Expression of the human NRAMP1 gene in professional primary phagocytes: studies in blood cells and in HL-60 promyelocytic leukemia (original) (raw)

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Department of Biochemistry, McGill University

, Montreal

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Division of Hematology, Royal Victoria Hospital

, Montreal, Quebec,

Canada

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Arizona Cancer Center, University of Arizona

, Tucson

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Department of Medicine, Case Western Reserve University

, Cleveland, Ohio

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Department of Medicine, McGill University

, Montreal

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Department of Medicine, McGill University

, Montreal

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Department of Medicine, McGill University

, Montreal

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Department of Biochemistry, McGill University

, Montreal

Correspondence: Philippe Gros, Dept. Biochemistry, McGill University, 3655 Drummond, Montreal, Quebec, Canada, H3G-1Y6

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Revision received:

18 September 1996

Accepted:

19 September 1996

Published:

01 January 1997

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Mathieu Cellier, Chaim Shustik, William Dalton, Elizabeth Rich, Jinxin Hu, Danielle Malo, Erwin Schurr, Philippe Gros, Expression of the human NRAMP1 gene in professional primary phagocytes: studies in blood cells and in HL-60 promyelocytic leukemia, Journal of Leukocyte Biology, Volume 61, Issue 1, Jan 1997, Pages 96–105, https://doi.org/10.1002/jlb.61.1.96
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Abstract

In the mouse, mutations at the natural resistance-associated macrophage protein 1 (Nrampl) gene abrogate resistance to infection with antigenically unrelated intracellular parasites such as Mycobacterium, Salmonella, and Leishmania. Nrampl expression is restricted to reticuloendothelial organs and peripheral blood leukocytes, where the protein may function as a membrane transporter of an as yet to be identified substrate. To identify the human blood cell type(s) expressing NRAMPl mRNA and determine how Nrampl expression is regulated in these cells, we have examined separated populations of peripheral blood leukocytes and in vitro cell lines. We observed that polymorphonuclear leukocytes (PMN) are the major site of NRAMP1 expression, followed to a lesser degree by monocytes (MN). Migration of MN to tissues (alveolar macrophages) or maturation in vitro (long-term culture) was associated with a higher level of NRAMP1 expression compared with blood MN. Northern analyses of RNA from model cultured cells showed absence of NRAMP1 expression in transformed cell lines from either erythroid or lymphoid T or B lineages as well as progenitors of the monocyte/macrophage pathway (KG1, U937, THP1), and the HL-60 promyelocytic leukemia. Induction of differentiation of HL-60 cells toward either the monocyte/ macrophage (vitamin D3, phorbol ester) or the granulocyte pathways (DMF, DMSO), as measured by induction of _IL8_-Rb, c-FMS, and CD14 marker gene expression, was concomitant with a strong induction of NRAMP1 expression. These results suggest that NRAMP1 expression is specific to the myeloid lineage and is acquired during the maturation of PMN and MN. The possibility that NRAMP1 may be a component of the phagosomal/endosomal apparatus common to PMN and MN is discussed.

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© 1997 Society for Leukocyte Biology

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