GCMS-based metabolomic study in mice with colitis induced by dextran sulfate sodium (original) (raw)

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1Division of Gastroenterology, Department of Internal Medicine, Graduate School of Medicine, Kobe University, Kobe, Japan

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1Division of Gastroenterology, Department of Internal Medicine, Graduate School of Medicine, Kobe University, Kobe, Japan

2The Integrated Center for Mass Spectrometry, Graduate School of Medicine, Kobe University, Kobe, Japan

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1Division of Gastroenterology, Department of Internal Medicine, Graduate School of Medicine, Kobe University, Kobe, Japan

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2The Integrated Center for Mass Spectrometry, Graduate School of Medicine, Kobe University, Kobe, Japan

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Masakazu Shinohara, MD, PhD

2The Integrated Center for Mass Spectrometry, Graduate School of Medicine, Kobe University, Kobe, Japan

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1Division of Gastroenterology, Department of Internal Medicine, Graduate School of Medicine, Kobe University, Kobe, Japan

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1Division of Gastroenterology, Department of Internal Medicine, Graduate School of Medicine, Kobe University, Kobe, Japan

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1Division of Gastroenterology, Department of Internal Medicine, Graduate School of Medicine, Kobe University, Kobe, Japan

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1Division of Gastroenterology, Department of Internal Medicine, Graduate School of Medicine, Kobe University, Kobe, Japan

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1Division of Gastroenterology, Department of Internal Medicine, Graduate School of Medicine, Kobe University, Kobe, Japan

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Received:

11 November 2010

Accepted:

02 December 2010

Published:

01 February 2011

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Yuuki Shiomi, Shin Nishiumi, Makoto Ooi, Naoya Hatano, Masakazu Shinohara, Tomoo Yoshie, Yasuyuki Kondo, Keisuke Furumatsu, Hideyuki Shiomi, Hiromu Kutsumi, Takeshi Azuma, Masaru Yoshida, GCMS-based metabolomic study in mice with colitis induced by dextran sulfate sodium, Inflammatory Bowel Diseases, Volume 17, Issue 11, 1 November 2011, Pages 2261–2274, https://doi.org/10.1002/ibd.21616
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Background

Metabolomics provides data about all the metabolic processes of a cell or organism. So far, the changes that occur in the levels of metabolites during the development of colitis have not been fully elucidated. Here we examined the changes of metabolite levels in the serum and colon tissue of colitis mice using gas chromatography mass spectrometry (GC/MS) with the aim of achieving a detailed understanding of the pathogenesis of inflammatory bowel disease (IBD).

Methods

To induce colitis, C57BL/6J mice were administered 3.0% dextran sulfate sodium (DSS) in their drinking water for 5 days and were subsequently given drinking water alone.

Results

A total of 77 and 92 metabolites were detected in serum and colon tissue, respectively, and among the metabolites the compositions of TCA cycle intermediates and amino acids changed depending on the degree of colitis. Then, partial least square discriminant analysis (PLS-DA), a multiple classification analysis, showed distinct clustering and clear separation of the groups according to the degree of colitis. Furthermore, PLS-DA loadings plots revealed that succinic acid, indole-3-acetic acid, glutamic acid, and glutamine were the main contributors to the separation of each stage of colitis. In addition, it was revealed that supplementation with glutamine, the level of which was significantly decreased in the acute phase of colonic inflammation, attenuated colitis induced by DSS.

Conclusions

Our results suggest that metabolomics is capable of representing the various degrees of colitis, and our findings will aid in the discovery of therapeutic agents for IBD and other inflammatory disorders by metabolomic approaches. (Inflamm Bowel Dis 2011;)

Copyright © 2011 Crohn's & Colitis Foundation of America, Inc.

Topic:

• colitis
• dextran sulfate
• gas chromatography-mass spectrometry
• colon
• glutamine
• mice
• sodium
• metabolites
• metabolomics

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