VIOLET KASABRI | The University Of Jordan (original) (raw)

Papers by VIOLET KASABRI

Jordan Journal of Pharmaceutical Sciences, Jul 24, 2023

Hormone Molecular Biology and Clinical Investigation, Nov 29, 2018

Food and Nutrition Sciences, 2013

Research Square (Research Square), Mar 13, 2023

Bratislavské lekárske listy, 2019

12th European Congress of Endocrinology, Apr 1, 2010

European journal of medicinal plants, Jan 10, 2014

Romanian Journal of Diabetes Nutrition and Metabolic Diseases, Sep 30, 2020

Introduction: Osteocalcin (OCN) and Sirtuin 1 (SIRT1) are intricately involved in metabolic syndr... more Introduction: Osteocalcin (OCN) and Sirtuin 1 (SIRT1) are intricately involved in metabolic syndrome (MetS) and prediabetes (PreDM) anomalies and derangements. In a heterogeneous pool of nondiabetic and preDM MetS recruits, adiposity, atherogenicity and blood indices, SIRT1 and OCN were compared to the respective parameters in normoglycemic and lean controls. Further testing of putative relationships between indices and markers was performed in 59 patients with MetS. Material and Methods: In this cross-sectional study, comparisons and correlations were undertaken for biomarkers, adiposity, atherogenicity and hematological indices in 29 MetS-normoglycemic and 30 newly diagnosed drug-naive MetS-preDM patients versus 29 lean, healthy and normoglycemic controls. ANOVA and Spearman rank correlations were used for statistical comparisons. Results: OCN level (OCN; ng/mL) was significantly higher in normoglycemic MetS vs. both MetS-PreDM and controls (28.13±1.22 and 26.02±3.2 vs. 23.3±3.19, P<0.001 respectively). In contrast, the circulating level of SIRT1 (ng/mL) was lower in both normoglycemic and preDM MetS vs. healthy controls (1.42±0.47 and 1.64±0.58 vs. 3.88±0.95; P<0.001, respectively). Except for fasting plasma glucose and glycated hemoglobin (A1C), no further intergroup discrepancy could be identified between normoglycemic-MetS and preDM-MetS. Notably, adiposity indices and the atherogenicity index of plasma were significantly higher in both MetS (normoglycemic and preDM) groups vs. controls. The LDL-C/HDL-C ratio, visceral adiposity index, and waist/hip ratio were higher only in MetS-preDM vs. controls. In the MetS pool (n=59), OCT, but not SIRT1, was associated reciprocally with fasting glycemia and A1C, monocyte/lymphocyte ratio, but proportionally with HC. In the same MetS pool, SIRT1 correlated significantly positively with TG, lipid accumulation product, visceral adiposity index and the atherogenicity index of plasma. Conclusions: OCN and SIRT1 may reciprocally participate in the development of MetS and preDM; both biomarkers may be putatively surrogate diagnostic/prognostic tools for metabolic anomalies prediction/prevention and pharmacotherapy.

Asian Pacific Journal of Cancer Prevention

Jordan Medical Journal, 2017

Metabolic syndrome (Mets) risk factor biomarkers, namely oxytocin (OXT), omentin-1 and irisin, pl... more Metabolic syndrome (Mets) risk factor biomarkers, namely oxytocin (OXT), omentin-1 and irisin, plasma levels were evaluated via colorimetric enzymatic bioassays. A total of 195 Mets patients were recruited from the outpatients’ diabetes and endocrinology clinics at the National Center for Diabetes Endocrinology and Genetics. Participants were subdivided according to their fasting glycemia status into either the normoglycemic subjects group (Mets-controls) or dysglycemic subjects group (Metspre/ T2DM). Enrolled recruits in both study arms were BMI (body mass index)-, gender- and agematched. Distinctively in the Mets-pre/T2DM group; mean circulating levels of both OXT (pg/mL) and omentin-1 (ng/mL) were significantly lower but mean irisin plasma levels (ng/mL) were substantially higher (p<0.01 vs. respective Mets-controls'). Markedly, in the total pool of Mets-participants, plasma OXT levels correlated inversely with irisin plasma levels but proportionally with omentin-1 plasma ...

Journal of the Saudi Heart Association, 2020

Analytical Chemistry Letters, 2020

New synthetic 36 fluoroquinolones (FQs) have been developed. Their anti-inflammatory and 2,2-diph... more New synthetic 36 fluoroquinolones (FQs) have been developed. Their anti-inflammatory and 2,2-diphenyl-1-picrylhydrazyl (DPPH)-and nitric oxide (NO)-radicals scavenging propensities were delineated in vitro. The anti-inflammation related NO radical scavenging bioactivities of new FQs compounds against lipopolysaccharide (LPS)-prompted NO production in RAW 264.7 macrophages were examined using the Griess assay. Selectively nitro FQ compound 2-AnisCEtA exerted an exceedingly superior anti-inflammatory effects (p<0.001 vs. indomethacin IC 50 value of 103.35±4.4 μM) while 7 nitro FQs, 10 reduced FQs and 9 triazolo FQs were moderately more efficacious than indomethacin. The remaining 9 Compounds could display appreciable anti-inflammatory capacity. Unequivocally their DPPH radical scavenging effects were of substantially lesser efficacy than ascorbic acid. Using 400 μM methylglyoxal as the choice glucotoxicity concentration in RAW264.7 macrophages; it was shown that 18 out of 36 FQs could exhibit an exceedingly more superior than or significantly comparable cytoprotection against methylglyoxal-induced carbonyl toxicity to antiglycation aminoguanidine. Suggestively dual modulation of glycation-inflammation can be linked with FQs lipophilicity-chelating properties. FQs can serve as scaffolds for the development and designing of new druggable deglycation and antiglycation therapeutic candidates via duality of antiglycation-antiinflammatory capacities.

International Journal of Diabetes in Developing Countries, 2018

Hormone Molecular Biology and Clinical Investigation

Objectives In this hypothesis paper we explore the underlying mechanisms for long-COVID and how t... more Objectives In this hypothesis paper we explore the underlying mechanisms for long-COVID and how the oxytocinergic neurones could be infected by SARS-CoV-2 leading to a reduction in plasma oxytocin (OXT). Furthermore, we aim to review the relevance of OXT and hypothalamic function in recovery from long-COVID symptoms and pathology, through exploring the pro-health effects of the OXT neuropeptide. Methods A review of published literature was surveyed using Google Scholar and PubMed. Results Numerous experimental data can be shown to correlate with OXT and long-COVID symptoms and conditions, thus providing strong circumstantial evidence to support our hypothesis. It is postulated that the reduction in plasma OXT due to acute and post-viral damage to the hypothalamus and oxytocinergic neurones contributes to the variable multi-system, remitting and relapsing nature of long-COVID. The intranasal route of OXT application was determined to be most appropriate and clinically relevant for th...

Epidemiology and Infection, Jan 23, 2013

The prevalence of natural carriage and molecular epidemiology of methicillin-resistant Staphyloco... more The prevalence of natural carriage and molecular epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant coagulase-negative staphylococci (MR-CoNS) isolates in a Jordanian community were investigated. The MRSA nasal carriage rate in 227 healthy volunteers was 7. 5 % and the majority (81 %) of MRSA harboured the resistance element SCCmec type IVe and were of a novel spa type t9519 (76 %) ; other significant spa gene types were t223 (14. 7%) and t044 (5. 9 %). All MRSA isolates were susceptible to other classes of antibiotics, and tested positive for at least three virulence factor encoding genes, but only two harboured the pvl gene. MR-CoNS carriage was 54. 2% and these isolates were characterized by single, double and untypable SCCmec elements, with Staphylococcus epidermidis SCCmec type IVa predominating. Of eight subjects with nasal co-colonization of MR-CoNS+MRSA, three shared SCCmec type IV in both groups of organisms. This is the first report of methicillin-resistant staphylococci carriage in a Jordanian community and its findings are important for epidemiological study and infection control measures of these organisms.

PubMed, 2014

The ability of hesperidin (HP) to form complexes with five metals; cobalt, nickel, zinc, calcium ... more The ability of hesperidin (HP) to form complexes with five metals; cobalt, nickel, zinc, calcium and magnesium was investigated. The complexation was studied using U.V spectroscopic titration, in methanol as well as aqueous buffer solutions (physiological conditions). Potential complexes were studied by IR and NMR spectroscopy, melting point and their solubility were also evaluated. The interaction of HP and its metal complexes with DNA was investigated by U.V spectroscopy. HP and its potential complexes were also tested for their ability to inhibit alpha amylase and alpha glucosidase enzymes. The results indicated that HP can form 1:1 complexes with cobalt, nickel and zinc in methanolic solution but not in aqueous buffers. Both HP and its metal complexes were found to intercalate DNA, at physiological condition, with preference to GC rich sequences. HP-metal complexes appeared to have higher affinity towards poly A DNA than the free HP. Neither HP nor its complexes exhibited antimicrobial activity against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa or Candida albicans. Results showed that HP has little inhibitory action on glucosidase and amylase enzymes with no obvious effect of complexation on the behavior of free HP. In conclusion HP was shown to form 1:complexes with the studied metal in methanol but not in aqueous buffer solutions. In presence of DNA however, complex formation in aqueous solutions seem to be encouraged with differential effect between the complexes and free HP.

Jordan Journal of Pharmaceutical Sciences, Sep 29, 2021

OBJECTIVES: Uric acid (UA) has a role in pathogenesis of several metabolic abnormalities includin... more OBJECTIVES: Uric acid (UA) has a role in pathogenesis of several metabolic abnormalities including insulin resistance (IR) and their related disorders. The aim of this report is to review the available evidences that reveal the association between UA, IR and related disorders via both noninsulin and insulin-based IR indices. METHODS: The published literature was surveyed using Google Scholar and PubMed entering the terms Obesity, UA, IR, Metabolic Syndrome (MetS), Gestational Diabetes (GDM), Polycystic Ovarian Syndrome (PCOS). RESULTS: UA had substantial positive relationships with IR, as well as obesity, MetS, DM, GDM, and PCOS. Evidently UA with a role in oxidative stress, endothelial dysfunction and induction of inflammation may cause IR in totality, the major factor in development of MetS and related diseases. Homeostatic Model Assessment of Insulin Resistance (HOMA-IR), insulin based IR index, correlated positively with UA. Moreover, specifically triglyceride to high density lipoprotein cholesterol ratio (TG/HDL-C), visceral adiposity index (VAI), lipid accumulation product (LAP), triglyceride to fasting glucose (TyG) index are noninsulin-based IR indices of positive correlations with UA. MetS score for IR (MetS-IR), a non-insulin based IR index, had significantly proportional correlations with MetS components as well as UA level. UA to HDL-cholesterol ratio (UHR) was a pronounced statistical predictor of MetS and diabetes control. UA positively associated with hyperinsulinemia and IR in prediabetes. CONCLUSION: Succinctly UA can be an emerging biochemistry marker of predictive role in IR, MetS and related anomalies. More hyperuricemia related studies are warranted to be oriented from being correlational to mechanistic.

Journal of Pharmaceutical Health Services Research, Oct 14, 2022

Anti-cancer Agents in Medicinal Chemistry, Nov 1, 2022

Background: Incidence rates and prevalence of cancer are substantially high globally. New safe th... more Background: Incidence rates and prevalence of cancer are substantially high globally. New safe therapeutic drugs are endorsed to overcome the high toxicity and poor safety profile of clinical anticancer agents. Objectives: As antineoplastic Vosaroxin is a commercial fluoroquinolone (FQ), we hypothesize that superlative antiproliferation activity of lipophilic FQs/TFQs series correlates to their acidic groups and C8-C7 ethylene diamine Chelation Bridge along with bulky dual halogenations. Methods: We tested dual lipophilic- acidic chelating FQs with a genuine potential of antiproliferative propensities based on their dual DPPH- and NO- radicals scavenging biocapacities using cell based – and colorimetric assays vs. respective reference agents as their molecular action mechanism. Results: In this work, 9 lipophilic-acid chelating FQs and their cyclized TriazoloFQs (TFQs) designed to bear 7- dihaloanilino substituents with a special focus on dichlorosubstitutions have been prepared, characterized and screened against breast T47D and MCF7, Pancreatic PANC1, colorectal HT29, cervical HELA, lung A375, skin A549, and Leukaemia K562 cancer cell lines using sulforhodamine B colorimetric bioassay. Parameters including potency, toxicity, and selectivity (potency/toxicity) have been reported along with DPPH- and NO- radicals’ scavenging propensities - as their molecular action mechanism- in comparison to ascorbic acid and indomethacin, respectively. Using Griess assay in lipopolysaccharide (LPS) prompted RAW264.7 macrophages inflammation, IC50 values (μM) in the ascending order of new FQs’ NO scavenging/antiinflammation capacity were 4a < 3a < 4c < indomethacin (23.8 <33.4 < 36 vs. indomethacin’s 124, respectively). Exceptionally unlike the rest, reduced FQ, 4b exhibited remarkably superior DPPH radical scavenging capacity to ascorbic acid (IC50 values (μM) 19.9 vs. 123.9, p < 0.001). In comparison to cisplatin; nitroFQs (3a, 3b and 3c), the reduced FQs (4a, 4b, and 4c) and the TFQs (5a, 5b and 5c) exerted substantial micromolar antiproliferation IC50 values < 50 μM in cervical Hela cancer cells but lacked comparable bioactivity in leukaemia K562. In both breast MCF7 and T47D cancer cell lines, FQs/TFQs 4a < 3a < 5b (respective IC50 values (μM) 0.52 < 22.7 < 24 vs. cisplatin’s 41.8 and 0.03 < 4.8 < 27 vs. cisplatin’s 509), and in both GI system colorectal HT29 and pancreatic PANC1 cancer cells FQs/TFQs 4a < 3a < 5b and 4a< 3a (respective IC50 values (μM) 0.12 < 3.5 < 15.9 vs. cisplatin’s 148 and 1.5 < 10.4 vs. cisplatin’s 25.5), exerted nanomolar-micromolar affinities of antiproliferation potencies < 50μM. Besides in lung A375 cancer cells FQs/TFQs 4c < 4a < 3a and in skin A549 cancer cells 5c < 3c < 4a < 3a < 4c (respective IC50 values (μM) 0.07 < 3.2 < 10.3 vs. cisplatin’s 390 and 0.5 < 2.3 < 3.8 < 8.8 < 17.3 vs. cisplatin’s 107) exhibited nanomolar-micromolar antineoplastic capacities < 50 μM. Their spectrum of selectivity indices for safety in fibroblasts PDL-based 72h incubations was reported. Unequivocally 4b reduction of viability effectiveness linked with its DPPH radical scavenging effects (without a matching antiinflammation effect). Explicitly 4a, 3a and 4c exerted exquisite antiinflammation-selective cytotoxicity duality in vitro. Conclusions: Such a new potential chelation mechanism can explain the pronounced difference in antineoplastic activity of new FQs/TFQs.