Hyperactive Transforming Growth Factor‐β1 Signaling Potentiates Skeletal Defects in a Neurofibromatosis Type 1 Mouse Model (original) (raw)
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Herman B. Wells Center for Pediatric Research, Indiana University School of Medicine, Indiana University School of MedicineIndianapolisINUSA
Department of Anatomy and Cell BiologyIndiana University School of MedicineIndianapolisINUSA
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Department of PediatricsIndiana University School of MedicineIndianapolisINUSA
Herman B. Wells Center for Pediatric Research, Indiana University School of Medicine, Indiana University School of MedicineIndianapolisINUSA
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Department of PediatricsIndiana University School of MedicineIndianapolisINUSA
Herman B. Wells Center for Pediatric Research, Indiana University School of Medicine, Indiana University School of MedicineIndianapolisINUSA
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,
Department of PediatricsIndiana University School of MedicineIndianapolisINUSA
Herman B. Wells Center for Pediatric Research, Indiana University School of Medicine, Indiana University School of MedicineIndianapolisINUSA
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,
Department of PediatricsIndiana University School of MedicineIndianapolisINUSA
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,
Department of PediatricsIndiana University School of MedicineIndianapolisINUSA
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Department of PediatricsIndiana University School of MedicineIndianapolisINUSA
Herman B. Wells Center for Pediatric Research, Indiana University School of Medicine, Indiana University School of MedicineIndianapolisINUSA
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Department of PediatricsIndiana University School of MedicineIndianapolisINUSA
Herman B. Wells Center for Pediatric Research, Indiana University School of Medicine, Indiana University School of MedicineIndianapolisINUSA
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Department of Biomedical EngineeringIndiana University–Purdue UniversityIndianapolisINUSA
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Department of Biomedical EngineeringIndiana University–Purdue UniversityIndianapolisINUSA
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Received:
27 February 2013
Revision received:
29 April 2013
Cite
Steven D Rhodes, Xiaohua Wu, Yongzheng He, Shi Chen, Hao Yang, Karl W Staser, Jiapeng Wang, Ping Zhang, Chang Jiang, Hiroki Yokota, Ruizhi Dong, Xianghong Peng, Xianlin Yang, Sreemala Murthy, Mohamad Azhar, Khalid S Mohammad, Mingjiang Xu, Theresa A Guise, Feng‐Chun Yang, Hyperactive Transforming Growth Factor‐β1 Signaling Potentiates Skeletal Defects in a Neurofibromatosis Type 1 Mouse Model, Journal of Bone and Mineral Research, Volume 28, Issue 12, 1 December 2013, Pages 2476–2489, https://doi.org/10.1002/jbmr.1992
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ABSTRACT
Dysregulated transforming growth factor beta (TGF‐β) signaling is associated with a spectrum of osseous defects as seen in Loeys‐Dietz syndrome, Marfan syndrome, and Camurati‐Engelmann disease. Intriguingly, neurofibromatosis type 1 (NF1) patients exhibit many of these characteristic skeletal features, including kyphoscoliosis, osteoporosis, tibial dysplasia, and pseudarthrosis; however, the molecular mechanisms mediating these phenotypes remain unclear. Here, we provide genetic and pharmacologic evidence that hyperactive TGF‐β1 signaling pivotally underpins osseous defects in Nf1 flox/−_;Col2.3Cre_ mice, a model which closely recapitulates the skeletal abnormalities found in the human disease. Compared to controls, we show that serum TGF‐β1 levels are fivefold to sixfold increased both in Nf1 flox/−_;Col2.3Cre_ mice and in a cohort of NF1 patients. _Nf1_‐deficient osteoblasts, the principal source of TGF‐β1 in bone, overexpress TGF‐β1 in a gene dosage–dependent fashion. Moreover, Nf1_‐deficient osteoblasts and osteoclasts are hyperresponsive to TGF‐β1 stimulation, potentiating osteoclast bone resorptive activity while inhibiting osteoblast differentiation. These cellular phenotypes are further accompanied by p21‐Ras–dependent hyperactivation of the canonical TGF‐β1–Smad pathway. Reexpression of the human, full‐length neurofibromin guanosine triphosphatase (GTPase)‐activating protein (GAP)‐related domain (NF1 GRD) in primary Nf1_‐deficient osteoblast progenitors, attenuated TGF‐β1 expression levels and reduced Smad phosphorylation in response to TGF‐β1 stimulation. As an in vivo proof of principle, we demonstrate that administration of the TGF‐β receptor 1 (TβRI) kinase inhibitor, SD‐208, can rescue bone mass deficits and prevent tibial fracture nonunion in Nf1 flox/−;Col2.3Cre mice. In sum, these data demonstrate a pivotal role for hyperactive TGF‐β1 signaling in the pathogenesis of NF1‐associated osteoporosis and pseudarthrosis, thus implicating the TGF‐β signaling pathway as a potential therapeutic target in the treatment of NF1 osseous defects that are refractory to current therapies. © 2013 American Society for Bone and Mineral Research.
© 2013 American Society for Bone and Mineral Research
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