Dhara Dixit | Kskv Kachchh University (original) (raw)

Papers by Dhara Dixit

Research paper thumbnail of A sustainable synthesis of a CuO@C nanocomposite for the remediation of organic dyes in water and its antibacterial properties

Nano-structures & nano-objects, May 1, 2024

Research paper thumbnail of Omics Approaches for Algal Applications

Springer eBooks, Nov 30, 2023

Research paper thumbnail of Evaluation of Tropical Edible Seaweeds Across the Untapped Frontier of Gok for Boosting the Expanse’s Fiscal Valorization

Proceedings of the National Academy of Sciences, India Section B: Biological Sciences

Research paper thumbnail of Untargeted secondary metabolite profiling and putative pathway deduction of a marine Ochrophyte – Iyengaria stellata (Børgesen) using liquid chromatography-mass spectrometry

Research paper thumbnail of Recent Advances in Biotechnology of Seaweeds: An Overview

Sustainable Global Resources Of Seaweeds Volume 1, 2022

Research paper thumbnail of Synthesis, characterization and application of green seaweed mediated silver nanoparticles (AgNPs) as antibacterial agents for water disinfection

A simple and eco-friendly method for the synthesis of hybrid bead silver nanoparticles (AgNPs) em... more A simple and eco-friendly method for the synthesis of hybrid bead silver nanoparticles (AgNPs) employing the aqueous extract derived from natural and renewable source namely tropical benthic green seaweed Ulva flexuosa was developed. This route involves the reduction of Ag+ ions anchored onto macro porous methacrylic acid copolymer beads to AgNPs for employing them as antibacterial agents for in vitro water disinfection. The seaweed extract itself acts as a reducing and stabilizing agent and requires no additional surfactant or capping agent for forming the AgNPs. The nanoparticles were analyzed using high-resolution transmission electron microscopy, UV-Vis spectroscopy, Fourier transform infrared spectroscopy, scanning electron microscopy, energy dispersive X-ray analysis and inductively coupled plasma optical emission spectroscopy. The study elucidates that such biologically synthesized AgNPs exhibit potential antibacterial activity against two Gram positive (Bacillus subtilis, St...

Research paper thumbnail of Non‐targeted metabolomics approach to assess the brown marine macroalga Dictyota dichotoma as a functional food using liquid chromatography with mass spectrometry

SEPARATION SCIENCE PLUS, 2020

Research paper thumbnail of Assessment of the Nutritive, Biochemical, Antioxidant and Antibacterial Potential of Eight Tropical Macro algae Along Kachchh Coast, India as Human Food Supplements

Journal of Aquatic Food Product Technology, 2017

Research paper thumbnail of Non-Targeted Secondary Metabolite Profile Study for Deciphering the Cosmeceutical Potential of Red Marine Macro Alga Jania rubens—An LCMS-Based Approach

Cosmetics, 2017

This study aims to unveil the cosmeceutical traits of Jania rubens by highlighting its mineral co... more This study aims to unveil the cosmeceutical traits of Jania rubens by highlighting its mineral composition, antioxidant potential, and presence of bioactive molecules using non-targeted metabolite profiling. This study showed that among minerals, (macro), Ca (14790.33 + 1.46 mg/100 g dry weight (DW)) and in (micro) Fe (84.93 + 0.89 mg/100 g DW) was the highest. A total of 23 putative metabolites in the +ESI (Electrospray Ionization) mode of LCMS-TOF (Liquid Chromatography Mass Spectrometry-Time of Flight) were detected. Two anthocyanins-malonylshisonin and 4-demalonylsalvianin (m/z 825.19; anti-aging, antioxidant, anticancer properties) were detected. Two flavonoids, viz, medicocarpin and agecorynin C, 4-O-methylglucoliquiritigenin-a flavonoid-7-O-glycoside, and 5,6,7,8,3 ,4 ,5-heptamethoxyflavone, a polymethoxygenated flavone (m/z 415.15), were detected. Maclurin 3-C-(2 ,3 ,6-trigalloylglucoside) (m/z 863.15) (antioxidant, antimicrobial and anticancer traits) and theaflavonin (m/z 919.18), belonging to the class of theaflavins (whitening and anti-wrinkle agent), were obtained. Pharmacologically active metabolites like berberrubin (m/z 305.1; antitumor activity), icaceine (m/z 358.24; anticonvulsant properties), agnuside (m/z 449.15; constituent for treatment of premenstrual syndrome), γ-coniceine (m/z 108.12; formulations to treat breast cancer), eremopetasitenin B2, and eremosulphoxinolide A (m/z 447.18; therapeutic effect of allergy and asthma) were observed. 6-O-Methylarmillaridin (m/z 445.18) (antimicrobial and antifungal) and simmondsin 2-ferulate, (m/z 534.21) (insecticidal, antifungal and antifeedant) were detected. Aromatic lignans, viz, 8-Acetoxy-4-methoxypinoresinol, sesartemin, and cubebinone (m/z 413.16), in addition to an aromatic terpene glycoside, tsangane L3 glucoside (m/z 357.23), were detected. Zizybeoside I, benzyl gentiobioside, and trichocarposide were also detected. The determination of antioxidant potential was performed through assays such as like DPPH (2,2-diphenyl-1-picrylhydrazyl), FRAP (Ferric Ion Reducing Antioxidant Power), ABTS (2,2-azino-bis(3-ethylbenz-thiazoline-6-sulfonic acid)), and total antioxidants. Therefore, this study progresses the probability for the inclusion of J. rubens as an ingredient in modern day cosmetic formulations.

Research paper thumbnail of Investigation of Solid Supported Dendrimers for Water Disinfection

Research paper thumbnail of Synthesis, characterization and application of green seaweed mediated silver nanoparticles (AgNPs) as antibacterial agents for water disinfection

Water Science and Technology, Jul 2, 2018

A simple and eco-friendly method for the synthesis of hybrid bead silver nanoparticles (AgNPs) em... more A simple and eco-friendly method for the synthesis of hybrid bead silver nanoparticles (AgNPs) employing the aqueous extract derived from natural and renewable source namely tropical benthic green seaweed Ulva flexuosa was developed. This route involves the reduction of Ag+ ions anchored onto macro porous methacrylic acid copolymer beads to AgNPs for employing them as antibacterial agents for in vitro water disinfection. The seaweed extract itself acts as a reducing and stabilizing agent and requires no additional surfactant or capping agent for forming the AgNPs. The nanoparticles were analyzed using high-resolution transmission electron microscopy, UV–Vis spectroscopy, Fourier transform infrared spectroscopy, scanning electron microscopy, energy dispersive X-ray analysis and inductively coupled plasma optical emission spectroscopy. The study elucidates that such biologically synthesized AgNPs exhibit potential antibacterial activity against two Gram positive (Bacillus subtilis, Staphylococcus aureus) and two Gram-negative (Escherichia coli, Pseudomonas aeruginosa) bacterial strains tested. The bacterial count in treated water was reduced to zero for all the strains. Atomic force microscopy was performed to confirm the pre- and post-state of the bacteria with reference to their treatment with AgNPs. Attributes like facile environment-friendly procedure, stability and high antibacterial potency propel the consideration of these AgNPs as promising antibacterial entities.

Research paper thumbnail of Internal Transcribed Spacer (ITS) Region Targeted Molecular Characterization of Macroalgal Diversity Along the Overlooked Expanse of Gulf of Kachchh, India

Proceedings of the National Academy of Sciences, India Section B: Biological Sciences

Research paper thumbnail of Synthesis, characterization and application of green seaweed mediated silver nanoparticles (AgNPs) as antibacterial agents for water disinfection

Water Science and Technology

A simple and eco-friendly method for the synthesis of hybrid bead silver nanoparticles (AgNPs) em... more A simple and eco-friendly method for the synthesis of hybrid bead silver nanoparticles (AgNPs) employing the aqueous extract derived from natural and renewable source namely tropical benthic green seaweed Ulva flexuosa was developed. This route involves the reduction of Ag+ ions anchored onto macro porous methacrylic acid copolymer beads to AgNPs for employing them as antibacterial agents for in vitro water disinfection. The seaweed extract itself acts as a reducing and stabilizing agent and requires no additional surfactant or capping agent for forming the AgNPs. The nanoparticles were analyzed using high-resolution transmission electron microscopy, UV–Vis spectroscopy, Fourier transform infrared spectroscopy, scanning electron microscopy, energy dispersive X-ray analysis and inductively coupled plasma optical emission spectroscopy. The study elucidates that such biologically synthesized AgNPs exhibit potential antibacterial activity against two Gram positive (Bacillus subtilis, St...

Research paper thumbnail of Non-targeted metabolomics approach to assess the brown marine macroalga Dictyota dichotoma as a functional food using liquid chromatography with mass spectrometry

Separation Science Plus , Feb 2020

The study elucidates presence of 16 potential putative metabolites in Dictyota dichotoma through ... more The study elucidates presence of 16 potential putative metabolites in Dictyota dichotoma through non-targeted secondary metabolite profile analysis using electrospray ionization negative mode of liquid chromatography with time-offlight mass spectrometry. Two fatty acyl glycosides viz; 5-megastigmen-7-yne-3,9diol 9-glucoside (biomarker for food consumption) and (3S,7E,9S)-9-hydroxy-4,7megastigmadien-3-one 9-glucoside (nutrient, emulsifier, surfactant), an o-glycosyl compound 5-megastigmen-7-yne-3,9-diol 3-glucoside (biomarker for food consumption), all with m/z value 370.19 were detected. An acyclic monoterpenoid and diterpenoid viz; isodigeranyl and digeranyl (both with m/z 274.26; food biomarkers) were detected. Additionally, (3 ′ x,5 ′ a,9 ′ x,10 ′ b)-O-(3-hydroxy-6-oxo-7-drimen-11-yl) umbelliferone (m/z 396.19; nutrient, emulsifier, surfactant) and kamolonol (m/z 398.2; antibacterial, anti-tumor) were observed. Two angular pyranocoumarins: mammea C/AB cyclo D (m/z 398.2; food consumption biomarker) and 5-hydroxy-8, 8-dimethyl-6-(3-methyl-1-oxobutyl)-4-pentyl-2H,8H-benzo[1,2-b:3,4-b']dipyran-2-one (m/z 398.2; fruit constituent) were present. Two terpene lactones -galbanic acid (m/z 398.2; antimicrobial, anticoagulant, antiviral, anti-inflammatory, anti-cancer) and asacoumarin A (m/z 398.2; antiprotozoal, antiglycation activity) were detected. Armillaribin (m/z 398.2; food biomarker) along with glycinoeclepin B (m/z 558.28; nutrient, emulsifier, surfactant) was noted. Two flavonoids;

Research paper thumbnail of Synthesis, characterization and application of green seaweed mediated silver nanoparticles (AgNPs) as antibacterial agents for water disinfection

Water Science and Technology, 2018

A simple and eco-friendly method for the synthesis of hybrid bead silver nanoparticles (AgNPs) em... more A simple and eco-friendly method for the synthesis of hybrid bead silver nanoparticles (AgNPs) employing the aqueous extract derived from natural and renewable source namely tropical benthic green seaweed Ulva flexuosa was developed. This route involves the reduction of Ag þ ions anchored onto macro porous methacrylic acid copolymer beads to AgNPs for employing them as antibacterial agents for in vitro water disinfection. The seaweed extract itself acts as a reducing and stabilizing agent and requires no additional surfactant or capping agent for forming the AgNPs.

Research paper thumbnail of Non-Targeted Secondary Metabolite Profile Study for Deciphering the Cosmeceutical Potential of Red Marine Macro Alga Jania rubens-An LCMS- Based Approach

Cosmetics, 2017

This study aims to unveil the cosmeceutical traits of Jania rubens by highlighting its mineral co... more This study aims to unveil the cosmeceutical traits of Jania rubens by highlighting its mineral composition, antioxidant potential, and presence of bioactive molecules using non-targeted metabolite profiling. This study showed that among minerals, (macro), Ca (14790.33 + 1.46 mg/100 g dry weight (DW)) and in (micro) Fe (84.93 + 0.89 mg/100 g DW) was the highest.

Research paper thumbnail of Assessment of the Nutritive, Biochemical, Antioxidant and Antibacterial Potential of Eight Tropical Macro algae Along Kachchh Coast, India as Human Food Supplements View supplementary material

Journal of Aquatic Food Product Technology, 2017

Eight tropical marine macro algae were investigated for in-vitro antioxidant, antibacterial, and ... more Eight tropical marine macro algae were investigated for in-vitro antioxidant, antibacterial, and biochemical properties. The moisture content [% DW (dry weight)] ranged from 7.21 to 14.72%; ash, 24.92 to 47.04%; lipids, 0.73 to 2.67%; protein, 4.56 to 12.59%; and carbohydrate, 30.1 to 48.51%. The % sum of polyunsaturated fatty acids (PUFAs) ranged from 21.71 to 78.22%, while omega-6/omega-3 (ω6/ω3) ratio was from 0 to 2.08, which remained within the prescribed World Health Organization (WHO) standards (<10). The % sum of essential amino acids (EAAs) ranged from 86.18 to 204.66, and Na/K ratio ranged from 0.37 to 2.85. The extracts exhibited significant (P < 0.05) values for total phenol (7.25-16.0 µg PGE mg -1 DW) and total flavonoid (4.06-15.63 µg QE mg -1 DW) at 200 µg/ml. Antibacterial activity was tested against the selected food spoilage and pathogenic bacteria. The principal component analysis (PCA) confirmed a positive correlation between total phenolic content, 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) radical-scavenging activities, and ferric reducing antioxidant power (FRAP), which supports the integration of seaweeds as ingredients in functional foods.

Research paper thumbnail of Investigation of solid supported dendrimers for water disinfection

Journal of Applied Polymer Science, 2012

Polystyrene copolymer beads supported dendrimer was synthesized and investigated for its biologic... more Polystyrene copolymer beads supported dendrimer was synthesized and investigated for its biological applications. Macroporous cross-linked polystyrene copolymer beads were synthesized using suspension polymerization. Two successive generations of di(chloroethyl) amine-type end group functionality was formed on the polystyrene copolymer beads. Elemental analysis, Fourier transform infrared spectra, and solid state 13 C NMR spectra were employed to characterize the polymer bound dendrimer. The polymer bound dendrimer was tested for antibacterial action against both Gram-positive and Gram-negative bacteria. The activity against both types of organism increased with an increase in the nitrogen atoms in the polymer back bone. The dendritic structure containing both amino and di(chloroethyl) groups showed significant reduction in the bacterial count when kept in 20 mL autoclaved water with bacterial cultures having an initial count in the range of 12-83 Â 10 6 CFU/mL.

Research paper thumbnail of Polymeric microspheres containing silver nanoparticles as a bactericidal agent for water disinfection

Water Research, 2010

A facile methodology has been developed by anchoring silver nanoparticles on to the macroporous m... more A facile methodology has been developed by anchoring silver nanoparticles on to the macroporous methacrylic acid copolymer beads for disinfection of water in this study.

Research paper thumbnail of Amidoxime copolymer beads containing Cu/Cu 2 O microparticles as a biocidal material for water disinfection

Journal of Applied Polymer Science, 2013

Macroporous crosslinked acrylonitrile-divinylbenzene copolymer beads were synthesized by suspensi... more Macroporous crosslinked acrylonitrile-divinylbenzene copolymer beads were synthesized by suspension polymerization technique. The beads were chemically modified to have amidoxime functional group, which was used as a solid support for anchoring copper microparticles. The copper ions loaded on the copolymer beads were reduced using strong reducing agent to have copper microparticles on the amidoxime copolymer beads. The size of copper particles formed depends upon the amount of copper ions loaded on the beads. The formation of copper microparticles on the copolymer was confirmed by instrumental analysis. The copper containing amidoxime copolymer beads were investigated for the biocidal activity. The size and the distribution of copper particles on the amidoxime copolymer beads influenced their biocidal activity. The biocidal activity was tested against two Gram-positive bacteria, Bacillus subtilis and Staphylococcus aureus, and against two Gram-negative bacteria, Pseudomonas aeruginosa and Escherichia coli. The beads containing copper particles showed better biocidal activity against the Gram-negative bacteria when compared with the Gram-positive organisms.

Research paper thumbnail of A sustainable synthesis of a CuO@C nanocomposite for the remediation of organic dyes in water and its antibacterial properties

Nano-structures & nano-objects, May 1, 2024

Research paper thumbnail of Omics Approaches for Algal Applications

Springer eBooks, Nov 30, 2023

Research paper thumbnail of Evaluation of Tropical Edible Seaweeds Across the Untapped Frontier of Gok for Boosting the Expanse’s Fiscal Valorization

Proceedings of the National Academy of Sciences, India Section B: Biological Sciences

Research paper thumbnail of Untargeted secondary metabolite profiling and putative pathway deduction of a marine Ochrophyte – Iyengaria stellata (Børgesen) using liquid chromatography-mass spectrometry

Research paper thumbnail of Recent Advances in Biotechnology of Seaweeds: An Overview

Sustainable Global Resources Of Seaweeds Volume 1, 2022

Research paper thumbnail of Synthesis, characterization and application of green seaweed mediated silver nanoparticles (AgNPs) as antibacterial agents for water disinfection

A simple and eco-friendly method for the synthesis of hybrid bead silver nanoparticles (AgNPs) em... more A simple and eco-friendly method for the synthesis of hybrid bead silver nanoparticles (AgNPs) employing the aqueous extract derived from natural and renewable source namely tropical benthic green seaweed Ulva flexuosa was developed. This route involves the reduction of Ag+ ions anchored onto macro porous methacrylic acid copolymer beads to AgNPs for employing them as antibacterial agents for in vitro water disinfection. The seaweed extract itself acts as a reducing and stabilizing agent and requires no additional surfactant or capping agent for forming the AgNPs. The nanoparticles were analyzed using high-resolution transmission electron microscopy, UV-Vis spectroscopy, Fourier transform infrared spectroscopy, scanning electron microscopy, energy dispersive X-ray analysis and inductively coupled plasma optical emission spectroscopy. The study elucidates that such biologically synthesized AgNPs exhibit potential antibacterial activity against two Gram positive (Bacillus subtilis, St...

Research paper thumbnail of Non‐targeted metabolomics approach to assess the brown marine macroalga Dictyota dichotoma as a functional food using liquid chromatography with mass spectrometry

SEPARATION SCIENCE PLUS, 2020

Research paper thumbnail of Assessment of the Nutritive, Biochemical, Antioxidant and Antibacterial Potential of Eight Tropical Macro algae Along Kachchh Coast, India as Human Food Supplements

Journal of Aquatic Food Product Technology, 2017

Research paper thumbnail of Non-Targeted Secondary Metabolite Profile Study for Deciphering the Cosmeceutical Potential of Red Marine Macro Alga Jania rubens—An LCMS-Based Approach

Cosmetics, 2017

This study aims to unveil the cosmeceutical traits of Jania rubens by highlighting its mineral co... more This study aims to unveil the cosmeceutical traits of Jania rubens by highlighting its mineral composition, antioxidant potential, and presence of bioactive molecules using non-targeted metabolite profiling. This study showed that among minerals, (macro), Ca (14790.33 + 1.46 mg/100 g dry weight (DW)) and in (micro) Fe (84.93 + 0.89 mg/100 g DW) was the highest. A total of 23 putative metabolites in the +ESI (Electrospray Ionization) mode of LCMS-TOF (Liquid Chromatography Mass Spectrometry-Time of Flight) were detected. Two anthocyanins-malonylshisonin and 4-demalonylsalvianin (m/z 825.19; anti-aging, antioxidant, anticancer properties) were detected. Two flavonoids, viz, medicocarpin and agecorynin C, 4-O-methylglucoliquiritigenin-a flavonoid-7-O-glycoside, and 5,6,7,8,3 ,4 ,5-heptamethoxyflavone, a polymethoxygenated flavone (m/z 415.15), were detected. Maclurin 3-C-(2 ,3 ,6-trigalloylglucoside) (m/z 863.15) (antioxidant, antimicrobial and anticancer traits) and theaflavonin (m/z 919.18), belonging to the class of theaflavins (whitening and anti-wrinkle agent), were obtained. Pharmacologically active metabolites like berberrubin (m/z 305.1; antitumor activity), icaceine (m/z 358.24; anticonvulsant properties), agnuside (m/z 449.15; constituent for treatment of premenstrual syndrome), γ-coniceine (m/z 108.12; formulations to treat breast cancer), eremopetasitenin B2, and eremosulphoxinolide A (m/z 447.18; therapeutic effect of allergy and asthma) were observed. 6-O-Methylarmillaridin (m/z 445.18) (antimicrobial and antifungal) and simmondsin 2-ferulate, (m/z 534.21) (insecticidal, antifungal and antifeedant) were detected. Aromatic lignans, viz, 8-Acetoxy-4-methoxypinoresinol, sesartemin, and cubebinone (m/z 413.16), in addition to an aromatic terpene glycoside, tsangane L3 glucoside (m/z 357.23), were detected. Zizybeoside I, benzyl gentiobioside, and trichocarposide were also detected. The determination of antioxidant potential was performed through assays such as like DPPH (2,2-diphenyl-1-picrylhydrazyl), FRAP (Ferric Ion Reducing Antioxidant Power), ABTS (2,2-azino-bis(3-ethylbenz-thiazoline-6-sulfonic acid)), and total antioxidants. Therefore, this study progresses the probability for the inclusion of J. rubens as an ingredient in modern day cosmetic formulations.

Research paper thumbnail of Investigation of Solid Supported Dendrimers for Water Disinfection

Research paper thumbnail of Synthesis, characterization and application of green seaweed mediated silver nanoparticles (AgNPs) as antibacterial agents for water disinfection

Water Science and Technology, Jul 2, 2018

A simple and eco-friendly method for the synthesis of hybrid bead silver nanoparticles (AgNPs) em... more A simple and eco-friendly method for the synthesis of hybrid bead silver nanoparticles (AgNPs) employing the aqueous extract derived from natural and renewable source namely tropical benthic green seaweed Ulva flexuosa was developed. This route involves the reduction of Ag+ ions anchored onto macro porous methacrylic acid copolymer beads to AgNPs for employing them as antibacterial agents for in vitro water disinfection. The seaweed extract itself acts as a reducing and stabilizing agent and requires no additional surfactant or capping agent for forming the AgNPs. The nanoparticles were analyzed using high-resolution transmission electron microscopy, UV–Vis spectroscopy, Fourier transform infrared spectroscopy, scanning electron microscopy, energy dispersive X-ray analysis and inductively coupled plasma optical emission spectroscopy. The study elucidates that such biologically synthesized AgNPs exhibit potential antibacterial activity against two Gram positive (Bacillus subtilis, Staphylococcus aureus) and two Gram-negative (Escherichia coli, Pseudomonas aeruginosa) bacterial strains tested. The bacterial count in treated water was reduced to zero for all the strains. Atomic force microscopy was performed to confirm the pre- and post-state of the bacteria with reference to their treatment with AgNPs. Attributes like facile environment-friendly procedure, stability and high antibacterial potency propel the consideration of these AgNPs as promising antibacterial entities.

Research paper thumbnail of Internal Transcribed Spacer (ITS) Region Targeted Molecular Characterization of Macroalgal Diversity Along the Overlooked Expanse of Gulf of Kachchh, India

Proceedings of the National Academy of Sciences, India Section B: Biological Sciences

Research paper thumbnail of Synthesis, characterization and application of green seaweed mediated silver nanoparticles (AgNPs) as antibacterial agents for water disinfection

Water Science and Technology

A simple and eco-friendly method for the synthesis of hybrid bead silver nanoparticles (AgNPs) em... more A simple and eco-friendly method for the synthesis of hybrid bead silver nanoparticles (AgNPs) employing the aqueous extract derived from natural and renewable source namely tropical benthic green seaweed Ulva flexuosa was developed. This route involves the reduction of Ag+ ions anchored onto macro porous methacrylic acid copolymer beads to AgNPs for employing them as antibacterial agents for in vitro water disinfection. The seaweed extract itself acts as a reducing and stabilizing agent and requires no additional surfactant or capping agent for forming the AgNPs. The nanoparticles were analyzed using high-resolution transmission electron microscopy, UV–Vis spectroscopy, Fourier transform infrared spectroscopy, scanning electron microscopy, energy dispersive X-ray analysis and inductively coupled plasma optical emission spectroscopy. The study elucidates that such biologically synthesized AgNPs exhibit potential antibacterial activity against two Gram positive (Bacillus subtilis, St...

Research paper thumbnail of Non-targeted metabolomics approach to assess the brown marine macroalga Dictyota dichotoma as a functional food using liquid chromatography with mass spectrometry

Separation Science Plus , Feb 2020

The study elucidates presence of 16 potential putative metabolites in Dictyota dichotoma through ... more The study elucidates presence of 16 potential putative metabolites in Dictyota dichotoma through non-targeted secondary metabolite profile analysis using electrospray ionization negative mode of liquid chromatography with time-offlight mass spectrometry. Two fatty acyl glycosides viz; 5-megastigmen-7-yne-3,9diol 9-glucoside (biomarker for food consumption) and (3S,7E,9S)-9-hydroxy-4,7megastigmadien-3-one 9-glucoside (nutrient, emulsifier, surfactant), an o-glycosyl compound 5-megastigmen-7-yne-3,9-diol 3-glucoside (biomarker for food consumption), all with m/z value 370.19 were detected. An acyclic monoterpenoid and diterpenoid viz; isodigeranyl and digeranyl (both with m/z 274.26; food biomarkers) were detected. Additionally, (3 ′ x,5 ′ a,9 ′ x,10 ′ b)-O-(3-hydroxy-6-oxo-7-drimen-11-yl) umbelliferone (m/z 396.19; nutrient, emulsifier, surfactant) and kamolonol (m/z 398.2; antibacterial, anti-tumor) were observed. Two angular pyranocoumarins: mammea C/AB cyclo D (m/z 398.2; food consumption biomarker) and 5-hydroxy-8, 8-dimethyl-6-(3-methyl-1-oxobutyl)-4-pentyl-2H,8H-benzo[1,2-b:3,4-b']dipyran-2-one (m/z 398.2; fruit constituent) were present. Two terpene lactones -galbanic acid (m/z 398.2; antimicrobial, anticoagulant, antiviral, anti-inflammatory, anti-cancer) and asacoumarin A (m/z 398.2; antiprotozoal, antiglycation activity) were detected. Armillaribin (m/z 398.2; food biomarker) along with glycinoeclepin B (m/z 558.28; nutrient, emulsifier, surfactant) was noted. Two flavonoids;

Research paper thumbnail of Synthesis, characterization and application of green seaweed mediated silver nanoparticles (AgNPs) as antibacterial agents for water disinfection

Water Science and Technology, 2018

A simple and eco-friendly method for the synthesis of hybrid bead silver nanoparticles (AgNPs) em... more A simple and eco-friendly method for the synthesis of hybrid bead silver nanoparticles (AgNPs) employing the aqueous extract derived from natural and renewable source namely tropical benthic green seaweed Ulva flexuosa was developed. This route involves the reduction of Ag þ ions anchored onto macro porous methacrylic acid copolymer beads to AgNPs for employing them as antibacterial agents for in vitro water disinfection. The seaweed extract itself acts as a reducing and stabilizing agent and requires no additional surfactant or capping agent for forming the AgNPs.

Research paper thumbnail of Non-Targeted Secondary Metabolite Profile Study for Deciphering the Cosmeceutical Potential of Red Marine Macro Alga Jania rubens-An LCMS- Based Approach

Cosmetics, 2017

This study aims to unveil the cosmeceutical traits of Jania rubens by highlighting its mineral co... more This study aims to unveil the cosmeceutical traits of Jania rubens by highlighting its mineral composition, antioxidant potential, and presence of bioactive molecules using non-targeted metabolite profiling. This study showed that among minerals, (macro), Ca (14790.33 + 1.46 mg/100 g dry weight (DW)) and in (micro) Fe (84.93 + 0.89 mg/100 g DW) was the highest.

Research paper thumbnail of Assessment of the Nutritive, Biochemical, Antioxidant and Antibacterial Potential of Eight Tropical Macro algae Along Kachchh Coast, India as Human Food Supplements View supplementary material

Journal of Aquatic Food Product Technology, 2017

Eight tropical marine macro algae were investigated for in-vitro antioxidant, antibacterial, and ... more Eight tropical marine macro algae were investigated for in-vitro antioxidant, antibacterial, and biochemical properties. The moisture content [% DW (dry weight)] ranged from 7.21 to 14.72%; ash, 24.92 to 47.04%; lipids, 0.73 to 2.67%; protein, 4.56 to 12.59%; and carbohydrate, 30.1 to 48.51%. The % sum of polyunsaturated fatty acids (PUFAs) ranged from 21.71 to 78.22%, while omega-6/omega-3 (ω6/ω3) ratio was from 0 to 2.08, which remained within the prescribed World Health Organization (WHO) standards (<10). The % sum of essential amino acids (EAAs) ranged from 86.18 to 204.66, and Na/K ratio ranged from 0.37 to 2.85. The extracts exhibited significant (P < 0.05) values for total phenol (7.25-16.0 µg PGE mg -1 DW) and total flavonoid (4.06-15.63 µg QE mg -1 DW) at 200 µg/ml. Antibacterial activity was tested against the selected food spoilage and pathogenic bacteria. The principal component analysis (PCA) confirmed a positive correlation between total phenolic content, 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) radical-scavenging activities, and ferric reducing antioxidant power (FRAP), which supports the integration of seaweeds as ingredients in functional foods.

Research paper thumbnail of Investigation of solid supported dendrimers for water disinfection

Journal of Applied Polymer Science, 2012

Polystyrene copolymer beads supported dendrimer was synthesized and investigated for its biologic... more Polystyrene copolymer beads supported dendrimer was synthesized and investigated for its biological applications. Macroporous cross-linked polystyrene copolymer beads were synthesized using suspension polymerization. Two successive generations of di(chloroethyl) amine-type end group functionality was formed on the polystyrene copolymer beads. Elemental analysis, Fourier transform infrared spectra, and solid state 13 C NMR spectra were employed to characterize the polymer bound dendrimer. The polymer bound dendrimer was tested for antibacterial action against both Gram-positive and Gram-negative bacteria. The activity against both types of organism increased with an increase in the nitrogen atoms in the polymer back bone. The dendritic structure containing both amino and di(chloroethyl) groups showed significant reduction in the bacterial count when kept in 20 mL autoclaved water with bacterial cultures having an initial count in the range of 12-83 Â 10 6 CFU/mL.

Research paper thumbnail of Polymeric microspheres containing silver nanoparticles as a bactericidal agent for water disinfection

Water Research, 2010

A facile methodology has been developed by anchoring silver nanoparticles on to the macroporous m... more A facile methodology has been developed by anchoring silver nanoparticles on to the macroporous methacrylic acid copolymer beads for disinfection of water in this study.

Research paper thumbnail of Amidoxime copolymer beads containing Cu/Cu 2 O microparticles as a biocidal material for water disinfection

Journal of Applied Polymer Science, 2013

Macroporous crosslinked acrylonitrile-divinylbenzene copolymer beads were synthesized by suspensi... more Macroporous crosslinked acrylonitrile-divinylbenzene copolymer beads were synthesized by suspension polymerization technique. The beads were chemically modified to have amidoxime functional group, which was used as a solid support for anchoring copper microparticles. The copper ions loaded on the copolymer beads were reduced using strong reducing agent to have copper microparticles on the amidoxime copolymer beads. The size of copper particles formed depends upon the amount of copper ions loaded on the beads. The formation of copper microparticles on the copolymer was confirmed by instrumental analysis. The copper containing amidoxime copolymer beads were investigated for the biocidal activity. The size and the distribution of copper particles on the amidoxime copolymer beads influenced their biocidal activity. The biocidal activity was tested against two Gram-positive bacteria, Bacillus subtilis and Staphylococcus aureus, and against two Gram-negative bacteria, Pseudomonas aeruginosa and Escherichia coli. The beads containing copper particles showed better biocidal activity against the Gram-negative bacteria when compared with the Gram-positive organisms.