Narmin merza | Dohuk - Academia.edu (original) (raw)

Papers by Narmin merza

Zanco Journal of Medical Sciences

Background and objective: Pseudomonas aeruginosa is very a well-documented nosocomial and opportu... more Background and objective: Pseudomonas aeruginosa is very a well-documented nosocomial and opportunistic microorganism, a little challenge is being present with the identification of such pathogen. This study aimed to identify Pseudomonas on genus and species levels by conventional PCR and determine multi-drug resistant isolates. Methods: A total of 180 clinical isolates of Pseudomonas species were recovered from in and outpatients who attended Azadi and Rezgari Teaching hospitals in Duhok and Erbil city from October 2015 to May 2016. These isolates were phenotypically identified using standard microbiological procedures. A total of 100 isolates were randomly selected and confirmed at a molecular level as Pseudomonas spp. Results: By applying genus-specific gyr B2 primer which produced1130bp amplification band and sixty-eight isolates were identified by PCR as P. aueroginosa using species-specific primer for 16S rRNA region which showed 956bpamplicon. Forty-six isolates out of the sixty-eight resembling Pseudomonas aeruginosa were diagnosed as being multi-drug resistant isolates by the disc diffusion method. Conclusion: It can be concluded that multi-drug resistant isolates can pose a serious threat for the hospital-resident patients as increasing numbers of these isolates are being recorded in local settings.

Molecular characterization of ESBL-related bla genes including blaTEM, blaSHV, and blaCTX-M has b... more Molecular characterization of ESBL-related bla genes including blaTEM, blaSHV, and blaCTX-M has been performed for Escherichia coli isolated from urine and collected from three cities in Kurdistan/region/Iraq (Erbil, Sulymani and Duhok). One hundred sixty nine isolates of E. coli have been identified and their production of ESBLs enzymes have been determined using phenotypic methods. All these isolates were successfully amplified producing a single band of the uidA locus in all strains with a molecular weight of about 670bp in order to confirm at molecular level that all these isolates were E. coli. One hundred sixty ESBL E. coli isolates out of 169 appeared to have one or more ESBLs genes accounting for 94.7%. CTX-M constituted the high prevalent type of ESBLs genes compared to the others represented by 94.1% of all isolates in all the three cities of Kurdistan region followed by TEM and SHV in a percentages of 43.8% and 2.5%, respectively. In Duhok, TEM showed the higher prevalenc...

Science Journal of University of Zakho, 2019

Acinetobacter baumannii (A. baumannii) is an opportunistic pathogen and one of the most importnat... more Acinetobacter baumannii (A. baumannii) is an opportunistic pathogen and one of the most importnat Multidrug resistant microorganisms responsible for a vast array of nosocomial infections. 41 (6.8 %) isolates of A. baumannii were obtained from a total of 603 clinical samples of burn and sputum during the period from March 2018 to February 2019. Twenty two isolates (3.6%) of A. baummanni were recovered from burn infections and nineteen isolates (3.2%) from sputum specimens. Identification and characterization of these isolates was accomplished by the aid of selective media (CHROM agar Acinetobacter) and was finally confirmed by VITEK 2 identification system test. Molecular identification utilizing genus and species-specific primers to detect the 16S rRNA and blaOXA-51 was also applied. The Antibiotic resistance testing was done by the Kirby-Bauer disc diffusion method, 24 .4% of the isolates were Multi-Drug Resistance (MDR), while 65.9% were extensive drug resistance (XDR). All isol...

A total of 364 clinical isolates of Escherichia coli were collected and characterized from differ... more A total of 364 clinical isolates of Escherichia coli were collected and characterized from different hospitals in Kurdistan region. Two hundred ninety six of these were isolated from female accounting (81%) whereas 68(19%) of them were recovered from male. One hundred fifty isolates of UPEC (Fifty from each province) were randomly selected and confirmed as E. coli isolates at molecular level by PCR using uidA primer as a species-specific primer producing 670bp DNA fragment for uidA gene. They were assigned into one of the major phylogenetic groups (A, B1, B2 and D). This is based on the presence or absence of genetic markers including: two genes (chuA and yjaA) and a DNA fragment (TSPE4.C2) as proposed by Clermont et al., 2000. It has been found that 85 (56.7%) of these isolates belonged to phylogenetic group B2, 31 (20.7%) belonged to group D, 27 isolates were phylo-group (A) a counting (18%), and only 7 isolates (4.7%) belonged to group B1. Further classification has been done in ...

In this study, 225 isolates of Pseudomonas aeruginosa were recovered from burn wounds in major ho... more In this study, 225 isolates of Pseudomonas aeruginosa were recovered from burn wounds in major hospitals in Duhok and Erbil, Iraq, between April 2015 and September 2015. A total of 136 of these isolates were from men, comprising 60.4% of the total, whereas 89 (39.6%) were recovered from women. One hundred of these isolates were selected (50 from each province of Erbil and Duhok) and subjected to 16 different antibiotics using the disc diffusion method. The isolates showed a high level of resistance to most of the tested antibiotics, with 90% of the isolates being multidrug resistant. Imipenem was considered as the most effective antibiotic against these isolates with a resistant rate of 47%. The genome of all of these isolates were successfully amplified and produced a single band for the 16S rDNA locus with a molecular weight of about 956 base pairs, which was used to confirm, at the molecular level, that all these isolates were indeed P. aeruginosa. The results of the detection of...

In this study, 150 isolates of Uropathogenic Escherichia coli (UPEC) strains have been collected ... more In this study, 150 isolates of Uropathogenic Escherichia coli (UPEC) strains have been collected from different major hospitals in Kurdistan region and showed high resistant rates to most tested antibiotics accounting for 98%. There was no isolate that had demonstrated sensitive to all antibiotics, but they manifested with a wide range of resistance to most tested antibiotics ranging from resistance to just two antibiotics (2%) to resistance to 15 out of 16 tested antibiotics (9.3%). Imipenem was found as the most potent at all other antimicrobial agents with the resistance rate (4.7%), whereas Tetracycline, Ampicillin, Amoxicillin and Amoxicillin / Clavulanic acid had the least effect on UPEC strains with resistant rates of 83.3%, 92.6%, 90.6% and 90% respectively. The resistance patterns of Chloramphenicol, Norfloxacin, Ciprofloxacin and Nalidixic acid were 30%, 48.6%, 52.6% and 78% respectively. Amikacin, Gentamicin, Trimethoprim and Trimethoprim/ sulfamethoxazole antibiotics als...

ZANCO JOURNAL OF PURE AND APPLIED SCIENCES, Jun 11, 2018

The Journal of The University of Duhok

Zanco Journal of Medical Sciences

Background and objective: Pseudomonas aeruginosa is very a well-documented nosocomial and opportu... more Background and objective: Pseudomonas aeruginosa is very a well-documented nosocomial and opportunistic microorganism, a little challenge is being present with the identification of such pathogen. This study aimed to identify Pseudomonas on genus and species levels by conventional PCR and determine multi-drug resistant isolates. Methods: A total of 180 clinical isolates of Pseudomonas species were recovered from in and outpatients who attended Azadi and Rezgari Teaching hospitals in Duhok and Erbil city from October 2015 to May 2016. These isolates were phenotypically identified using standard microbiological procedures. A total of 100 isolates were randomly selected and confirmed at a molecular level as Pseudomonas spp. Results: By applying genus-specific gyr B2 primer which produced1130bp amplification band and sixty-eight isolates were identified by PCR as P. aueroginosa using species-specific primer for 16S rRNA region which showed 956bpamplicon. Forty-six isolates out of the sixty-eight resembling Pseudomonas aeruginosa were diagnosed as being multi-drug resistant isolates by the disc diffusion method. Conclusion: It can be concluded that multi-drug resistant isolates can pose a serious threat for the hospital-resident patients as increasing numbers of these isolates are being recorded in local settings.

Molecular characterization of ESBL-related bla genes including blaTEM, blaSHV, and blaCTX-M has b... more Molecular characterization of ESBL-related bla genes including blaTEM, blaSHV, and blaCTX-M has been performed for Escherichia coli isolated from urine and collected from three cities in Kurdistan/region/Iraq (Erbil, Sulymani and Duhok). One hundred sixty nine isolates of E. coli have been identified and their production of ESBLs enzymes have been determined using phenotypic methods. All these isolates were successfully amplified producing a single band of the uidA locus in all strains with a molecular weight of about 670bp in order to confirm at molecular level that all these isolates were E. coli. One hundred sixty ESBL E. coli isolates out of 169 appeared to have one or more ESBLs genes accounting for 94.7%. CTX-M constituted the high prevalent type of ESBLs genes compared to the others represented by 94.1% of all isolates in all the three cities of Kurdistan region followed by TEM and SHV in a percentages of 43.8% and 2.5%, respectively. In Duhok, TEM showed the higher prevalenc...

Science Journal of University of Zakho, 2019

Acinetobacter baumannii (A. baumannii) is an opportunistic pathogen and one of the most importnat... more Acinetobacter baumannii (A. baumannii) is an opportunistic pathogen and one of the most importnat Multidrug resistant microorganisms responsible for a vast array of nosocomial infections. 41 (6.8 %) isolates of A. baumannii were obtained from a total of 603 clinical samples of burn and sputum during the period from March 2018 to February 2019. Twenty two isolates (3.6%) of A. baummanni were recovered from burn infections and nineteen isolates (3.2%) from sputum specimens. Identification and characterization of these isolates was accomplished by the aid of selective media (CHROM agar Acinetobacter) and was finally confirmed by VITEK 2 identification system test. Molecular identification utilizing genus and species-specific primers to detect the 16S rRNA and blaOXA-51 was also applied. The Antibiotic resistance testing was done by the Kirby-Bauer disc diffusion method, 24 .4% of the isolates were Multi-Drug Resistance (MDR), while 65.9% were extensive drug resistance (XDR). All isol...

A total of 364 clinical isolates of Escherichia coli were collected and characterized from differ... more A total of 364 clinical isolates of Escherichia coli were collected and characterized from different hospitals in Kurdistan region. Two hundred ninety six of these were isolated from female accounting (81%) whereas 68(19%) of them were recovered from male. One hundred fifty isolates of UPEC (Fifty from each province) were randomly selected and confirmed as E. coli isolates at molecular level by PCR using uidA primer as a species-specific primer producing 670bp DNA fragment for uidA gene. They were assigned into one of the major phylogenetic groups (A, B1, B2 and D). This is based on the presence or absence of genetic markers including: two genes (chuA and yjaA) and a DNA fragment (TSPE4.C2) as proposed by Clermont et al., 2000. It has been found that 85 (56.7%) of these isolates belonged to phylogenetic group B2, 31 (20.7%) belonged to group D, 27 isolates were phylo-group (A) a counting (18%), and only 7 isolates (4.7%) belonged to group B1. Further classification has been done in ...

In this study, 225 isolates of Pseudomonas aeruginosa were recovered from burn wounds in major ho... more In this study, 225 isolates of Pseudomonas aeruginosa were recovered from burn wounds in major hospitals in Duhok and Erbil, Iraq, between April 2015 and September 2015. A total of 136 of these isolates were from men, comprising 60.4% of the total, whereas 89 (39.6%) were recovered from women. One hundred of these isolates were selected (50 from each province of Erbil and Duhok) and subjected to 16 different antibiotics using the disc diffusion method. The isolates showed a high level of resistance to most of the tested antibiotics, with 90% of the isolates being multidrug resistant. Imipenem was considered as the most effective antibiotic against these isolates with a resistant rate of 47%. The genome of all of these isolates were successfully amplified and produced a single band for the 16S rDNA locus with a molecular weight of about 956 base pairs, which was used to confirm, at the molecular level, that all these isolates were indeed P. aeruginosa. The results of the detection of...

In this study, 150 isolates of Uropathogenic Escherichia coli (UPEC) strains have been collected ... more In this study, 150 isolates of Uropathogenic Escherichia coli (UPEC) strains have been collected from different major hospitals in Kurdistan region and showed high resistant rates to most tested antibiotics accounting for 98%. There was no isolate that had demonstrated sensitive to all antibiotics, but they manifested with a wide range of resistance to most tested antibiotics ranging from resistance to just two antibiotics (2%) to resistance to 15 out of 16 tested antibiotics (9.3%). Imipenem was found as the most potent at all other antimicrobial agents with the resistance rate (4.7%), whereas Tetracycline, Ampicillin, Amoxicillin and Amoxicillin / Clavulanic acid had the least effect on UPEC strains with resistant rates of 83.3%, 92.6%, 90.6% and 90% respectively. The resistance patterns of Chloramphenicol, Norfloxacin, Ciprofloxacin and Nalidixic acid were 30%, 48.6%, 52.6% and 78% respectively. Amikacin, Gentamicin, Trimethoprim and Trimethoprim/ sulfamethoxazole antibiotics als...

ZANCO JOURNAL OF PURE AND APPLIED SCIENCES, Jun 11, 2018

The Journal of The University of Duhok