusion: The effectiveness of these patches will need to be studied over the course of many years in pharmacokinetic and pharmacodynamic investi...

International journal of health sciences

Aim: To minimise toxicity and alter pharmacokinetic and bioavailability, niosomes are becoming mo... more Aim: To minimise toxicity and alter pharmacokinetic and bioavailability, niosomes are becoming more significant in medication delivery. Rheumatoid arthritis, juvenile rheumatoid arthritis, and degenerative joint disease may benefit from this new non-steroidal anti-inflammatory, antipyretic, and analgesic drug. Materials and Methods: Non-ionic surfactants (Span 40, 60, and Tween 60) and cholesterol were used in various ratios to create tolmetin sodium niosomes in a thin film hydration process (CHO). Evaluations of the formulations were conducted in terms of size, shape, encapsulation efficiency, and in vitro drug release. Results: In vitro drug release ranged from 94.87±0.45 to 93.19±0.45 percent in 24 hours for niosomes that looked spherical in entrapment efficiency. Incorporating Tolmetin sodium niosomes with Span 60 and CHO in the ratio of 1:2.1 into Carbopol gel was shown to be promising. Tolmetin drug noisome has a drug content and pH of 99.19±1.20 and 7.0±0.06, respectively. Co...

Journal of chemical and pharmaceutical research, 2016

In present study, an attempt has been made to evaluate a Comparative study of powder v/s gel of p... more In present study, an attempt has been made to evaluate a Comparative study of powder v/s gel of proniosomal drug delivery system of flurbiprofen. Flurbiprofen, are nonsteroidal anti-inflammatory drug (NSAIDs) is used for the relief of pain and inflammation associated with rheumatoid arthritis and osteoarthritis. It exhibits antiinflammatory, analgesic and antipyretic activities. It will be also effected the transdermal system rate because of its size, nature and chemistry, these systems give better drug permeability from biological bioavailability membranes and helps in solubilization of some practically insoluble drugs and hence solve problems of many drug. In the present study we are using various surfactants like span 20, 40, 60, 80, and brij 35 and studided in various proniosomal powder and gel formulation and studied in both result in following formulation. In this result proniosomal gel of formulation brij 35 show better entrapment and highest in vitro drug release. The best r...

International Journal of Pharmacy and Pharmaceutical Sciences, 2015

Objective: The literature survey revealed that various methods are reported for determination of ... more Objective: The literature survey revealed that various methods are reported for determination of Irbesartan and Hydrochlorothiazide alone or in combination with other drugs So, the main objective of our study was to develop simple, accurate and reproducible methods for the simultaneous estimation of Irbesartan and Hydrochlorothiazide in a combined dosage form. Methods: The method for the simultaneous determination of Irbesartan and Hydrochlorothiazide by spectroscopy has been developed. The simple, accurate and precise method includes Area Under the Curve (AUC) method. On response to the effect of solvent on spectral behaviors of Irbesartan and Hydrochlorothiazide, methanol was selected as solvent. Irbesartan shows maximum absorbance at 224 nm and Hydrochlorothiazide shows maximum absorbance at 271 nm. For the AUC method, the wavelength ranges between 225-230 nm and 258-265 nm respectively were selected with reference to the absorbance curves plotted between the wavelengths of 200-4...

It is said that the use of Bacopa monnieri for memory enhancement goes back 3000 years or more in... more It is said that the use of Bacopa monnieri for memory enhancement goes back 3000 years or more in India, when it was cited for its medicinal properties, especially the memory enhancing capacity, in the Vedic texts "Athar-Ved Samhila" (3:1) of 800 B.C. and in Ayurveda. In the folklore of Indian medicine, several herbs have been used traditionally as brain or nerve tonics. One of the most popular of these neurotonics is Bacopa monnieri , a well known memory booster. Brahmi has been administered at religious institutions to help students to enhance their memory for learning ancient, religious hymns. It is also used as cardio-tonic, tranquilizer and sedative, improves process of learning, Restores memory, enhances power of speech and imagination, diuretic and nervine tonic, anti stress, for nervous and mental strain, use in insanity, epilepsy, hysteria, aesthenia, nervous breakdown. It is a small, creeping succulent herb. The leaf and flower bearing stems are 10- 30 cm long an...

INDIAN DRUGS, 2020

This study was undertaken to investigate the in vivo wound healing activity of ethanol extracts o... more This study was undertaken to investigate the in vivo wound healing activity of ethanol extracts of the aerial parts of Euphorbia hirta Linn. and its polyherbal formulation with Tridax procumbens and Eclipta alba. The in vivo wound healing activity was assessed in albino rats by using three wound models i.e. excision wound, incision wound and dead space wound models. Level of biochemical markers and histological changes were monitored to evaluate the enhancement of wound healing. The results showed that ethanolic extract ointment possesses a definite prohealing action. This was demonstrated by a significant increase in the rate of wound contraction and by enhanced epithelization. Significant increase in tensile strength, hydroxyproline content and collagen levels were observed, which was further supported by histopathological studies and gain in granuloma breaking strength.

International Journal of Pharmacy and Pharmaceutical Sciences, Feb 24, 2015

Journal of Liposome Research, 2013

Andrographis paniculata is a medicinal herb used extensively for various ailments and contains th... more Andrographis paniculata is a medicinal herb used extensively for various ailments and contains therapeutically active phytoconstituent, andrographolide (AN). Although hepatoprotective activity of AN is established, but their bioavailability is restricted due to its rapid clearance. The aim of this study, therefore, was to formulate AN herbosomes (ANH) through complexation with naturally occurring soya-phosphatidylcholine (SPC), in order to enhance absorption. Prepared andrographolide-soy phosphatidylcholine (AN-SPC) complex prepared was subjected for characterisation of complex and formation of vesicular system known as ANH using rotary evaporation techniques. This complex was subjected to in vitro study using everted small intestine sac technique which showed significantly increased absorption of AN from the ANH as compared to the plain AN. The hepatoprotective potential of ANH and plain AN was evaluated using carbon tetrachloride inducing hepatotoxicity rat model and compared, in which ANH equivalent to 50 mg/kg of plain AN significantly restore serum glutamate oxalacetate transaminase (112.4 ± 9.67 for AN whereas 90.2 ± 4.23 for ANH) and serum glutamate pyruvate transaminase (109.3 ± 7.89 for AN whereas 90.6 ± 4.34 for ANH) level as compared to control group. The ANH showed significantly better absorption than plain AN and this effect of ANH was also comparable to the standard drug (Silymarin). The findings of present study reveal that ANH has better bioavailability as shown by in vitro absorption study and hence improved hepatoprotection as compared to plain AN at equivalent dose.

Drug Development and Industrial Pharmacy, 2012

Fatty liver is the first stage of alcoholic damage which is reversible with abstinence from alcoh... more Fatty liver is the first stage of alcoholic damage which is reversible with abstinence from alcohol. Mangiferin (MF) showed potent scavenging activity on diphenyl-1-picrylhydrazyl radicals which stimulate liver regeneration in various liver injuries. Although, MF shows hepatoprotection against various liver disorders but due to rapid clearance and limited solubility in lipoid environment, there is problem of its poor absorption from intestine hence poor bioavailability. Owing to which there is a need to develop MF herbosomes to resolve the problem of poor bioavailability to enhance the therapeutic potential. Successfully prepared MF herbosomes through complexation with phospholipids were characterized by physicochemical, chromatography, spectroscopy (differential scanning calorimetry (DSC), infrared (IR), and nuclear magnetic resonance (NMR)), ex vivo absorption using everted small intestine sac technique and in vivo studies using ethanol inducing hepatotoxicity in albino rats and comparing the results against plain MF. Ex vivo study showed significant increased absorption of MF from prepared MF herbosomes as compared to plain MF. The hepatoprotective potential of MF herbosomes evaluated by in vivo study revealed significantly decreased levels of serum glutamate oxaloacetate transminase (SGOT), serum glutamate pyruvate transminase (SGPT), total bilirubin, and alkaline phosphatase (ALP) in MF herbosomes as compared to plain MF. MF herbosomes also showed significantly decreased level of malonyl dehydrogenase along with increased levels of reduced glutathione, superoxide dismutase (SOD) and catalase as compared to plain MF which was also comparable to the standard drug, silymarin (SL). The above mentioned results showed that hepatoprotective and antioxidant potency of MF enhanced due to the preparation of its herbosomes.

Present study deals with the development and characterization of aloe vera cosmetic herbal hydrog... more Present study deals with the development and characterization of aloe vera cosmetic herbal hydrogel preparation using inner part of aloe vera leaf, acacia, hydroxy propyl methyl cellulose (HPMC), carbopol 934, glycerine, tartaric acid, potassium sorbate and sodium benzoate. Aloe vera liquid was prepared by heating at low temperature and the hydrogel was prepared by simple dissolving method of other ingredients in a specific manner. Four formulations were developed which differ in the ratio of hydrogel forming polymers. Formulation A1, A2, A3 and A4 were composed of acacia, HPMC, carbopol 934 in the ratio of 1:1:1, 1:2:1, 2:1:1 and 1:1:2 respectively. All the formulations were evaluated for percentage moisture content, transparency, smoothness, weight on drying, viscosity, pH and microbial growth. On the basis of evaluation parameter formulation A4 was selected as developed formulation. On the basis of evaluation study, it can be concluded that aloe vera cosmetic herbal hydrogel may ...

International Journal of Pharmacology and Pharmaceutical Technology

Plant products play a crucial role in the hepatoprotection through its antioxidants property. The... more Plant products play a crucial role in the hepatoprotection through its antioxidants property. Therefore, search for modern medicine of plant origin with this property has become a central focus on hepatoprotection today. This study investigated to search a new hepatoprotective agent from natural sources, the methanol extract of nutitional plant, Daucus carota leaves was tested against liver damage of albino rats. Levels of serum marker enzymes i.e. SGOT, SGPT (aminotransferases), ALP (alkaline phosphatase) and TB (total bilirubin) in serum respectively. The histopathological changes of liver sections were also compared with the respective controls. 30% paracetamol induced significant (P<0.05) increase in liver enzymes alongwith the hepatic necrosis and other visible disarrangements in hepatic tissues. Simultaneously, oral treatment with kaempferol (KF) isolated from D. carota plant reversed to all the serum and liver parameters, dose-dependently, in 30% paracetamol treated rats. ...

Advance Pharmaceutical Journal

Tylophora indica (Burm f.) Merill. (Family: Asclepidaceae) generally popular as Antmul is a twini... more Tylophora indica (Burm f.) Merill. (Family: Asclepidaceae) generally popular as Antmul is a twining perennial plant seen in southern and eastern region of India in planes, forests and hilly areas. This is seen growing in many states in India like Bengal, Uttar Pradesh, Assam, Orissa (Wealth of India, 1969-1972). The plant is a climber or shrub with branches and is observed growing up to height of 1.5 meters, Leaves shape are ovateoblong to elliptic-oblong with 3-10 cm length and 1.5-7 cm width (Kirtikar and Basu, 1991). Plant roots are long fleshy and fissured along the length and are with light brown, corky bark. The plant has little size flowers and are 1-1.5 cm across, in 2-3 flowers fascicles (bundle) in axillary umbellate cymes. Calyx has divisions almost to the base, looks massively hairy from outside; segments laneolate, acute. Corolla is green to yellow or geen to purple, lobes oblong and acute. Fruit a follicle, up to 7 X 1 cm, ovoid lanceolate, tapering at top forming fine mucro, finally striate, glabrous, seeds 0.6-0.8 X 0.3-0.4 cm long. The plant is originated from India. The plant can be found at an elevation of 1260 m in the sub Himalayan tract, central, peninsular India. It is seen in Eastern, North-ast and Bengal and parts of South India. Tylophora indica is a perennial plant with long thick roots branching climber .The plant is observed growing in planes and hilly land in India upto an altitude of 1000 meter in various states like Assam, Bengal, Orissa (Gupta, 2003). Phytochemistry The plant contain various active ingredients like tylophorine,tylophorinine, tylophorinidine and in plant body,leaves roots (Mohammad, 2008) Roots and leaves of plant also contain septidine too. The plant body also contain alkaloids were reported desmethyltylophorine, methyltylophorinindine, isotylocrebrine, skimmianine, gamafagarine. The compounds which are non alkaloidal and isolated are quercetin, kaempferol, octacosanoate, sigmasterol, betasitosterol, tyloindane, cetylalcohol, wax, resin, coutchone, pigments, tannins, glucose, calcium salts, potassium chloride (Gupta et al., 2010).

Andrographis paniculata is a medicinal herb used extensively for various ailments and contains th... more Andrographis paniculata is a medicinal herb used extensively for various ailments and contains therapeutically active phytoconstituent, andrographolide (AN). Although hepatoprotective activity of AN is established, but their bioavailability is restricted due to its rapid clearance. The aim of this study, therefore, was to formulate AN herbosomes (ANH) through complexation with naturally occurring soya-phosphatidylcholine (SPC), in order to enhance absorption. Prepared andrographolide-soy phosphatidylcholine (AN-SPC) complex prepared was subjected for characterisation of complex and formation of vesicular system known as ANH using rotary evaporation techniques. This complex was subjected to in vitro study using everted small intestine sac technique which showed significantly increased absorption of AN from the ANH as compared to the plain AN. The hepatoprotective potential of ANH and plain AN was evaluated using carbon tetrachloride inducing hepatotoxicity rat model and compared, in which ANH equivalent to 50 mg/kg of plain AN significantly restore serum glutamate oxalacetate transaminase (112.4 AE 9.67 for AN whereas 90.2 AE 4.23 for ANH) and serum glutamate pyruvate transaminase (109.3 AE 7.89 for AN whereas 90.6 AE 4.34 for ANH) level as compared to control group. The ANH showed significantly better absorption than plain AN and this effect of ANH was also comparable to the standard drug (Silymarin). The findings of present study reveal that ANH has better bioavailability as shown by in vitro absorption study and hence improved hepatoprotection as compared to plain AN at equivalent dose.

Emblica Officinalis (Amla, EO and E. officinalis) is one of the most important herbs in the India... more Emblica Officinalis (Amla, EO and E. officinalis) is one of the most important herbs in the Indian traditional medicine system, especially Ayurveda and also known as King of all medicinal plants. Emblica officinalis is famous Ayurvedic herb (the name means sour in Sanskrit) is one of the most useful medicines in the Indian pharmacopoeia, and is considered to be one of the strongest uvenatives (Rasayana), particularly for the blood, bones, liver and heart. It is an exceptionally rich source of vitamin C containing thirty times the amount found in oranges. It is one of the oldest oriental medicines mentioned in Ayurveda as potential remedy for various ailments. Emblica officinalis (Amla) is widely used in the ayurvedic medicines and believed to increase defense /immune power against diseases. Several parts of the plant are used to treat a variety of diseases, but the most important is the “fruit”. The fruit is rich in quercetin, phyllaemblic compounds, gallic acid, tannins, flavonoids...

International Journal of Pharmacy and Pharmaceutical Sciences, Apr 1, 2015

Objective: To evaluate the analgesic activity of aqueous extract of rhizomes of Acorus calamusin ... more Objective: To evaluate the analgesic activity of aqueous extract of rhizomes of Acorus calamusin mice. Methods: Analgesic activity of aqueous extracts of A. calamus at different doses were studied using the hot plate method. The study was carried out at three different dose levels (5, 25 and 50 mg/kg) of A. calamus, given subcutaneously to mice. Results: The different dose of aqueous extracts of A. calamus showed dose and time dependent analgesic effect and maximum effect was observed at 50 mg/kg dose of extract after 90 min of administration, which was comparable to diclofenac sodium, used as standard drug in the study. Conclusion: The aqueous extract of rhizomes of A. calamus was found to be effective analgesic in mice using the hot plate method. This study merits further detailed pharmacological investigation of this extract as well as isolation and characterization of its bioactive chemical constituents (s).

In the last few decades, a curious thing has happened to Herbal medicines. Instead of being kille... more In the last few decades, a curious thing has happened to Herbal medicines. Instead of being killed off by medical science and pharmaceutical chemistry, it has made come back. Herbal medicine has benefited from the objective analysis of the medical science, while fanciful and emotional claims for herbal cures have been thrown out, herbal treatments and plant medicine that works have been acknowledge. And herbal medicine has been found to have impressive credentials. Developed empirically by trial and error, many herbal treatments were nevertheless remarkably effective. In a recent survey estimated that 39% of all 520 new approved drugs in 1983-1994 were natural products or derived from natural products and 60-80% of antibacterial and anticancer drugs were derived from natural products. Medicinal plants play an important role in the development of potent therapeutic agents. Plant derived drugs came into use in the modern medicine through the uses of plant material as indigenous cure i...

International journal of health sciences

Aim: To formulate and evaluate of transdermal patches of ciprofloxacin hydrochloride. Materials a... more Aim: To formulate and evaluate of transdermal patches of ciprofloxacin hydrochloride. Materials and Methods: To maximise bioavailability by avoiding pre-systemic hepatic metabolism, an effort was made to design a transdermal treatment system for Ciprofloxacin hydrochloride employing polymers such as HPMC, EC, and ERS100. Physical and chemical characteristics, in vitro drug release investigations and stability experiments were carried out on the prepared patches. Results: T1, T8, and T15 formulations were chosen for stability experiments at room temperature and 40 °C for a period of 45 days, respectively. The findings of the stability experiments indicated that the prepared patches may be stored for up to 45 days. The T8 formulation has shown promising results, thus the correct method should be used for commercial and scale manufacturing. Conclusion: The effectiveness of these patches will need to be studied over the course of many years in pharmacokinetic and pharmacodynamic investi...

International journal of health sciences

Aim: To minimise toxicity and alter pharmacokinetic and bioavailability, niosomes are becoming mo... more Aim: To minimise toxicity and alter pharmacokinetic and bioavailability, niosomes are becoming more significant in medication delivery. Rheumatoid arthritis, juvenile rheumatoid arthritis, and degenerative joint disease may benefit from this new non-steroidal anti-inflammatory, antipyretic, and analgesic drug. Materials and Methods: Non-ionic surfactants (Span 40, 60, and Tween 60) and cholesterol were used in various ratios to create tolmetin sodium niosomes in a thin film hydration process (CHO). Evaluations of the formulations were conducted in terms of size, shape, encapsulation efficiency, and in vitro drug release. Results: In vitro drug release ranged from 94.87±0.45 to 93.19±0.45 percent in 24 hours for niosomes that looked spherical in entrapment efficiency. Incorporating Tolmetin sodium niosomes with Span 60 and CHO in the ratio of 1:2.1 into Carbopol gel was shown to be promising. Tolmetin drug noisome has a drug content and pH of 99.19±1.20 and 7.0±0.06, respectively. Co...

Journal of chemical and pharmaceutical research, 2016

In present study, an attempt has been made to evaluate a Comparative study of powder v/s gel of p... more In present study, an attempt has been made to evaluate a Comparative study of powder v/s gel of proniosomal drug delivery system of flurbiprofen. Flurbiprofen, are nonsteroidal anti-inflammatory drug (NSAIDs) is used for the relief of pain and inflammation associated with rheumatoid arthritis and osteoarthritis. It exhibits antiinflammatory, analgesic and antipyretic activities. It will be also effected the transdermal system rate because of its size, nature and chemistry, these systems give better drug permeability from biological bioavailability membranes and helps in solubilization of some practically insoluble drugs and hence solve problems of many drug. In the present study we are using various surfactants like span 20, 40, 60, 80, and brij 35 and studided in various proniosomal powder and gel formulation and studied in both result in following formulation. In this result proniosomal gel of formulation brij 35 show better entrapment and highest in vitro drug release. The best r...

International Journal of Pharmacy and Pharmaceutical Sciences, 2015

Objective: The literature survey revealed that various methods are reported for determination of ... more Objective: The literature survey revealed that various methods are reported for determination of Irbesartan and Hydrochlorothiazide alone or in combination with other drugs So, the main objective of our study was to develop simple, accurate and reproducible methods for the simultaneous estimation of Irbesartan and Hydrochlorothiazide in a combined dosage form. Methods: The method for the simultaneous determination of Irbesartan and Hydrochlorothiazide by spectroscopy has been developed. The simple, accurate and precise method includes Area Under the Curve (AUC) method. On response to the effect of solvent on spectral behaviors of Irbesartan and Hydrochlorothiazide, methanol was selected as solvent. Irbesartan shows maximum absorbance at 224 nm and Hydrochlorothiazide shows maximum absorbance at 271 nm. For the AUC method, the wavelength ranges between 225-230 nm and 258-265 nm respectively were selected with reference to the absorbance curves plotted between the wavelengths of 200-4...

It is said that the use of Bacopa monnieri for memory enhancement goes back 3000 years or more in... more It is said that the use of Bacopa monnieri for memory enhancement goes back 3000 years or more in India, when it was cited for its medicinal properties, especially the memory enhancing capacity, in the Vedic texts "Athar-Ved Samhila" (3:1) of 800 B.C. and in Ayurveda. In the folklore of Indian medicine, several herbs have been used traditionally as brain or nerve tonics. One of the most popular of these neurotonics is Bacopa monnieri , a well known memory booster. Brahmi has been administered at religious institutions to help students to enhance their memory for learning ancient, religious hymns. It is also used as cardio-tonic, tranquilizer and sedative, improves process of learning, Restores memory, enhances power of speech and imagination, diuretic and nervine tonic, anti stress, for nervous and mental strain, use in insanity, epilepsy, hysteria, aesthenia, nervous breakdown. It is a small, creeping succulent herb. The leaf and flower bearing stems are 10- 30 cm long an...

INDIAN DRUGS, 2020

This study was undertaken to investigate the in vivo wound healing activity of ethanol extracts o... more This study was undertaken to investigate the in vivo wound healing activity of ethanol extracts of the aerial parts of Euphorbia hirta Linn. and its polyherbal formulation with Tridax procumbens and Eclipta alba. The in vivo wound healing activity was assessed in albino rats by using three wound models i.e. excision wound, incision wound and dead space wound models. Level of biochemical markers and histological changes were monitored to evaluate the enhancement of wound healing. The results showed that ethanolic extract ointment possesses a definite prohealing action. This was demonstrated by a significant increase in the rate of wound contraction and by enhanced epithelization. Significant increase in tensile strength, hydroxyproline content and collagen levels were observed, which was further supported by histopathological studies and gain in granuloma breaking strength.

International Journal of Pharmacy and Pharmaceutical Sciences, Feb 24, 2015

Journal of Liposome Research, 2013

Andrographis paniculata is a medicinal herb used extensively for various ailments and contains th... more Andrographis paniculata is a medicinal herb used extensively for various ailments and contains therapeutically active phytoconstituent, andrographolide (AN). Although hepatoprotective activity of AN is established, but their bioavailability is restricted due to its rapid clearance. The aim of this study, therefore, was to formulate AN herbosomes (ANH) through complexation with naturally occurring soya-phosphatidylcholine (SPC), in order to enhance absorption. Prepared andrographolide-soy phosphatidylcholine (AN-SPC) complex prepared was subjected for characterisation of complex and formation of vesicular system known as ANH using rotary evaporation techniques. This complex was subjected to in vitro study using everted small intestine sac technique which showed significantly increased absorption of AN from the ANH as compared to the plain AN. The hepatoprotective potential of ANH and plain AN was evaluated using carbon tetrachloride inducing hepatotoxicity rat model and compared, in which ANH equivalent to 50 mg/kg of plain AN significantly restore serum glutamate oxalacetate transaminase (112.4 ± 9.67 for AN whereas 90.2 ± 4.23 for ANH) and serum glutamate pyruvate transaminase (109.3 ± 7.89 for AN whereas 90.6 ± 4.34 for ANH) level as compared to control group. The ANH showed significantly better absorption than plain AN and this effect of ANH was also comparable to the standard drug (Silymarin). The findings of present study reveal that ANH has better bioavailability as shown by in vitro absorption study and hence improved hepatoprotection as compared to plain AN at equivalent dose.

Drug Development and Industrial Pharmacy, 2012

Fatty liver is the first stage of alcoholic damage which is reversible with abstinence from alcoh... more Fatty liver is the first stage of alcoholic damage which is reversible with abstinence from alcohol. Mangiferin (MF) showed potent scavenging activity on diphenyl-1-picrylhydrazyl radicals which stimulate liver regeneration in various liver injuries. Although, MF shows hepatoprotection against various liver disorders but due to rapid clearance and limited solubility in lipoid environment, there is problem of its poor absorption from intestine hence poor bioavailability. Owing to which there is a need to develop MF herbosomes to resolve the problem of poor bioavailability to enhance the therapeutic potential. Successfully prepared MF herbosomes through complexation with phospholipids were characterized by physicochemical, chromatography, spectroscopy (differential scanning calorimetry (DSC), infrared (IR), and nuclear magnetic resonance (NMR)), ex vivo absorption using everted small intestine sac technique and in vivo studies using ethanol inducing hepatotoxicity in albino rats and comparing the results against plain MF. Ex vivo study showed significant increased absorption of MF from prepared MF herbosomes as compared to plain MF. The hepatoprotective potential of MF herbosomes evaluated by in vivo study revealed significantly decreased levels of serum glutamate oxaloacetate transminase (SGOT), serum glutamate pyruvate transminase (SGPT), total bilirubin, and alkaline phosphatase (ALP) in MF herbosomes as compared to plain MF. MF herbosomes also showed significantly decreased level of malonyl dehydrogenase along with increased levels of reduced glutathione, superoxide dismutase (SOD) and catalase as compared to plain MF which was also comparable to the standard drug, silymarin (SL). The above mentioned results showed that hepatoprotective and antioxidant potency of MF enhanced due to the preparation of its herbosomes.

Present study deals with the development and characterization of aloe vera cosmetic herbal hydrog... more Present study deals with the development and characterization of aloe vera cosmetic herbal hydrogel preparation using inner part of aloe vera leaf, acacia, hydroxy propyl methyl cellulose (HPMC), carbopol 934, glycerine, tartaric acid, potassium sorbate and sodium benzoate. Aloe vera liquid was prepared by heating at low temperature and the hydrogel was prepared by simple dissolving method of other ingredients in a specific manner. Four formulations were developed which differ in the ratio of hydrogel forming polymers. Formulation A1, A2, A3 and A4 were composed of acacia, HPMC, carbopol 934 in the ratio of 1:1:1, 1:2:1, 2:1:1 and 1:1:2 respectively. All the formulations were evaluated for percentage moisture content, transparency, smoothness, weight on drying, viscosity, pH and microbial growth. On the basis of evaluation parameter formulation A4 was selected as developed formulation. On the basis of evaluation study, it can be concluded that aloe vera cosmetic herbal hydrogel may ...

International Journal of Pharmacology and Pharmaceutical Technology

Plant products play a crucial role in the hepatoprotection through its antioxidants property. The... more Plant products play a crucial role in the hepatoprotection through its antioxidants property. Therefore, search for modern medicine of plant origin with this property has become a central focus on hepatoprotection today. This study investigated to search a new hepatoprotective agent from natural sources, the methanol extract of nutitional plant, Daucus carota leaves was tested against liver damage of albino rats. Levels of serum marker enzymes i.e. SGOT, SGPT (aminotransferases), ALP (alkaline phosphatase) and TB (total bilirubin) in serum respectively. The histopathological changes of liver sections were also compared with the respective controls. 30% paracetamol induced significant (P<0.05) increase in liver enzymes alongwith the hepatic necrosis and other visible disarrangements in hepatic tissues. Simultaneously, oral treatment with kaempferol (KF) isolated from D. carota plant reversed to all the serum and liver parameters, dose-dependently, in 30% paracetamol treated rats. ...

Advance Pharmaceutical Journal

Tylophora indica (Burm f.) Merill. (Family: Asclepidaceae) generally popular as Antmul is a twini... more Tylophora indica (Burm f.) Merill. (Family: Asclepidaceae) generally popular as Antmul is a twining perennial plant seen in southern and eastern region of India in planes, forests and hilly areas. This is seen growing in many states in India like Bengal, Uttar Pradesh, Assam, Orissa (Wealth of India, 1969-1972). The plant is a climber or shrub with branches and is observed growing up to height of 1.5 meters, Leaves shape are ovateoblong to elliptic-oblong with 3-10 cm length and 1.5-7 cm width (Kirtikar and Basu, 1991). Plant roots are long fleshy and fissured along the length and are with light brown, corky bark. The plant has little size flowers and are 1-1.5 cm across, in 2-3 flowers fascicles (bundle) in axillary umbellate cymes. Calyx has divisions almost to the base, looks massively hairy from outside; segments laneolate, acute. Corolla is green to yellow or geen to purple, lobes oblong and acute. Fruit a follicle, up to 7 X 1 cm, ovoid lanceolate, tapering at top forming fine mucro, finally striate, glabrous, seeds 0.6-0.8 X 0.3-0.4 cm long. The plant is originated from India. The plant can be found at an elevation of 1260 m in the sub Himalayan tract, central, peninsular India. It is seen in Eastern, North-ast and Bengal and parts of South India. Tylophora indica is a perennial plant with long thick roots branching climber .The plant is observed growing in planes and hilly land in India upto an altitude of 1000 meter in various states like Assam, Bengal, Orissa (Gupta, 2003). Phytochemistry The plant contain various active ingredients like tylophorine,tylophorinine, tylophorinidine and in plant body,leaves roots (Mohammad, 2008) Roots and leaves of plant also contain septidine too. The plant body also contain alkaloids were reported desmethyltylophorine, methyltylophorinindine, isotylocrebrine, skimmianine, gamafagarine. The compounds which are non alkaloidal and isolated are quercetin, kaempferol, octacosanoate, sigmasterol, betasitosterol, tyloindane, cetylalcohol, wax, resin, coutchone, pigments, tannins, glucose, calcium salts, potassium chloride (Gupta et al., 2010).

Andrographis paniculata is a medicinal herb used extensively for various ailments and contains th... more Andrographis paniculata is a medicinal herb used extensively for various ailments and contains therapeutically active phytoconstituent, andrographolide (AN). Although hepatoprotective activity of AN is established, but their bioavailability is restricted due to its rapid clearance. The aim of this study, therefore, was to formulate AN herbosomes (ANH) through complexation with naturally occurring soya-phosphatidylcholine (SPC), in order to enhance absorption. Prepared andrographolide-soy phosphatidylcholine (AN-SPC) complex prepared was subjected for characterisation of complex and formation of vesicular system known as ANH using rotary evaporation techniques. This complex was subjected to in vitro study using everted small intestine sac technique which showed significantly increased absorption of AN from the ANH as compared to the plain AN. The hepatoprotective potential of ANH and plain AN was evaluated using carbon tetrachloride inducing hepatotoxicity rat model and compared, in which ANH equivalent to 50 mg/kg of plain AN significantly restore serum glutamate oxalacetate transaminase (112.4 AE 9.67 for AN whereas 90.2 AE 4.23 for ANH) and serum glutamate pyruvate transaminase (109.3 AE 7.89 for AN whereas 90.6 AE 4.34 for ANH) level as compared to control group. The ANH showed significantly better absorption than plain AN and this effect of ANH was also comparable to the standard drug (Silymarin). The findings of present study reveal that ANH has better bioavailability as shown by in vitro absorption study and hence improved hepatoprotection as compared to plain AN at equivalent dose.