Converting Human Pluripotent Stem Cells to Neural Tissue and Neurons to Model Neurodegeneration (original) (raw)

Abstract

Human embryonic stem cells (hESCs) and the related induced pluripotent stem cells (hiPSCs) have attracted considerable attention since they can provide an unlimited source of many different tissue types. One challenge of using pluripotent cells is directing their broad differentiation potential into one specific tissue or cell fate. The cell fate choices of extraembryonic, endoderm, mesoderm, and ectoderm (including neural) lineages represent the earliest decisions. We found that pluripotent cells efficiently neuralize by blocking the signaling pathways required for alternative cell fate decisions. In this chapter, we detail methods to direct hESCs or hiPSCs into early neural cells and subsequently postmitotic neurons.

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Acknowledgments

The authors would like to thank NYSTEM and The Starr Foundation for support of our research. SMC is a Starr Foundation, Tri-Institute Stem Cell Initiative Fellow.

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Authors and Affiliations

  1. Developmental Biology Program, Sloan-Kettering Institute, New York, NY, USA
    Stuart M. Chambers
  2. Gerstner Sloan-Kettering Graduate School of Biomedical Sciences, Sloan-Kettering Institute, New York, NY, USA
    Yvonne Mica
  3. Developmental Biology Program, Department of Neurosurgery, Sloan-Kettering Institute, New York, NY, USA
    Lorenz Studer
  4. SKI Stem Cell Research Facility, Sloan-Kettering Institute, New York, NY, USA
    Mark J. Tomishima

Authors

  1. Stuart M. Chambers
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  2. Yvonne Mica
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  3. Lorenz Studer
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  4. Mark J. Tomishima
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Corresponding author

Correspondence toMark J. Tomishima .

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Editors and Affiliations

  1. Weill Medical College, Dept. Neurology & Neuroscience, Cornell University, East 68th St. 525, New York, 10065, New York, USA
    Giovanni Manfredi
  2. York Avenue 1300, New York, 10065, New York, USA
    Hibiki Kawamata

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© 2011 Springer Science+Business Media, LLC

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Chambers, S.M., Mica, Y., Studer, L., Tomishima, M.J. (2011). Converting Human Pluripotent Stem Cells to Neural Tissue and Neurons to Model Neurodegeneration. In: Manfredi, G., Kawamata, H. (eds) Neurodegeneration. Methods in Molecular Biology, vol 793. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-328-8\_6

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